MA32307B1 - عملية لتحديد معدل الهيموجلوبين و ترقيم وتمييز خلايا الدم البيضاء في محيط مناسب - Google Patents
عملية لتحديد معدل الهيموجلوبين و ترقيم وتمييز خلايا الدم البيضاء في محيط مناسبInfo
- Publication number
- MA32307B1 MA32307B1 MA33352A MA33352A MA32307B1 MA 32307 B1 MA32307 B1 MA 32307B1 MA 33352 A MA33352 A MA 33352A MA 33352 A MA33352 A MA 33352A MA 32307 B1 MA32307 B1 MA 32307B1
- Authority
- MA
- Morocco
- Prior art keywords
- hemoglobin
- blood cells
- white blood
- numbering
- determining
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 102000001554 Hemoglobins Human genes 0.000 title abstract 3
- 108010054147 Hemoglobins Proteins 0.000 title abstract 3
- 210000000265 leukocyte Anatomy 0.000 title abstract 3
- 210000004369 blood Anatomy 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 2
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- RBNPZEHAODHBPZ-UHFFFAOYSA-M dihydroxyaluminium Chemical compound O.O.NCC(=O)O[Al] RBNPZEHAODHBPZ-UHFFFAOYSA-M 0.000 abstract 1
- 125000001453 quaternary ammonium group Chemical group 0.000 abstract 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 abstract 1
- 238000002798 spectrophotometry method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
يتعلق الاختراع بطريقة لتحديد، في عينة من الدم، نسبة الهيموجلوبين، ترقيم وتمايز خلايا الدم البيضاء، التي تتألف من الخطوات التالية: لدينا عينة من الدم في وسط متساوي التوتر أو مفرط الحموضة، لا تتجاوز درجة حموضته 10، في غياب أيونات السيانيد وتضم على الأقل ملح الأمونيوم الرباعية وعامل محافظ ، يتم الكشف عن المركب المشكَّل بين الهيموغلوبين ومجموعات الأمونيوم الرباعي من خلال القياس الطيفي لشدة الضوء ،ثم يتم عدّ كريات الدم البيضاء ويتم تمييزها على الأقل إلى ساكنات فرعية ثلاثة، يتم انجاز الطريقة المذكورة في غياب أي رابط آخر.يتعلق الاختراع أيضا بوسط لتنفيذ الطريقة.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0802729A FR2931557A1 (fr) | 2008-05-20 | 2008-05-20 | Procede de determination du taux d'hemoglobine, de numeration et de differenciation des globules blancs et milieu adapte |
| PCT/FR2009/000593 WO2009150327A2 (fr) | 2008-05-20 | 2009-05-20 | Procede de determination du taux d'hemoglobine, de numeration et de differenciation des globules blancs et milieu adapte |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MA32307B1 true MA32307B1 (ar) | 2011-05-02 |
Family
ID=40227784
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MA33352A MA32307B1 (ar) | 2008-05-20 | 2010-11-12 | عملية لتحديد معدل الهيموجلوبين و ترقيم وتمييز خلايا الدم البيضاء في محيط مناسب |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2291662A2 (ar) |
| JP (1) | JP2011521251A (ar) |
| FR (1) | FR2931557A1 (ar) |
| MA (1) | MA32307B1 (ar) |
| RU (1) | RU2010146751A (ar) |
| WO (1) | WO2009150327A2 (ar) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8614066B2 (en) * | 2009-08-26 | 2013-12-24 | Abbott Laboratories | Method of using ligand-free lysing agent in hemoglobin analysis |
| CN116609243A (zh) | 2018-04-28 | 2023-08-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液分析方法、血液分析系统及存储介质 |
| JP2025164429A (ja) * | 2024-04-19 | 2025-10-30 | 公益財団法人とかち財団 | 血乳検査方法及び血乳検査装置 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4286963A (en) * | 1979-11-23 | 1981-09-01 | Coulter Electronics, Inc. | Differential lymphoid-myeloid determination of leukocytes in whole blood |
| US4853338A (en) * | 1987-05-20 | 1989-08-01 | Technicon Instruments Corporation | Cyanide-free hemoglobin reagent |
| JP2836865B2 (ja) * | 1989-10-23 | 1998-12-14 | 東亜医用電子株式会社 | 血液中の白血球およびヘモグロビンの測定用試薬 |
| WO1996002841A1 (en) * | 1994-07-14 | 1996-02-01 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
| US6214625B1 (en) * | 1999-04-28 | 2001-04-10 | Coulter International Corp. | Composition and method for differentiation of basophils and eosinophils in blood |
| US7235404B2 (en) * | 2005-05-04 | 2007-06-26 | Beckman Coulter, Inc. | Cyanide-free lytic reagent composition and method of use for hemoglobin and white blood cell measurement |
-
2008
- 2008-05-20 FR FR0802729A patent/FR2931557A1/fr not_active Withdrawn
-
2009
- 2009-05-20 JP JP2011510019A patent/JP2011521251A/ja active Pending
- 2009-05-20 RU RU2010146751/15A patent/RU2010146751A/ru unknown
- 2009-05-20 WO PCT/FR2009/000593 patent/WO2009150327A2/fr not_active Ceased
- 2009-05-20 EP EP09761878A patent/EP2291662A2/fr not_active Withdrawn
-
2010
- 2010-11-12 MA MA33352A patent/MA32307B1/ar unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009150327A3 (fr) | 2010-03-25 |
| RU2010146751A (ru) | 2012-06-27 |
| EP2291662A2 (fr) | 2011-03-09 |
| FR2931557A1 (fr) | 2009-11-27 |
| JP2011521251A (ja) | 2011-07-21 |
| WO2009150327A2 (fr) | 2009-12-17 |
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