KR20210018388A - Compositions for improving skin conditions comprising plant extracts or fractions thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin condition containing a mixed extract of two or more samples of marshmallow, calendula, and adult motherwort or a fraction thereof as an active ingredient, and specifically, the present invention is from the group consisting of marshmallow, calendula and St. It relates to a cosmetic composition, a food composition, a quasi-drug composition, and a pharmaceutical composition containing a mixed extract of two or more selected samples or a fraction thereof as an active ingredient.
The composition containing a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula, and St. Mary's plant of the present invention or a fraction thereof as an active ingredient is excellent in skin regeneration, skin elasticity effect, and skin wrinkles, skin aging, skin It is also excellent in preventing, improving or treating inflammation and skin pigmentation. Specifically, the composition of the present invention is excellent in the effect of increasing the total amount of collagen and suppressing the inflammatory reaction.

Description

Compositions for improving skin conditions comprising plant extracts or fractions thereof as an active ingredient {Compositions for improving skin conditions comprising plant extracts or fractions thereof}

The present invention relates to a cosmetic composition for improving skin condition containing a mixed extract of two or more samples of marshmallow, calendula, and adult motherwort or a fraction thereof as an active ingredient, and specifically, the present invention is from the group consisting of marshmallow, calendula and St. It relates to a cosmetic composition, a food composition, a quasi-drug composition, and a pharmaceutical composition containing a mixed extract of two or more selected samples or a fraction thereof as an active ingredient.

Cosmetics can be broadly classified into basic cosmetics for supplying nutrients to the skin and color cosmetics in which pigments that are not harmful to the human body are mixed to express the contours in a unique way. In particular, basic cosmetics include emulsions, lotions, skins, lotions, and the like, and most women and men use basic cosmetics. These basic cosmetics not only cleanse the skin by removing dead skin cells and impurities from the skin, but also form a thin protective film while soothing the skin damaged by cleansing by controlling oil and moisture in a balanced manner, thereby preventing skin troubles.

In addition, in recent years, as income increases, the standard of living has improved, leading to more investments in cosmetics to enhance one's image. And as the elderly population increases, interest and demand for functional cosmetics related to anti-aging, wrinkle improvement, whitening, and sun protection are increasing. However, since most of these basic cosmetics or functional cosmetics compositions are made of chemical substances, skin troubles often occur when applied to sensitive skin.

On the other hand, active oxygen introduced from outside the living body or generated in the living body causes many problems such as promoting aging in the living body or causing cancer. Therefore, many studies and developments have been made on antioxidant substances that inhibit oxidation by active oxygen. Conventionally, there have been many efforts to obtain anti-skin aging effects by combining antioxidants such as superoxide dismutase (SOD), vitamin E derivatives, ascorbic acid and derivatives thereof, and flavonoids in cosmetics. When compounding, a problem occurs in terms of stability, and its use is limited.

Inflammation is a series of defense purposes to minimize the response and restore the damaged area to the original state when cells or tissues are damaged by any cause, causing nerve and blood vessels, lymphatic ducts, fluid reactions, and cellular reactions, resulting in pain, It causes swelling, redness, and fever, which causes dysfunction. The causes of inflammation include physical factors such as trauma, frostbite, burns, radioactivity, chemical factors such as acids, and immunological factors due to antibody reactions, and also caused by blood vessel or hormonal imbalance. do. Vascular dilation occurs by various chemical mediators secreted by cells damaged by external stimulation, and as permeability increases, antibodies, complement, plasma, and phagocytic cells flock to the inflammatory site. This phenomenon is the cause of erythema.

In order to eliminate inflammation, it is called anti-inflammatory drugs that remove inflammatory sources, reduce biological reactions and symptoms. Substances used for anti-inflammatory purposes to date include flufenamic acid, ibuprofen, benzydamine, indomethacin, etc. as non-steroids, and prednisolone as a steroid system. ), dexamethasone, etc., but their use is limited because most of them have problems in terms of safety for the skin or in terms of stability when containing cosmetics.

Therefore, in recent years, cosmetic compositions containing plant extracts of natural materials, which are excellent in moisturizing, anti-aging, antioxidant, and anti-inflammatory effects, have excellent effects in improving skin conditions or preventing skin aging or skin damage. There is a lot of research going on. However, in the case of using the plant extract alone, it often shows insignificant skin improvement effect.

