KR20210000009A - Manufacturing method water-soluble hydrofiber having antibiotic and crosslinkability and wound dressing using the water-soluble hydrofiber - Google Patents

Abstract

The present invention prepares a mixed solution by adding an aluminum compound, a zinc compound, a silver compound, and a cobalt compound to the chitosan solution, and by dipping and reacting a carboxylated nonwoven fabric in the mixed solution at room temperature, the amine group-metal-carboxyl group of chitosan A method for producing a water-soluble hydrofiber nonwoven fabric having high antibacterial properties and increasing gel strength by crosslinking a carboxylated nonwoven fabric by providing antibacterial properties and crosslinking properties simultaneously by chelate reaction of, and a water-soluble hydrofiber nonwoven fabric prepared therefrom. It is about round dressing.

Description

Manufacturing method of water-soluble hydrofiber nonwoven fabric that gives antibacterial and crosslinking properties at the same time, and Wound dressing using water-soluble hydrofiber nonwoven fabric manufactured therefrom {MANUFACTURING METHOD WATER-SOLUBLE HYDROFIBER HAVING ANTIBIOTIC AND CROSSLINKABILITY AND WOUND DRESSING USING THE WATER-SOLUBLE HYDROFIBER}

The present invention prepares a mixed solution by adding an aluminum compound, a zinc compound, a silver compound, and a cobalt compound to the chitosan solution, and by dipping and reacting a carboxylated nonwoven fabric in the mixed solution at room temperature, the amine group-metal-carboxyl group of chitosan A method for producing a water-soluble hydrofiber nonwoven fabric having high antibacterial properties and increasing gel strength by crosslinking a carboxylated nonwoven fabric by providing antibacterial properties and crosslinking properties simultaneously by chelate reaction of, and a water-soluble hydrofiber nonwoven fabric prepared therefrom. It is about round dressing.

With the increasing use of wound healing products with high wound regeneration effects in traditional gauze-type wound care products around the world, the wound dressing market is expected to grow 3.1%, which is higher than 2.2% of existing wound care products.

In the domestic market, the use of wound coverings is also expanding due to the changing perception of wound healing. With the recent rapid increase in production (30.8%) and usage rate (38.2%) of domestic products, fierce market competition between domestic and foreign companies is in progress in the domestic market.

In general, wound healing is much faster when the moisture environment is maintained than in a dry state. Among the currently used dressings, hydrogel is a material used for burn treatment or skin regeneration that requires a constant moist state, and can achieve its purpose only when it contains more than 60% moisture.

In the case of severe burn treatment, autograft or in vivo culture of fibroblasts from patients is transplanted. However, it is essential to prevent infection of the affected area before the procedure because it takes a considerable amount of time to perform the above procedure.

Various types of wound covering agents are being developed depending on the shape of the wound, and in Korea, dressing agents such as foam, film, and hydrogel are mainly developed and produced. Overseas multinational companies have developed and sold a variety of products using hydrogel, alginate, hydrofiber, and polyurethane foam as more advanced forms.

In general, in order to manufacture a wettable foam dressing agent or hydrogel, a polymer capable of forming a hydrogel must be selected, and it does not react sensitively to changes in the external environment (temperature, pH, solvent composition, electric field, light intensity, chemicals, etc.). It should not show changes in physical properties such as moisture content.

Foam dressing agents and hydrogels, which are currently used dressing agents, mostly remain wet or dissolved by exudates, so that residues adhere to the wound area, so there is a problem that the wound area must be cleaned secondarily. In addition, since the foam dressing agent is made of urethane, it is difficult to impart antibacterial properties or substances capable of promoting wound healing, and even in the case of a hydrogel, it is difficult to impart antibacterial properties in the process.

In order to solve such a problem, due to the nature of the polymer structure, it absorbs water by capillary and osmotic pressure to contain moisture, and does not dissolve in exudate or water through electrostatic, lipophilic interaction and proper crosslinking between polymer chains. There is a need to develop a dressing agent that has characteristics and contains substances that are useful for antibacterial properties and wound healing.

