LT3645717T - Moduliniai nukleorūgščių adapteriai - Google Patents

Moduliniai nukleorūgščių adapteriai

Info

Publication number
LT3645717T
LT3645717T LTEPPCT/EP2018/067246T LT18067246T LT3645717T LT 3645717 T LT3645717 T LT 3645717T LT 18067246 T LT18067246 T LT 18067246T LT 3645717 T LT3645717 T LT 3645717T
Authority
LT
Lithuania
Prior art keywords
nucleic acid
acid adapters
modular nucleic
modular
adapters
Prior art date
Application number
LTEPPCT/EP2018/067246T
Other languages
English (en)
Inventor
Daniel KLASS
Alexander LOVEJOY
Seyed Hamid MIREBRAHIM
Amrita PATI
Original Assignee
F. Hoffmann-La Roche Ag
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F. Hoffmann-La Roche Ag, Roche Diagnostics Gmbh filed Critical F. Hoffmann-La Roche Ag
Publication of LT3645717T publication Critical patent/LT3645717T/lt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LTEPPCT/EP2018/067246T 2017-06-27 2018-06-27 Moduliniai nukleorūgščių adapteriai LT3645717T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762525595P 2017-06-27 2017-06-27
PCT/EP2018/067246 WO2019002366A1 (en) 2017-06-27 2018-06-27 MODULAR ADAPTERS OF NUCLEIC ACID

Publications (1)

Publication Number Publication Date
LT3645717T true LT3645717T (lt) 2021-12-10

Family

ID=62751007

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEPPCT/EP2018/067246T LT3645717T (lt) 2017-06-27 2018-06-27 Moduliniai nukleorūgščių adapteriai

Country Status (8)

Country Link
US (2) US20200109397A1 (lt)
EP (1) EP3645717B1 (lt)
JP (1) JP7030857B2 (lt)
CN (1) CN110785493B (lt)
DK (1) DK3645717T3 (lt)
ES (1) ES2898644T3 (lt)
LT (1) LT3645717T (lt)
WO (1) WO2019002366A1 (lt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3795685A1 (en) * 2019-09-20 2021-03-24 Sophia Genetics S.A. Methods for dna library generation to facilitate the detection and reporting of low frequency variants
US12037640B2 (en) * 2021-01-08 2024-07-16 Agilent Technologies, Inc. Sequencing an insert and an identifier without denaturation
KR20230163434A (ko) * 2021-03-29 2023-11-30 일루미나, 인코포레이티드 라이브러리에서 dna 손상을 평가하고 앰플리콘 크기 바이어스를 정규화하기 위한 조성물 및 방법
CA3242053A1 (en) 2021-12-07 2023-06-15 Caribou Biosciences, Inc. A method of capturing crispr endonuclease cleavage products
WO2024073412A2 (en) * 2022-09-26 2024-04-04 The Rockefeller University Compositions and methods for synthesizing multi-indexed sequencing libraries
WO2025078657A1 (en) 2023-10-13 2025-04-17 F. Hoffmann-La Roche Ag Amplification-free target enrichment workflow for direct detection of nucleic acid modifications
WO2025117738A1 (en) * 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
WO2025231432A1 (en) 2024-05-03 2025-11-06 Caribou Biosciences, Inc. In vivo gene editing with crispr systems

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP3252174B1 (en) * 2012-10-15 2020-07-01 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
EP3191628B1 (en) * 2014-09-12 2022-05-25 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acids
CN106435744B (zh) * 2015-08-13 2022-02-01 生捷科技控股公司 使用y-接头和消失限制位点的文库构建
EP3408406B1 (en) * 2016-01-29 2022-06-15 F. Hoffmann-La Roche AG A novel y-shaped adaptor for nucleic acid sequencing and method of use

Also Published As

Publication number Publication date
US20200109397A1 (en) 2020-04-09
EP3645717A1 (en) 2020-05-06
EP3645717B1 (en) 2021-08-11
CN110785493B (zh) 2023-12-05
US20230081899A1 (en) 2023-03-16
WO2019002366A1 (en) 2019-01-03
CN110785493A (zh) 2020-02-11
ES2898644T3 (es) 2022-03-08
DK3645717T3 (da) 2021-11-08
JP2020529833A (ja) 2020-10-15
JP7030857B2 (ja) 2022-03-07

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