LT2010104A - Rekombinantinis viengrandžio antikūno fragmentas, neutralizuojantis vaginolizino citolitinį aktyvumą - Google Patents

Rekombinantinis viengrandžio antikūno fragmentas, neutralizuojantis vaginolizino citolitinį aktyvumą

Info

Publication number
LT2010104A
LT2010104A LT2010104A LT2010104A LT2010104A LT 2010104 A LT2010104 A LT 2010104A LT 2010104 A LT2010104 A LT 2010104A LT 2010104 A LT2010104 A LT 2010104A LT 2010104 A LT2010104 A LT 2010104A
Authority
LT
Lithuania
Prior art keywords
antibody fragment
recombinant antibody
single stranded
cytolytic activity
vaginolysin
Prior art date
Application number
LT2010104A
Other languages
English (en)
Other versions
LT5855B (lt
Inventor
Milda PLEČKAITYTĖ
Edita MIŠTINIENĖ
Jonas Henrikas Pesliakas
Gintautas ŽVIRBLIS
Aurelija ŽVIRBLIENĖ
Original Assignee
Uab Profarma, ,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uab Profarma, , filed Critical Uab Profarma, ,
Priority to LT2010104A priority Critical patent/LT5855B/lt
Priority to EP11718794A priority patent/EP2563810A1/en
Priority to PCT/LT2011/000004 priority patent/WO2012078014A1/en
Publication of LT2010104A publication Critical patent/LT2010104A/lt
Publication of LT5855B publication Critical patent/LT5855B/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Genų inžinerijos būdu iš hibridomos 9B4 (DSM ACC2991) gautas rekombinantinis antikūno fragmentas scFv. Jis buvo sėkmingai išgrynintas ir parodytas jo specifiškumas VLY citolizinui iš G.vaginalis bakterijų ir efektyvi citolitinio aktyvumo neutralizacija. Išradimo pavyzdžiai parodo, kad rekombinantinis antikūno fragmentas scFv gali būti naudojamas terapiniais ir profilaktiniais tikslais gydant patologijas, sukeltas G.vaginalis infekcijos, arba išvengiant jų. Gauti viengrandžiai antikūno fragmentai (scFv) atskleidė svarbius privalumus prieš tradiciniu keliu gautus monokloninius antikūnus: pagaminti scFv galima paprastesniu ir pigesniu bakterinės biosintezės būdu, dėl mažesnės molekulinės masės tokie baltymai lengviau prasiskverbia į infekcijos pažeistus audinius.
LT2010104A 2010-12-07 2010-12-07 Rekombinantinis viengrandžio antikūno fragmentas, neutralizuojantis vaginolizino citolitinį aktyvumą LT5855B (lt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
LT2010104A LT5855B (lt) 2010-12-07 2010-12-07 Rekombinantinis viengrandžio antikūno fragmentas, neutralizuojantis vaginolizino citolitinį aktyvumą
EP11718794A EP2563810A1 (en) 2010-12-07 2011-03-28 Recombinant single-chain antibody fragment neutralizing the cytolytic activity of vaginolysin
PCT/LT2011/000004 WO2012078014A1 (en) 2010-12-07 2011-03-28 Recombinant single-chain antibody fragment neutralizing the cytolytic activity of vaginolysin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2010104A LT5855B (lt) 2010-12-07 2010-12-07 Rekombinantinis viengrandžio antikūno fragmentas, neutralizuojantis vaginolizino citolitinį aktyvumą

Publications (2)

Publication Number Publication Date
LT2010104A true LT2010104A (lt) 2012-06-25
LT5855B LT5855B (lt) 2012-08-27

Family

ID=44227537

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2010104A LT5855B (lt) 2010-12-07 2010-12-07 Rekombinantinis viengrandžio antikūno fragmentas, neutralizuojantis vaginolizino citolitinį aktyvumą

Country Status (3)

Country Link
EP (1) EP2563810A1 (lt)
LT (1) LT5855B (lt)
WO (1) WO2012078014A1 (lt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9803017B2 (en) 2013-07-05 2017-10-31 University Of Washington Through Its Center For Commercialization Soluble MIC neutralizing monoclonal antibody for treating cancer
CN104173985A (zh) * 2014-09-02 2014-12-03 郭书堂 一种治疗妇女产后受凉的药及其制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8802112B2 (en) * 2008-03-15 2014-08-12 The Trustees Of Columbia University In The City Of New York Treatment and prevention of Gardnerella vaginalis infections
LT5705B (en) 2009-02-23 2011-01-25 Uab Profarma Monoclonal antibodies against vaginolysin

Also Published As

Publication number Publication date
EP2563810A1 (en) 2013-03-06
WO2012078014A1 (en) 2012-06-14
LT5855B (lt) 2012-08-27

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Effective date: 20231207