LU508355B1 - A method for producing and applying freeze-dried matsutake mushroom powder for skin whitening, photoaging, anti-inflammatory, anti-glycation, and anti-aging - Google Patents

Abstract

The invention relates to the technical field of active ingredient production and specifically relates to a process for producing and using freeze-dried matsutake powder for skin lightening, photoaging, anti-inflammation, anti-glycation, and anti-aging. The process comprises the following steps: collection and pretreatment, fermentation treatment, separation and extraction, and refinement treatment. The advantage of this invention is that the proposed method for producing and using freeze-dried matsutake powder for skin lightening, photoaging, anti-inflammation, anti-glycation, and anti-aging achieves efficient extraction of matsutake polysaccharides and other active ingredients from matsutake by optimizing the fermentation conditions and separation and extraction techniques, thereby increasing the yield and purity of the matsutake polysaccharides and other active ingredients.

Description

A process for the production and use of freeze-dried matsutake- LU508355

Mushroom powder for skin lightening, photoaging inhibition, anti-inflammatory, anti-

Glycation and anti-aging

Technical area

The invention relates to the technical field of the production of active ingredients and specifically relates to a process for the production and use of freeze-dried

Matsutake mushroom powder for skin lightening, photoaging, anti-inflammatory, anti-

Glycation and anti-aging.

Technology in the background

Matsutake (also known as matsutake mushroom or pine mushroom) is a valuable edible mushroom with high nutritional value and rich in various bioactive ingredients. Among these is

Matsutake polysaccharide is a unique B-glucan that has great potential for application in medicine, cosmetics and due to its strong antioxidant, anti-inflammatory, antiglycationic and photoaging properties.

dietary supplements.

Currently, the production of ß-glucans is mainly based on chemical

Synthesis methods, however, are often associated with high costs and significant environmental impact.

Therefore, there is a need for a process for the production of freeze-dried

Matsutake powder, which has skin lightening, photoaging, anti-inflammatory, antiglycationic and anti-aging effects, to solve the existing problems of high cost and

environmental pollution in the production of matsutake polysaccharides. This process can improve the yield and purity of matsutake polysaccharides and at the same time

Reduce production costs and environmental impact.

Content of the invention

The aim of the invention is to provide a process for the preparation and application of freeze-dried matsutake powder for skin whitening, photoaging inhibition, anti-inflammatory, anti-glycation and anti-aging in order to achieve the above

Background technology to solve the problems of high costs and environmental pollution in the conventional production of matsutake polysaccharides. The process can

Improve the yield and purity of Matsutake polysaccharides while increasing the

Reduce production costs and environmental impact.

To achieve this goal, the invention provides the following technical solution: A

Process for producing freeze-dried matsutake powder for skin lightening,

Anti-aging, anti-inflammatory, anti-glycation and anti-aging, which includes the following steps:

Collection and pretreatment;

fermentation treatment;

Separation and extraction;

Finishing treatment.

Preferably, the specific procedure for collection and pretreatment includes:

Collect fresh matsutake and select samples with bright color and full tissue. Each kilogram of matsutake contains approximately 100 grams of ergothioneine;

Clean to remove surface contaminants and cut into small pieces of 0.5cm x 0.5cm for further processing.

Preferably, the specific procedure for the fermentation treatment includes: LU508355

Put the sliced matsutake into a fermentation container, add a suitable amount

Add water and a special fermentation additive so that the ratio of

Fermentation liquid to Matsutake is 10:1;

Fermentation takes place at a constant temperature of 30 ° C for usually 24

hours, with the conversion rate of ergothioneine reaching 80% after 24 hours of fermentation.

Preferably, the specific procedure for separation and extraction includes:

After completion of the fermentation, the fermentation liquid is filtered from the

Matsutake residues are separated, yielding about 10 liters of fermentation liquid;

The fermentation liquid is evaporated to one-fifth of its original volume to remove excess water;

High performance liquid chromatography analysis shows that the Matsutake

Polysaccharide content in the concentrated liquid is up to 100 mg per milliliter.

Preferably, the specific procedure for the finishing treatment includes:

The synthesized Matsutake polysaccharide is obtained by crystallization, filtration and

Drying purified to remove unreacted matsutake polysaccharide and other

to remove impurities;

The purity of the refined ergothioneine can be over 20%.

An application of the process for the production of freeze-dried

Matsutake powder for skin lightening, photoaging, anti-inflammatory, anti-

Glycation and anti-aging derived matsutake polysaccharide is found in the area

Skin lightening and tightening takes place.

