KR20190065981A - Anti-bacterial fabric product comprising anti-bacterial composition with effect of viability of lactic acid bacteria and anti-bacterial activity and manufacturing method thereof - Google Patents

Abstract

The present invention relates to an antibacterial fiber product including an antibacterial composition extracted and processed from a plant and a method for producing the antibacterial fiber product. The antibacterial fiber product is useful for protecting skin lactic acid bacteria which are beneficial bacteria of human body, It is not only harmless but also has excellent antimicrobial and anti-mold performance, and has antimicrobial effect against super bacteria MRSA.

Description

[0001] The present invention relates to an antibacterial fiber product comprising an antibacterial composition excellent in viability and antibacterial activity against lactic acid bacteria, and an antibacterial fiber product comprising the antibacterial composition,

The present invention relates to an antibacterial fiber product including an antibacterial composition extracted and processed from a plant and a method for producing the antibacterial fiber product. More particularly, the present invention relates to a antibacterial fiber product which is useful for protecting skin lactic acid bacteria, And an antimicrobial composition having antimicrobial activity against MRSA which is a super bacterium, and a method for producing the same.

Preservatives having the function of preventing the growth and proliferation of microorganisms, that is, bacteria, are added to products such as foods, cosmetics, and clothing widely used in daily life to prevent changes in product properties and to reduce human toxicity by microorganisms have.

In the case of cosmetic products, it is difficult to avoid contamination by microorganisms generated during the manufacturing process, contamination by skin contact of fingers during use, or contamination by other substances such as water introduced from the outside during use. Therefore, Preservatives are formulated for the purpose of inhibiting the growth of microorganisms and preventing changes in the physical properties of the composition during use and preventing contamination. If preservatives are not formulated, aseptic manufacturing processes are required to ensure buoyancy, or complex means are required, such as limiting the amount of content or duration of use, or making containers specially, and in such cases, lack of economic versatility The possibility increases.

On the other hand, since textile products such as clothes directly contact the skin, sweat and sebum secreted from the human body are absorbed. In this area, an environment in which harmful bacteria remaining on the skin or harmful bacteria introduced from the outside can easily grow Therefore, wearing the clothes for a long period of time or wearing them over various days causes not only various diseases to the human body but also problems such as staining and coloring, and further, the strength of the fibers is lowered to reduce the durability of the clothes. And a technique capable of imparting antimicrobial properties to textile products including clothing.

In general, in order to impart antibacterial activity to various living products such as cosmetics and clothing, a metal such as silver, or an inorganic particle containing it, a p-oxybenzoic acid ester which is excellent in antimicrobial activity and is well known as parabens, imidazolidinyl urea , Phenoxyethanol, methylchloroisothiazolinone, methylisothiazolinone, chlorophenesin and the like are used. In particular, fiber products include at least one of two types of dissolution type, in which the antimicrobial agent gradually becomes liberated from the fiber as time goes by, and the cost disruption that stops the activity of the microbial cell and destroys the cell wall when the antibacterial agent covalently bonded with the fiber comes into contact with the bacteria Preservatives or antimicrobial agents with an antimicrobial mechanism are used.

As described above, the chemical synthetic materials used as preservatives are excellent as preservatives, but they have problems of skin toxicity, skin irritation, irritation, skin allergy, potential as an environmental hormone and induce resistant bacteria. Recently, non-paraffinic preservatives 1,2 hexane-diol, or xylitol, but if the product contains very small amounts of preservatives, it will be classified as a hazardous product Recognition and excessive use of antimicrobial agents are prohibited, and the use of preservatives is being refrained.

In addition, frequent use of such preservatives or antibiotics results in strains resistant to antiseptics or antibiotics, which require more powerful antibiotics, which, once repeated, will eventually lead to superb bacteria that can resist any potent antibiotic All.

Methicillin-Resistant Staphylococcus aureus (MRSA) is a methicillin-resistant Staphylococcus aureus (Staphylococcus aureus). It is found in a large general hospital that uses antibiotics as a major cause of hospital infection. It is used in the air, the body parts of doctors and nurses, Hospital blanket, tube and other medical equipment attached to live for about three hours and is known to have a strong viability and fertility.

However, the non-paraffinic antimicrobial agent does not exhibit the killing effect against super bacteria such as MRSA, but rather proliferates, resulting in a problem that the antibacterial agent is ineffective as an antibacterial means.

Therefore, many people feel the necessity of a natural antimicrobial agent which is low in human toxicity but excellent in buoyancy, and studies on natural antimicrobial agents excellent in safety and economy continue. However, practically, the price is high and the antibacterial effect is weak It is frequently encountered that the antimicrobial activity is not exhibited, and when it is used in a high dose, safety is deteriorated due to discoloration, deodorization, and skin irritation.

In addition, even when an antimicrobial composition having an antimicrobial effect is applied to a textile product, the same or similar antimicrobial effect is not exhibited, or the component exhibiting an antimicrobial effect is easily separated from the textile product, It is necessary to develop a fiber product including an antimicrobial composition which is excellent in fiber adsorption property and excellent in flotation resistance against microorganisms including super bacteria such as MRSA.

Registration No. 10-1393008 (Registered on March 14, 2014)

The present invention provides a fiber product including a natural antimicrobial composition which is harmless to the human body while having an excellent antibacterial ability and anti-mold ability against microorganisms including super bacteria such as MRSA, and a method for producing the same.

