KR20230077352A - Cosmetic composition containing sodium hyaluronate - Google Patents

Abstract

The present invention is a cosmetic essence composition for improving skin wrinkles composed of collagen, sodium hyaluronate, adenosine, lysine, and purified water, and succinic anhydride added to a solution dissolved by adding atelocollagen sand to an acetic acid solution, and caustic soda When it is completely dissolved in the state of maintaining pH 10 with hydrochloric acid, adjusting to pH 4.03, centrifuging to obtain sand, and obtaining atherocollagen treated with succinic acid by-product frozen and vacuum-dried;
Aterocollagen, adenosine, and purified water treated with the succinic acid by-product were added to a solution of 0.5% hyaluronic acid and first dissolved in a state maintained at 5 ° C or less, and caustic soda (0.5N) was added to maintain pH 6.1 After completely dissolving the undissolved collagen in the state, a small amount of lysine was added and dissolved, and then supplemented with evaporated purified water and adjusted to pH 6.1 with caustic soda (a5N). it's about

Description

Cosmetic composition containing sodium hyaluronate {Cosmetic composition containing sodium hyaluronate}

The present invention relates to a cosmetic essence composition using succinoyl atelo collagen, adenosine, lysine, and sodium hyaluronic acid, which are active ingredients for improving skin wrinkles, and a manufacturing method thereof

Recently, as interest in anti-aging has increased, collagen has been greatly highlighted. Many products are pouring out, including cosmetics to prevent wrinkles, anti-aging foods, and even diet foods.

Regarding the collagen we eat, for example, there is no scientific evidence that eating collagen has increased the amount of collagen in our body. Collagen itself is decomposed in the body and absorbed into amino acids as soon as it is eaten, so it does not fit the basic logic that eating collagen increases the amount of collagen, eliminating wrinkles and improving skin. Also, speaking of collagen applied to the skin, the collagen molecules are too large to penetrate the skin. In other words, if the size of molecules that can pass through skin tissue is 500, the size of collagen molecules is 600 times that of 300,000, so it can be said that it is impossible to pass through skin tissue. Therefore, although it can only provide a moisturizing effect to the skin, it is said that there is no effect of penetrating into the skin tissue and filling the insufficient collagen to smooth out wrinkles and make the skin elastic.

Collagen, which is said to be effective in improving skin wrinkles, can be largely divided into animal collagen, pseudo-collagen, and marine collagen. It is collagen extracted from the bones or skins of animals, and similar collagen is prepared similarly to collagen by modifying vegetable polysaccharide (natto gum) by enzyme fermentation. Marine collagen is collagen extracted from marine sources such as various fish and starfish.

Pseudo-collagen mainly has the ability to hold and bind a lot of water, so it is very helpful for skin hydration, and animal collagen and marine collagen are known to have not only a moisturizing function but also a wrinkle-improving function.

So far, animal collagen extracted from animals such as cows and pigs has been mainly used, but recently, marine collagen extracted from the sea has been used a lot.

Marine Collagen has 4 times higher serine content than other collagens and is low-molecularized through enzyme treatment, so it is quickly absorbed in the body and is convenient to use as a liquid. In addition, this collagen supplies the characteristics of existing collagen as it is, is safe for skin and hair, and has the ability to absorb moisture in the air, so it plays a role in maintaining moisture at all times.

However, since marine collagen is used after enzymatic hydrolysis to have an average molecular weight of about 1000, it cannot be called true collagen. The molecular weight of pure collagen is about 300,000, but the molecular weight of marine collagen is too small compared to this. This may mean that marine collagen is not pure collagen, but only gelatin in which collagen is decomposed. Gelatin can supply moisture to the skin temporarily, but it cannot expect the effect of wrinkle improvement.

Therefore, as mentioned above, marine collagen has recently been attracting attention and being used a lot, but it is only gelatin that cannot function as collagen is broken down, so it is ultimately very different from collagen extracted from animals. So, marine collagen and other vegetable collagens can be stored and handled at room temperature, but collagen products extracted from animals must be refrigerated to prevent denaturation of collagen into gelatin.