An application of the process for the production of freeze-dried

Matsutake powder for skin lightening, photoaging, anti-inflammatory, anti-

Glycation and anti-aging derived matsutake polysaccharide is used in the field of

Photoaging inhibition takes place.

An application of the process for the production of freeze-dried

Matsutake powder for skin lightening, photoaging, anti-inflammatory, anti-

Glycation and anti-aging derived matsutake polysaccharide is used in the field of anti-

Glycation occurs.

An application of the process for the production of freeze-dried

Matsutake powder for skin lightening, photoaging, anti-inflammatory, anti-

Glycation and anti-aging derived matsutake polysaccharide is used in the field of anti-

Aging treatment takes place.

Compared to the prior art, the invention offers the following advantages:

The application of the process for the production of freeze-dried

Matsutake powder for skin lightening, photoaging, anti-inflammatory, anti-

Glycation and anti-aging of Matsutake polysaccharide as proposed in this invention is made possible by optimizing the fermentation conditions and the

Separation and extraction techniques enable efficient extraction of matsutake polysaccharides and other active components from matsutake. This increases the yield and purity of matsutake polysaccharides, with ergothioneine yields reaching up to 80% and ergothioneine content exceeding 20%. By using the biological

Fermentation process instead of the conventional chemical synthesis method is 44508355

The risk of environmental pollution that could occur during chemical synthesis is avoided. Furthermore, the raw materials used in this process, namely matsutake, are a renewable resource, contributing to environmental friendliness and sustainable development.

The produced Matsutake polysaccharide has been used in the fields of medicine, cosmetics and

Dietary supplements have a wide range of applications. In the medical field, it can be used to treat inflammatory diseases, intestinal diseases and other chronic

diseases; in cosmetics it can be used as a key ingredient in anti-

anti-aging and sun protection products; and in the field of dietary supplements, it can be used as an additive in functional foods and beverages to

To strengthen the immune system, prevent diseases and slow down aging.

The invention also solves the existing problems of conventional Matsutake polysaccharide

production, such as high costs and environmental pollution, and enables increased yield and purity of Matsutake polysaccharide while reducing production costs and

Environmental pollution.

Description of the attached drawings

Figure 1 shows the flow chart of the process of this invention.

Detailed description

In order to clearly and completely describe the object, the technical concept and the advantages of this invention, the embodiments of the invention are explained in more detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described here represent only a part of the invention and do not include all embodiments. They serve only to illustrate the embodiments of the invention and not to limit the same. All other embodiments that can be devised by persons with ordinary skill in the art without inventive step

activity fall within the scope of this invention.

Example 1

Please see Figure 1. The invention provides a technical solution: A

Process for producing freeze-dried matsutake powder for skin lightening,

Anti-aging, anti-inflammatory, anti-glycation, and anti-aging. The procedure includes the following steps: 1. Collection and pretreatment: 1.1 Collection in detail:

Timing: Matsutake should be harvested during its prime growth period to ensure that the collected mushrooms are fresh and rich in active ingredients.

Sample selection: When collecting mushrooms, choose mushrooms with bright colors and full tissue. These samples generally represent higher nutritional value and a richer content of bioactive components. Each kilogram of matsutake contains approximately 200

Grams of B-glucan, an important precursor for the production of Matsutake-

polysaccharides.

Collection method: Matsutake should be harvested either manually or mechanically, carefully to avoid damage. After harvesting, a

Pretreatment should be carried out to preserve freshness and activity. 1.2 Pretreatment includes in detail:

Cleaning: The collected matsutake mushrooms are placed in clean water and gently stirred to remove soil, sand, and other impurities from the surface. 4508355. During the cleaning process, running water should be used, and excessive force should be avoided to avoid damaging the structure of the matsutake.

Draining: The cleaned matsutake mushrooms should be placed on a clean cloth or sieve to allow them to drain naturally. During the draining process, direct

Sunlight and high temperatures should be avoided to avoid impairing the quality of the Matsutake.

Cutting: The drained Matsutake mushrooms are cut into small pieces of 0.5 cm x 0.5 cm. This size facilitates the subsequent fermentation and

Extraction process and contributes to the efficiency of polysaccharide extraction. Sharp tools should be used during cutting and the cleanliness of the work environment should be ensured.

