MA27631A1 - Procede de culture cellulaire n'utilisant pas de composants d'origine animale - Google Patents

Procede de culture cellulaire n'utilisant pas de composants d'origine animale

Info

Publication number
MA27631A1
MA27631A1 MA28488A MA28488A MA27631A1 MA 27631 A1 MA27631 A1 MA 27631A1 MA 28488 A MA28488 A MA 28488A MA 28488 A MA28488 A MA 28488A MA 27631 A1 MA27631 A1 MA 27631A1
Authority
MA
Morocco
Prior art keywords
cell culture
cells
animal
relates
animal origin
Prior art date
Application number
MA28488A
Other languages
English (en)
Inventor
Ghislaine Louise Aert Brigitte
Jules Maurice Ghislain Yves
Jane Gonze Marie-Monique
Solange Lucie Knott Isabelle
Maggetto Carine
Original Assignee
Glaxosmithkline Biolog Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxosmithkline Biolog Sa filed Critical Glaxosmithkline Biolog Sa
Publication of MA27631A1 publication Critical patent/MA27631A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A61P31/22—Antivirals for DNA viruses for herpes viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32451—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCÉDÉ DE CULTURE CELLULAIRE N'UTILISANT PAS DE COMPOSANTS D'ORIGINE ANIMALE La présente invention concerne un procédé de culture de cellules animales, de préférence des cellules diploïdes humaines ancrage-dépendantes, en absence de composants exogènes d'origine animale primaire, et un milieu de culture cellulaire essentiellement exempt de composants exogènes d'origine animale primaire approprié pour réaliser ledit procédé. En particulier, l'invention concerne un milieu de culture cellulaire qui comprend au moins un, et de manières davantage préférées plusieurs facteurs de croissance exogènes n'ayant pas une origine animale. La présente invention concerne également un procédé destiné à la culture de cellules animales, de préférence des cellules diploïdes humaines ancrage-dépendantes, dans un milieu selon l'invention, impliquant l'utilisation d'un substitut de trypsine d'origine non-animale pour le repiquage des cellules. L'invention concerne en outre un procédé de production de virus, de vaccins viraux et équivalent. (Figure 1)
MA28488A 2003-03-03 2005-09-09 Procede de culture cellulaire n'utilisant pas de composants d'origine animale MA27631A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0304799.0A GB0304799D0 (en) 2003-03-03 2003-03-03 Novel method

Publications (1)

Publication Number Publication Date
MA27631A1 true MA27631A1 (fr) 2005-11-01

Family

ID=9953995

Family Applications (1)

Application Number Title Priority Date Filing Date
MA28488A MA27631A1 (fr) 2003-03-03 2005-09-09 Procede de culture cellulaire n'utilisant pas de composants d'origine animale

Country Status (17)