The present invention also provides a fiber product comprising a natural antimicrobial composition which is excellent in deodorization performance, can protect skin lactic acid bacteria, which are beneficial bacteria of the human body, and has improved adhesion to a fiber product without using a separate binder, and a method for producing the same.

In order to achieve the above object, one embodiment of the present invention relates to an antibacterial fiber product treated with an antibacterial treatment liquid, wherein the antibacterial treatment liquid comprises an antimicrobial composition extracted from a plant and water, The composition comprises minerals and ions.

The mineral is calcium (Ca), magnesium (Mg), potassium (K), phosphorous (P), includes at least any one or more of silicon (Si), the ions, chloride ions (Cl ^-), sulfate ion (SO ₄ ^- ) and ammonium ions (NH ₄ ⁺ ).

The antibacterial fiber product may be an antibacterial treatment using the antibacterial treatment liquid in the state of a yarn or fabric.

The antimicrobial fiber product may be at least one selected from the group consisting of a wipe, a wallpaper, a mask, a filter, a medical article, a sanitary article, a clothing, a bedding, a shoe, an automobile seat, a leather product,

Another embodiment of the present invention relates to a method for producing an antibacterial fiber product that imparts antimicrobial properties to a fabric by treating the antibacterial treatment solution containing water and an antimicrobial composition extracted from plants with a fabric.

The antimicrobial composition, calcium (Ca), magnesium (Mg), potassium (K), phosphorous (P), includes at least one or more minerals of silicon (Si), sulfate (SO ₄ ^2-), chloride ion ( Cl ^- ), and ammonium ion (NH ₄ ⁺ ).

One embodiment of the method for manufacturing an antibacterial fiber product includes mixing water and the antibacterial composition to prepare an antibacterial treatment liquid, and allowing to stand or agitate for a predetermined time; Adding a raw material to the antibacterial treatment liquid to perform antibacterial treatment; And applying the dye and heating to fix the dye to the fabric.

Another embodiment of the method for producing an antimicrobial fiber product comprises the steps of: putting a fabric into water; Adding the antimicrobial composition to the water, and allowing to stand or agitate for a predetermined time; And applying the dye and heating to fix the dye to the fabric.

Another embodiment of the method for manufacturing an antibacterial fiber product includes the steps of depositing a fabric on a fabric by immersing a fabric in a first water containing a dye and heating the fabric for a predetermined time; Removing the fabric from the first water containing the dye, washing with water and drying to obtain a dyed fabric; And a step of putting the dyed fabric into a second water containing the antimicrobial composition and the additive to perform antibacterial and padding treatment.

The antimicrobial fiber product comprising the antimicrobial composition of the present invention can help protect skin lactic acid bacteria, which is a beneficial organism of the human body, has excellent deodorizing power, is harmless to human body and has excellent antimicrobial and antifungal performance, It has an excellent antibacterial power.

In addition, the antibacterial fiber product including the antimicrobial composition of the present invention has an effect of protecting the skin by promoting keratinocyte migration of keratinocytes.

1 to 3 are test results of the antibacterial activity test using the stock solution of the antimicrobial composition of the present invention.
4 to 5 are test results of anti-fungal activity test using the stock solution of the antimicrobial composition of the present invention.
6 to 14 are deodorization test report sheets using the stock solution of the antimicrobial composition of the present invention.
15 to 18 are test results of the antibacterial activity of the wet tissue treated with the antimicrobial composition of the present invention.
19 to 29 are test results of antimicrobial activity of a fabric subjected to antibacterial treatment with the antimicrobial composition of the present invention.
30 to 33 are test reports of deodorant force of a fabric subjected to antibacterial treatment with the antimicrobial composition of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings. Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. As well as the fact that

Throughout this specification, when an element is referred to as "including" an element, it is understood that it may include other elements as well, without departing from the other elements unless specifically stated otherwise.

In each step, the identification code is used for convenience of explanation, and the identification code does not describe the order of the steps, and each step may be performed differently from the stated order unless clearly specified in the context. have. That is, each of the steps may be performed in the same order as described, or may be performed substantially concurrently or in the reverse order.

The term "extract" in this specification is meant to encompass all of the natural materials obtained by extracting components from plants, regardless of the extraction method, extraction solvent, extracted components or extract form, and the minerals constitute the human body Refers to all elements other than oxygen, carbon, hydrogen, and nitrogen, which are the main constituents of organic matter among the elements required for physiological activities such as growth and maintenance.

An antimicrobial treatment product treated with an antimicrobial treatment liquid comprises an antimicrobial composition and water extracted from a plant and having excellent antimicrobial and lactic acid bacterial viability, So that the fiber product is impregnated or coated with an antimicrobial composition containing specific minerals and ions.

Here, the fiber product is not particularly limited as long as it is made of fiber or a product containing fibers, and can be used in various fields such as wipes, wallpaper, mask, filter, medical supplies, sanitary goods, clothes, bedding, shoes, Leisure and travel goods.

Such an antibacterial fiber product has an advantage of being excellent in antimicrobial activity, antifungal ability, survival ability of lactic acid bacteria and deodorizing ability and remarkably lowering human toxicity than conventional chemical antibacterial agent because it contains a vegetable antibacterial composition to be described later.

