KR20170052811A - yeast fermented solutions of green tea extract and manufacturing method thereof and cosmetic composition using the same - Google Patents
yeast fermented solutions of green tea extract and manufacturing method thereof and cosmetic composition using the same Download PDFInfo
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- KR20170052811A KR20170052811A KR1020150154626A KR20150154626A KR20170052811A KR 20170052811 A KR20170052811 A KR 20170052811A KR 1020150154626 A KR1020150154626 A KR 1020150154626A KR 20150154626 A KR20150154626 A KR 20150154626A KR 20170052811 A KR20170052811 A KR 20170052811A
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- KR
- South Korea
- Prior art keywords
- green tea
- yeast
- tea extract
- fermentation broth
- yeast fermentation
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
본 발명은 녹차추출물의 효모발효액 및 이의 제조방법 그리고 이를 이용한 화장료 조성물에 관한 것으로서, 더욱 상세하게는 녹차추출물을 효모로 발효시켜 항산화능을 크게 증대시킨 효모발효액을 제공할 수 있으며 효모발효액을 화장료 조성물의 유효성분으로 활용하여 항산화능이 높고 피부에 저자극인 화장료 조성물을 제조할 수 있는 녹차추출물의 효모발효액 및 이의 제조방법 그리고 이를 이용한 화장료 조성물에 관한 것이다.
본 발명의 녹차추출물의 효모발효액의 제조방법은 녹차에 추출용매를 가해 녹차추출물을 추출하는 추출단계와, 녹차추출물에 효모를 첨가하여 발효시키는 발효단계와, 발효단계에서 수득한 효모발효액을 열처리하여 효모를 실활시키는 실활단계와, 실활단계 후 효모발효액을 원심분리하여 얻은 상등액을 여과하여 잔여물을 제거하는 여과단계를 포함한다.The present invention relates to a yeast fermentation broth of green tea extract, a preparation method thereof, and a cosmetic composition using the same, and more particularly, to a yeast fermentation broth which has greatly enhanced antioxidant ability by fermenting green tea extract with yeast, Which is high in antioxidant ability and can be prepared into a skin cosmetic composition on the skin, a method for producing the yeast fermentation broth, and a cosmetic composition using the same.
A method for producing a yeast fermentation broth of a green tea extract according to the present invention comprises: an extraction step of extracting a green tea extract by adding an extraction solvent to a green tea; a fermentation step of fermenting the green tea extract by adding yeast to the green tea extract; A deactivation step of deactivating the yeast, and a filtration step of filtering the supernatant obtained by centrifuging the yeast fermentation broth after the deactivation step to remove the residue.
Description
본 발명은 녹차추출물의 효모발효액 및 이의 제조방법 그리고 이를 이용한 화장료 조성물에 관한 것으로서, 더욱 상세하게는 녹차추출물을 효모로 발효시켜 항산화능을 크게 증대시킨 효모발효액을 제공할 수 있으며 효모발효액을 화장료 조성물의 유효성분으로 활용하여 항산화능이 높고 피부에 저자극인 화장료 조성물을 제조할 수 있는 녹차추출물의 효모발효액 및 이의 제조방법 그리고 이를 이용한 화장료 조성물에 관한 것이다. The present invention relates to a yeast fermentation broth of green tea extract, a preparation method thereof, and a cosmetic composition using the same, and more particularly, to a yeast fermentation broth which has greatly enhanced antioxidant ability by fermenting green tea extract with yeast, Which is high in antioxidant activity and can be prepared into a skin cosmetic composition on the skin, a process for producing the yeast fermentation broth, and a cosmetic composition using the same.
피부는 신체 중 가장 큰 기관으로 항상 외부환경과 직접 접하고 있으면서 여러 가지 자극이나 건조한 환경으로부터 생체를 보호하는 보호막의 역할을 하고 있으며, 다른 기관에 비하여 새로운 세포의 생성과 소멸이 활발히 일어나는 기관이다. 또한, 물리적인 찰과상으로부터 신체를 보호하고, 신체 내부의 수분의 손실을 막아주며 태양에서 발생되는 자외선으로부터 보호해주고, 몸의 체온을 조절해주는 데 매우 중요한 역할을 담당하고 있다.The skin is the largest organ of the body, always in direct contact with the external environment, and serves as a protective layer for protecting the living body from various stimuli or dry environment. It is an organ where new cells are generated and eliminated extensively as compared with other organs. In addition, it protects the body from physical abrasion, protects the inside of the body from moisture loss, protects it from ultraviolet rays generated from the sun, and plays a very important role in controlling body temperature.
이러한 피부는 여러 가지 물리적 요인, 외부환경적 요인 등으로 인한 외부 자극을 받게 되면 주름 생성, 탄력 손실, 각질화 등과 같은 노화가 발생하게 된다. 피부 노화는 크게 자연 노화(혹은 내인성 노화)와 외적 노화로 구분되며, 자연 노화는 유전적인 요소에 영향을 받기 때문에 인위적인 조절이 어려운 반면 외적 노화는 환경적인 요소에 영향을 받기 때문에 인위적인 조절이 비교적 용이하다.These externalities, such as wrinkles, loss of elasticity, and keratinization, are caused by external stimuli due to various physical and external environmental factors. Skin aging is largely classified into natural aging (or endogenous aging) and external aging. Natural aging is difficult to artificially control because it is affected by genetic factors, whereas external aging is affected by environmental factors. Do.
대표적인 외적 노화 인자로는 자외선을 들 수 있으며, 가장 두드러진 외적인 노화 현상은 주름 형성이다(Daniell HW, Ann. Intern. Med., 1971, 75(6), 873; Grove GL 등, J. Am. Acad. Dermatol., 1989, 21(3), 631; Griffiths CE 등, Arch. Dermatol., 1992, 128, 347). 자외선에 의해 야기되는 광노화 메커니즘 중의 하나는 자유 라디칼 경로를 경유하는 것이다(Harman D, J. Gerontol., 1956, 11(3), 298).Exemplary extrinsic aging factors include ultraviolet light and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch. Dermatol., 1992, 128, 347). One of the photo-aging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298).
정상적인 세포에서도 대사과정 중 어느 정도의 자유 라디칼 (free radical)과 기타 활성산소 및 과산화물이 생성되고 있으나, 생체 내에는 이들에 대한 방어기구로서 슈퍼옥사이드디스뮤타제(SOD), 카탈라아제(catalase), 퍼록시다제 (peroxidase) 등의 항산화성 효소와 함께, 비타민 C, 비타민 E, 글루타치온(glutathione), 유비퀴논(ubiquinone), 요산(uric acid) 등의 항산화 물질이 존재하여 스스로를 보호하고 있다. 그러나, 이와 같은 생체방어기구에 이상이 초래되거나 각종 물리적, 화학적 요인들에 의하여 활성산소의 생성이 생체방어계의 용량을 초과하게 될 경우, 산화적 스트레스(oxidative stress)가 야기된다. 그러므로 활성 산소를 억제하는 것은 피부노화 화장료에 중요한 요소이다.In normal cells, free radicals and other active oxygen and peroxides are produced to some extent during metabolism. In the body, however, superoxide dismutase (SOD), catalase, And antioxidant enzymes such as peroxidase and antioxidants such as vitamin C, vitamin E, glutathione, ubiquinone and uric acid are present to protect themselves. However, oxidative stress is caused when an abnormality occurs in such a biological defense mechanism or when the production of active oxygen by various physical and chemical factors exceeds the capacity of a biological defense system. Therefore, suppression of active oxygen is an important factor in skin aging cosmetics.