Country Link
US (1) US8034617B2 (fr)
EP (3) EP2192175A1 (fr)
JP (2) JP4787739B2 (fr)
KR (1) KR101242090B1 (fr)
CN (2) CN1756837B (fr)
AU (1) AU2004217807B2 (fr)
BR (1) BRPI0407636A (fr)
CA (1) CA2517327A1 (fr)
CO (1) CO5601047A2 (fr)
GB (1) GB0304799D0 (fr)
IS (1) IS7992A (fr)
MA (1) MA27631A1 (fr)
MX (1) MXPA05009096A (fr)
NO (1) NO20054124L (fr)
NZ (1) NZ541908A (fr)
RU (1) RU2369634C2 (fr)
WO (1) WO2004078955A1 (fr)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2404682T3 (es) 2003-12-11 2013-05-28 Isto Technologies Inc. Sistema de cartílago particulado
EP1739167B1 (fr) * 2004-04-19 2015-08-12 DENKA SEIKEN Co., Ltd. Méthode de production de virus
CA2585547A1 (fr) * 2004-10-29 2006-05-11 Centocor, Inc. Compositions de milieux chimiquement definis
CA2601006C (fr) * 2005-03-10 2012-10-23 Kyoritsu Seiyaku Corporation Lignee cellulaire cultivable sans composant derive d'un animal, procede destine a l'etablir, procede de production de virus utilisant la lignee cellulaire et procede de productionde vaccins
EP1891207A2 (fr) * 2005-06-03 2008-02-27 Biovitrum AB Procede de culture de cellules animales comprenant l'apport de peptones derives de plantes
AU2006282754A1 (en) 2005-08-26 2007-03-01 Zimmer, Inc. Implants and methods for repair, replacement and treatment of joint disease
EP1764117A1 (fr) 2005-09-20 2007-03-21 Zimmer GmbH Implant pour la réparation de défauts cartilagineux et son procédé de préparation
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
AU2008240191B2 (en) 2007-04-12 2013-09-19 Zimmer, Inc. Compositions and methods for tissue repair
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
US8637312B2 (en) 2008-01-09 2014-01-28 Cellca Gmbh Mammalian culture media with polyamine and iron
SG2014014385A (en) 2009-02-17 2014-04-28 Glaxosmithkline Biolog Sa Inactivated dengue virus vaccine with aluminium-free adjuvant
ES2533074T3 (es) * 2010-04-26 2015-04-07 Novartis Ag Medio de cultivo celular mejorado
CN101831415B (zh) * 2010-05-12 2012-05-30 江苏博立生物制品有限公司 一种提高糖化酶发酵水平的方法
RU2612915C2 (ru) * 2011-02-28 2017-03-13 Президент Энд Феллоуз Ов Харвард Колледж Система культивирования клеток
ES2673471T3 (es) 2011-12-08 2018-06-22 Yeda Research And Development Co. Ltd. Células pulmonares fetales de mamífero y uso terapéutico de las mismas
US9493744B2 (en) 2012-06-20 2016-11-15 Genentech, Inc. Methods for viral inactivation and other adventitious agents
US20130281355A1 (en) * 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
JP6041199B2 (ja) * 2012-09-14 2016-12-07 国立大学法人大阪大学 三次元細胞培養体の製造方法
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
EP3310388B1 (fr) 2015-06-18 2021-10-06 Yeda Research and Development Co., Ltd. Protocoles de conditionnement et utilisation de ceux-ci pour la régénération de tissu
US10004795B2 (en) * 2015-09-08 2018-06-26 Fundacao Butantan Process for preparing an attenuated tetravalent dengue vaccine
WO2017109698A1 (fr) 2015-12-22 2017-06-29 Glaxosmithkline Biologicals Sa Formulation immunogène
CN105567628B (zh) * 2016-01-30 2019-07-12 令世鑫 一种全悬浮培养mdck细胞的低血清培养基
CN105695393B (zh) * 2016-03-24 2019-09-10 友康恒业生物科技（北京）有限公司 一种mdck细胞无血清培养基及其用途和制备
CN105779376B (zh) * 2016-03-24 2019-08-30 友康恒业生物科技（北京）有限公司 一种流感病毒分离无血清培养基及其用途和制备
KR101925079B1 (ko) * 2017-05-24 2018-12-04 가천대학교 산학협력단 Dna 정제-증폭을 위한 통합형 마이크로 디바이스 및 이를 이용한 dna 정제-증폭 방법
CN107432931B (zh) * 2017-08-03 2018-04-13 长春祈健生物制品有限公司 一种微载体培养水痘病毒制备疫苗的方法
BR112021014116A2 (pt) * 2019-01-17 2021-09-21 Boehringer Ingelheim Animal Health USA Inc. Meio sem soro para produção de vacinas aviárias e seus usos
EP4084629A4 (fr) 2019-12-31 2023-12-27 Air Protein, Inc. Compositions alimentaires à haute teneur en protéines
MX2022009132A (es) * 2020-01-24 2022-08-22 Air Protein Inc Hidrolizados de proteina derivados de microorganismos, y metodos de preparacion y uso de los mismos.
CN113373107B (zh) * 2021-01-29 2022-12-30 上海生物制品研究所有限责任公司 一种大规模制备传代鸡胚细胞生产病毒的方法
WO2024039216A1 (fr) * 2022-08-19 2024-02-22 셀미트주식회사 Procédé de culture de cellules animales ou d'une lignée de cellules animales
CN115627255B (zh) * 2022-12-01 2023-04-07 天信和(苏州)生物科技有限公司 一种采用低血清培养基培养人二倍体细胞的方法
CN119979445B (zh) * 2025-04-10 2025-09-09 四川大学 一种培养基、口腔黏膜上皮细胞模型，及其制备方法和用途