Pretreatment: After cutting, the Matsutake pieces can be pretreated, for example by soaking in a special solution or by briefly

Treatment at low temperatures to activate the bioactive ingredients or remove potential contaminants. The specific procedure in this step can be adapted as needed. 2. Fermentation Treatment: 2.1 Preparation Phase includes in detail:

Cleaning the fermentation vessel: First, the fermentation vessel must be thoroughly cleaned and disinfected to remove any microbiological contaminants that could affect the fermentation process.

Preparation of the Matsutake: The already cleaned, drained and cut

Matsutake pieces are prepared for use in the fermentation vessel.

Preparation of the fermentation agent: Depending on the amount of Matsutake and the desired fermentation effect, an appropriate amount of the specially prepared

This fermentation agent can contain one or more

Contain microorganisms such as bacteria, yeasts or molds, which promote the conversion of

Matsutake polysaccharides can promote. 2.2 Fermentation process includes in detail:

Mixing and adding: The matsutake pieces are placed in the fermentation vessel, then an appropriate amount of water is added so that the ratio of fermentation liquid to matsutake is approximately 10:1. The special fermentation agent is then evenly sprinkled into the fermentation vessel to ensure complete mixing with the matsutake.

Temperature regulation: The temperature of the fermentation vessel is kept at a constant

Temperature of 30 ° C, which is the optimal temperature for the fermentation of the

Matsutake. At this temperature, the microorganisms in the fermentation medium can actively grow and carry out metabolic processes, thereby facilitating the conversion of the Matsutake

Polysaccharides are promoted.

Stirring and aeration: During the fermentation process, the fermentation liquid is stirred regularly to ensure sufficient contact between Matsutake and

Fermentation liquid and to prevent the settling of substances in the fermentation liquid. Depending on the requirements of the fermentation medium, it may be necessary to

Fermentation tanks should be supplied with an appropriate amount of oxygen or aerated to ensure

To meet the respiratory needs of microorganisms. LU508355

Monitoring and control: During the fermentation process, the pH, the

Temperature, oxygen content, and other parameters of the fermentation liquid should be carefully monitored and adjusted as needed. Furthermore, the conversion rate of the matsutake polysaccharides should be checked regularly to ensure that the fermentation process is proceeding as expected. 2.3 Completion of fermentation and subsequent processing includes in detail:

Fermentation time: After about 24 hours of fermentation, the conversion rate of the

Matsutake polysaccharides typically contain 80%. Once the desired conversion rate is reached, the fermentation process can be completed.

Sampling and testing: After completion of fermentation, a small amount of sample is taken from the fermentation vessel to determine the conversion rate of the Matsutake

Polysaccharides and the quality of the fermentation liquid.

Subsequent processing: The fermentation liquid is filtered or

Centrifugation to separate the fermentation liquid from the matsutake residues. The fermentation liquid can then be used in the subsequent separation and

extraction steps, while the matsutake residues can be processed for other purposes or used as fertilizer. 3. Separation and Extraction: 3.1 The specific steps of filtration and separation include:

After the completion of the fermentation process of the Matsutake, the fermentation liquid is first separated from the Matsutake polysaccharides by filtration.

Filtration process can be carried out using various suitable filtration devices such as filter presses,

Vacuum filters, etc., are used to ensure effective separation of solids and liquids. This step yields approximately 10 liters of fermentation liquid, which forms the basis for the subsequent extraction of matsutake polysaccharides. 3.2 The specific steps for concentrating the fermentation liquid include:

After receiving the fermentation liquid, the concentration treatment takes place. The aim of this

The purpose of this step is to remove excess water from the fermentation liquid to make the subsequent extraction of matsutake polysaccharides more effective. Concentration can be achieved by various methods such as vacuum distillation, vacuum evaporation,

reverse osmosis, etc. During the actual implementation, the degree and speed of concentration must be controlled to avoid negative influence on the

Matsutake polysaccharides. After concentration, the volume of

Fermentation liquid can be reduced to about one-fifth of its original volume, which increases the efficiency of the subsequent extraction process. 3.3 The specific steps of high-performance liquid chromatography (HPLC)-

Analysis includes:

After preparing the concentrated liquid, high performance liquid chromatography (HPLC) analysis is required to determine the content of matsutake polysaccharides in the concentrated liquid. HPLC is a highly sensitive and high resolution analytical method that allows for accurate separation and detection of the individual

components in complex samples. HPLC analysis can determine the exact content of matsutake polysaccharides in the concentrated liquid, which is important for the

Optimization of the extraction process and quality control is of great importance.

3.4 Extraction process: LU508355

After the content of matsutake polysaccharides in the concentrated liquid has been determined, the actual extraction process can be carried out. The specific

Extraction method is selected depending on the actual circumstances, e.g.