Looking at the present invention and the conventionally disclosed patent technologies, US Patent No. 5,389,376 discloses a method of manufacturing a dressing for wound treatment using a radiation crosslinking method. Agar and polyethylene oxide are mixed with polyvinylpyrrolidone, and this A technique obtained by irradiation and crosslinking is disclosed.

Compared with the present invention, there is a characteristic of the radiation crosslinking method, that is, crosslinking and sterilization can be simultaneously promoted, but the strength of the hydrogel is low when mixing polyvinylpyrrolidone and agar, and the strength of the hydrogel is poor. Is weak and has a problem of tearing.

In the case of US Patent No. 5,480,717, a hydrogel prepared by casting an aqueous solution of polyvinylpyrrolidone on a polymer film with an adhesive and irradiating with radiation is disclosed.

Compared to the present invention, the hydrogel has a weak strength, but has a problem in that polyvinylpyrrolidone used when preparing the hydrogel remains in the wound area due to too strong adhesiveness.

Japanese Patent Application Laid-Open No. 9-267453 discloses a technique for improving physical properties by using polyvinyl alcohol as a basic material and adding another laminating agent thereto.

Compared with the present invention, since the product is manufactured simply by irradiation, there is a limit to improvement in physical properties, and since it cannot be put in a packaging material while maintaining its shape without irradiation, radiation must be irradiated twice, When used for a long time on the affected area, there is a problem that an antibacterial agent must be used separately.

In addition, Korean Patent Laid-Open Publication No. 2001-0086864 describes the steps of preparing an aqueous solution by mixing a polyvinylpyrrolidone synthetic polymer with chitosan, chitosan and polyethylene oxide or sodium alginate and polyethylene oxide, forming a sheet, and then packaging Discloses a technology consisting of the step of irradiating radiation on the sheet,

Korean Patent Laid-Open No. 2004-0085646 discloses a hydrogel dressing comprising a composition consisting of polyvinylpyrrolidone, polyhydric alcohol and carrageenan, a tray, and a method of manufacturing the same by irradiation with radiation, and a wound treatment dressing or skin beauty pack using the same Are doing.

In addition, Republic of Korea Patent Registration No. 10-1318421 is a carboxymethyl cellulose foam for hemostasis and wound healing and its manufacturing method. After manufacturing a carboxymethyl cellulose foam by a freeze-drying method, the liquid absorption of the foam is carried out by performing an appropriate acid treatment and compression step. Was significantly increased, and a technique for maintaining the shape as it is without gelation even when absorbed is disclosed.

Comprehensive review, the conventionally disclosed technologies have a short usable time problem, and when exposed to air for more than 12 hours, moisture is evaporated and thus cannot function as wound healing, and the manufacturing process is complex and manufacturing cost is high. there is a problem.

In addition, there are technical problems that require treatment with antibacterial agents, etc., and the absorption of exudate is insufficient.

In order to solve such a problem, the applicant of the present invention reads ``A water-soluble hydrofiber manufacturing method, a round dressing using a water-soluble hydrofiber prepared therefrom'' through Korean Patent Publication No. 10-2018-0041980 (published on April 25, 2018). Has disclosed a description of.

The above disclosed patent discloses a technology for imparting antimicrobial properties by dipping and reacting an already crosslinked hydrofiber nonwoven fabric or woven fabric in a third mixed solution (a solution obtained by adding an aluminum compound or a calcium compound to a chitosan solution and dissolving it).

The applicant of the present invention has conducted a number of studies and experiments on a method to improve the antibacterial properties of the hydrofiber nonwoven fabric. As a result, the crosslinking reaction occurs and at the same time by dipping and reacting the carboxylated nonwoven fabric in the mixed solution presented in the present invention. The antimicrobial properties were simultaneously imparted, and the water-soluble hydrofiber nonwoven fabric with high antibacterial properties was developed.