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL94611A (en) * 1989-06-05 1994-12-29 Organogenesis Inc Medium for cell cultures containing insulin or growth factor similar to insulin, transferrin or iron ion, triiodothyronine or thyroxine and method of use
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
DE4115722A1 (de) 1991-05-14 1992-11-19 Boehringer Mannheim Gmbh Serumfreies medium zur kultivierung von saeugerzellen
RU2057176C1 (ru) * 1992-05-20 1996-03-27 Институт генетики АН Республики Молдова Питательная среда для культивирования клеток человека и животных
DZ1706A1 (fr) * 1992-08-07 2002-02-17 Merck & Co Inc Vaccin contre le virus de l'hepatite a.
CA2144317C (fr) 1992-09-18 2004-08-03 Ann W. Funkhouser Vaccins contre l'hepatite a
CN1088984A (zh) * 1993-11-11 1994-07-06 江西省医学科学研究所 一种用于造血祖细胞培养的无血清培养基
HRP950097A2 (en) 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
EP0791055B1 (fr) 1994-11-10 2012-01-25 Baxter Healthcare S.A. Procede de production d'agents biologiques dans un milieu de culture sans proteines
WO1998000521A1 (fr) * 1996-06-28 1998-01-08 The Green Cross Corporation Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives
WO1998004680A1 (fr) * 1996-07-26 1998-02-05 University Of Manitoba Milieu ne contenant pas de serum, destine a la culture de cellules mammaliennes dependantes d'un support
EP1983044B1 (fr) * 1996-10-10 2016-08-10 Life Technologies Corporation Support de culture cellulaire animale comportant des agents nutritifs à base de plantes
AU7984498A (en) * 1997-06-25 1999-01-04 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Serum-free cell growth medium
US6656719B1 (en) * 1997-10-27 2003-12-02 Merck & Co., Inc. Serum-free, low-protein media for rotavirus vaccine production
FR2775983B1 (fr) 1998-03-13 2000-11-10 Pasteur Merieux Serums Vacc Milieu et procede de propagation et de multiplication virales
WO1999057246A1 (fr) 1998-05-01 1999-11-11 Life Technologies, Inc. Milieux de culture de cellules animales comprenant des elements nutritifs derives des vegetaux
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
US6623959B2 (en) * 2001-06-13 2003-09-23 Ethicon, Inc. Devices and methods for cell harvesting

Also Published As

Publication number Publication date
NO20054124D0 (no) 2005-09-05
EP2192175A1 (fr) 2010-06-02
AU2004217807B2 (en) 2009-06-25
MXPA05009096A (es) 2005-10-19
JP2010273683A (ja) 2010-12-09
IS7992A (is) 2005-08-18
CN1756837B (zh) 2010-05-26
RU2369634C2 (ru) 2009-10-10
KR20050105509A (ko) 2005-11-04
KR101242090B1 (ko) 2013-03-08
JP4787739B2 (ja) 2011-10-05
CN1756837A (zh) 2006-04-05
US20060183224A1 (en) 2006-08-17
NO20054124L (no) 2005-09-27
US8034617B2 (en) 2011-10-11
EP2186885A1 (fr) 2010-05-19
EP1599580A1 (fr) 2005-11-30
NZ541908A (en) 2008-04-30
CA2517327A1 (fr) 2004-09-16
CO5601047A2 (es) 2006-01-31
RU2005125606A (ru) 2006-03-10
WO2004078955A1 (fr) 2004-09-16
AU2004217807A1 (en) 2004-09-16
BRPI0407636A (pt) 2006-02-21
CN101200705A (zh) 2008-06-18
JP2006519023A (ja) 2006-08-24
GB0304799D0 (en) 2003-04-09

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