Under this background, the present inventors have conducted research on a substance that is non-toxic and has excellent skin condition improvement effect from natural resources, and thus, mixing of two or more samples selected from the group consisting of marshmallow, calendula, and St. The present invention was completed by confirming the effect of improving the skin condition through the effect of promoting collagen synthesis and suppressing nitrogen monoxide production of the extract or its fractions.

It is an object of the present invention to provide a cosmetic composition for improving skin conditions, containing as an active ingredient a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula and St.

Another object of the present invention is to provide a quasi-drug composition, a food composition, and a pharmaceutical composition, containing as an active ingredient a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula and St.

As an aspect for achieving the above object, the present invention provides a cosmetic composition for improving skin condition containing as an active ingredient a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula, and St. to provide.

In the present invention, "Marshmallow ( Althaea Officinalis )" is a perennial herb with high value for use as a medicinal or horticultural plant. It is also called Pelargonium, and leaves, flowers, and roots are all used for medicinal purposes. The leaves are effective in analgesics, expectorants, and diuretics when they bloom in summer. Are harvested in late autumn.

Of the present invention "karendyulra (Calendula Officinalis )" is a perennial plant of the Asteraceae family, also known as marigold, marigold, and snail marigold. It has a height of 30 to 50cm and blooms in May to October. Due to its cold resistance, it is shipped from December to spring when sown in autumn in the open field. Calendula is well known as a topically applied herb to treat cuts, abrasions, etc. It has also been known to be effective in promoting digestion, relieving indigestion, gastric ulcer, and duodenal ulcer.

" Alchemilla " of the present invention Vulgaris )" is a herb plant of the rose family native to Europe, also called lady's mantle. There are fine hairs on the stem and leaves, and the leaves are round on the kidney bottom and are shallowly divided into 7 to 11 pieces. The roots and leaves are for edible or medicinal purposes. It has a lot of tannins and has astringent action, so it has been used for wound healing and menstruation.

The term "extract" as used in the present invention refers to an extract obtained by extracting plants, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof, It includes the extract itself and extracts of all formulations that can be formed using the extract. The extract of the present invention may be preferably prepared and used in the form of a dry powder after extraction.

The extract of the present invention may be extracted from natural, hybrid or variant plants of the respective plants, and may be extracted from plant tissue culture.

Two or more kinds of mixed extracts selected from the group consisting of the marshmallow, calendula, and St. Mary of the present invention are prepared by extracting each of the marshmallow, calendula and St. Mary, and then mixing two or more kinds of each extract, or the marshmallow, calendula and It can be prepared by extracting two or more selected from the group consisting of St. When mixing each extract, each extract was mixed in the same amount.

In the present invention, the type of the extraction solvent used to extract the plant is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include one or more solvents selected from the group consisting of water, alcohol, 1,3-butylene glycol, and ethyl acetate, and more preferable when alcohol is used as a solvent. Preferably, alcohols of C ₁ to C ₄ may be used. In the present invention, more preferably, water (or distilled water) or ethanol may be used as a solvent for extracting each plant.

In the extracts of the respective plants of the present invention, the method of extracting the respective plants is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include vacuum low temperature extraction, room temperature extraction, hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, etc., which are performed alone or in combination of two or more methods. Can be done.

The extract of the present invention can be more preferably extracted by a vacuum low temperature extraction method. Vacuum low temperature extraction is a method of extracting using an extraction solvent under vacuum under low temperature conditions.

In the case of extraction by the vacuum low-temperature extraction method in the present invention, it is preferable to repeatedly extract once to five times, and more preferably, extract repeatedly three times, but is not limited thereto. The extraction solvent may be added 0.1 to 10 times the weight of the dried plant, and it is preferable to add 0.3 to 5 times. The extraction temperature may be a low temperature of 5 ℃ to 70 ℃, preferably 5 ℃ to 40 ℃, more preferably 15 ℃, the extraction time is preferably 12 to 48 hours, but is not limited thereto. In the case of extraction by the vacuum low temperature extraction method, there is an advantage of improving the extraction efficiency without destruction or loss of the active ingredient, and furthermore, when the mixed extract is prepared by the vacuum low temperature extraction method, the skin condition is improved by the cooperative action between the active ingredients. , There is an advantage of becoming more excellent in skin disease treatment efficacy.