이러한 활성 산소종의 중화를 목적으로 토코페롤, 폴리페놀류, Coenzyme Q 10, BHT, BHA 등 항산화제가 폭 넓게 이용되고 있으나 이들은 대부분 합성품으로서 장기간 인간의 피부에 적용할 경우 안전성이 문제화되므로 사용이 제한된다. 이에 따라 항산화 효과가 높고, 사람의 피부에 대해서는 안전성이 보증되며, 경제적인, 식물기원의 천연 항산화제를 개발하고자 하는 많은 연구가 기대 속에 이루어지고 있다.Antioxidants such as tocopherol, polyphenols,
많은 식물 종에는 식물 보호효과가 우수한 폴리페놀류가 다양하게 함유되어 있으므로, 녹차, 상백피, 황금 등 수많은 식물 추출물이 항산화제로서 화장품에 폭 넓게 사용되고 있다. 현재까지 알려진 천연 항산화 물질로는 토코페롤 (tocopherol)류, 플라보노이드(flavonoid)류, 고시폴(gossypol), 세사몰 (sesamol), 오리자놀(oryzanol) 및 비타민 C 등을 들 수 있다.Since many plant species contain various polyphenols with excellent plant protection effect, many plant extracts such as green tea, mallow, and gold are widely used in cosmetics as antioxidants. Natural antioxidants known to date include tocopherols, flavonoids, gossypol, sesamol, oryzanol, and vitamin C, among others.
한편, 발효(fermentation)란 미생물이 분비하는 효소에 의해 유기물을 분해하는 과정을 말한다. 또한 미생물이 에너지를 얻는 과정에서 유용한 물질을 내는 것도 발효이다. 한국인은 이러한 발효와 친숙한데 예로부터 김치를 비롯하여 된장, 청국장 등의 전통 발효식품이 대표적이다. 이러한 발효과정은 무산소호흡을 하는 미생물들로 유기물을 완전히 분해 시키고 다른 종류의 유기물을 만들어 내기도 하여 적은 양의 에너지를 생성시킨다. 이러한 발효의 종류는 미생물에 따라 젖산발효, 알콜 발효, 프로피온산 발효, 메탄 발효 등이 있다. 이러한 발효에 관계되는 미생물의 대표적인 유산균은 포도당 또는 유당과 같은 당을 분해하여 젖산이나 초산과 같은 유기산을 생성하며 부산물로 아밀라아제, 셀룰라아제, 리파아제, 프로테아제와 같은 효소를 생성하여 천연물 내의 거대분자의 저분자화를 통하여 피부침투를 용이하게 해준다.On the other hand, fermentation refers to the process of decomposing organic matter by an enzyme secreted by a microorganism. It is also fermentation that microorganisms produce useful substances in the process of obtaining energy. Traditional Korean fermented foods such as doenjang and cheonggukjang are typical examples of kimchi, as well as familiarity with Koreans. This fermentation process is an anaerobic respiration microorganism that completely decomposes organic matter and produces other kinds of organic matter, thus generating a small amount of energy. These kinds of fermentation are lactic acid fermentation, alcohol fermentation, propionic acid fermentation, and methane fermentation depending on microorganisms. Lactic acid bacteria, which are representative of microorganisms involved in such fermentation, decompose sugars such as glucose or lactose to produce organic acids such as lactic acid and acetic acid, and produce enzymes such as amylase, cellulase, lipase and protease as byproducts, Thereby facilitating skin penetration.
최근 화장품에 적용되는 천연물 소재 추출물은 이들의 효능, 효과를 극대화 시키는 방법으로 발효 기술을 적용하고 있다. 그 중 분열 효모 발효 방법은 영양 성분들을 저분자화 하여 피부 침투를 촉진시키고, 피부 천연보습인자와 유사한 고농도의 아미노산 성분들로 인해 저자극이면서 보습, 주름 등 다양한 효과를 한 번에 느낄 수 있도록 하는 기술로, 기존 한방추출물 소재와 이러한 농축 및 발효 기술을 접목한 신소재 개발이 진행되고 있다.Recently, natural material extracts applied to cosmetics have applied fermentation technology as a method of maximizing their efficacy and effectiveness. Among them, the fermentation yeast fermentation method promotes penetration of the skin by lowering the nutrients and promotes the penetration of the skin. It is a technique to make various effects such as moisturizing and wrinkling at one time because of the high concentration of amino acids similar to the skin moisturizing factor , Development of new materials combining the existing herbal extract material with such enrichment and fermentation technology is underway.
화장품에서의 발효는 미생물을 발효시키는 과정에서 발효균을 제거하는 동시에 효소 외의 유효성분들을 함께 추출함으로써 기존 효소 하나만이 갖는 효능을 배가시키고 함유 성분들을 피부에 빠르게 흡수되도록 한다. 또한 아미노산류, 비타민류, 각종 기능성 물질이 생성되고 합성되는 과정에서 영양소의 파괴는 거의 일어나지 않으며 발효 산물들은 효모가 먹고 배설한 물질이므로 분자구조가 작아져 피부에 흡수가 용이하다(대한피부미용학회지 제8권 제3호 286-287).The fermentation in the cosmetics removes the fermenting bacteria in the process of fermenting the microorganisms and simultaneously extracts the active ingredients other than the enzyme, thereby doubling the effect of the existing enzyme and rapidly absorbing the ingredients into the skin. In addition, amino acids, vitamins, and various functional materials are produced and synthesized, the destruction of nutrients hardly occurs, and the fermentation products are substances that are eaten and excreted by yeast, 8, No. 3, No. 286-287).
천연화장품 발효 소재 시장은 주로 EM유용미생물을 활용한 발효추출물 원료로 주를 이루고 있다. 현재 효모보다 유산균을 활용한 화장료 조성물 위주로 연구가 되는 실정이다. The natural cosmetics fermentation material market is mainly composed of fermented extract materials using EM beneficial microorganisms. Currently, research is focused on cosmetic compositions using lactic acid bacteria rather than yeast.
본 발명은 상기의 문제점을 개선하고자 창출된 것으로서, 녹차추출물을 효모로 발효시켜 항산화능을 크게 증대시킨 효모발효액을 제공할 수 있도록 최적의 발효조건을 확립하고, 이를 통해 제공되는 효모발효액을 화장료 조성물의 유효성분으로 활용하여 항산화능이 높고 피부에 저자극인 화장료 조성물을 제공하는 데 그 목적이 있다. The present invention has been made in order to overcome the above problems, and it is an object of the present invention to provide a yeast fermentation broth having significantly enhanced antioxidative ability by fermenting green tea extract with yeast, establishing optimal fermentation conditions, Which has high antioxidant ability and provides a skin cosmetic composition to the skin.
상기의 목적을 달성하기 위한 본 발명의 녹차추출물의 효모발효액은 녹차추출물을 효모로 발효시켜 상기 녹차추출물의 항산화능을 증대시킨 것을 특징으로 한다.To achieve the above object, the yeast fermentation broth of green tea extract of the present invention is characterized in that the green tea extract is fermented by yeast to increase the antioxidative activity of the green tea extract.
