LT3375889T - Vienos ląstelės analizė - Google Patents

Vienos ląstelės analizė

Info

Publication number
LT3375889T
LT3375889T LTEP17305297.8T LT17305297T LT3375889T LT 3375889 T LT3375889 T LT 3375889T LT 17305297 T LT17305297 T LT 17305297T LT 3375889 T LT3375889 T LT 3375889T
Authority
LT
Lithuania
Prior art keywords
single cell
cell analysis
analysis
cell
Prior art date
Application number
LTEP17305297.8T
Other languages
English (en)
Inventor
Marcel Reichen
Bingqing Shen
Arnaud REITZ
Sami ELLOUZE
Annabelle GERARD
Original Assignee
Hifibio Sas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hifibio Sas filed Critical Hifibio Sas
Publication of LT3375889T publication Critical patent/LT3375889T/lt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0803Disc shape
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/629Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
LTEP17305297.8T 2017-03-17 2017-03-17 Vienos ląstelės analizė LT3375889T (lt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17305297.8A EP3375889B1 (en) 2017-03-17 2017-03-17 Single cell analysis

Publications (1)

Publication Number Publication Date
LT3375889T true LT3375889T (lt) 2020-06-25

Family

ID=58448501

Family Applications (2)

Application Number Title Priority Date Filing Date
LTEP17305297.8T LT3375889T (lt) 2017-03-17 2017-03-17 Vienos ląstelės analizė
LTEPPCT/EP2018/056551T LT3619319T (lt) 2017-03-17 2018-03-15 Pavienės ląstelės analizė

Family Applications After (1)

Application Number Title Priority Date Filing Date
LTEPPCT/EP2018/056551T LT3619319T (lt) 2017-03-17 2018-03-15 Pavienės ląstelės analizė

Country Status (9)

Country Link
US (2) US11427853B2 (lt)
EP (2) EP3375889B1 (lt)
JP (1) JP7050803B2 (lt)
CN (3) CN114045328B (lt)
AU (2) AU2018234084B2 (lt)
IL (2) IL269168B2 (lt)
LT (2) LT3375889T (lt)
SG (1) SG11201907569SA (lt)
WO (1) WO2018167218A1 (lt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202107418SA (en) * 2019-01-06 2021-08-30 10X Genomics Inc Methods and systems for enrichment of barcodes
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
JP7749555B2 (ja) * 2019-11-15 2025-10-06 ミルテニー バイオテック ベー.フェー. ウント コー. カー・ゲー シングルセルインデクシングのための色およびバーコード付きのビーズ
EP4089181A4 (en) * 2020-01-10 2024-05-22 Thinkcyte, Inc. NOVEL CELLULAR PHENOTYPE SCREENING METHODS
WO2021255727A1 (en) * 2020-06-16 2021-12-23 Onecell Medical Ltd. Systems and methods for personalized adoptive cell therapy
CN116134301A (zh) * 2020-07-22 2023-05-16 赛多利斯司特蒂姆塞尔卡有限公司 液滴微阵列
KR102482205B1 (ko) 2020-11-18 2022-12-28 주식회사 뉴로소나 압전 복합체 제조 방법
CA3202814A1 (en) 2020-12-22 2022-06-30 Hifibio Sas Microfluidic methods and systems
CN118647871A (zh) * 2022-01-18 2024-09-13 深圳华大生命科学研究院 确定多种物质与细胞作用关系的方法和微井阵列芯片

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP2127736A1 (en) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Formation and control of fluidic species
BRPI0414004A (pt) 2003-08-27 2006-10-24 Harvard College controle eletrÈnico de espécies fluìdicas
EP2248578B1 (en) 2005-03-04 2012-06-06 President and Fellows of Harvard College Method for forming multiple emulsions
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2010128157A1 (en) 2009-05-07 2010-11-11 Universite De Strasbourg Microfluidic system and methods for highly selective droplet fusion
EP2608878A4 (en) 2010-08-23 2017-11-15 President and Fellows of Harvard College Acoustic waves in microfluidics
EP2823064B1 (en) * 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
CN106460033B (zh) * 2013-12-30 2021-12-24 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
LT3134536T (lt) 2014-04-21 2020-05-11 President And Fellows Of Harvard College Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui
EP4105337A1 (en) * 2014-09-09 2022-12-21 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
FR3027396B1 (fr) 2014-10-15 2016-11-25 Espci Innov Procede d'analyse du contenu de gouttes et appareil associe
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
EP3303633B1 (en) * 2015-05-26 2024-02-21 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, and systems
WO2016207441A1 (en) * 2015-06-26 2016-12-29 European Molecular Biology Laboratory Cell barcoding in microfluidics
EP3736337B1 (en) * 2015-08-25 2023-11-01 The Broad Institute, Inc. Method of optically encoding discrete volumes using optically-encoded particles

Also Published As

Publication number Publication date
US20200199649A1 (en) 2020-06-25
JP2020513807A (ja) 2020-05-21
IL269168B1 (en) 2025-01-01
CA3055005A1 (en) 2018-09-20
CN110431239B (zh) 2021-07-09
CN114045328B (zh) 2024-11-15
US11427853B2 (en) 2022-08-30
AU2018234084B2 (en) 2024-06-13
EP3375889A1 (en) 2018-09-19
CN119506397A (zh) 2025-02-25
IL317396A (en) 2025-02-01
US20230063190A1 (en) 2023-03-02
LT3619319T (lt) 2021-10-11
AU2024219390A1 (en) 2024-09-26
AU2018234084A1 (en) 2019-09-26
JP7050803B2 (ja) 2022-04-08
WO2018167218A1 (en) 2018-09-20
SG11201907569SA (en) 2019-09-27
CN110431239A (zh) 2019-11-08
EP3619319A1 (en) 2020-03-11
CN114045328A (zh) 2022-02-15
EP3619319B1 (en) 2021-06-23
IL269168A (en) 2019-11-28
EP3375889B1 (en) 2019-12-11
IL269168B2 (en) 2025-05-01

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