LT3375889T - Vienos ląstelės analizė - Google Patents

Vienos ląstelės analizė

Info

Publication number
LT3375889T
LT3375889T LTEP17305297.8T LT17305297T LT3375889T LT 3375889 T LT3375889 T LT 3375889T LT 17305297 T LT17305297 T LT 17305297T LT 3375889 T LT3375889 T LT 3375889T
Authority
LT
Lithuania
Prior art keywords
single cell
cell analysis
analysis
cell
Prior art date
Application number
LTEP17305297.8T
Other languages
English (en)
Inventor
Marcel Reichen
Bingqing Shen
Arnaud REITZ
Sami ELLOUZE
Annabelle GERARD
Original Assignee
Hifibio Sas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hifibio Sas filed Critical Hifibio Sas
Publication of LT3375889T publication Critical patent/LT3375889T/lt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0803Disc shape
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/629Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
LTEP17305297.8T 2017-03-17 2017-03-17 Vienos ląstelės analizė LT3375889T (lt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17305297.8A EP3375889B1 (en) 2017-03-17 2017-03-17 Single cell analysis

Publications (1)

Publication Number Publication Date
LT3375889T true LT3375889T (lt) 2020-06-25

Family

ID=58448501

Family Applications (2)

Application Number Title Priority Date Filing Date
LTEP17305297.8T LT3375889T (lt) 2017-03-17 2017-03-17 Vienos ląstelės analizė
LTEPPCT/EP2018/056551T LT3619319T (lt) 2017-03-17 2018-03-15 Pavienės ląstelės analizė

Family Applications After (1)

Application Number Title Priority Date Filing Date
LTEPPCT/EP2018/056551T LT3619319T (lt) 2017-03-17 2018-03-15 Pavienės ląstelės analizė

Country Status (9)

Country Link
US (2) US11427853B2 (lt)
EP (2) EP3375889B1 (lt)
JP (1) JP7050803B2 (lt)
CN (3) CN110431239B (lt)
AU (2) AU2018234084B2 (lt)
IL (2) IL317396A (lt)
LT (2) LT3375889T (lt)
SG (1) SG11201907569SA (lt)
WO (1) WO2018167218A1 (lt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3906318A1 (en) * 2019-01-06 2021-11-10 10X Genomics, Inc. Methods and systems for enrichment of barcodes
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
US20220364147A1 (en) * 2019-11-15 2022-11-17 Miltenyi Biotec B.V. & Co. KG Color and bardcoded beads for single cell indexing
JP7107535B2 (ja) * 2020-01-10 2022-07-27 シンクサイト株式会社 新規細胞表現型スクリーニング方法
WO2021255727A1 (en) * 2020-06-16 2021-12-23 Onecell Medical Ltd. Systems and methods for personalized adoptive cell therapy
CN116134301A (zh) * 2020-07-22 2023-05-16 赛多利斯司特蒂姆塞尔卡有限公司 液滴微阵列
US20230348891A1 (en) * 2020-08-21 2023-11-02 Hifibio (Shanghai) Limited Functional screening using droplet-based microfluidics
KR102482205B1 (ko) * 2020-11-18 2022-12-28 주식회사 뉴로소나 압전 복합체 제조 방법
EP4267301A1 (en) 2020-12-22 2023-11-01 HiFiBiO SAS Microfluidic methods and systems
CN118647871A (zh) * 2022-01-18 2024-09-13 深圳华大生命科学研究院 确定多种物质与细胞作用关系的方法和微井阵列芯片