상기 효모는 사카로마이세스 세레비시아(Saccharomyces cerevisiae)인 것을 특징으로 한다.Wherein the yeast is Saccharomyces Levy MY access to three cyano (Saccharomyces cerevisiae .
그리고 상기의 목적을 달성하기 위한 본 발명의 녹차추출물의 효모발효액의 제조방법은 녹차에 추출용매를 가해 녹차추출물을 추출하는 추출단계와; 상기 녹차추출물에 효모를 첨가하여 발효시키는 발효단계와; 상기 발효단계에서 수득한 효모발효액을 열처리하여 효모를 실활시키는 실활단계와; 상기 실활단계 후 상기 효모발효액을 원심분리하여 얻은 상등액을 여과하여 잔여물을 제거하는 여과단계;를 포함한다.According to another aspect of the present invention, there is provided a method for preparing a yeast fermentation broth of a green tea extract, comprising the steps of: extracting a green tea extract by adding an extraction solvent to a green tea; A fermentation step of adding yeast to the green tea extract to ferment; An inactivating step of inactivating the yeast by heat-treating the yeast fermentation broth obtained in the fermentation step; And filtering the supernatant obtained by centrifuging the yeast fermentation broth after the inactivation step to remove the residue.
상기 추출단계는 상기 녹차에 대하여 추출용매를 중량비로 2 내지 40배를 가한 후 10 내지 150℃에서 1 내지 20시간 동안 추출한 후 원심분리하여 상등액을 수득하고, 상기 상등액을 감압여과하여 상기 녹차추출물을 추출한 것을 특징으로 한다. The green tea is extracted with the extraction solvent at a weight ratio of 2 to 40 times at a temperature of 10 to 150 ° C for 1 to 20 hours, and centrifuged to obtain a supernatant. The supernatant is filtered under reduced pressure to remove the green tea extract .
상기 발효단계는 상기 녹차추출물 100mL 당 효모추출물 0.3g, 맥아추출물 0.3g, 펩톤 0.5g, 포도당 1g을 첨가하여 121℃에 15분간 멸균한 후 사카로마이세스 세레비시아(Saccaromyces cerevisiae)를 2%(v/v)가 되게 접종한 후 80rpm으로 34℃에서 72시간 동안 발효시켜 상기 효모발효액을 수득한다.The fermentation step is the green tea extract, yeast extract per 100mL 0.3g, 0.3g malt extract, peptone 0.5g, three to Levy cyano saccharide followed by the addition of glucose, 1g sterilized for 15 minutes in 121 ℃ My process (Saccaromyces cerevisiae to 2% (v / v) and fermented at 80 rpm at 34 DEG C for 72 hours to obtain the yeast fermentation broth.
그리고 상기의 목적을 달성하기 위한 본 발명의 화장료 조성물은 녹차추출물을 효모로 발효시켜 상기 녹차추출물의 항산화능을 증대시킨 효모발효액을 0.1 내지 40중량% 함유하여 화장수, 로션, 크림, 에센스, 팩, 메이크업베이스, 파운데이션, 바디오일, 바디로션, 클렌징오일, 클렌징폼, 헤어오일, 헤어샴푸 및 헤어린스 중에서 선택된 어느 하나의 제형으로 형성되고, 피부에 저자극성인 것을 특징으로 한다.To achieve the above object, the cosmetic composition of the present invention comprises 0.1 to 40% by weight of a yeast fermentation broth which fermented green tea extract as a yeast to increase the antioxidative activity of the green tea extract. The cosmetic composition contains lotion, cream, essence, The composition is formed of any one of a makeup base, a foundation, a body oil, a body lotion, a cleansing oil, a cleansing foam, a hair oil, a hair shampoo and a hair rinse.
본 발명에 의하면 녹차추출물을 효모로 발효시킴으로써 발효 전 녹차추출물에 비해 항산화능을 크게 증대시킬 수 있다. According to the present invention, by fermenting green tea extract with yeast, the antioxidant ability can be greatly increased as compared with the green tea extract before fermentation.
따라서 녹차추출물의 효모발효액을 함유한 화장료 조성물은 항산화활성이 우수하고 피부에 저자극이어서 고기능의 화장료 조성물을 제공할 수 있다. Therefore, the cosmetic composition containing the yeast fermentation broth of green tea extract has excellent antioxidative activity and is hypodermic to the skin, thus providing a high-performance cosmetic composition.
도 1은 녹차추출물, 실시예 1의 효모발효액, 비교예의 효모발효액의 라디칼 소거능을 측정한 결과이고,
도 2는 녹차추출물, 실시예 1의 효모발효액, 비교예의 효모발효액의 총 폴리페놀 함량을 측정한 결과이고,
도 3은 녹차추출물, 실시예 1의 효모발효액, 비교예의 효모발효액의 총 플라보노이드 함량을 측정한 결과이고,
도 4는 실시예 2의 샴푸 및 실시예 3의 바디클렌저의 피부테스트 실험결과 보고서의 요약문이다. Fig. 1 shows the results of measuring the radical scavenging activity of the green tea extract, the yeast fermentation broth of Example 1, and the yeast fermentation broth of the comparative example,
FIG. 2 shows the results of measuring the total polyphenol content of the green tea extract, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example,
FIG. 3 shows the results of measuring the total flavonoid content of the green tea extract, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example,
Fig. 4 is a summary of a skin test result report of the shampoo of Example 2 and the body cleanser of Example 3; Fig.
이하, 본 발명의 바람직한 실시 예에 따른 녹차추출물의 효모발효액 및 이의 제조방법 그리고 이를 이용한 화장료 조성물에 대하여 구체적으로 설명한다. Hereinafter, a yeast fermentation broth of green tea extract according to a preferred embodiment of the present invention, a preparation method thereof, and a cosmetic composition using the same will be described in detail.
본 발명의 녹차추출물의 효모발효액은 녹차추출물을 효모로 발효시켜 얻는다. 이러한 효모발효액은 녹차추출물의 항산화능을 효과적으로 증대시킨다. The yeast fermentation broth of green tea extract of the present invention is obtained by fermenting green tea extract with yeast. This yeast fermentation solution effectively increases the antioxidant ability of green tea extract.
녹차추출물의 효모발효액의 제조방법은 크게 녹차에 추출용매를 가해 녹차추출물을 추출하는 추출단계와, 녹차추출물에 효모를 첨가하여 발효시키는 발효단계와, 효모발효액을 열처리하여 효모를 실활시키는 실활단계와, 효모발효액을 원심분리하여 얻은 상등액을 여과하여 잔여물을 제거하는 여과단계를 포함한다. 각 단계별로 살펴본다. A method for producing yeast fermentation broth of green tea extract comprises an extraction step of extracting green tea extract by adding an extracting solvent to green tea, a fermentation step of fermenting the green tea extract by adding yeast, an inactivating step of deactivating yeast by heating the yeast fermentation broth, , And a filtration step of filtering the supernatant obtained by centrifuging the yeast fermentation broth to remove the residue. Let's look at each step.
추출물은 시중에 판매하는 추출물을 이용하거나 다양한 방법으로 추출하여 얻을 수 있다. Extracts can be obtained by using commercially available extracts or by extracting them in various ways.
추출물로 천연의 식물로부터 추출된 추출물을 이용한다. 이러한 추출물로 녹차추출물을 이용할 수 있다. 녹차추출물은 천연 화장료 조성물의 재료로 오랫동안 이용되고 있어 인체에 대해 안전성이 입증된 재료이고, 다양한 생리활성을 가지고 있어서 바람직하다. As an extract, extracts extracted from natural plants are used. Green tea extract can be used as such an extract. The green tea extract has been used for a long time as a material of a natural cosmetic composition and is thus proven to be safe for human body and has various physiological activities.