An object of the present invention is to provide a water-soluble hydrofiber nonwoven fabric having high antimicrobial properties by providing antibacterial properties at the same time as the crosslinking reaction occurs, and a round dressing using the water-soluble hydrofiber nonwoven fabric.

US Patent No. 5,389,376 (Registration Date 1995.02.14) US Patent No. 5,480,717 (Registration Date 1996.01.02) Japanese Laid-Open Patent No. 9-267453 (published date 1997.10.14) Republic of Korea Patent Publication No. 2001-0086864 (published date 2001.09.15) Republic of Korea Patent Publication No. 2004-0085646 (published date 2004.10.08) Korean Patent Registration No. 10-1318421 (Registration Date 2013.10.08) Republic of Korea Patent Publication No. 10-2018-0041980 (Publication date 2018.04.25)

The present invention prepares a mixed solution by adding an aluminum compound, a zinc compound, a silver compound, and a cobalt compound to the chitosan solution, and by dipping and reacting a carboxylated nonwoven fabric in the mixed solution at room temperature, the amine group-metal-carboxyl group of chitosan A method for producing a water-soluble hydrofiber nonwoven fabric having high antibacterial properties and increasing gel strength by crosslinking a carboxylated nonwoven fabric by providing antibacterial properties and crosslinking properties simultaneously by chelate reaction of, and a water-soluble hydrofiber nonwoven fabric prepared therefrom. It is an object of the invention to provide a round dressing.

To achieve the above purpose,

The present invention in the antibacterial hydrofiber nonwoven fabric manufacturing method using a pure water-soluble carboxylated hydrofiber nonwoven fabric obtained by carboxylating the nonwoven fabric made of cotton or rayon as it is,

A step (S10) of preparing a chitosan-metal mixed solution by adding and dissolving at least one metal selected from an aluminum compound, a zinc compound, a silver compound, or a cobalt compound to the chitosan solution; and

The step of immersing and reacting the carboxylated hydrofiber nonwoven fabric in the chitosan-metal mixed solution at 35 to 45° C. for 2 to 10 minutes to give crosslinking and antibacterial properties at the same time (S20),

After dehydrating the nonwoven fabric with antimicrobial properties, the process of dipping the nonwoven fabric subjected to the dehydration process in a mixed solution of water and ethanol, and repeating the dehydration process 2 to 4 times (S30), a method for producing a water-soluble hydrofiber nonwoven fabric Provides.

And it provides a round dressing, characterized in that the water-soluble hydrofiber manufactured through the method of manufacturing the water-soluble hydrofiber nonwoven fabric is cut according to a desired standard.

The present invention has high antibacterial properties as it can provide antibacterial properties at the same time as crosslinking by dipping and reacting a carboxylated nonwoven fabric in a mixed solution prepared by adding a metal aluminum compound, a zinc compound, a silver compound, and a cobalt compound to a chitosan solution. In addition, it has the effect of increasing the gel strength by crosslinking the carboxylated nonwoven fabric.

1 is a process flow chart according to the method of manufacturing a water-soluble hydrofiber nonwoven fabric of the present invention.

The technical configuration according to the present invention will be described in detail together with the drawings.

As shown in Figure 1,

The method for producing a water-soluble hydrofiber nonwoven fabric according to the present invention,

A pure water-soluble carboxylated hydrofiber nonwoven fabric obtained by carboxylating a nonwoven fabric made of cotton or rayon as it is,

A step (S10) of preparing a chitosan-metal mixed solution by adding and dissolving at least one metal selected from an aluminum compound, a zinc compound, a silver compound, or a cobalt compound to the chitosan solution; and

The step of immersing and reacting the carboxylated hydrofiber nonwoven fabric in the chitosan-metal mixed solution at 35 to 45° C. for 2 to 10 minutes to give crosslinking and antibacterial properties at the same time (S20),

After dehydrating the nonwoven fabric with antimicrobial properties, the process of dipping the nonwoven fabric subjected to the dehydration process in a mixed solution of water and ethanol is repeated 2 to 4 times (S30).