When the antimicrobial composition is impregnated or coated in a fiber product, it can be applied to a fiber in a state of a yarn or a fabric. In the case of applying the antimicrobial composition to a fiber in a yarn state, that is, a fabric made from a yarn having an antibacterial performance by impregnating or coating an antimicrobial composition and an antibacterial fiber product produced using the same, the durability of the antibacterial fiber product is deteriorated Therefore, it is more preferable that the antimicrobial composition is applied to a fiber product in a fabric state, but it can also be applied to a fiber in a yarn state.

The antimicrobial composition contained in the antimicrobial fiber product is an antibacterial aqueous solution extracted from a plant. The antimicrobial composition has excellent antimicrobial activity, anti-mold ability and deodorizing power, and is also excellent in viability of skin lactic acid bacteria. Such the antimicrobial composition may be an antimicrobial composition comprising a commercially available product ^® ESN of bio ESN to the main composition.

In addition, the antimicrobial composition promotes keratinocyte migration of the keratinocyte, thereby rapidly repairing damage to the skin surface, thereby protecting the skin. More specifically, the movement of the keratinocyte is rapidly promoted within 4 hours based on the time when the antimicrobial composition comes into contact with the skin, and the skin is effectively protected by almost completely moving the keratinocyte within 8 hours.

Since the antimicrobial composition is particularly excellent in viability of lactic acid bacteria and proliferates in the antimicrobial composition rather than in the antimicrobial composition, when the antimicrobial composition is applied to a cosmetic product or a skin preparation for skin contact with the skin, it increases the number of skin lactic acid bacteria, It is possible to obtain an effect of increasing skin elasticity and improving skin tone.

When antimicrobial treatment is applied to a fiber product using the above-mentioned antimicrobial composition, the survival rate of the lactic acid bacteria is excellent in the antibacterial fiber product, but the proliferation effect of the lactic acid bacteria may not be exhibited.

However, in case of applying natural antimicrobial agent or chemical synthetic antimicrobial agent to fibers, all the lactic acid bacteria are killed together with harmful bacteria, while the survival rate of lactic acid bacteria is remarkably improved in antimicrobial fiber products treated with the antimicrobial composition of the present invention, It is possible to obtain a remarkable improvement in the problems of skin allergic reaction and the like which appear when an antibacterial fiber product to which another natural antibacterial agent or a chemical synthetic antibacterial agent is applied is used.

The antimicrobial composition is a colorless and odorless aqueous solution containing a non-mineral natural minerals component and a complex inorganic salt. The extract extracted from plants can be used as it is, but it is preferable to use an ultrafiltration membrane having a constant molecular weight cut- Separation or separation by various chromatographies (produced for separation by size, charge, hydrophobicity or affinity), fermentation by using natural state or various microorganisms, reprocessing by various purification methods and active fractions, .

The antimicrobial composition may contain minerals and ions. Specifically, the antimicrobial composition may contain at least one of minerals and at least one of calcium (Ca), magnesium (Mg), potassium (K), phosphorus (P) (SO ₄ ^2- ), chloride ion (Cl ^- ), and ammonium ion (NH ₄ ⁺ ). Preferably, the calcium (Ca), magnesium (Mg), potassium (K), phosphorous (P), silicon (Si), sulfate (SO ₄ ^2-), chloride ion (Cl ^-) and ammonium ion (NH ₄ ⁺ And may be inorganic salts containing the minerals and ions, or may be a mixture of the minerals and ions in the aqueous solution.

The total content of minerals and ions contained in 1 L of the antimicrobial composition may be 15 to 92 mg, and the main components are calcium (Ca) 3,100 to 23,200 占 퐂 / L, magnesium (Mg) 1,107 to 7,320 占 퐂, potassium ) 55 ~ 4,820 ㎍, phosphorus (P) may include a 150 ~ 820 ㎍, silicon (Si) 350 ~ 21,250 ㎍ and sulfate ion ^{_{(SO 4 2-) 5,433 ~ 16,200}} ㎍.

When the antimicrobial composition is applied to a textile product, a cosmetic product, a detergent, a detergent, a soap, a ceramic product, etc., by containing the minerals and ions, it is possible to suppress the growth of microorganisms in the product, And the effect of proliferating lactic acid bacteria can also be exhibited, and it has the effect of eliminating odors generated in daily life and odors generated in chemical products.

Specifically, the antimicrobial composition according to one embodiment of the present invention has antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pneumococcus, Candida, Pseudomonas and Salmonella, etc., and has antibacterial activity against Aspergillus niger, Penicillium pinophilum Penicillium pinophilum, Chaetomium globosum, Aspergillus flavus and Aspergillus versicolor, which have been found to have antimicrobial activity against bacteria and fungi And the bacterium and mold exhibiting antibacterial and antifungal properties are not limited to the above-mentioned species.

In addition, since the antimicrobial composition has antibacterial activity against MRSA (Methicillin-Resistant staphylococcus aureus), which is a super-bacterium, it has a broader range of antibacterial activity than general antibacterial agents.

On the other hand, conventional antimicrobial-treated fiber products have antibacterial and antifungal effects at the initial stage of use of the fiber product because the bonding strength between the fibers constituting the fiber product and the antibacterial agent exhibiting antibacterial and antifungal effects is weak. However, The antibacterial agent is detached from the fibers after washing, and the antibacterial effect is deteriorated for a long period of time. Therefore, a binder is used together to increase the binding effect of the antibacterial agent on the fibers.

Generally, as such a binder, a resin selected from the group consisting of an acrylic resin, a polyurethane resin, a copolymer thereof, and a mixture thereof is used for physically bonding an antibacterial agent to a fiber product, and in order to further improve the binding force, Mixtures of active agents may be used.