녹차 추출물은 녹차에 추출용매를 가하여 얻는다. 추출용매로 물, 탄소수 1 내지 4의 저급 알코올, 다가 알코올 또는 이들의 혼합물로부터 선택된 적어도 어느 하나를 이용할 수 있다. 탄소수 1 내지 4의 저급 알코올로 메탄올, 에탄올 등을 이용할 수 있고, 다가 알코올로 부틸렌글리콜 및 프로필렌글리콜, 펜틸렌글리콜 등을 이용할 수 있다. 그리고 혼합물로는 물 및 저급 알코올의 혼합물, 물 및 다가 알코올의 혼합물, 저급 알코올 및 다가 알코올의 혼합물, 또는 물 및 저급알코올 및 다가 알코올의 혼합물을 이용할 수 있다. Green tea extract is obtained by adding extraction solvent to green tea. As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.
녹차에 대하여 추출용매를 중량비로 2 내지 40배로 가하여 혼합한 후 10 내지 150℃에서 1 내지 20시간 동안 열수추출, 냉침 또는 온침 추출 방법으로 추출할 수 있다. 또한, 상술한 추출 방법뿐만 아니라 환류냉각추출, 초음파 추출방법 등 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 활성 분획물도 포함할 수 있다. The green tea is mixed with the extraction solvent at a weight ratio of 2 to 40 times, and the mixture is then extracted with hot water, cold or hot extraction at 10 to 150 ° C for 1 to 20 hours. In addition to the above-described extraction method, an active fraction obtained through various purification methods such as a reflux cooling extraction method, an ultrasonic extraction method, and the like may be included.
녹차추출물은 원심분리하여 상등액을 수득한 다음, 상등액을 감압여과하여 고형 잔여물을 제거한다. The green tea extract is centrifuged to obtain a supernatant, and the supernatant is then filtered under reduced pressure to remove the solid residue.
녹차추출물 외에 산자고추출물을 이용할 수 있다. In addition to the green tea extract, Sansago extract can be used.
산자고(Amana edulis)는 백합목 백합과의 여러해살이 풀로서, 양지바른 풀밭에서 자란다. 땅속에 있는 비늘줄기는 달걀 모양 원형으로 길이 3∼4cm 정도이다. 산자고 비늘줄기에는 녹말이 함유되어 있어서 부형제 역할을 할 수 있다. 산자고 비늘줄기는 가을 또는 봄에 캐내 깨끗이 씻은 뒤에 음지에서 말려 자연 건조시킨다. 산자고로부터 추출물을 얻는 추출방법은 상술한 녹차추출물의 추출방법과 동일하게 적용할 수 있다. Amana edulis ) is a perennial plant of lily lilies and grows in sunny grasses. The scales in the ground are oval-shaped and 3-4cm long. The stalks of the Sanjugo scales contain starch, which can act as an excipient. In the autumn or spring, the stalks are dried in the shade and dried naturally. The extraction method for obtaining the extract from Sanjiao can be applied in the same manner as the above-described green tea extract extraction method.
또한, 한약재 추출물이 사용될 수 있다. 한약재 추출물은 기능성을 더욱 높인다. 한약재의 일 예로 고삼, 삼백초, 백선피, 석창포, 측백엽을 동일 중량비로 혼합한 것일 수 있다. 이외에도 천궁, 창출, 홍화, 감초, 당귀, 연교, 인동, 방풍, 형계, 대황, 계지, 도인, 금은화, 황금, 백작약, 황련, 하수오, 조각자, 여정실, 진범 등이 더 추가될 수 있다. 한약재로부터 한약재 추출물을 얻기 위해서 중탕추출할 수 있다. 추출은 공지된 직접가열추출, 중탕추출 등이 가능하지만 한약재들의 유효성분을 효과적으로 추출하기 위해서는 중탕추출에 의해 추출하는 것이 바람직하다. 중탕추출은 중탕기에서 100 내지 120℃로 10 내지 14시간 동안 가열하여 식힌 후 여과기에서 이물질이나 찌꺼기등의 불용성 고형물을 제거하여 얻는다. 한약재추출물은 단독으로 또는 녹차추출물과 혼합하여 이용할 수 있다. 녹차추출물과 혼합하는 경우 녹차추출물 대 한약재추출물을 1: 0.2 내지 0.6의 중량비로 혼합할 수 있다. In addition, herbal medicine extracts may be used. Herbal medicine extracts further enhance functionality. One example of the medicinal herb may be ginseng, saururus chinensis, white radish sprout, seokchonpo mushroom, and hyphae lavender mixed at the same weight ratio. In addition, it can be added to the palace, creation, safflower, licorice, Angelica, allium, persimmon, windshield, serpentine, rhubarb, It can be extracted from hot water to obtain herbal medicine extracts from medicinal herbs. Extraction can be performed by known direct heat extraction, hot water extraction, etc. However, in order to effectively extract active ingredients of herbal medicines, it is preferable to extract by hot water extraction. The hot water extract is obtained by heating in a hot water bath at 100 to 120 ° C for 10 to 14 hours and cooling, and then removing insoluble solids such as foreign substances and debris from the filter. Herbal medicine extracts can be used alone or in combination with green tea extract. When mixed with the green tea extract, the green tea extract and the medicinal plant extract may be mixed at a weight ratio of 1: 0.2 to 0.6.
다음으로, 녹차추출물에 효모를 첨가하여 발효시킨다. 효모로 사카로마이세스 세레비시아(Saccharomyces cerevisiae)를 이용할 수 있다. Next, yeast is added to the green tea extract and fermented. Saccharomyces cerevisiae ( Saccharomyces < RTI ID = 0.0 > cerevisiae ) can be used.
발효시 녹차추출물에 효모추출물(Yeast extract), 맥아추출물(Malt extract), 펩톤(Peptone), 포도당(Dextrose)을 첨가한다. 가령, 녹차추출물 100mL 당 효모추출물 0.3g, 맥아추출물 0.3g, 펩톤 0.5g, 포도당 1g을 첨가할 수 있다. Yeast extract, Malt extract, Peptone, and Dextrose are added to the green tea extract during fermentation. For example, 0.3 g of yeast extract, 0.3 g of malt extract, 0.5 g of peptone, and 1 g of glucose may be added per 100 mL of green tea extract.
녹차추출물에 효모추출물, 맥아추출물, 펩톤, 포도당을 첨가한 후 121℃에 15분간 멸균한 후 효모를 접종한다. 효모를 접종한 후 50 내지 100rpm으로 교반하면서 20 내지 40℃의 온도 조건하에서 pH 5 내지 7을 유지하면서 1 내지 10일간 발효시켜 효모발효액을 수득한다. Yeast extract, malt extract, peptone, and glucose are added to the green tea extract and sterilized at 121 ° C for 15 minutes, followed by inoculation with yeast. Yeast is inoculated and fermented for 1 to 10 days while stirring at 50 to 100 rpm under a temperature condition of 20 to 40 캜 at a pH of 5 to 7 to obtain yeast fermentation broth.