Hereinafter, the technical configuration of each step of the method for manufacturing the water-soluble hydrofiber nonwoven fabric will be described in more detail.

1. Chitosan-metal mixed solution manufacturing step (S10)

The chitosan-metal mixed solution is prepared by adding one or two or more metals selected from an aluminum compound, a zinc compound, a silver compound, or a cobalt compound to the chitosan solution.

As the chitosan solution, chitosan having a molecular weight of 50,000 to 200,000 is dissolved in a mixed solution of water and ethanol, and the chitosan is dissolved in a concentration of 0.5 wt% to 3.0 wt%.

When the concentration of chitosan is less than 0.5 wt%, the amount of chitosan is too small to produce antimicrobial or crosslinking properties, and when it exceeds 3.0 wt%, the viscosity is too high to enter the carboxylated nonwoven fabric after reaction. Since there is a disadvantage to be washed, the concentration of chitosan is preferably maintained within the range of 0.5 wt% to 3.0 wt%.

The amount of the metal used is limited to within the range of equal to 1/32 mole of the carboxyl group of the nonwoven fabric and the amine group of chitosan.

It is preferably limited to within the range of 1/4 to 1/16 mol.

When the amount is greater than the same mole, there is a problem that the crosslinking property does not appear, and when the amount is less than 1/32 mole, the antibacterial property and gel strength are too small, so the shape of the nonwoven fabric collapses when the nonwoven fabric absorbs water or blood. , It is preferable that the amount of the metal is limited within the range of the same mole to 1/32 mole with respect to the carboxyl group of the nonwoven fabric and the amine group of chitosan.

Among the metals, the aluminum compound is a compound consisting of one or two or more monovalent acids selected from aluminum nitrate, aluminum acetate, aluminum nitrate, or aluminum chloride,

The zinc compound is zinc nitrate, zinc acetate, zinc nitrate or zinc chloride,

The silver compound uses silver oxide or silver nitrate,

The cobalt compound is one or two or more selected from cobalt nitrate, cobalt chloride, or cobalt acetate.

At this time, it is preferable to use aluminum nitrate, zinc oxide, silver oxide, or cobalt chloride, and two or more compounds may be used together.

The reason for limiting the mixing ratio of each component is that the ligand that can react with the carboxyl group and the amine group is limited, and by maintaining an appropriate degree of crosslinking between chitosan and the carboxylated nonwoven fabric by chelate reaction, the nonwoven fabric has absorbency, hemostatic properties and antibacterial properties It is for sake.

2. Step of dipping and reacting a carboxylated hydrofiber nonwoven fabric in a chitosan-metal mixed solution (S20)

The carboxylated hydrofiber nonwoven fabric is a pure water-soluble carboxylated hydrofiber nonwoven fabric manufactured by carboxylating a nonwoven fabric made of cotton or rayon as it is.

The cotton (Cotton) refers to the staple fiber (staple fiber) attached to the cotton seed.

The rayon refers to a pulp made of pure fiber by chemically treating wood or cotton debris, and chemically dissolving it to solidify it into fibrous form. The pulp is obtained by extracting the fiber of a plant by mechanical or chemical treatment, and is mainly used as a raw material for fiber or paper.

A specific example of preparation of the pure water-soluble carboxylated hydrofiber nonwoven fabric is as follows.

1 kg of NaOH was dissolved in 12 L of an aqueous ethanol solution in which water and ethanol were mixed in a volume ratio of 3:7, and 500 g of a nonwoven fabric made of cotton was immersed and reacted at room temperature at 25°C for 24 hours. 1 kg of monochloroacetic acid was added to the reaction product and reacted for 5 hours to prepare a carboxylated nonwoven fabric. The carboxylated nonwoven fabric was washed three times to prepare a pure carboxylated cotton nonwoven fabric. At this time, the carboxylation rate of the cotton nonwoven fabric is 80%.