Solvent extraction, solid phase extraction, supercritical fluid extraction, etc. 3.5 The specific steps of cleaning and drying include:

The matsutake polysaccharide solution obtained by extraction can still contain some

Contains impurities and therefore needs to be further cleaned.

Purification methods include crystallization, recrystallization, chromatographic separation, etc. The purified matsutake polysaccharide can then be processed into a solid product by drying. Drying methods include vacuum drying, spray drying, etc. The dried matsutake polysaccharide should be stored in a dry, cool, and ventilated

Store in a cool, dry place to avoid moisture and high temperatures. 4. Finishing treatment: 4.1 Crystallization:

Selection of the solvent: First, according to the solubility of the Matsutake

A suitable solvent is selected depending on the type of polysaccharide and the type of impurities.

Solvent should be chosen so that the matsutake polysaccharides can be completely dissolved while the impurities are dissolved as little as possible.

Dissolution: The synthesized crude matsutake polysaccharide is added to the solvent and completely dissolved by heating and stirring.

Cooling and crystallization: Once the solution temperature has cooled to a certain range, the matsutake polysaccharides begin to crystallize. By controlling the

By adjusting the cooling rate and stirring speed, uniformly sized and highly crystallized matsutake polysaccharide crystals can be obtained. 4.2 Filtration:

Selection of filter material: Select a suitable filter material for filtration of the

Matsutake polysaccharide crystals, e.g., filter paper or filter cloth. The filter material should have good

have filter properties and chemical stability.

Filtration process: The solution containing the Matsutake polysaccharide crystals is filtered into the

filter, and the vacuum pump is switched on for vacuum filtration. During the

During filtration, care must be taken to ensure that the filter material remains smooth and the

Filtration speed is constant to avoid breaking of the crystals or clogging of the

filter material to be avoided.

Washing: The Matsutake polysaccharide crystals obtained by filtration are washed with a small amount of solvent to remove adhering impurities on the

Crystal surface to be removed. 4.3 Drying:

Selection of drying method: Depending on the properties and quantity of Matsutake

Polysaccharides, a suitable drying method is chosen, e.g., vacuum drying or

Oven drying.

Adjustment of drying conditions: Adjust the drying temperature, time and other parameters so that the matsutake polysaccharides are not thermally decomposed or oxidized during the drying process.

Drying process: The filtered and washed matsutake polysaccharide crystals

; are placed in the dryer, and the drying device is started. While 64508355

During the drying process, the crystals must be turned regularly to ensure even

To ensure drying. 4.4 Purity test:

Selection of analytical method: Use high-performance liquid chromatography (HPLC), mass spectrometry (MS), or other analytical methods to verify the purity of the purified matsutake polysaccharides.

Analysis of the results: The purity of Matsutake polysaccharides is determined by the

If the purity does not meet the requirements, the

Finishing must be carried out again or the finishing conditions must be adjusted.

Example 2

Based on Embodiment 1, an application of the freeze-dried matsutake powder obtained by the process for producing skin whitening,

Photoaging inhibition, anti-inflammatory, anti-glycation and anti-aging

Matsutake polysaccharide, which is used in the field of skin lightening and tightening.

Matsutake polysaccharide has a strong inhibitory effect on melanin synthesis and tyrosinase activity in cells and can be used by intervening in the

Melanin deposition process can achieve a lightening effect. In addition, the inhibiting

The effect of Matsutake polysaccharide on tyrosinase activity is stronger than that of some known lightening agents such as arbutin and can increase the

Effectively inhibit tyrosinase activity, highlighting its natural and safe properties and showing good potential for application and industrial development.

Example 3

Based on Embodiment 1, an application of the freeze-dried matsutake powder obtained by the process for producing skin whitening,

Photoaging inhibition, anti-inflammatory, anti-glycation and anti-aging

Matsutake polysaccharide, which is used in the field of photoaging inhibition, is presented.

Ultraviolet radiation is one of the main causes of light-induced skin aging.

Matsutake polysaccharide can resist damage from UV rays, the formation of free

Reduce radicals in the skin and protect skin cells from oxidative stress. Therefore,

Matsutake polysaccharide has a wide range of application potential in the cosmetic field and can be used as an effective anti-aging and anti-photoaging ingredient.

Example 4

Based on Embodiment 1, an application of the freeze-dried matsutake powder obtained by the process for producing skin whitening,

Photoaging inhibition, anti-inflammatory, anti-glycation and anti-aging

Matsutake polysaccharide, which is used in the field of anti-inflammatory and anti-

Glycation is used.