다음으로, 수득한 효모발효액을 열처리하여 효모를 실활시킨다. 이를 위해 효모발효액을 50℃로 1 내지 10분 동안 가열한다. Next, the obtained yeast fermentation broth is heat-treated to inactivate the yeast. For this, the yeast fermentation broth is heated to 50 ° C for 1 to 10 minutes.
다음으로, 실활시킨 효모발효액을 원심분리한 후 상등액을 감압여과하여 고형잔여물을 제거하여 최종적으로 여과된 효모발효액을 제조할 수 있다. Next, the inactivated yeast fermentation broth is centrifuged, and the supernatant is filtered under reduced pressure to remove the solid residue, thereby finally producing the filtered yeast fermentation broth.
효모발효액은 각종 공지의 성분 등을 추가하여 화장료 조성물로 제조할 수 있다. The yeast fermentation broth can be prepared by adding various known components and the like to the cosmetic composition.
본 발명의 화장료 조성물은 조성물 총 중량에 대하여 효모발효액을 0.1 내지 40.0중량%로 함유할 수 있다. 0.1중량% 미만이면 효과가 미미하고, 40.0중량%를 초과하면 발효 시 생기는 특유의 냄새로 인해 사용감을 저해할 수 있다.The cosmetic composition of the present invention may contain the yeast fermentation broth in an amount of 0.1 to 40.0% by weight based on the total weight of the composition. If the content is less than 0.1% by weight, the effect is insignificant. If the content is more than 40.0% by weight, the feeling of use may be deteriorated due to the unique odor generated during fermentation.
본 발명의 화장료 조성물은 당업계에 공지된 방법을 이용하여 각종 공지의 성분 등을 추가하여 화장수, 로션, 크림, 에센스, 팩, 메이크업베이스, 파운데이션, 바디오일, 바디로션, 클렌징오일, 클렌징폼, 헤어오일, 헤어샴푸 및 헤어린스 중에서 선택된 어느 하나의 제형으로 제조될 수 있다.The cosmetic composition of the present invention may be prepared by adding various known components and the like to the cosmetic composition by using a method known in the art to prepare cosmetic lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, Hair oil, hair oil, hair shampoo and hair rinse.
구체적으로 본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 겔, 팩인 경우 공지의 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라가칸트, 셀롤로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.Specifically, when the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel or a pack, known components include animal fibers, vegetable fibers, waxes, paraffin, starch, tragacanth, celluloses derivatives, polyethylene glycols, silicones, bentonites, Silica, talc or zinc oxide may be used.
본 발명의 화장료 조성물의 제형이 파우더인 경우 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used.
본 발명의 화장료 조성물의 제형이 용액 또는 유탁액인 경우 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌글리콜 또는 소르비탄의 비방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or non-acidic esters of sorbitan, and the like.
본 발명의 화장료 조성물의 제형이 현탁액인 경우 물, 에탄올 또는 프로필렌글리콜과 같은 액상 희석제, 에톡실화이소스테아릴알코올, 폴리옥시에틸렌 스르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀롤로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라가칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a suspension, it may be diluted with water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Celluloses, aluminum metahydroxides, bentonites, agar or tragacanth, and the like can be used.
본 발명의 화장료 조성물의 제형이 계면활성제 함유 세정제인 경우 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성유 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a surfactant-containing detergent, it is preferable to use a surfactant-containing detergent such as aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate , Alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils or ethoxylated glycerol fatty acid esters.
이 외에도 본 발명의 화장료 조성물은 통상적인 공지된 성분인 안정화제, 용해화제, 비타민, 안료 및 향료 등을 이용할 수 있으며, 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있고, 사용 대상에 따라 적절하게 선택될 수 있다.In addition, the cosmetic composition of the present invention may be prepared by any of the commonly known formulations such as stabilizers, solubilizers, vitamins, pigments, perfumes, and the like, which are conventionally produced in the art, And can be selected accordingly.
제형의 일 예로 화장수는 에탄올 1.5중량%, 내추럴 리퀴드 1.0중량%, 베타인 3.0중량%, 디엘 판테놀 3.0중량%, 세붐-A 1.0중량%, 알부틴 페이스트 2.0중량%, 아네노신 2.0중량%, 에코검 0.03중량%, 글리세린 4.0중량%, 콜라겐 1.0중량%, 효모 발효액 30.0중량%, 방부제(미량), 정제수(잔량)로 조성될 수 있다.As an example of the formulation, the lotion was prepared by mixing 1.5 wt% of ethanol, 1.0 wt% of natural liquid, 3.0 wt% of betaine, 3.0 wt% of diel panthenol, 1.0 wt% of sebum-A, 2.0 wt% of arbutin paste, 2.0 wt% 0.03% by weight of glycerin, 4.0% by weight of glycerin, 1.0% by weight of collagen, 30.0% by weight of yeast fermentation broth, preservative (small amount) and purified water (balance).
또한, 제형의 일 예로 로션은 카보머 1.0중량%, 디메치콘 2.0중량%, 호호바 오일 5.0중량%, 프로비타민E 0.5중량%, 올리브 유화왁스 1.0중량%, 알부틴 페이스트 5.0중량%, T-믹스처 1.2중량%, 폼믹스처 1.0중량%, 바타인1.0중량%, 세테아릴알코올 1.5중량%, 글리세린 4.0중량%, 플렉스오일 5.0중량%, 알부틴 페이스트 5.0중량%, 효모발효액 30.0중량%, 블랜딩 에센스 오일(미량), 방부제(미량), 정제수(잔량)로 조성될 수 있다.In addition, as an example of the formulation, the lotion may contain 1.0% by weight of carbomer, 2.0% by weight of dimethicone, 5.0% by weight of jojoba oil, 0.5% by weight of provitamin E, 1.0% by weight of olive oil wax, 5.0% by weight of arbutin paste, 1.2 wt%, foam mix 1.0 wt%, batain 1.0 wt%, cetearyl alcohol 1.5 wt%, glycerin 4.0 wt%, flex oil 5.0 wt%, arbutin paste 5.0 wt%, yeast fermentation solution 30.0 wt% Oil (trace amount), preservative (trace amount) and purified water (residual amount).
또한, 제형의 일 예로 크림은 세테아릴알코올 1.5중량%, 글리세릴스테아레이트 1.0중량%, 폴리소르베이트60 1.0중량%, 소르비탄세스퀴올리에이트 0.3중량%, 세틸옥타노에이트 6.0중량%, 스쿠알란 8.0중량%, 아프리코드커넬오일 4.0중량%,디메치콘 2.0중량%, 부틸렌글라이콜 4.0중량%, 글리세린 4.0중량%, 마그네슘알루미늄실리케이트0.4중량%, 효모발효액 30.0중량%, 블랜딩 에센스 오일(미량), 방부제(미량), 정제수(잔량)로 조성될 수 있다.As an example of the formulation, the cream may contain 1.5 wt% of cetearyl alcohol, 1.0 wt% of glyceryl stearate, 1.0 wt% of
또한, 제형의 일 예로 에센스는 에탄올 1.5중량%, 내추럴 리퀴드 1.0중량%, 카보머 1.0중량%, 베타인 3.0중량%, 아데노신 2.0중량%, 알부틴 페이스트 5.0중량%, 히알루론산나트륨수용액(1%) 0.03중량%, 디엘 판테놀 3.0중량%, 글리세린 4.0중량%, 세붐-A 1.0중량%, 효모발효액 30.0중량%, 블랜딩 에센스 오일(미량), 방부제(미량), 정제수(잔량)로 조성될 수 있다.The essence of the formulation is 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 1.0% by weight of carbomer, 3.0% by weight of betaine, 2.0% by weight of adenosine, 5.0% by weight of arbutin paste, 0.03% by weight of diel panthenol, 4.0% by weight of glycerin, 1.0% by weight of sebum-A, 30.0% by weight of yeast fermentation broth, a blending essence oil, a preservative and a purified water.