In order to truly improve skin wrinkles, I think that products made from undenatured collagen extracted from animals should be used.

As described above, collagen is mainly used in food and cosmetics, but recently, many research institutes around the world, mainly in the United States, are researching artificial organs using collagen. Institutions, etc. are participating in artificial organ research and several venture companies are releasing research results as products, and this is also producing high-purity medical collagen that can make artificial skin at Dalim Tissen, which is an applicant, and artificial skin is also researched. are among

Among the development of artificial organs, artificial skin was the first product to be introduced into the market.

Currently, artificial skin uses a method of removing cells from the patient's body, promoting tissue formation with a collagen matrix and endocrines such as growth factors, making them into the required size and shape, and then transplanting them to the required area. It is said that the technology of culturing cells to create an artificial organ is highly relevant to the extent that companies developing artificial skin sometimes develop artificial cartilage as well.

The range of uses of artificial skin is very diverse, and it can be used for burns, bedsores, diabetic ulcers, intractable ulcers, cartilage damage, trauma patients, amputation patients, and plastic surgery patients. The main target is burn treatment, and it is expected that patients with severe burns who have suffered from no other treatment so far can benefit the most, but the field that will actually form a large market is patients with pressure sores (including diabetic ulcers) and skin plastic surgery. It is thought to be a market for As diabetes continues to increase due to an aging society and a westernized diet, the potential market size of artificial skin is expected to be very large.

In the future, if only a few more problems are solved, such as the low clinical results due to insufficient clinical trials, the difficulty of mass production and the high price due to collagen imports, and the fact that cell culture does not work well for the elderly. The ripple effect of artificial skin is thought to be enormous. Dalim Tissen believes that by directly producing high-purity medical collagen, it will have a great advantage in terms of price when developing artificial skin.

Although the comprehensive use of collagen has been mentioned above, looking at the specific prior art used in cosmetics, skin care (functional) cosmetics in the United States are currently the most popular functional cosmetics, which are ranked first in sales. It is a nutrient lump composed of 29 kinds of synthetic Vyo-Celum, including protein, and is known as a cosmetic product with great wrinkle improvement, skin elasticity increase, and whitening effect.

However, when looking at the ingredients of this product, it was found that about 22 substances were included, and half of them, 10 to 11, were preservatives. Most of them are paraben-type preservatives, which are representative preservatives used in cosmetics and have recently been found in breast tumor samples such as breast cancer and are problematic. If you go to the OrganicConsumers Association site, there is an article titled “Beware of Paraben Preservatives in Body Care Products. Parabens are known to have adverse effects on the body, and parabens flow into the blood through the skin. In addition to parabens, this product also contains an auxiliary preservative for parabens called imidazolidinyl urea, which is a preservative for sensitive areas. It is a preservative that is not good enough to not be used in products applied around the eyes (eye cream, etc.) due to irritation.

In addition, the collagen contained in the cosmetics is hydrolyzed dried collagen, and collagen cut due to enzyme treatment is called collagen peptide, which is a material in which collagen is cut, so it is a different material from collagen and therefore cannot properly function. Although peptides have important physiological activities, they have not been known yet.

As another prior art, Korean Patent Publication (Publication No. 10-2005-0039923) introduces a cosmetic additive using titanium dioxide and a collagen compound and a manufacturing method thereof. The composition of the technology is titanium dioxide 1-20%, The first solution mixed with 80 to 99% of oil and dissolved while stirring at 60 to 70 ° C. and the second solution mixed with 10 to 15% of collagen and 85 to 90% of distilled water and dissolved while stirring at 60 to 70 ° C were 1: As a cosmetic additive formulated in No. 1, titanium dioxide is soluble in concentrated inorganic acid solution, but does not react or dissolve with oil, and is mixed with collagen in distilled water. can't exert

In addition, Korean Patent Publication (Publication No. 10-2004-0042981) provides collagen that can increase the temperature range in which collagen is thermally denatured as a cosmetic raw material containing collagen, thereby increasing the denaturation temperature of collagen for storage and distribution. Technical details that can lengthen the quality maintenance period are introduced, and in the Korean Patent Publication (Publication No. 10-2007-0027634), a cosmetic composition containing a protein hydrolyzate, in detail, the protein hydrolyzate is terminal proline It relates to a cosmetic composition having a large amount of peptides, but none of the above means or methods for improving penetration into the skin layer can be found.