The nonwoven fabric is a fiber that is mechanically, chemically or thermally treated to bind it into a fiber aggregate to form a fabric directly, and is also referred to as a bonded fabric.

In this step (S20), the pure water-soluble carboxylated hydrofiber nonwoven fabric is immersed and reacted in the chitosan-metal mixed solution obtained in the previous step (S10), but the temperature of the chitosan-metal mixed solution is maintained at 35 to 45°C. This is a step to simultaneously impart antimicrobial properties and crosslinking properties by chelating reaction of an amine group-metal-carboxyl group of chitosan by immersion and reaction for 2 to 10 minutes in one state.

3. Step of immersion and dehydration in a mixed solution of water and ethanol (S30)

This step (S30) is to complete the water-soluble hydrofiber nonwoven fabric according to the present invention by dehydrating the nonwoven fabric provided with antibacterial properties in the previous step (S20), and then immersing and dehydrating the nonwoven fabric subjected to the dehydration process in a mixed solution of water and ethanol. Step.

At this time, the deposition and dehydration processes are repeated 2 to 4 times. More specifically, it repeats 3 times.

The mixed solution of water and ethanol is a specific example, and a mixed solution of pure water and ethanol in a volume ratio of 3:7 is used.

A specific example of manufacturing the water-soluble hydrofiber nonwoven fabric will be described through Examples.

200 g (0.25 mol) of chitosan was dissolved in 20 L of a mixed solution of water and ethanol in a volume ratio of 3:7, and 58.59 g (0.16 mol) of aluminum nitrate was dissolved therein.

Next, 1 kg of the carboxylated nonwoven fabric shown in Preparation Example was immersed for 5 minutes while maintaining the temperature at 40°C.

Then, the carboxylated cotton nonwoven fabric is taken out, dehydrated with a dehydrator, and then immersed in 20 L of a mixed solution in which pure water and ethanol are mixed in a volume ratio of 3:7-and dehydration is performed three times, so that the amine group of chitosan according to the present invention-metal- A water-soluble hydrofiber having a chelating reaction of a carboxyl group is completed.

In place of the aluminum nitrate of Example 1, a water-soluble hydrofiber was prepared in the same manner as in Example 1, except that 3.3 g (0.02 mol) of silver nitrate was used.

A water-soluble hydrofiber was prepared in the same manner as in Example 1, except that 4.3 g (0.02 mole) of zinc acetate was used in place of the aluminum nitrate of Example 1.

In place of the aluminum nitrate of Example 1, a water-soluble hydrofiber was prepared in the same manner as in Example 1, except that 1.3 g (0.01 mol) of silver nitrate and 2.2 g (0.01 mol) of zinc acetate were used.

[Test Example 1]

[Measurement of antibacterial activity]

The antibacterial properties of the water-soluble hydrofiber nonwoven fabric obtained by chelation of chitosan and metal were measured by ASTM E2149-10. The measurement method is as follows.

① Put the finished product in a petri dish aseptically.

② Inoculate the sample with 1 mL of at least 10 ⁶ cfu/mL of bacterial solution and incubate at the designated temperature.

③ Set the contact time, mix the sample with the neutralization solution according to the specified time, and separate the bacteria from the sample using a Stomacher.

④ Dilute 1　mL of the bacteria solution appropriately and paint using the Pour plating method or the Spread plating method.

⑤ Incubate for 24 　 hours or 48 　 hours at the designated temperature and measure the number of viable cells.

⑥ Calculate the reduction rate using the formula below.

Log ₁₀ reduction (LR) = mean log ₁₀ (number of initial bacteria)-mean log ₁₀ (number of surviving bacteria)

Percent reduction(%) = 100 × (1-10 ^-LR )

Results according to the antimicrobial measurement are shown in Tables 1 to 4 below.