또한, 제형의 일 예로 파운데이션은 스테아릭애씨드 2.0중량%, 세테아릴알코올 0.5중량%, 글리세릴스테아레이트 1.0중량%, 폴리소르베이트60 0.5중량%, 소르비탄세스퀴올리에이트 0.7중량%, 하이드로제네이티드랩씨드오일 2.0중량%, 해바라기유 5.0중량%, 스쿠알란 6.0중량%, 세틸옥타노에이트 4.0중량%, 사이클로메치콘 2.0중량%, 부틸렌글라이콜 5.0중량%, 글리세린 3.0중량%, 마그네슘알루미늄실리케이트 0.6중량%, 산탄검 0.05중량%, 이산화티탄 10.0중량%, 탈크 3.0중량%, 하이드레이티드페릭옥사이드 1.3중량%, 페릭옥사이드 0.3중량%, 페러스-페릭옥사이드 0.15중량%, 효모발효액 30.0중량%, 방부제(미량), 정제수(잔량)로 조성될 수 있다.As an example of the formulation, a foundation may be prepared by mixing 2.0 wt% of stearic acid, 0.5 wt% of cetearyl alcohol, 1.0 wt% of glyceryl stearate, 0.5 wt% of
또한, 제형의 일 예로 팩은 프로필렌글리콜 0.5중량%, 내추럴 리퀴드 1.0중량%, 폴리비닐알코올 3.0중량%, 에탄올 3.0중량%, 카보머 1.0중량%, 콜라겐 2.0중량%, 에코검 1.0중량%, 펩타이드RF 1.0중량%, 디엘 판테놀 3.0중량%, 글리세린 4.0중량%, 세붐-A 1.0중량%, 아데노신 2.0중량%, 효모발효액 30.0중량%, 블랜딩 에센스 오일(미량), 방부제(미량), 정제수(잔량)로 조성될 수 있다.In addition, as an example of the formulation, the pack may contain 0.5 wt% of propylene glycol, 1.0 wt% of natural liquid, 3.0 wt% of polyvinyl alcohol, 3.0 wt% of ethanol, 1.0 wt% of carbomer, 2.0 wt% of collagen, 1.0 wt% (By weight), 1.0 wt% of RF, 3.0 wt% of diel panthenol, 4.0 wt% of glycerin, 1.0 wt% of sebum-A, 2.0 wt% of adenosine, 30.0 wt% of yeast fermentation broth, ≪ / RTI >
또한, 제형의 일 예로 헤어샴푸는 폴리 쿼터니움 0.5중량%, 내추럴 리퀴드 1.0중량%, 코카미도 프로필베타인3.0중량%, 베타인 3.0중량%, CDA 7.0중량%, LES 25.0중량%, 코코베테인 5.0중량%, 콜라겐 1.0중량%, 디엘 판테놀 3.0중량%, 글리세린 4.0중량%, 세붐 -A 1.0중량%, 아데노신 2.0중량%, 효모발효액 30.0중량%,블랜딩 에센스 오일(미량), 방부제(미량), 정제수(잔량)로 조성될 수 있다.As an example of the formulation, the hair shampoo may contain 0.5% by weight of polyquaternium, 1.0% by weight of natural liquid, 3.0% by weight of cocamidopropyl betaine, 3.0% by weight of betaine, 7.0% by weight of CDA, 25.0% by weight of LES, 5.0 weight% of collagen, 1.0 weight% of collagen, 3.0 weight% of diel panthenol, 4.0 weight% of glycerin, 1.0 weight% of sebum-A, 2.0 weight% of adenosine, 30.0 weight% of yeast fermentation broth, Purified water (remaining amount).
이하, 본 발명의 이해를 돕기 위하여 실시 예를 제시하나, 하기 실시 예는 본 발명을 예시하는 것일 뿐 본 발명의 범위가 하기 실시 예에 한정되는 것은 아니다.EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.
(실시예1)(Example 1)
녹차 40g에 증류수 1L를 가하고 70℃에서 6시간 동안 가열한 후 여과지를 이용하여 고형물을 1차로 걸러내었다. 그리고 6000rpm의 속도로 20분 동안 원심분리하여 상등액을 얻은 다음, 상등액을 감압여과하여 고형 잔여물을 제거한 녹차추출물을 얻었다.1 L of distilled water was added to 40 g of green tea, heated at 70 ° C for 6 hours, and filtered to remove solids. Then, the supernatant was obtained by centrifugation at a speed of 6000 rpm for 20 minutes, and then the supernatant was filtered under reduced pressure to obtain a green tea extract from which the solid residue was removed.
다음으로, 녹차추출물 100mL에 Yeast extract 0.3g, Malt extract 0.3g, Peptone 0.5g, Dextrose 1g을 첨가하여 121℃에 15분간 멸균을 한 후, 무균대에서 효모 Saccaromyces cerevisiae(KCCM 50583)을 2%(v/v)가 되게 접종하였다. 효모 균주를 접종한 녹차추출물은 34℃로 온도를 유지하면서 72시간 동안 80 rpm하에서 발효시켰다. 발효 후 50℃에서 가열하여 효모 균주를 실활시킨 다음, 12,000 rpm으로 20분 동안 2회 원심분리를 한 후 얻어진 상등액을 감압여과하여 고형 잔여물을 완전히 제거하여 효모발효액을 제조하였다. Next, 0.3 g of yeast extract, 0.3 g of malt extract, 0.5 g of peptone, and 1 g of Dextrose were added to 100 mL of green tea extract, and sterilized at 121 ° C for 15 minutes. The yeast Saccaromyces cerevisiae (KCCM 50583) to 2% (v / v). The green tea extract inoculated with the yeast strain was fermented at 80 rpm for 72 hours while maintaining the temperature at 34 ° C. After the fermentation, the yeast strain was inactivated by heating at 50 ° C., followed by centrifugation twice at 12,000 rpm for 20 minutes. The resulting supernatant was filtered under reduced pressure to completely remove the solid residue to prepare yeast fermentation broth.
(실시예 2)(Example 2)
상기 실시예 1의 효모발효액을 첨가하여 아래와 같이 샴푸를 제조하였다.The yeast fermentation broth of Example 1 was added to produce the following shampoo.
효모발효액 42g에 증점제인 폴리쿼터를 0.52g 계량하여 첨가한 후 핫플레이트를 이용하여 70℃로 가열한 상태에서 충분히 겔화가 일어날 때까지 1차로 교반시켰다. 교반 후 음이온성 계면활성제인 LES 40g과 양이온성 계면활성제인 코코베타인 6g, 양쪽성 계면활성제인 CDA 6g을 추가로 혼합하여 2차로 교반하였다. 추가로 보습제로서 글리세린 0.8g, 가용화제로서 올리브 리퀴드 0.4g, 에센스오일 0.1g을 첨가하여 혼합하였다. 0.52 g of a polyquater as a thickening agent was added to 42 g of the yeast fermentation broth, and the mixture was first stirred with heating on a hot plate at 70 캜 until sufficient gelation occurred. After stirring, 40 g of an anionic surfactant LES, 6 g of a cationic surfactant cocobetaine, and 6 g of an amphoteric surfactant, CDA, were further mixed and stirred. Further, 0.8 g of glycerin as a humectant, 0.4 g of olive liquid as a solubilizing agent and 0.1 g of essence oil were added and mixed.