The extract of the plant of the present invention may be concentrated under reduced pressure using a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, the drying is preferably vacuum drying, vacuum drying, boiling drying, spray drying, or freeze drying, but is not limited thereto.

As used herein, the term "fraction" means a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing several different constituents.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method include a method of obtaining a fraction from the extract by treating an extract obtained by extracting two or more selected from the group consisting of marshmallow, calendula, and St.

In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; Non-polar solvents, such as hexane, ethyl acetate, chloroform, and dichloromethane, etc. are mentioned. These may be used alone or in combination of two or more. In the case of using an alcohol in the fractionation solvent, preferably a C ₁ to C ₄ alcohol may be used.

According to a specific preparation example of the present invention, the roots of marshmallows, flowers of calendula, and leaves of Stomatalus are washed with water and dried in the shade, and then each of the dried plants is pulverized and put into an extraction container, followed by 15° C. with an extraction solvent. Extraction was obtained by repeated extraction under vacuum at low temperature of. The obtained extract was filtered to remove solids using filter paper or the like, and then the extract was concentrated under reduced pressure to prepare an extract (Preparation Example 1).

A single extract such as marshmallow, calendula, and St. Maryland extract, and marshmallow and calendula mixed extract, a mixed extract of two or more samples thereof, a mixed extract of calendula and St. (Table 1).

As another aspect for achieving the above object, the present invention provides a method for preparing a cosmetic composition for improving skin condition containing as an active ingredient two or more mixed extracts or fractions thereof selected from the group consisting of marshmallow, calendula and St. to provide.

Specifically, the method includes extracting two or more types of samples selected from the group consisting of marshmallow, calendula, and St. Mary's using an extraction solvent under low temperature vacuum. The extraction temperature may be a low temperature of 5 ℃ to 70 ℃, preferably 5 ℃ to 40 ℃, more preferably 15 ℃, the extraction time is preferably 12 to 48 hours, but is not limited thereto.

The mixed extract or fraction thereof of two or more samples selected from the group consisting of marshmallow, calendula, and St. Mary's plant is as described above with respect to the composition.

The mixed extract of two or more samples selected from the group consisting of the marshmallow, calendula, and Stomatalus of the present invention is effective in improving skin texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation. It exhibits an excellent synergistic effect on the skin, and shows a significant improvement in skin condition compared to the case of using each of them alone.

The weight ratio of two or more selected from the group consisting of the extract of the marshmallow or fraction thereof, the extract of calendula or the fraction thereof, and the extract or fraction thereof of St. Mary's contained in the composition of the present invention is not particularly limited, but two types In the case of a mixed extract, preferably about 1:1, and in the case of a mixed extract of three types, it may be contained in a weight ratio of preferably about 1:1:1. When contained in the above range, there is an advantage in that the skin condition can be effectively improved.

The mixed extract or a fraction thereof of two or more samples selected from the group consisting of the marshmallow, calendula, and Stomatalus of the present invention is a synergistic effect or cooperative action between the extracts and/or the active ingredients in the extract. It shows more excellent skin condition improvement effect than the extract alone, which was first identified in the present invention.

Specifically, the mixed extract of two or more selected from the group consisting of the marshmallow, calendula, and Stomatalus of the present invention or a fraction thereof exhibits excellent skin texture improvement and skin elasticity improvement effect. In addition, the mixed extract of two or more selected from the group consisting of the marshmallow, calendula, and Stomatalus of the present invention or a fraction thereof exhibits excellent anti-inflammatory efficacy. Particularly, the mixed extract of two or more selected from the group consisting of the marshmallow, calendula, and St. mother weed of the present invention, or a fraction thereof, exhibits a more excellent inhibitory effect on nitrogen monoxide (NO) production than the single extract of each plant.

Regarding the above effects, according to a specific embodiment of the present invention, it was confirmed that when the extract or a fraction thereof was treated to a woman, skin texture improvement and elasticity improvement were improved compared to the non-treated comparative example (Table 2).