However, since the resin binder described above has a problem of deteriorating the durability, tactile feeling, wrinkle resistance and the like inherent in the fiber, it is not used if it is not necessary to impart a special function such as an improvement in bonding force of the antibacterial agent desirable.

In the present invention, since the antimicrobial treatment liquid used in the antibacterial treatment of fiber products has a high binding force to the fiber product without using a separate binder, the problem caused by the binder described above can be solved by not using a binder.

Another embodiment of the present invention relates to a method for producing an antibacterial fiber product, and more particularly, to a method for producing an antibacterial fiber product by treating a fabric using an antibacterial solution containing the antibacterial composition described above.

Generally, the fiber processing is performed by immersing a fabric for a predetermined time in water containing a processing agent such as a dye, an antistatic agent, a water repellent agent, an anti-wrinkle agent, an ultraviolet shielding agent, a chemical synthetic antimicrobial agent, and the like, followed by heat treatment, washing with water, drying, In the case where dyeing processing using dyes and processing using other processing agents are performed at the same time, dyeing with dyes is not properly performed, and it is not only difficult to produce a fabric having a color or pattern desired by the user, Other effects such as antistatic effect and water repellency are deteriorated. In general, after the dyeing processing is performed, a processing treatment using an antistatic agent, a water repellent agent, an anti-wrinkle agent, a UV- .

However, the antimicrobial composition according to an embodiment of the present invention does not deteriorate the fastness to dyeing and antimicrobial activity, antifungal property, and deodorization property due to the antimicrobial composition even when it is mixed with water, that is, water, Antibacterial processing using an antimicrobial composition can be applied simultaneously in the post-processing step as well as the dyeing step.

For example, the antibacterial processing step may be applied in the dyeing step. Specifically, the antibacterial processing solution containing water and the antimicrobial composition is prepared, and after stood or stirred for about 15 to 30 minutes, To allow the antimicrobial treatment liquid to sufficiently penetrate into the fabric, then the dyestuff is put into the fabric, the fabric is heated to a predetermined temperature, and the dyestuff is fixed to the fabric by being kept for a predetermined period of time.

At this time, as the antibacterial treatment liquid, it is preferable to use the antibacterial treatment liquid containing the antibacterial composition in an amount of about 0.1 to 10 vol%.

Thereafter, a cleaning step for removing the unfixed dye, a drying step for drying the fabric, and a heat treatment step for stabilizing the shape of the fabric can be further performed.

Specifically, the dyeing step after the dye is added can be carried out in the following manner.

First, a heating step is carried out at a temperature raising rate of 0.8 to 3.0 占 폚 / min such that the temperature is 50 to 150 占 폚, and then, when the target temperature is reached, the heating step is maintained for about 30 to 80 minutes. At this time, in the case of the cotton fabric, the target temperature is preferably 50 to 80 ° C, the heating rate is 0.8 to 2.0 ° C / min, the holding time is 40 to 80 minutes, 3.0 DEG C / min, and a holding time of 30 to 60 minutes.

If the dyeing temperature is less than the above-mentioned temperature, or the holding time is less than the above-mentioned time range, the fixing of the dye is not sufficiently carried out, and if the dyeing temperature, the heating rate and the holding time exceed the above- Therefore, it is preferable that the heating step is performed under the above-described conditions.

Next, when the final temperature exceeds 60 캜, a cooling step of cooling at a cooling rate of 1 to 2.5 캜 / min and cooling the final temperature to 60 캜 is performed. At this time, if the final temperature in the heating step is 60 DEG C or less, the present cooling step is omitted.

Thereafter, in order to remove the unfixed dye, the temperature is raised to 70 to 90 DEG C, and then the washing step is performed for 8 to 30 minutes.

In the cleaning step, it is preferable to selectively add an alkali material, a reducing agent, a soaping agent, and the like depending on the kind of the dye and the kind of the fabric to perform the cleaning operation. For example, NaOH may be used as the alkali substance, Na ₂ S ₂ O ₄ may be used as the reducing agent, and TC 300S of Nikka Korea may be used as the soaping agent, but the present invention is not limited thereto.

In the above example, when the volume ratio (vol%) of the antimicrobial composition to the total volume of the antimicrobial treatment liquid is less than 0.1, it is difficult to impart the antimicrobial property to the fabric due to a small amount of the antimicrobial composition. It is not economically disadvantageous because additional antimicrobial properties are not imparted. In addition, since the antimicrobial composition is contained in an excessive amount, the dyeing effect by the dye may be lowered.

Another example of the antibacterial processing step is to add the antimicrobial composition so that the volume ratio of the antimicrobial composition in the total antimicrobial treatment solution becomes 0.1 to 10 vol% The mixture is stirred for 15 to 30 minutes, and then the dye is added. After the dye is added, the heating, cooling, and washing steps may be performed in the same manner as in the above example.

On the other hand, the antibacterial processing step may be carried out not in the dyeing step but also in the post-processing step (padding step). In this case, the antibacterial processing step includes the steps of dying the dyed fabric with water, the antibacterial composition and other processing agent And removing the fabric after a predetermined period of time and drying the fabric.

In this case, the dyeing step may be a dyeing step which is usually performed in the art, for example, by immersing a cloth in water containing the dye and heating it for a predetermined time to fix the dye on the cloth , And removing the fabric from the water containing the dye and washing with water.