(실시예 3)(Example 3)
상기 실시예 1의 효모발효액을 첨가하여 바디클렌저를 제조하였다. The yeast fermentation broth of Example 1 was added to prepare a body cleanser.
효모발효액 42g에 가용화제인 올리브리퀴드 1.2g을 계량하여 첨가하였다. 교반 후 음이온성 계면활성제인 LES 40g과 양이온성 계면활성제인 코코베타인 6g, 양쪽성 계면활성제인 CDA 6g을 추가로 혼합하여 2차로 교반하였다. 보습 및 기능성 재료로 글리세린 0.8g, 디엘판테놀 0.8g, 베테인 0.7g, 에센스오일 0.2g을 첨가하여 혼합하였다. To 42 g of yeast fermentation broth, 1.2 g of olive liquid as a solubilizing agent was metered in. After stirring, 40 g of an anionic surfactant LES, 6 g of a cationic surfactant cocobetaine, and 6 g of an amphoteric surfactant, CDA, were further mixed and stirred. 0.8 g of glycerin, 0.8 g of diel panthenol, 0.7 g of betaine and 0.2 g of essence oil were added as a moisturizing and functional material and mixed.
(비교예)(Comparative Example)
증류수 100mL에 Yeast extract 0.3g, Malt extract 0.3g, Peptone 0.5g, Dextrose 1g을 첨가하여 121℃에 15분간 멸균을 한 후, 무균대에서 효모 Saccaromyces cerevisiae(KCCM 50583)을 2%(v/v)가 되게 접종하였다. 효모 균주를 접종한 녹차추출물은 34℃로 온도를 유지하면서 72시간 동안 80rpm하에서 발효시켰다. 발효 후 50℃에서 가열하여 효모 균주를 실활시킨 다음, 12,000 rpm으로 20분 동안 2회 원심분리를 한 후 얻어진 상등액을 감압여과하여 고형 잔여물을 완전히 제거하여 효모발효액을 제조하였다. To the 100 mL of distilled water, 0.3 g of yeast extract, 0.3 g of malt extract, 0.5 g of peptone and 1 g of Dextrose were added and sterilized at 121 ° C for 15 minutes. The yeast Saccaromyces cerevisiae (KCCM 50583) Respectively. The green tea extract inoculated with the yeast strain was fermented at 80 rpm for 72 hours while maintaining the temperature at 34 캜. After the fermentation, the yeast strain was inactivated by heating at 50 ° C., followed by centrifugation twice at 12,000 rpm for 20 minutes. The resulting supernatant was filtered under reduced pressure to completely remove the solid residue to prepare yeast fermentation broth.
<제 1실험예: 라디칼 소거능 측정>≪ First Experimental Example: Measurement of radical scavenging ability &
상기 실시예 1에서 얻은 녹차추출물, 실시예 1의 효모발효액, 비교예의 효모발효액에 증류수를 각각 가해 농도별로 희석한 시료를 이용하여 라디칼 소거능을 측정하여 그 결과를 도 1에 나타내었다. The radical scavenging ability of the green tea extract obtained in Example 1, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example were measured by diluting the distilled water by the added concentration, respectively. The results are shown in FIG.
시료 0.3mL와 0.2mM 1,1-diphenyl-2-picryl hydrazyl(DPPH)용액 0.7mL를 혼합하여 암실에서 15분간 방치 후, UV-vis spectrometer를 이용하여 517nm에서 흡광도를 측정하였다. 라디칼 소거능(%)은 다음의 식에 의해 계산되었다. 0.3 ml of the sample and 0.7 ml of 0.2 mM 1,1-diphenyl-2-picryl hydrazyl (DPPH) solution were mixed and left for 15 minutes in a dark room, and the absorbance was measured at 517 nm using a UV-vis spectrometer. The radical scavenging ability (%) was calculated by the following equation.
라디칼소거능(%) = (1-시료첨가구의 흡광도/무첨가구의 흡광도)×100Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of non-additive) x 100
도 1을 참조하면, 비교예의 라디칼소거능이 가장 낮게 나타났다. 그리고 녹차추출물은 비교예보다 라디칼소거능이 더 높은 것으로 나타났다. 그리고 실시예 1의 녹차추출물의 효모발효액은 녹차추출물에 비해 라디칼소거능이 크게 향상된 것으로 확인되었다. 이로부터 녹차추출물을 효모로 발효시키면 녹차추출물의 항산화능을 크게 증대시킬 수 있음을 알 수 있었다. 따라서 녹차추출물의 효모발효액은 화장료 조성물의 재료로 이용하는 경우 화장료 조성물의 항산화 효과가 우수할 것으로 기대된다. Referring to FIG. 1, the radical scavenging ability of the comparative example was the lowest. And green tea extract showed higher radical scavenging ability than the comparative example. It was also found that the yeast fermentation broth of the green tea extract of Example 1 was greatly improved in radical scavenging ability compared to the green tea extract. From this, it was found that the fermentation of green tea extract with yeast could significantly enhance the antioxidant activity of green tea extract. Therefore, it is expected that the yeast fermentation broth of green tea extract is excellent in the antioxidative effect of the cosmetic composition when it is used as a material for a cosmetic composition.
<제 2실험예: 총 폴리페놀 함량 측정>≪ Second Experimental Example: Determination of total polyphenol content >
실시예 1에서 얻은 녹차추출물, 실시예 1의 효모발효액, 비교예의 효모발효액의 시료와 Folins-denis reagent를 1:1로 혼합 후, 10% Na2CO3를 첨가하여 일정시간 경과 후 700nm에서 흡광도를 측정하였다. Tannic acid를 기준으로 총 폴리페놀 함량을 측정하여 도 2에 나타내었다. The green tea extract obtained in Example 1, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example were mixed with Folins-denis reagent at a ratio of 1: 1, 10% Na 2 CO 3 was added, Were measured. The total polyphenol content was measured based on tannic acid and is shown in FIG.
도 2를 참조하면, 비교예의 총 폴리페놀 함량이 가장 낮게 나타났다. 그리고 녹차추출물은 비교예보다 총 폴리페놀 함량이 더 높은 것으로 나타났다. 그리고 실시예 1의 녹차추출물의 효모발효액은 녹차추출물에 비해 총 폴리페놀 함량이 크게 향상된 것으로 확인되었다. 이러한 결과는 상기의 라디칼 소거능 측정 결과와 일치한다. Referring to Figure 2, the total polyphenol content of the comparative example was the lowest. And green tea extract showed higher total polyphenol content than the comparative example. The yeast fermentation broth of green tea extract of Example 1 was found to have a greater total polyphenol content than green tea extract. These results are consistent with the results of the above-mentioned radical scavenging ability measurement.