According to an embodiment of the present invention, an essence containing a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula, and St. Mary's, or a fraction thereof, through these results, is used to prevent or improve skin wrinkles, prevent or improve skin aging. It was found that the effect could also be expressed.

According to another embodiment of the present invention, in order to confirm the collagen synthesis efficacy by treating a mixed extract of two or more samples selected from the group consisting of the marshmallow, calendula, and St. As a result of measuring the content of (Procollagen Type I C-Peptide), by confirming the PICP content similar to the result of treating TGFb1 as a positive control in the mixed extract of two or more samples of the plant extract (Fig. 1), the above of the present invention It was confirmed that a mixed extract of two or more samples or a fraction thereof can promote collagen synthesis through excellent cooperative action.

In addition, according to an embodiment of the present invention, when two or more mixed extracts or fractions thereof selected from the group consisting of marshmallow, calendula, and Stomatalus are treated on mouse macrophages, higher monoxidation than the single extract of the extracts It was confirmed that it could exhibit a more excellent anti-inflammatory effect by exhibiting a nitrogen (NO) production inhibitory effect (Experimental Example 4), and through these results, it was found that the effect of preventing or improving skin aging and improving skin inflammation can also be exhibited.

In addition, the mixed extract of the two or more samples of the present invention or a fraction thereof does not exhibit cytotoxicity in a concentration range that sufficiently shows the effect of improving skin condition, and is derived from a natural product, so it causes serious irritation or harmful effects in the body. It was confirmed that it can be used stably without this (Table 3).

As used herein, the term "improving skin condition" may mean one or more selected from the group consisting of improving skin texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation. .

As used herein, the term "improvement" refers to all actions of at least reducing the severity of a parameter related to the alleviation or treatment of a condition, for example, symptoms.

The cosmetic composition according to the present invention is a solution, external ointment, cream, foam, nutritional lotion, soft lotion, perfume, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil , Suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray can be prepared in a formulation selected from the group consisting of, Not limited to this

In the following quasi-drug composition, food composition, and skin inflammation treatment composition of the present invention, description of two or more mixed extracts or fractions thereof selected from the group consisting of marshmallow, calendula and Stomatalus, improve skin texture, improve skin elasticity, skin wrinkles The description of preventing or improving, preventing or improving skin aging, and improving skin inflammation is the same as described above with respect to the cosmetic composition.

According to another embodiment of the present invention, a quasi-drug composition for improving skin condition containing as an active ingredient a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula and St. Mary's, or a fraction thereof, the improvement of the skin condition Provides a quasi-drug composition that is one or more selected from the group consisting of skin texture improvement, skin elasticity improvement, skin wrinkle prevention or improvement, skin aging prevention or improvement, and skin inflammation improvement.

The term "quasi-drug" as used in the present invention refers to items that are less effective than medicines among items used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals. According to this, quasi-drugs exclude items used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases of humans and animals, and products that have mild or no direct action on the human body.

The quasi-drug composition of the present invention may be prepared as one or more selected from the group consisting of a body cleanser, foam, soap, mask, ointment, cream, lotion, essence, and spray, but is not limited thereto.

According to another embodiment of the present invention, a food composition for improving skin condition containing as an active ingredient a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula and St. Mary's, or a fraction thereof, and improving the skin condition Provides a food composition that is one or more selected from the group consisting of improving skin texture, improving skin elasticity, preventing or improving skin wrinkles, preventing or improving skin aging, and improving skin inflammation.

The food composition of the present invention is not particularly limited, and includes a health functional food composition.

According to another embodiment of the present invention, there is provided a pharmaceutical composition for the treatment of skin inflammation containing two or more mixed extracts or fractions thereof selected from the group consisting of marshmallow, calendula, and St.

The skin inflammatory disease of the present invention refers to all diseases caused by immune stimulation. Non-limiting examples of the skin inflammatory disease include atopic dermatitis, eczema, seborrheic dermatitis, psoriasis, acne, and the like.

In the pharmaceutical composition of the present invention, the effective content of the mixed extract or fraction thereof of two or more samples selected from the group consisting of marshmallow, calendula and St. Mary's is not particularly limited, and 0.00001% to 99.99% by weight based on the total weight of the pharmaceutical composition. It may be included in weight percent or may be included in total content.