The other processing agent may include at least one selected from the group consisting of an antistatic agent, a water repellent agent, an anti-wrinkle agent, and an ultraviolet screening agent. However, the present invention is not limited thereto. Since the antimicrobial composition according to the embodiment is included, no separate chemically synthesized antimicrobial agent is used as the other processing agent.

Hereinafter, an embodiment of the present invention will be described. However, the scope of the present invention is not limited to the following preferred embodiments, and a person skilled in the art can carry out various modifications of the contents described in the present invention within the scope of the present invention.

[ Manufacturing example ]

Preparation of antimicrobial composition

The plants collected from nature were dried, immersed in a solvent, heated at a high temperature for a certain period of time, removed from solids, filtered and purified to prepare an antimicrobial composition.

Preparation of antimicrobial wet tissue containing antimicrobial composition

5 mL of the antimicrobial composition prepared above was mixed with 500 mL of purified water to prepare an antimicrobial treatment liquid. Then, a 5 × 5 cm nonwoven fabric was immersed for 20 minutes to prepare an antimicrobial wet tissue.

Preparation of Antibacterial Fabrics Containing Antimicrobial Compositions

An antimicrobial treatment liquid was prepared by mixing 5 mL of the antimicrobial composition prepared before 500 mL of water. After 20 minutes, the fabric of a predetermined size was immersed in the antimicrobial treatment liquid, the dye was added, the temperature was raised, An antibacterial fabric was prepared.

[ Experimental Example  One]

Experiment of antibacterial activity of antimicrobial composition

10 mL of the antimicrobial composition prepared in the above Preparation Example was placed in an Erlenmeyer flask, sterilized at 121 ° C for 15 minutes using a sterilizer, and then 90 mL of physiological saline (NaCl 8.5%) and 1 mL of the cultured test strain were added. Thereafter, the cells were shake cultured at 37.0 ± 0.2 ° C in a 31.7 ± 0.2% R.H environment for 24 hours, and then a certain amount of the cells was plated on an agar medium and then the number of bacteria was measured.

At this time, the test strain was cultured in Nutrient liquid medium, and MRSA (Staphylococcus aureusubsp. Aureus ATCC 33591), which is a superbacterium, was used as a test strain.

As Comparative Example 1, 100 mL of physiological saline was used to culture the test strain by the same method as described above, and then the number of bacteria was measured.

Test Items Initial concentration (CFU / mL) After 24 hours concentration (CFU / mL) Bacterial reduction rate (%) MRSA Example 1 1.7 x 10 ⁴ <10 99.9 Comparative Example 1 1.7 x 10 ⁴ 5.5 × 10 ⁵ -

As shown in Table 1 and FIGS. 1 to 3, in the case of the test group containing the antimicrobial composition of the present invention, it was confirmed that the bacterial reduction rate was 99.9% due to the death of most of the bacteria after 24 hours of inoculation with the superbacterial MRSA I could.

On the other hand, in Comparative Example 1 which did not include the antimicrobial composition of the present invention, the concentration of MRSA increased after 24 hours, indicating that the antimicrobial composition of the present invention had antimicrobial activity against MRSA.

Antimicrobial Antifungal  Performance experiment

10 mL of the antimicrobial composition prepared in the above Preparation Example was inoculated into 90 mL of the test strain and the mixture was allowed to stand for 24 hours and cultured for 4 weeks to determine the growth of the fungus in the environment of 29.0 ± 0.2 ° C. and 99.0 ± 1.0% RH .

The test strains were selected from the group consisting of black mold Aspergillus niger ATCC 9642, Penicillium pinophilum ATCC 11797, Chaetomium globosum ATCC 6205 and Aspergillus flavus ATCC 9643 ) And Aspergillus versicolor ATCC 11730 were mixed and used.

Referring to FIG. 4 and FIG. 5, when the antimicrobial composition was inoculated with the test strain mixed spore solution and cultured for 4 weeks, the mold was not grown.

That is, it was confirmed that the antimicrobial composition of the present invention had antifungal ability against the test strain.

Experiment on the viability of lactic acid bacteria in antimicrobial composition

After the lactic acid bacteria were cultured in a liquid medium, the cultured lactic acid bacteria were diluted to an initial bacterial concentration of 1.5 x 10 &lt; ⁵ &gt; CFU / mL, and 0.1 mL of the lactic acid bacterial dilution was added to 10 mL of the antimicrobial composition prepared in the above Preparation Example. After incubation at room temperature for 24 hours, the number of lactic acid bacteria was measured and compared with the initial number of lactic acid bacteria, the viability of the lactic acid bacteria was confirmed.

However, when the cultured lactic acid bacteria were diluted, the test was carried out by neutralizing with D / E Neutralizing Broth (DIFCO). When the lactic acid bacteria were proliferated in the medium, the number of bacteria on the medium was multiplied by the dilution factor, In the case where the lactic acid bacteria did not proliferate, it was multiplied by the dilution factor <10 (less than 10).

Lactobacillus sakei and Leuconostoc mesenteroides were used as the lactic acid bacteria. In Comparative Example 2, 10 mL of physiological saline contained in a concentration of 1% Lactic acid bacteria viability was measured.