The present invention is a cosmetic essence composition for improving skin wrinkles composed of collagen, adenosine, lysine, and hyaluronic acid distilled water, and in its preparation, does not cause an immune response, has excellent penetration into the skin, and prevents the propagation of microorganisms during use It is to provide a cosmetic essence composition for improving skin wrinkles and a manufacturing method in which the cosmetic essence composition is treated with a weak acid in order to increase the skin absorption rate by having an exfoliation function as soon as the cosmetic is applied.

Natural collagen is treated with an enzyme (pepsin) to remove the telo-peptide present on both ends of collagen that causes an immune response, and then treated with succinic anhydride to improve the collagen surface. is electrically transformed into an anionic state so that the aged cell membrane takes on a positive (+) charge property, greatly improving the permeability into the epidermis through electrostatic interaction with the skin, and at the same time treating the composition in a weakly acidic state to treat microorganisms and bacteria It is possible to provide a cosmetic essence composition for improving skin wrinkles and a manufacturing method according to the present invention by preventing the propagation of the skin, easily removing dead skin cells and increasing skin absorption rate.

The cosmetic essence composition and its manufacturing method according to the present invention use attello collagen, so that when the cosmetic essence composition penetrates the skin, it does not cause an immune response, and treats the attelo collagen with succinic anhydride, so that the collagen surface is electrically anionic. It greatly improves permeability into the epidermis through electrostatic interaction with aged cell membranes, which are positively charged, and also prevents the propagation of microorganisms and bacteria through weak acidification of collagen, and can easily remove dead skin cells when applied to the skin. It can increase skin absorption.

In addition, the collagen membrane containing hyaluronic acid promotes the proliferation of fibers and cells and increases the migration ability of cells, and adenosine promotes the DNA of skin fibers and cells, increases the synthesis of protein, and increases the size of cells. It activates protein metabolism or helps to maintain anti-tension of skin cells where function decline occurs, improves cell function, expands cell size, and has the effect of improving wrinkles due to destruction and aging of dermal cells. It is a substance that is not synthesized and is absorbed into the body through foods such as beans. It has the function of repairing growth tissues and prevents the proliferation of the herpes virus, so the function of inhibiting virus growth is involved in collagen formation, suppressing muscle loss, and reducing muscle loss caused by post-surgery and trauma. It can be said to be a cosmetic essence composition having an excellent skin wrinkle improvement effect by having a function of quickly recovering from tissue damage and having a synergistic effect with a mutually complementary function between the compositions.

1 and 2 is a schematic perspective view for explaining a cosmetic made of a cosmetic composition containing sodium hyaluronate.

The cosmetic essence composition for improving skin wrinkles according to the present invention is a composition composed of collagen 0.4 ~ 4wt%, hyaluronic acid 0.4 ~ 4wt%, adenosine 0.04 ~ 0.4wt%, lysine 0.0002 ~ 0.002wt%, the rest being purified water 91.6 ~ 99.15wt% can do.

The collagen used above is treated with succinic anhydride to remove the telo-peptide present on both ends of the collagen by treating natural collagen with an enzyme (pepsin). Collagen electrically transformed into an anionic state is used.

In addition, the hyaluronic acid used in the above is a composition ratio set based on 100% hyaluronic acid as a 0.5% aqueous solution is used in the actual composition. In addition, liposomalized adenosine is used for adenosine to prevent precipitation.