Inflammation and glycation reactions are also important factors contributing to aging. Matsutake polysaccharide can inhibit the production and activity of inflammatory factors, thus reducing cell damage caused by inflammatory reactions. At the same time, it can inhibit glycation reactions and prevent the accumulation of

glycation products in the body. These effects contribute to the

To delay the aging process of cells and maintain the health and youthfulness of the body A/508355.

Example 5

Based on Embodiment 1, an application of the freeze-dried matsutake powder obtained by the process for producing skin whitening,

Photoaging inhibition, anti-inflammatory, anti-glycation and anti-aging

Matsutake polysaccharide, which is used in anti-aging treatment, is presented.

The Matsutake mushroom also has a significant effect in combating skin aging.

The numerous nutrients it contains can work synergistically and improve the overall

Increase the body's performance, thereby slowing down the signs of aging.

As we age, the human body gradually ages, but Matsutake extract can effectively help slow down the aging process.

Although the embodiments of this invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications,

Adaptations and variations of these embodiments can be made without affecting

Principles and spirit of the invention. The scope of this invention is defined by the appended claims and their equivalents.

Claims (9)

Claims LU508355 1. A process for producing and using freeze-dried matsutake powder for skin whitening, photoaging, anti-inflammation, anti-glycation, and anti-aging, characterized in that the process comprises the following steps: collection and pretreatment; fermentation treatment; separation and extraction; and refining treatment. 2. The method for producing freeze-dried matsutake powder for skin whitening, photoaging, anti-inflammation, anti-glycation, and anti-aging according to claim 1, characterized in that the specific steps of collection and pretreatment include: collecting fresh matsutake and selecting samples with bright color and full tissue; each kilogram of matsutake contains about 100 grams of matsutake polysaccharides; cleaning to remove surface contaminants and cutting into small pieces of 0.5 cm x 0.5 cm for further processing. 3. The method for producing freeze-dried matsutake powder for skin whitening, photoaging, anti-inflammation, anti-glycation, and anti-aging according to claim 1, characterized in that the specific steps of the fermentation treatment include: placing the cut matsutake pieces into the fermentation vessel, adding an appropriate amount of water and a specific fermentation agent so that the ratio of the fermentation liquid to matsutake is 10:1; fermentation is carried out at a constant temperature of 30°C, usually for a period of 24 hours, and after 24 hours, the conversion rate of matsutake can reach up to 80%. 4. The process for producing freeze-dried matsutake powder for skin whitening, photoaging, anti-inflammation, anti-glycation, and anti-aging according to claim 1, characterized in that the specific steps of separation and extraction include: After completion of fermentation, the fermentation liquid is separated from the matsutake residue by filtration, obtaining about 10 liters of fermentation liquid; The fermentation liquid is evaporated to one-fifth of its original volume to remove excess water; High-performance liquid chromatography analysis shows that the content of matsutake polysaccharides in the concentrated liquid is up to 100 milligrams per milliliter. 5. The method for producing freeze-dried matsutake powder for skin whitening, photoaging inhibition, anti-inflammation, anti-glycation and anti-aging according to claim 1, characterized in that the specific steps of the refining treatment include: The synthesized matsutake polysaccharide is purified by crystallization, filtration and drying to remove unreacted matsutake polysaccharide and other To remove impurities; LUS08355 The purity of refined matsutake polysaccharide can be over 20%. 6. Fine application of the matsutake polysaccharide obtained by the process for producing freeze-dried matsutake powder for skin lightening, photoaging inhibition, anti-inflammation, anti-glycation and anti-aging according to any one of claims 1-5, characterized in that it is used in the field of skin lightening and tightening. 7. An application of the matsutake polysaccharide obtained by the process for producing freeze-dried matsutake powder for skin lightening, photoaging inhibition, anti-inflammation, anti-glycation and antiaging according to any one of claims 1-5, characterized in that it is used in the field of photoaging inhibition. 8. Fine application of the matsutake polysaccharide obtained by the process for producing freeze-dried matsutake powder for skin lightening, photoaging inhibition, anti-inflammation, anti-glycation and anti-aging according to any one of claims 1-5, characterized in that it is used in the field of anti-glycation. 9. Fine application of the matsutake polysaccharide obtained by the process for producing freeze-dried matsutake powder for skin lightening, photoaging inhibition, anti-inflammation, anti-glycation and anti-aging according to any one of claims 1-5, characterized in that it is used in the field of anti-aging treatment.