Test Items Initial concentration (CFU / mL) After 24 hours concentration (CFU / mL) Survival rate (%) Lactobacillus
sakei Example 1 1.5 × 10 ⁵ 2.1 × 10 ⁵ 140 Leuconostoc
mesenteroides Example 1 1.0 × 10 ⁵ 1.5 × 10 ⁵ 150 Comparative Example 2 1.0 × 10 ⁵ 0 -

In the case of Comparative Example 2 using a plain white oil as a natural antimicrobial material, all of the lactic acid bacteria were killed after 24 hours because the unbleached oil exhibited antibacterial action against the lactic acid bacteria as well as the antibacterial composition of the present invention It was confirmed that the lactic acid bacteria were rather proliferated unlike Comparative Example 2.

Therefore, in this experiment, it was confirmed that the antimicrobial composition of the present invention has survival and proliferative power of lactic acid bacteria unlike other natural antimicrobial substances.

Antimicrobial Deodorant power  Experiment

20 mL of the antimicrobial composition prepared in the above Preparation Example was sealed in a reactor at 23.0 ± 5.0 ° C. and 50.0 ± 10.0% RH, the initial concentration of the test gas was 50 μmol / mol, The concentration of the test gas was measured to measure the deodorizing power of the antimicrobial composition.

At this time, the result of measurement in the same manner as in the above test method in the absence of a separate sample was set as Comparative Example 3 (control group). Experiment 1, Experiment 2 and Experiment 4 all performed experiments for measuring ammonia deodorization power, Since the experiments were conducted in the environment, they were classified into separate experiments.

Here, the concentration reduction rate is calculated as shown in Equation 1 below.

Test Items Concentration (μmol / mol) 0 minutes 30 minutes 60 minutes 90 minutes 120 minutes Experiment 1 ammonia Example 1 50 One One One <0.2 Comparative Example 3 50 49 49 49 49 Experiment 2 ammonia Example 2 50 One <0.2 <0.2 <0.2 Comparative Example 3 50 49 49 49 49 Experiment 3 Trimethylamine Example 2 50 4 2 One 0.5 Comparative Example 3 50 49 49 49 49 Experiment 4 ammonia Example 3 50 2 One <0.2 <0.2 Example 4 50 2 One <0.2 <0.2 Comparative Example 3 50 49 49 49 49

Test Items Concentration reduction rate (%) 0 minutes 30 minutes 60 minutes 90 minutes 120 minutes Experiment 1 ammonia Example 1 0.0 98.0 98.0 98.0 99.6 Experiment 2 ammonia Example 2 98.0 99.6 99.6 99.6 99.6 Experiment 3 Trimethylamine Example 2 0.0 91.8 95.9 98.0 99.0 Experiment 4 ammonia Example 3 0.0 95.9 98.0 99.6 99.6 Example 4 0.0 95.9 98.0 99.6 99.6

Experimental results of Tables 3, 4 and 6 to 14 show that the examples to which the antimicrobial composition of the present invention is added have the ability to remove ammonia and trimethylamine having odor as compared with Comparative Example 3 .

In particular, when the antimicrobial composition was present, it was confirmed that about 90% or more of the odor-evolved gas was removed after about 30 minutes. Referring to Experiment 1, Experiment 2 and Experiment 4, It can be confirmed that it is similar to that of the high concentration of the antimicrobial composition.

Therefore, through the experiment of the deodorizing ability of the present antimicrobial composition, it was confirmed that the antimicrobial composition has a deodorizing power against ammonia and trimethylamine, which are main substances causing odor in daily life.

[ Experimental Example  2]

Antibacterial performance test of antibacterial wipes

The antimicrobial wet tissue produced in the above Preparation Example was placed in an Erlenmeyer flask, sterilized at 121 ° C for 15 minutes using a sterilizer, and then 1 ml of the cultured test strain was added. Thereafter, the cells were shake cultured at 37.0 ± 0.2 ° C in a 31.7 ± 0.2% R.H environment for 24 hours, and then a certain amount of the cells was plated on an agar medium and then the number of bacteria was measured.

At this time, the test strain was cultured in a nutrient broth and used as the test strains, Super bacteria MRSA (Staphylococcus aureussubsp. Aureus ATCC 33591) and Pseudomonas aeruginosa ATCC 15442.

As Comparative Example 4, the test strain was cultured in the same manner as described above using a stomacher film immersed in purified water, and the number of bacteria was measured.

Test Items Initial concentration (CFU / mL) After 24 hours concentration (CFU / mL) Bacterial reduction rate (%) MRSA Example 5 2.4 × 10 ⁵ <10 99.9 Comparative Example 4 2.4 × 10 ⁵ 3.9 × 10 ⁵ - P. aeruginosa Example 5 3.7 × 10 ⁵ <10 99.9 Comparative Example 4 3.7 × 10 ⁵ 5.5 × 10 ⁵ -

Referring to the results of Table 5 and FIGS. 15 to 18, in the case of Comparative Example 4, the concentration of the test strains was increased 24 hours after the test strain was inoculated, while the antimicrobial wet tissue containing the antimicrobial composition of the present invention In the case of Example 5, it was confirmed that about 99.9% or more of the test strains were killed after 24 hours.

Accordingly, the antimicrobial wet tissue containing the antimicrobial composition according to one embodiment of the present invention can be judged to have antibacterial activity.