Table 1 below shows the results according to Example 1.

Table 2 below shows the results according to Example 2.

Table 3 below shows the results according to Example 3.

Table 4 below shows the results according to Example 4.

Test Items Initial concentration
(CFC/mL) Concentration after 24 hours
(CFC/mL) Bacterial reduction rate (%) Coli BLANK 1.7×10 ⁴ 5.9×10 ⁴ 99.9 Antibacterial treatment 1.4×10 ⁴ <10 Staphylococcus aureus BLANK 1.3×10 ⁴ 2.2×10 ⁴ 99.9 Antibacterial treatment 1.1×10 ⁴ <10 Salmonella BLANK 4.2×10 ⁵ 6.0×10 ⁵ 99.9 Antibacterial treatment - <10 Pseudomonas aeruginosa BLANK 5.2×10 ⁵ 9.2×10 ⁵ 99.9 Antibacterial treatment - <10

Test Items Initial concentration
(CFC/mL) Concentration after 24 hours
(CFC/mL) Bacterial reduction rate (%) Coli BLANK 1.7×10 ⁴ 6.9×10 ⁴ 99.9 Antibacterial treatment 1.7×10 ⁴ <10 Staphylococcus aureus BLANK 1.3×10 ⁴ 4.2×10 ⁴ 99.9 Antibacterial treatment 1.3×10 ⁴ <10 Salmonella BLANK 4.2×10 ⁵ 7.0×10 ⁵ 99.9 Antibacterial treatment - <10 Pseudomonas aeruginosa BLANK 5.2×10 ⁵ 9.2×10 ⁵ 99.9 Antibacterial treatment - <10

Test Items Initial concentration
(CFC/mL) Concentration after 24 hours
(CFC/mL) Bacterial reduction rate (%) Coli BLANK 2.7×10 ⁴ 8.9×10 ⁴ 99.9 Antibacterial treatment 1.7×10 ⁴ <10 Staphylococcus aureus BLANK 1.3×10 ⁴ 6.2×10 ⁴ 99.9 Antibacterial treatment 1.3×10 ⁴ <10 Salmonella BLANK 2.2×10 ⁵ 6.0×10 ⁵ 99.9 Antibacterial treatment - <10 Pseudomonas aeruginosa BLANK 5.2×10 ⁵ 8.2×10 ⁵ 99.9 Antibacterial treatment - <10

Test Items Initial concentration
(CFC/mL) Concentration after 24 hours
(CFC/mL) Bacterial reduction rate (%) Coli BLANK 2.7×10 ⁴ 8.9×10 ⁴ 99.9 Antibacterial treatment 1.7×10 ⁴ <10 Staphylococcus aureus BLANK 1.3×10 ⁴ 7.2×10 ⁴ 99.9 Antibacterial treatment 1.3×10 ⁴ <10 Salmonella BLANK 1.2×10 ⁵ 9.0×10 ⁵ 99.9 Antibacterial treatment - <10 Pseudomonas aeruginosa BLANK 2.2×10 ⁵ 8.2×10 ⁵ 99.9 Antibacterial treatment - <10

As a result of the measurement, as confirmed through Tables 1 to 4, the antimicrobial activity of 99.99% was exhibited in all examples.

[Test Example 2]

[Absorption test]

A round dressing sample was prepared with water-soluble hydrofiber (5cm×5cm), placed in a petri dish, and weighed (W ₁ ). After accurately measuring distilled water warmed to 37 (±1) °C to ±0.5 g, 40 times the weight of the sample is added in consideration of the absorption power of the sample.

Then, after leaving it in a thermostat at 37 (±1) °C for 30 minutes, hang the round dressing sample for 30 seconds using tweezers, measure the weight (W ₂ ), and measure the amount of absorption using the following calculation formula. The results are shown in Table 5 below.