The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier in addition to containing a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula, and St. have.

In the present invention, the "pharmaceutically acceptable" means that it can be used conventionally in the field of pharmacy, which does not stimulate an organism when administered, and does not inhibit the biological activity and properties of the administered compound.

The pharmaceutical composition of the present invention may be formulated with the carrier and used as food, medicine, feed additive, drinking water additive, and the like.

The pharmaceutical composition of the present invention may be formulated and used in various formulations suitable for oral administration or parenteral administration.

A preferred dosage of the pharmaceutical composition of the present invention may be 1 mg/kg to 1,OOO mg/kg per day.

The route of administration and the method of administration of the pharmaceutical composition of the present invention may each be independent, and the method is not particularly limited, and any route of administration and administration method may be applied as long as the pharmaceutical composition can reach the desired site. You can follow.

The pharmaceutical composition may be administered orally or parenterally. As the parenteral administration method, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, etc. can be used, and a method of applying, spraying, or inhaling the composition to a diseased site may also be used. Can be, but is not limited to these.

The pharmaceutical composition of the present invention may be preferably administered parenterally, more

Preferably, it may be administered by topically applying to a region in need of improving skin conditions or preventing, improving or treating skin diseases.

The term "treatment" used in the present invention refers to the pharmaceutical composition of the present invention.

It refers to any action that is administered to the skin to improve the symptoms of inflammatory diseases or to benefit.

According to another embodiment of the present invention, comprising administering to an individual a cosmetic composition, quasi-drug composition, or food composition containing two or more mixed extracts or fractions thereof selected from the group consisting of marshmallow, calendula and St. Provides a method of improving the skin condition

In the present invention, the term "individual" means all animals, including mammals, including mice, livestock, humans, and the like.

In the present invention, description of the dosage, route of administration, administration mode, etc. of the composition

Is the same as described in connection with the pharmaceutical composition of the present invention.

The composition containing a mixed extract of two or more samples selected from the group consisting of marshmallow, calendula, and St. Mary's plant of the present invention or a fraction thereof as an active ingredient is excellent in skin regeneration, skin elasticity effect, and skin wrinkles, skin aging, skin It is also excellent in preventing, improving or treating inflammation and skin pigmentation. Specifically, the composition of the present invention is excellent in the effect of increasing the total amount of collagen and suppressing the inflammatory reaction.

In addition, the composition of the present invention is a material derived from natural products, so it is not toxic,

Even if applied, it does not cause side effects, so it is highly applicable to cosmetics, quasi-drugs, food and/or pharmaceutical compositions.

Figure 1 shows the collagen production effect of the plant mixture extract according to an embodiment of the present invention (negative control; purified water, positive control; TGF-b1, PIP; Procollagen Type 1 C-peptide).

Hereinafter, the present invention will be described in more detail by the following examples and experimental examples. However, the following Examples and Experimental Examples are for illustrative purposes only, and the scope of the present invention is not limited by the following Examples and Experimental Examples.

Manufacturing example 1: Preparation of plant extract

In the case of using plant extracts alone, in order to solve the insignificant effect on skin improvement, that is, to obtain an extract of cooperative action rather than flavonoids from ordinary plants alone, extracts of each plant without destroying the effective ingredients by vacuum low temperature method. In addition to flavonoids, an extract from which flavonoids capable of cooperative action were extracted was prepared.

Specifically, the roots of marshmallows, flowers of calendula, and leaves of Stomatalus were washed clean with water, dried well in the shade, and then pulverized. After the pulverized product was added to an extraction container, extraction was repeatedly performed under vacuum at a low temperature of 15° C. with ethanol to obtain an extract. The obtained extract was filtered to remove solids using filter paper, etc., and the extract was concentrated under reduced pressure to prepare an extract.

Example 1 to 5 and Comparative example : Vacuum low temperature Preparation of Essence Composition Containing Plant Extracts Prepared Through Method

In order to check the effect of improving the skin condition of the plant extract prepared according to Preparation Example 1, a cosmetic composition of an essence formulation was prepared as shown in Table 1 below (unit: wt%). Specifically, extracts of the marshmallow roots, calendula flowers, and the leaves of St. Mary's were prepared and then mixed to prepare a mixed extract.