<제 3실험예: 효모 발효 여과액의 총 플라보노이드 함량>≪ Third Experimental Example: Total flavonoid content of yeast fermentation filtrate >
실시예 1에서 얻은 녹차추출물, 실시예 1의 효모발효액, 비교예의 효모발효액의 시료를 1N NaOH 그리고 Diethylene glycol을 일정비율로 혼합하여 30분간 방치 후 420nm에서 흡광도를 측정하였다. Quercetin을 기준물질로 하여 총 플라보노이드 함량을 측정하여 도 3에 나타내었다. The green tea extract obtained in Example 1, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example were mixed at a constant ratio of 1N NaOH and diethylene glycol, and left for 30 minutes, and the absorbance was measured at 420 nm. The total flavonoid content was measured using quercetin as a reference material and is shown in FIG.
도 3을 참조하면, 비교예의 총 플라보노이드 함량이 가장 낮게 나타났다. 그리고 녹차추출물은 비교예보다 총 플라보노이드 함량이 더 높은 것으로 나타났다. 그리고 실시예 1의 녹차추출물의 효모발효액은 녹차추출물에 비해 총 플라보노이드 함량이 크게 향상된 것으로 확인되었다. 이러한 결과는 상기의 라디칼 소거능 측정 결과 및 총 폴리페놀 함량측정 결과와 일치한다. Referring to FIG. 3, the total flavonoid content of the comparative example was the lowest. And green tea extract showed higher total flavonoid content than the comparative example. The yeast fermentation broth of the green tea extract of Example 1 was found to have a significantly higher total flavonoid content than the green tea extract. These results are consistent with the measurement results of the radical scavenging ability and the total polyphenol content.
<제 4실험예: 화장료 조성물의 자극성 확인>≪ Fourth Experimental Example: Confirmation of irritancy of cosmetic composition >
실시예 2의 샴푸 및 실시예 3의 바디클렌저의 피부 자극 정도를 알아보기 위해 (주)더마프로 피부과학연구소에 피부 테스트를 의뢰하였다. 피부테스트 실험결과 보고서의 요약문을 도 4에 첨부하였다. To examine the degree of skin irritation of the shampoo of Example 2 and the body cleanser of Example 3, a skin test was commissioned by the Dermar Pro skin Science Institute. A summary of the skin test results report is attached in FIG.
총 31명이 본 시험의 전 과정에 참여하였다. 피험자들의 평균 연령은 37.6±8.5세였으며, 최고 연령자는 50세, 최저 연령자는 20세였다. A total of 31 participants participated in this study. The mean age of the subjects was 37.6 ± 8.5 years. The highest age group was 50 years old and the lowest age group was 20 years old.
시험 부위는 70% ethanol로 세척한 뒤 건조시켰다. 그리고 시험 물질은 wash-off 제품의 특성상 증류수에 1%로 희석하여 적용하였다. 시험물질 16㎕를 Finn chamber® 내에 적하시킨 후 시험부위인 등 부위에 얹고, micropore tape으로 고정시켰다. 첩포는 48시간 동안하며, 첩포를 제거한 후에는 skin marker로 시험 부위를 표시하고 30분, 24시간 후에 각 시험 부위를 관찰하였다. The test area was washed with 70% ethanol and then dried. The test substance was diluted to 1% in distilled water due to the nature of the wash-off product. 16 μl of the test substance was dropped into the Finn chamber, placed on the back of the test site, and fixed with a micropore tape. After removing the patches, the test area was marked with a skin marker, and each test site was observed after 30 minutes and 24 hours.
관찰은 첩포제거 후 30분, 24시간 경과시 이루어지며 피부반응은 Frosch& Kligman1)법과 Personal Care Product Council(PCPC) guideline을 반영한 기준에 따라 평가하였다. Observations were made 30 minutes and 24 hours after removal of the patch, and skin reactions were evaluated according to the criteria reflecting the Frosch & Kligman1 method and the Personal Care Product Council (PCPC) guideline.
시험결과, 2종의 시험물질에서 각각 1명의 피험자가 1+ grade 피부반응을 보이는 것으로 나타났다. 따라서 2종의 시험물질은 인체피부 일차자극 측면에서 저자극 범주의 물질로 확인되었다. As a result of the test, 1 subject had a 1+ grade skin reaction in each of the two test substances. Therefore, the two test substances were identified as low-irritant substances in terms of primary skin irritation.
이상, 본 발명은 일 실시 예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 실시 예가 가능하다는 점을 이해할 것이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation.
Claims (6)
상기 녹차추출물에 효모를 첨가하여 발효시키는 발효단계와;
상기 발효단계에서 수득한 효모발효액을 열처리하여 효모를 실활시키는 실활단계와;
상기 실활단계 후 상기 효모발효액을 원심분리하여 얻은 상등액을 여과하여 잔여물을 제거하는 여과단계;를 포함하는 것을 특징으로 하는 녹차추출물의 효모발효액 제조방법. An extracting step of extracting a green tea extract by adding an extracting solvent to the green tea;
A fermentation step of adding yeast to the green tea extract to ferment;
An inactivating step of inactivating the yeast by heat-treating the yeast fermentation broth obtained in the fermentation step;
And filtering the supernatant obtained by centrifuging the yeast fermentation broth after the inactivation step to remove residues of the yeast fermentation broth.
피부에 저자극성인 것을 특징으로 하는 효모 발효를 이용한 화장료 조성물. A lotion, a cream, an essence, a pack, a make-up base, a foundation, a body oil, a body lotion, a cleansing oil, a cosmetic lotion, and the like, containing 0.1 to 40 wt.% Of a yeast fermentation broth which fermented green tea extract, A cleansing foam, a hair oil, a hair shampoo and a hair rinse,
A cosmetic composition using yeast fermentation characterized by being hypoallergenic to the skin.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109694886A (en) * | 2017-10-19 | 2019-04-30 | 安琪酵母股份有限公司 | A kind of green tea fermentation filtrate and its preparation method and application |
| KR20210104456A (en) * | 2020-02-17 | 2021-08-25 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-oxidant and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
| KR102292975B1 (en) * | 2020-02-17 | 2021-08-26 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-wrinkling and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
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| CN113925814A (en) * | 2021-11-04 | 2022-01-14 | 安蒂加(上海)生物科技有限公司 | Moisturizing essence containing yeast green tea fermentation product and preparation method thereof |
| CN116509766A (en) * | 2023-03-16 | 2023-08-01 | 东莞巨微新材料科技有限公司 | Green tea symbiotic fermentation liquor with antioxidant, oil control and anti-wrinkle effects and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109694886A (en) * | 2017-10-19 | 2019-04-30 | 安琪酵母股份有限公司 | A kind of green tea fermentation filtrate and its preparation method and application |
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| KR20210104456A (en) * | 2020-02-17 | 2021-08-25 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-oxidant and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
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| KR102298533B1 (en) * | 2020-10-30 | 2021-09-07 | 주식회사 아미코스메틱 | Antianging cosmetic composition comprising green tea fermented extract fermented by inoculating lactococcus lactis strain |
| CN113908093A (en) * | 2021-11-04 | 2022-01-11 | 安蒂加(上海)生物科技有限公司 | A kind of moisturizing essence milk containing yeast green tea fermentation product and preparation method thereof |
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| KR20230148576A (en) * | 2022-04-18 | 2023-10-25 | 코스맥스 주식회사 | Cosmetic composition comprising fermented green tea |
| CN116509766A (en) * | 2023-03-16 | 2023-08-01 | 东莞巨微新材料科技有限公司 | Green tea symbiotic fermentation liquor with antioxidant, oil control and anti-wrinkle effects and preparation method and application thereof |
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