[ Experimental Example  3]

Antibacterial performance test of antibacterial fabric (cotton)

Experiments were carried out in the same manner as in the antibacterial performance test of Experimental Example 2 except that the antibacterial fabric prepared in the above Preparation Example was used instead of the antibacterial wet tissue. In this experiment, 5 × 5 cm cotton fabric was used as the fabric and Staphylococcus aureussubsp. Aureus ATCC 33591, Candida albicans ATCC 10231, Staphylococcus aureus ATCC 6538P and Klebsiella pneumonia ATCC 4352) was used.

As Comparative Example 5, the test strain was cultured in the same manner as described above using a stomacher film immersed in purified water, and the number of bacteria was measured.

Test Items Initial concentration (CFU / mL) After 24 hours concentration (CFU / mL) Bacterial reduction rate (%) MRSA Example 6 2.5 x 10 ⁵ <10 99.9 Comparative Example 5 2.5 x 10 ⁵ 4.2 × 10 ⁵ - Candida Example 6 2.5 x 10 ⁵ <10 99.9 Comparative Example 5 2.5 x 10 ⁵ 4.1 × 10 ⁵ - Staphylococcus aureus Example 6 2.3 × 10 ⁵ <10 99.9 Comparative Example 5 2.3 × 10 ⁵ 4.0 × 10 ⁵ - Pneumococcus Example 6 2.8 × 10 ⁵ <10 99.9 Comparative Example 5 2.8 × 10 ⁵ 4.6 × 10 ⁵ -

Referring to the results of Table 6 and FIGS. 19 to 29, in the case of Comparative Example 5, the concentration of the test strains was increased after 24 hours from the inoculation of the test strains, , The concentration of the test strain was less than 10 CFU / mL after 24 hours of inoculation of the test strain and it was confirmed that the bacterial reduction rate was 99.9% or more.

That is, the present inventors confirmed that the antibacterial material comprising the antimicrobial composition according to an embodiment of the present invention has antibacterial activity.

Antimicrobial Antifungal  Performance experiment

The antibacterial fabric prepared in the above Preparation Example was sterilized at 121 DEG C for 15 minutes by using a sterilizer, and 1 mL of the spore suspension of the test strain was inoculated. After standing for 24 hours, a certain amount of the antibacterial fabric was plated on an agar medium, And the number of bacteria was measured.

At this time, Aspergillus niger ATCC 9642 was used as a test strain, a 5 × 5 cm PET fabric was used as the antibacterial fabric, and 5 mL of the antimicrobial composition was mixed with 500 mL of water in Example 7 In Example 8, antibacterial treatment was carried out using an antibacterial treatment liquid in which 2.5 mL of the antibacterial composition was mixed with 500 mL of water.

In Comparative Example 6, antibacterial treatment was performed by mixing 10 mL of benzoyl peroxide (5%), which is a chemical synthetic antimicrobial, in 500 mL of water for dyeing. In Comparative Example 7, PET material which was dyed without any antibacterial treatment Fabric was used.

Test Items Initial concentration (CFU / mL) After 24 hours concentration (CFU / mL) Dying rate (%) Aspergillus
niger Example 7 1.5 × 10 ⁶ 150 99.9 Example 8 1.5 × 10 ⁶ 220 99.9 Comparative Example 6 1.5 × 10 ⁶ 1.2 × 10 ³ 99.9 Comparative Example 7 1.5 × 10 ⁶ 2.1 × 10 ⁵ -

As shown in Table 7, Comparative Example 7, Example 7 and Example 8 showed that the antibacterial fabric of Examples 7 and 8 treated with the antimicrobial composition of the present invention had antibacterial activity against Aspergillus niger .

Comparative Example 6 treated with the chemical synthetic antibacterial agent had antifungal ability, but the performance was remarkably lower than those of Examples.

Experiment on the viability of lactic acid bacteria in antibacterial fabric

The antibacterial fabric prepared in the above Preparation Example was sterilized at 121 DEG C for 15 minutes by using a sterilizer, and 1 mL of lactic acid bacteria was inoculated and allowed to stand for 24 hours. Then, a certain amount of the antibacterial fabric was plated on an agar medium, Respectively.

At this time, the lactic acid bacteria were prepared in the same manner as the lactic acid bacteria viability test of the antimicrobial composition of Experimental Example 1, and Lactobacillus sakei was used as a test strain. The antimicrobial fabric used was a fabric of 5 × 5 cm PET. Examples 7 and 8 were the same as those used in the antifungal test of the above-described antimicrobial fabric. In Comparative Example 8, (5%) was mixed with 500 mL of benzoyl peroxide (5%) and antibacterial treatment was used. In Comparative Example 9, a fabric of 5 × 5 cm PET was used which was dyed without any antibacterial treatment.

Test Items Initial concentration
(CFU / mL) After 24 hours
Concentration (CFU / mL) Survival rate (%) Comparative Example 8 contrast
Survival rate (%) Lactobacillus
sakei Example 7 2.5 × 10 ⁶ 1.8 × 10 ⁶ 72.0 24,324 Example 8 2.2 x 10 ⁶ 1.5 × 10 ⁶ 68.2 20,270 Comparative Example 8 2.2 x 10 ⁶ 74 0.003 - Comparative Example 9 2.2 x 10 ⁶ 150 0.007 2.12

As shown in Table 8, in the case of the examples, the survival rate of the lactic acid bacteria was about 70% after 24 hours of the antibacterial treatment. On the other hand, in the case of Comparative Example 8 treated with the chemical synthetic antibacterial agent and Comparative Example 9 in which no antimicrobial agent was treated, the survival rate of the lactic acid bacteria was almost zero.

