LT3359671T - Dnr vektoriai, transpozonai ir transpozazės, skirti eukariotinio genomo modifikacijai - Google Patents

Dnr vektoriai, transpozonai ir transpozazės, skirti eukariotinio genomo modifikacijai

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Publication number
LT3359671T
LT3359671T LTEPPCT/US2016/055824T LT16055824T LT3359671T LT 3359671 T LT3359671 T LT 3359671T LT 16055824 T LT16055824 T LT 16055824T LT 3359671 T LT3359671 T LT 3359671T
Authority
LT
Lithuania
Prior art keywords
eukariotic
transposases
transposons
genome
modification
Prior art date
Application number
LTEPPCT/US2016/055824T
Other languages
English (en)
Inventor
Jeremy Minshull
Mark Welch
Sridhar Govindrajan
Maggie Lee
Kate CAVES
Jon Ness
Original Assignee
Dna Twopointo Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Twopointo Inc. filed Critical Dna Twopointo Inc.
Publication of LT3359671T publication Critical patent/LT3359671T/lt

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N2830/00—Vector systems having a special element relevant for transcription
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    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

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  • Health & Medical Sciences (AREA)
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  • Zoology (AREA)
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  • Wood Science & Technology (AREA)
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  • Virology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
LTEPPCT/US2016/055824T 2015-10-08 2016-10-06 Dnr vektoriai, transpozonai ir transpozazės, skirti eukariotinio genomo modifikacijai LT3359671T (lt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562239109P 2015-10-08 2015-10-08
US201662325872P 2016-04-21 2016-04-21
US201662373422P 2016-08-11 2016-08-11
PCT/US2016/055824 WO2017062668A2 (en) 2015-10-08 2016-10-06 Dna vectors, transposons and transposases for eukaryotic genome modification

Publications (1)

Publication Number Publication Date
LT3359671T true LT3359671T (lt) 2021-12-10

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LTEPPCT/US2016/055824T LT3359671T (lt) 2015-10-08 2016-10-06 Dnr vektoriai, transpozonai ir transpozazės, skirti eukariotinio genomo modifikacijai

Country Status (8)

Country Link
US (6) US10435696B2 (lt)
EP (2) EP3359671B1 (lt)
CA (1) CA3001312A1 (lt)
DK (1) DK3359671T3 (lt)
ES (1) ES2891087T3 (lt)
HU (1) HUE056009T2 (lt)
LT (1) LT3359671T (lt)
WO (1) WO2017062668A2 (lt)

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IL287088B2 (en) * 2019-04-08 2023-09-01 Dna Twopointo Inc Transposition of nucleic acid constructs into eukaryotic genomes with amylase transposases
US11060098B2 (en) 2019-04-08 2021-07-13 Dna Twopointo Inc. Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias
WO2020210236A1 (en) * 2019-04-08 2020-10-15 Dna Twopointo Inc. Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis
KR102937659B1 (ko) * 2020-02-19 2026-03-10 우시 바이올로직스 아일랜드 리미티드 강화된 발현 시스템 및 그 사용방법
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JP7419168B2 (ja) * 2020-06-10 2024-01-22 株式会社東芝 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法
JP2024533038A (ja) * 2021-09-08 2024-09-12 メタゲノミ，インク． カーゴヌクレオチド配列を転位するための系及び方法
US20250002876A1 (en) * 2021-11-04 2025-01-02 Saliogen Therapeutics, Inc. Mobile elements and chimeric constructs thereof
WO2023240152A1 (en) 2022-06-07 2023-12-14 Upside Foods, Inc. Engineering cell lines capable of proliferation in growth factor free media formulations
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