Raw material name Comparative example Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Cetearyl alcohol 2 2 2 2 2 2 2 Cetearyl glucoside One One One One One One One Jojoba seed oil 5 5 5 5 5 5 5 Silica 0.5 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 10 10 10 10 10 10 10 Marshmallow extract - 2.5 - - 2.5 - 2.5 Calendula extract - - 2.5 - 2.5 2.5 2.5 St. Mary's Extract - - - 2.5 - 2.5 2.5 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100

Experimental example 1: Measurement of skin improvement effect

For the products of Comparative Examples and Examples 1 to 6, the skin improvement effect was performed as follows for healthy women aged 30 to 50 years.

Essences of Examples 1 to 6 containing 5% by weight of a single extract or a mixture of two or more extracts of marshmallow, calendula, and St. Mary's in the right eye area for 35 women aged 30 to 50 years old. Was applied twice a day for 3 months.

After 3 months, the degree of skin improvement was evaluated through a questionnaire of subjects and image analysis of the skin. The subject's questionnaire was judged as three stages of improvement in skin texture and elasticity: no improvement, slight improvement, and significant improvement compared to before use, and the results are shown in Table 2 (unit: persons).

sample No improvement (persons) Slight improvement (persons) Significant improvement (persons) Comparative example 8 2 0 Example 1 One 4 5 Example 2 0 6 4 Example 3 6 One 3 Example 4 One 5 4 Example 5 0 6 4 Example 6 One 4 5

As shown in Table 2, when Examples 1 to 6 according to the present invention were used, it was confirmed that skin texture improvement and elasticity improvement were improved by 45% to 50% compared to the comparative example, and accordingly, the example product of the present invention It was found that this is effective for skin improvement.

Experimental example 2: Collagen synthesis test

In order to evaluate the effectiveness as a cosmetic material, marshmallow, calendula, and St. mother weed extracts and a mixture of two or more extracts thereof were added to the culture medium of human-derived fibroblasts to test the effect of collagen production at the cellular level.

The measurement of the total amount of increased collagen was quantified using PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT) (FIG. 1).

After each test sample was added to the culture medium of human fibroblasts to be 1% and cultured, the culture solution was taken and the degree of increase in the total amount of collagen at each concentration was measured using a spectrophotometer (450 nm) with PICP EIA kit. .

In order to compare the effects, purified water as a negative control group and TGF-b1 (10 mg/ml) as a positive control were treated in the same manner as described above to the culture medium of fibroblast cells to measure the PIP content.

As shown in FIG. 1, the PIP content was 128 mg/ml in the negative control group, 211 mg/ml in the positive control group, and in the case of marshmallow, calendula, and St. ml was higher than that of the negative control group. In addition, in the case of two or more mixtures of marshmallow and calendula, calendula and St. Mary, marshmallow, calendula, and St. It was higher than that of the single extract.

Therefore, it was confirmed that the mixed extracts of two or more types treated with the same concentration than each of the individual plant extracts were effective in synthesizing collagen through mutually superior cooperative action.

Experimental example 3: Cytotoxicity test

To verify the primary safety as a raw material used in cosmetics, V79-4 cells (continuous cell lines of Chinese hamster, lung tissue fibroblasts) were cultured for the marshmallow, calendula, and St. Test [Reference: Mossman T. (1983). Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55-63] was performed.

Specifically, V79-4 cells were cultured in DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 10% fetal bovine serum for 24 hours, and then replaced with serum-free DMEM. Test samples (Comparative Examples, Examples 1 to 5) were added to the culture medium and incubated in an incubator at 37° C. and 5% CO ₂ for 3 days, and then the supernatant was removed and washed with PBS (phosphate buffered saline). After adding 1.0 ml of the solution per well, the MTT solution was removed after 4 hours, and 1.0 ml of DMSO was added per well to dissolve formazan, and then ^a IC ₅₀ The values were measured and shown in Table 3 below. The ^a IC ₅₀ refers to a concentration that kills 50% of cells at 50 Inhibitory Concentration.

sample IC ₅₀ (%, w/v) Sodium lauryl sulfate 0.005 Squalane 0.05 Marshmallow extract IC ₅₀ =1 Calendula extract IC ₅₀ =1 St. Mary's Extract IC ₅₀ =1 Marshmallow, Calendula extract IC ₅₀ =1 Calendula, St. Mary's Extract IC ₅₀ =1 Marshmallow, Calendula, St. Mary's Herb Extract IC ₅₀ =1

As shown in Table 3, the IC ₅₀ value of the marshmallow, calendula, and Stomatalus extract of the present invention and a mixture of two or more extracts thereof is 1.0, 2000 times or more than sodium lauryl sulfate (0.005), a useful ingredient with excellent safety. The cytotoxicity was 200 times lower than that of squalane (0.05).