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
AU2004229440B2 (en) 2003-04-10 2010-08-12 President And Fellows Of Harvard College Formation and control of fluidic species
WO2005021151A1 (en) 2003-08-27 2005-03-10 President And Fellows Of Harvard College Electronic control of fluidic species
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2010128157A1 (en) 2009-05-07 2010-11-11 Universite De Strasbourg Microfluidic system and methods for highly selective droplet fusion
US9695390B2 (en) 2010-08-23 2017-07-04 President And Fellows Of Harvard College Acoustic waves in microfluidics
EP3305918B1 (en) * 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
WO2013177206A2 (en) * 2012-05-21 2013-11-28 Fluidigm Corporation Single-particle analysis of particle populations
DK3089822T3 (da) * 2013-12-30 2022-05-02 Atreca Inc Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder
AU2015250034B2 (en) 2014-04-21 2019-06-20 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN107873054B (zh) * 2014-09-09 2022-07-12 博德研究所 用于复合单细胞核酸分析的基于微滴的方法和设备
FR3027396B1 (fr) 2014-10-15 2016-11-25 Espci Innov Procede d'analyse du contenu de gouttes et appareil associe
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
EP3303633B1 (en) * 2015-05-26 2024-02-21 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, and systems
EP3314013B1 (en) * 2015-06-26 2025-09-10 European Molecular Biology Laboratory Cell barcoding in microfluidics
US11421270B2 (en) * 2015-08-25 2022-08-23 The Broad Institute, Inc. Screening of nucleic acid sequence variations via random loading of optically-encoded particles

Also Published As

Publication number Publication date
CN119506397A (zh) 2025-02-25
CN114045328B (zh) 2024-11-15
IL269168B2 (en) 2025-05-01
CN114045328A (zh) 2022-02-15
EP3375889A1 (en) 2018-09-19
EP3619319A1 (en) 2020-03-11
US11427853B2 (en) 2022-08-30
US20230063190A1 (en) 2023-03-02
CN110431239A (zh) 2019-11-08
SG11201907569SA (en) 2019-09-27
IL269168B1 (en) 2025-01-01
IL269168A (en) 2019-11-28
JP7050803B2 (ja) 2022-04-08
LT3619319T (lt) 2021-10-11
AU2018234084A1 (en) 2019-09-26
WO2018167218A1 (en) 2018-09-20
AU2018234084B2 (en) 2024-06-13
IL317396A (en) 2025-02-01
CA3055005A1 (en) 2018-09-20
AU2024219390A1 (en) 2024-09-26
CN110431239B (zh) 2021-07-09
JP2020513807A (ja) 2020-05-21
EP3375889B1 (en) 2019-12-11
US20200199649A1 (en) 2020-06-25
EP3619319B1 (en) 2021-06-23

Similar Documents

Publication Publication Date Title
IL271435A (en) T cell receptors
EP3428031C0 (en) DRIVER ASSISTANCE DESIGN ANALYSIS SYSTEM
IL262128A (en) T cell receptors
IL262124A (en) T cell receptors
IL262146A (en) T cell receptors
EP3858365C0 (en) T CAR CELLS OPTIMIZED WITH GOLD
HUE059159T2 (hu) T-sejt-receptorok
DK3448882T3 (da) Anti-kk-lc-1-t-cellereceptorer
DK4206640T3 (da) Mikrotom
EP3372985C0 (en) ANALYSIS DEVICE
CL2020000951A1 (es) Célula.
DK3303568T3 (da) Celle
UA38793S (uk) Акумуляторна батарея
EP3573464A4 (en) B CELL ENGINEERING
LT3375889T (lt) Vienos ląstelės analizė
DE112018006483A5 (de) Elektrochemisches System
EP3286210C0 (en) T CELL RECEPTOR
DK3766582T3 (da) Cellekultur
EP3613123A4 (en) PERFORMANCE-BASED BATTERY CONFIGURATIONS
HUE072082T2 (hu) Akkumulátor
LT3433270T (lt) T ląstelių receptoriai
EP3551764A4 (en) ELECTROPHEROGRAM ANALYSIS
EP3448986A4 (en) CELL CULTURE
DK3513192T3 (da) Celledødsbiomarkør
IL270880A (en) Cell culture methods