Looking at the function and action of each composition used in the cosmetic essence composition, collagen and hyaluronic acid are widely known as extracellular matrix substances with many biological functions. is a factor that affects Clot formation occurs when circulating platelets attach to collagen exposed on the blood vessel wall due to injury. Hyaluronic acid is a unique non-sulfated polysaccharide that exists in an unbound state and is present in all living organisms and plays an important role in various major functions such as substrate structure, water balance, lubrication, cell migration and differentiation. [Turley EA,.et al,.J Biol Chem 2002277:4589-92], because of these biological properties, hyaluronic acid has been used for tissue engineering applications as a biosupport material along with other extracellular matrix components such as collagen. [Orgill DP, et al,. J Biomed Mater Res 199839:531-5; Miralles G. et al., J Biomed Mater Res200157:267-78], Yonsei University School of Medicine, Medical Engineering Department, in an experiment, a collagen membrane containing 9.6% hyaluronic acid was compared with a polyurethane membrane or a cell culture dish surface. It was observed that the proliferation of fibroblasts was promoted when fibroblasts were stimulated. In addition, it was observed that the migration ability of cells increased in the matrix containing 5.4% or 9.6% hyaluronic acid. [ParkSN, et al., Biomaterial 2003:24:1631-41], therefore, it appears that the main reason for preparing a matrix containing collagen and hyaluronic acid is their structural and functional role in wound healing.

Collagen is a structural basic component of the skin, and as aging progresses, the amount decreases and the ratio between type 1 and type 3 changes. In particular, sunlight, smoking, pollutants, etc. increase collagenase, an enzyme that decomposes collagen, and accelerate skin aging. Therefore, collagen-based fillers for tissue repair can replace dermal structures reduced by aging and are still the standard for all other dermal graft materials.

When collagen is added to cosmetics or foods, the form of collagen must be slightly modified in order to be well absorbed by the skin or to remove the part that causes an immune response.

Collagen used in the present invention is collagen that is modified so that the surface of atherocollagen is electrically anionic by treating native collagen with an enzyme (pepsin) to remove telo-petide present on both ends of collagen. can be said

By allowing the formation of negative ions in collagen, collagen that dissolves in acidic solutions can dissolve in neutral solutions, which is a very important part when making cosmetics. Because cosmetics are applied to the human body, their acidity should be close to neutral. Therefore, anion-formed collagen has the advantage that it can be produced even in a neutral solution.

It also has the advantage of increasing penetration into the skin by forming an ionic bond with the skin.

Adenosine, along with retinol, retinyl palmitate, and polyethoxylated retinamide, is a wrinkle improvement notice ingredient from the Food and Drug Administration. This substance is an essential component of APT for the activity of living things, and is easily absorbed into cells and synthesized in the body. It is an important substance that regulates various metabolisms. has been

Adenosine promotes DNA synthesis of skin fibroblasts, increases protein synthesis, and serves to increase cell size. Increases nucleic acid synthesis and activates protein metabolism to help maintain anti-tension of dead or degraded skin cells, improve cell function, and expand cell size to improve wrinkles due to destruction and aging of dermal cells It also gives vitality to the skin and enhances skin elasticity. It also has the advantage of high safety and plays a role in promoting collagen synthesis.

In the present invention, not only collagen but also adenosine are included, so it has a synergistic effect on wrinkle improvement. In addition to the high absorption of collagen into the skin, the function of adenosine is added to improve wrinkles more effectively.

▶ General properties of adenosine

* Stability: Since adenosine has a structure that does not have a specific double bond and is relatively stable against low temperatures, it is a substance that does not require an expiration date for functional cosmetics. However, since it has a sugar attached to adenine, it is very vulnerable to bases and partially vulnerable to heat, so it is a substance that needs stabilization to preserve long-term potency.