As a result of such an experiment, it was found that the safety of the single extract and the mixed extract of the present invention is very excellent and can be used as a cosmetic ingredient.

Experimental example 4: Anti-inflammatory effect test ( Nitric Oxide assay )

In order to confirm the anti-inflammatory effect of the marshmallow, calendula, and St. mother weed extracts of the present invention and a mixture of two or more extracts thereof, a nitrogen monoxide production inhibitory effect was conducted using the Raw 264.7 cell line.

Specifically, the effect of inhibiting the production of nitric oxide (NO) was evaluated by the single extract of marshmallow, calendula, and St. Mary's plant at the same concentration and a mixture of two or more extracts thereof using Raw 264.7 cells, which are macrophages of mice.

The test sample of the present invention was diluted to 1% concentration and treated in a culture medium 30 minutes before. At this time, for comparison of the effects, 10 mg/ml L-NG-Monomethylarginine (L-NMMA), a nitrogen monoxide production inhibitor, was treated as a positive control in the same manner as the samples.

The culture medium was treated with 1 mg/ml of lipopolysaccharide (LPS) as a stimulating source and cultured for 24 hours, and then the ability to inhibit nitrogen monoxide production was evaluated using a nitrogen monoxide quantitative kit.

NO production inhibitory ability (%) = {1-(nitrogen monoxide production when sample is added/nitrogen monoxide production without sample)} x 100

Sample (1%) Nitrogen monoxide production inhibition ability (%) Positive control 42 marshmallow 22 Calendula 18 Our Lady 15 Marshmallow, Calendula 35 Calendula, Our Lady 31 Marshmallow, Calendula, Virgin Mary 44

As shown in Table 4, the ability to inhibit nitrogen monoxide production was 42% in the positive control group, and 22%, 18%, and 15% in the case of marshmallow, calendula, and St. On the other hand, in the case of two or more mixtures treated with the same concentration as the single extract, marshmallow and calendula, calendula and St. Mary, marshmallow, calendula, and St. It was confirmed that the above mixed extract had a higher ability to inhibit nitrogen monoxide production than the single extract. In addition, it was found that in the case of the three mixtures (marshmallow, calendula, and Stomatalus), the ability to inhibit nitrogen monoxide production was higher than that of the positive control group and the two mixtures.

Through these experimental results, two or more mixtures treated with the same concentration than each of the single extracts have an effect of inhibiting the production of nitrogen monoxide similar to that of the positive control group (L-NMMA) through excellent cooperative action. And it was found.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof, are included in the scope of the present invention.

Claims (9)

A cosmetic composition for improving skin wrinkles or elasticity, comprising as an active ingredient an extract of at least one sample selected from the group consisting of marshmallow and St.
The cosmetic composition of claim 1, wherein the composition further comprises a calendula extract or a fraction thereof.
The cosmetic composition according to claim 1, wherein the extract or its fraction is contained in an amount of 1 to 30% by weight based on the total composition.
The cosmetic composition of claim 1, wherein the skin wrinkle or elasticity improvement is performed by promoting the synthesis of collagen.
The cosmetic composition according to claim 1, wherein the composition is made of cosmetics, body care products, and hair care products.
A quasi-drug composition for improving skin wrinkles or elasticity, containing as an active ingredient an extract of one or more samples selected from the group consisting of marshmallow and St.
The quasi-drug composition of claim 6, wherein the composition further comprises a calendula extract or a fraction thereof.
A food composition for improving skin wrinkles or elasticity, comprising an extract of at least one sample selected from the group consisting of marshmallow and St. Mary's plant or a fraction thereof as an active ingredient.
The food composition of claim 8, wherein the composition further comprises a calendula extract or a fraction thereof.

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