In particular, the examples show that the survival rate of lactic acid bacteria is about 200 times or more as compared with that of Comparative Example 9 treated with a chemical synthetic antibacterial agent. When using an antibacterial fiber product made of a fabric subjected to antibacterial treatment with the antibacterial composition of the present invention, Skin allergic reactions such as rash can be significantly improved.

Antimicrobial Deodorant power  Experiment

The antibacterial fabric (1 x 2 cm) prepared in the above Preparation Example was sealed in a reactor under the environment of 22.9 占 0.8 占 폚 and 42.5 占 1.1% RH and injected at an initial concentration of 50 占 퐉 ol / mol of the test gas, The concentration of the test gas was measured every 30 minutes to measure the deodorizing power of the antibacterial fabric.

At this time, the same test was carried out using a specimen in which benzoyl peroxide (5%) treated with 2% of the weight of the fabric was used as Comparative Example 10, The results are shown in Table 9 as Comparative Example 11, and the concentration reduction rate was calculated as shown in Equation 1 below.

Test Items 0 minutes 30 minutes 60 minutes 90 minutes 120 minutes Concentration (μmol / mol) Example 9 50 3 2 2 2 Comparative Example 10 50 42 40 39 38 Comparative Example 11 50 22 20 18 17 Concentration reduction rate (%) Example 9 0.0 92.9 95.0 94.9 94.7 Comparative Example 11 0.0 47.6 50.0 53.8 55.3

As shown in Table 9 and FIGS. 30 to 33, Example 9 showed a deodorization rate of 90% or more with respect to ammonia after 30 minutes of experiment, whereas Comparative Example 11 showed significantly lower deodorization rate than Example 9 after 120 minutes It can be confirmed that the deodorization ratio is about 55%.

Therefore, it can be confirmed that the antimicrobial fabric treated with the antimicrobial composition of the present invention has a significantly higher deodorizing power than the conventional chemical synthetic antimicrobial agent.

As a result, through the above Experimental Examples 1 to 3, the vegetable antibacterial composition according to one embodiment of the present invention, and the antibacterial wipes and antibacterial fabrics which are antibacterial-treated antibacterial fiber products using the same according to one embodiment of the present invention, can be used as Candida, Staphylococcus aureus, Of the present invention has antimicrobial activity against MRSA of superb bacteria as well as general bacteria of the present invention. It has been confirmed that the antimicrobial activity against fungi is enhanced and the viability of lactic acid bacteria is increased or even proliferated.

In addition, it was confirmed that the antimicrobial composition of the present invention and antimicrobial fiber products obtained by using the antimicrobial composition of the present invention are significantly superior to those of commonly used chemical synthetic antimicrobial agents Antifungal ability, deodorizing ability and the like.

The present invention is not limited to the above-described specific embodiments and descriptions, and various modifications can be made to those skilled in the art without departing from the gist of the present invention claimed in the claims. And such modifications are within the scope of protection of the present invention.

Claims (9)

An antibacterial fiber product treated with an antibacterial treatment liquid,
The antimicrobial treatment liquid includes an antimicrobial composition extracted from plants and water,
Wherein the antimicrobial composition comprises minerals and ions. The method according to claim 1,
The mineral includes at least one of calcium (Ca), magnesium (Mg), potassium (K), phosphorus (P), and silicon (Si)
Wherein the ion includes at least one of chloride ion (Cl ^- ), sulfate ion (SO ₄ ^- ) and ammonium ion (NH ₄ ⁺ ). The method according to claim 1,
Wherein the antibacterial fiber product is antibacterial treated using the antibacterial treatment liquid in the form of a yarn or fabric. The method according to claim 1,
The antimicrobial fiber product is at least one selected from the group consisting of a wipe, a wallpaper, a mask, a filter, a medical article, a sanitary article, a clothing, a bedding, a shoe, an automobile seat, a leather product, product. A method for producing an antibacterial fiber product, which comprises applying a antibacterial treatment liquid containing an antibacterial composition extracted from a plant to a fabric, thereby imparting antibacterial property to the fabric. 6. The method of claim 5,
The antimicrobial composition, calcium (Ca), magnesium (Mg), potassium (K), phosphorous (P), includes at least one or more minerals of silicon (Si), sulfate (SO ₄ ^2-), chloride ion ( Cl ^- ), and ammonium ion (NH ₄ ⁺ ). 6. The method for producing an antibacterial fiber product according to claim 5,
Mixing the water and the antimicrobial composition to prepare an antimicrobial treatment liquid, and allowing to stand or agitate for a predetermined time;
Adding a raw material to the antibacterial treatment liquid to perform antibacterial treatment; And
A method for producing an antibacterial fiber product, comprising the steps of charging a dye and heating the dye to fix the dye on the fabric. 6. The method for producing an antibacterial fiber product according to claim 5,
Introducing the fabric into the water;
Adding the antimicrobial composition to the water, and allowing to stand or agitate for a predetermined time; And
A method for producing an antibacterial fiber product, comprising the steps of charging a dye and heating the dye to fix the dye on the fabric. 6. The method for producing an antibacterial fiber product according to claim 5,
Immersing the fabric in a first water containing the dye and heating the fabric for a predetermined time to fix the dye on the fabric;
Removing the fabric from the first water containing the dye, washing with water and drying to obtain a dyed fabric; And
Applying the dyed fabric to a second water containing the antimicrobial composition and the additive, thereby antibacterial and padding the dyed fabric.

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