Absorption amount = (W ₂ -W ₁ /W ₁ ) × 100

Here, W ₁ : Initial weight (g)

W ₂ : Wound dressing sample weight after 30 minutes (g)

Absorption amount unit:%

division Example 1 Example 2 Example 3 Example 4 W ₁ (g) 0.64 0.64 0.63 0.64 W ₂ (g) 12.94 10.90 12.4 11.52 Absorption (%) 1922 1603 1868 1700

[Test Example 3]

[Hemostasis Characteristics Test]

Nine rats weighing between 250 and 300 g were tested. Group A is an experimental group using the round dressing made of the water-soluble hydrofiber of the present invention (Example 4), Group B is a positive control group, a dressing agent in the form of an existing foam (Mediform), and Group C is a negative control group. Dressing was done using gauze.

Cut the wound into a size of 1.0×1.0(cm) with a surgical knife on the test site, cut it into 1.5×1.5(cm) dressings, cover it on the bleeding area, and then put a 200 g weight to stop the bleeding time for 30 seconds and 60 I saw 4 items in seconds, 90 seconds, and over 90 seconds (failure). Each of 5 animals was tested (Table 6).

division 30 seconds 60 seconds 90 seconds More than 90 seconds Group A (experimental group) One 3 One 0 Group B (positive control group) One 3 One 0 Group C (negative control group) 0 One One 3

As can be seen in Table 5, more than 90 seconds were reported as failure and the experimental group had a hemostatic rate of 100%, the positive control group 100%, and the negative control group 40%.

The water-soluble hydrofiber wound coating agent of the present invention quickly forms a gel when blood is released, absorbs blood, and forms a rapid membrane by gelation of the applied hydrofiber, so that cations of chitosan are combined with anions on the surface of red blood cells, thereby bleeding rapidly. Was judged.

The water-soluble hydrofiber according to the present invention, and the wound dressing manufactured using the same, rapidly absorb exudate from acute and chronic wounds, severely damaged wounds and infected wounds, maintain a moist environment, and have antimicrobial properties, rapid hemostasis and wound healing synergistic effect As a superabsorbent surgical dressing agent having a high industrial applicability.

Claims (5)

In the antibacterial hydrofiber nonwoven fabric manufacturing method using a pure water-soluble carboxylated hydrofiber nonwoven fabric obtained by carboxylating the nonwoven fabric woven with fibers made of cotton or rayon as it is,
A step (S10) of preparing a chitosan-metal mixed solution by adding and dissolving at least one metal selected from an aluminum compound, a zinc compound, a silver compound, or a cobalt compound to the chitosan solution; and
The step of immersing and reacting the carboxylated hydrofiber nonwoven fabric in the chitosan-metal mixed solution at 35 to 45° C. for 2 to 10 minutes to give crosslinking and antibacterial properties at the same time (S20),
A water-soluble hydrofiber comprising the step (S30) of dehydrating the nonwoven fabric with antimicrobial properties, immersing the nonwoven fabric subjected to the dehydration process in a mixed solution of water and ethanol, and repeating the dehydration process 2 to 4 times (S30). Non-woven manufacturing method.
The method according to claim 1,
Chitosan solution is a method for producing a water-soluble hydrofiber nonwoven fabric, characterized in that dissolving chitosan having a molecular weight of 50,000 to 200,000 in a mixed solution of water and ethanol, and dissolving the chitosan concentration to be 0.5 wt% to 3.0 wt%.
The method according to claim 1,
A method for producing a water-soluble hydrofiber nonwoven fabric, characterized in that the amount of metal is in the range of equal to 1/32 mole with respect to the carboxyl group of the nonwoven fabric.
The method according to claim 1,
A method for producing a water-soluble hydrofiber nonwoven fabric, characterized in that the amount of metal is in the range of equimolar to 1/32 mol with respect to the amine group of chitosan.
Wound dressing, characterized in that the water-soluble hydrofiber manufactured through the manufacturing method of any one of claims 1 to 4 is cut to a desired standard.

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