* Skin permeability: The structure of adenosine is a structure in which adenine is attached to a sugar, so it is a substance with low skin permeability because it is water-soluble. In particular, since the functional effect value set by the Korea Food and Drug Administration is very low at 0.04%, reaching the dermal layer through skin permeation is a prerequisite for expecting the efficacy of adenosine. Therefore, it can be said that it is essential to increase skin permeability in order to obtain a desired degree of wrinkle improvement effect at a low concentration.

* Convenience of manufacturing (simplification):

* Precipitation Prevention: Although Adenosine is a hydrophilic substance, it is a substance that exhibits very unique characteristics in terms of solubility. As a substance with high possibility of precipitation with time in the formulation, the problem of precipitation can be solved by using liposomalized adenosine.

* Completion of CTFA registration: Domestic cosmetic raw material permits require the use of only CTFA-listed raw materials or those approved for longevity. Unregistered raw materials can in fact be considered illegal raw materials.

▶ Efficacy / Effect

Skin elasticity improvement and skin wrinkle prevention effect

* Promote fibroblast proliferation

* Increase collagen synthesis

* Inhibition of collagenase activity

* Transduction of skin cell activation signal in the dermis

* Increased size of fibroblasts

- lysine

It plays an important role in maintaining the body's nitrogen balance. It is a kind of essential amino acid and cannot be synthesized in the body, so it must be consumed through food or supplements. In particular, lysine is classified as a limiting amino acid among essential amino acids because its food content is very low.

It is abundant in animal foods, but lacking in foods other than soybeans, so Koreans, whose staple food is rice, are prone to lysine deficiency, and especially those who do not eat soybeans are at greater risk of deficiency.

The function of lysine in the body is

First, it is an essential ingredient for the recovery of growth tissues and all protein components such as enzymes and hormones. It is directly involved in the growth of children and the creation of bones, and most of the growth factors in the body require lysine.

Second, it has the effect of preventing and improving osteoporosis. Lysine is needed to transport minerals to the bone.

Thirdly, lysine inhibits the growth of viruses such as the common cold or herpes simplex because it inhibits the growth of the herpes virus.

Fourth, it lowers triglycerides to prevent heart disease. Along with other amino acids, proline and vitamin C, lysine prevents coronary artery disease by inhibiting the formation of arterial blood clots in lipoproteins.

Fifth, it is involved in the production of antibodies, enzymes, hormones and collagen formation, thereby inhibiting muscle loss and quickly recovering tissue damage caused by surgery and trauma. There are many other functions, and when these lysine is deficient, symptoms such as anorexia, anemia, fatigue, decreased concentration, congestion of the eyes, nausea, enzyme imbalance, poor growth, hair loss, and weight loss may appear.

As such, lysine is usefully used in various fields. It is used as a recovery accelerator for the health recovery of the elderly and the infirm, and is also used as an effect enhancer added to medicines to increase breastfeeding of mothers and drinks to relieve physical fatigue after exercise.

Collagen is a representative material of hyaluronic acid having functions of improving wrinkles and preventing aging, and another representative material related to this collagen is hyaluronic acid.

Hyaluronic acid is a representative material constituting connective tissue together with collagen and chondroitin sulfate.

This is a substance similar to collagen, which plays an important role in maintaining skin elasticity by containing dozens of times more moisture than the actual volume. And its large molecular structure makes the dermis more flexible and forms an elastic film when applied to the skin. When this component is reduced, moisture is rapidly reduced and the elasticity of the skin decreases, resulting in wrinkles.

Most of the filler procedures used in dermatology and plastic surgery to treat deep wrinkles such as nasolabial folds use supplements such as Restylane and Juvederm made from hyaluronic acid.

In the present invention, not only collagen and adenosine but also hyaluronic acid are included, so there is an excellent effect on wrinkle improvement.

Hyaluronic acid is a polysaccharide having a molecular weight of 50,000 to 10,000,000 Da or more as a linear polymer in which B-D-N-acetylglucosamine and B-D-glucuronic acid are alternately bonded. Hyaluronic acid is a basic substance of biological connective tissue, mainly mammalian skin, synovial fluid of joints, vitreous humor of eyes, umbilical cord, serum, and cock's comb. It is distributed on the back and is also known to exist in the capsules of streptococci and bacilli. Common methods for obtaining hyaluronic acid include extracting from chicken combs, umbilical cords, etc., culturing streptococci and bacilli, and then extracting and refining them.

Although natural hyaluronic acid is polydisperse in proportion to its molecular weight, it is known to exhibit excellent biocompatibility when transplanted or injected into a living body regardless of its origin because it has no interspecies specificity and no tissue or organ specificity. However, when hyaluronic acid is applied to the body as it is, it is easily decomposed by an enzyme called hyaluronic acid present in the body, so the half-life in the living tissue is about 0.5 to 3 days, so the residence time in the body is relatively short, so it is used as a medical material. There are limits to what you can do. In order to expand its use to medical materials, attempts have been made to improve the durability of hyaluronic acid in the body by cross-linking it using various chemical modifiers or modifying it by attaching functional groups. come.

Among the attempts to modify the physicochemical properties of hyaluronic acid to use it for various purposes, in particular, porous sponges manufactured by various methods are porous supports or upper body covering materials for the purpose of culturing and transplanting cells or tissues. , medical materials such as dental matrices, and pharmaceutical materials such as carriers of drug delivery systems.

Hyaluronic acid is a major component of the dermal connective tissue matrix and exists in the form of a viscoelastic liquid that surrounds collagen and elastin fibers. Unlike collagen, the chemical structure of all species is the same, so it does not cause an immune response. The hydrophilicity of hyaluronic acid attracts and retains water in the extracellular matrix space, affecting tissue volume and pressure. Aged skin In the case of hyaluronic acid, the amount of hyaluronic acid is reduced and moisture is lost accordingly, eventually decomposing collagen and elastin fibers, which are characteristics of aged skin.

The use of hyaluronic acid as a tissue restoration material is ideal, but the maintenance period in its natural structure is about 1 to 2 days. Therefore, to increase the durability, a cross-linking method is used. Cross-linked hyaluronic acid, called hylan or hyaluronic acid gel, swells in water, maintains viscosity, and can pass through small needles.

Another characteristic of hyaluronic acid gel is its anti-bulk degradation properties. As each molecule of hyaluronic acid is decomposed, the remaining molecules retain more moisture, so the total amount of moisture remains unchanged.

As an example, the manufacturing method (composition method) of the cosmetic essence composition of the present invention as the raw material as described above

28.000 g of 0.5% hyaluronic acid (140 g of hyaluronic acid based on 100% purity), 140 g of succinylated atelo collagen treated with succinic anhydride, 14 g of adenosine, and 6,846 g of purified water were added to keep the temperature below 5 ° C. 0.5N NaOH was added to dissolve poorly soluble collagen, and stirred until collagen was completely dissolved while maintaining pH 6.1, then 0.07 g of lysine was added, stirred again, and the evaporated water was converted to purified water. At the same time as replenishment, a cosmetic essence composition for improving skin wrinkles can be prepared by adjusting again to 6.1 with 0.5N NaOH.

Prior to the manufacturing method (composition method), succinic anhydride treatment of atherocollagen is performed by placing 150 to 180 g of atelocollagen needles in 5 liters of 0.1M HAc (acetic acid), stirring, and dissolving to obtain succinic anhydride. 9.5g was added and stirred for 2 hours while maintaining pH 10 using NaOH. When the succinic anhydride was completely dissolved, stir for 10 to 20 minutes while maintaining pH 4.03 using HCI,

The colloidal solution was separated by a centrifugal separator, and only sediment was separated, adjusted to pH 4.03 with 0.2 L of purified water, stirred again for 15 to 20 minutes, and centrifuged a second time to obtain sediment. This is a method of obtaining atherocollagen treated with succinic anhydride by spreading the seam sand thinly on a flat plate, freezing it, vacuum-drying it at -40 ° C, and vacuum-packing it.

Through the above succination treatment process, the collagen surface can be electrically transformed into an anionic state.

This was able to improve the permeability of collagen into the epidermis by electrostatic interaction with the skin by anionizing collagen, focusing on the increase in the positive (+) charge property of aged skin cell membranes.

In addition, in the above process, the weak acidification of collagen can prevent deterioration by preventing the propagation of microorganisms and bacteria, and when applied to the skin, it is easy to remove dead skin cells caused by aging to improve skin absorption rate, and lysine also has a virus growth inhibitory function, making it a cosmetic product. A synergistic effect is obtained in preventing deterioration of the essence composition.

In addition, the composition ratio in the mutual relationship of the composition first causes a problem in viscous solubility in the relationship between total solid content and constant jae number, and also in workability and commercial phase. For example, when a lot of collagen is used, it does not dissolve well and takes a long time in the composition process, and adenosine has low solubility and tends to precipitate in cosmetic compositions, so it cannot be used in large amounts. In the case of hyaluronic acid, it is difficult to adjust the pH of the composition when a large amount is used, and when used in a small amount, the proliferation function of fibroblasts along with collagen decreases, and the structure of the matrix, water balance, lubrication, cell migration function, and bacterial invasion prevention function it falls

In particular, the relationship between collagen and hyaluronic acid is inseparable, and cell migration and proliferation of fibroblasts are activated only when they are composed in an appropriate ratio. The composition ratio of mutual relationship can be said to be a composition ratio established by tests, experiences, and related literature over many years.

Summarizing the characteristics of the cosmetic essence composition for improving skin wrinkles and its manufacturing method according to the present invention, atherocollagen is used to reduce the immune response, and atherocollagen is treated with succinic acid anhydride to negatively charge collagen to form positively charged properties. The band improves the permeability of collagen into the epidermis by electrostatic interaction with the aged cell membrane, and considering the characteristics of adenosine, which is weak to bases and heat, reacts in a cold state and maintains a weakly acidic state, and liposomes to prevent precipitation in the essence composition in solution Stabilization is achieved by using purified adenosine, and by maintaining the composition in a slightly acidic state, it is possible to prevent the propagation of microorganisms and bacteria, and it is possible to easily remove dead skin cells from aged skin, thereby increasing the skin absorption rate.

Claims (4)

A cosmetic essence composition for improving skin wrinkles composed of collagen 0.4-4wt%, hyaluronic acid 0.4-4wt%, adenosine 0.04-0.4wt%, lysine 0.0002-0.002wt%, and purified water 91.6-99.15wt% in weight ratio Adding atelocollagen treated with succinic anhydride and adenosine purified water to a 0.5% hyaluronic acid solution, stirring while maintaining at 5 ° C or less to dissolve the first step;
completely dissolving the undissolved collagen while maintaining the solution at pH 6.1 with 0.5% caustic soda; and
Stirring and dissolving by adding 0.07 g of lysine to the solution in which the collagen was completely dissolved;
A method for producing a cosmetic essence composition for improving skin wrinkles, comprising the steps of replenishing the water evaporated from the solution with purified water and readjusting it to pH 6.1. The succinylated atelocollagen according to claim 2 is dissolved by adding atelocollagen needles to 0.1M acetic acid solution and stirring, then adding succinic anhydride and stirring for 2 hours while maintaining pH 10 with caustic soda to completely dissolve succinic anhydride. When the pH is adjusted to pH 4.03 using hydrochloric acid and stirred to obtain a colloidal solution, the colloidal solution is centrifuged and only the sediment is separated and adjusted to pH 4.03 with purified water. A method for producing a cosmetic essence composition for improving skin wrinkles, characterized in that it is atherocollagen treated with succinic anhydride vacuum-dried at ° C. The method for preparing a cosmetic essence composition for improving skin wrinkles according to claim 2, wherein adenosine is liposomalized adenosine.

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