MA43987A - Détermination de la pureté de protéine multimère - Google Patents

Détermination de la pureté de protéine multimère

Info

Publication number
MA43987A
MA43987A MA043987A MA43987A MA43987A MA 43987 A MA43987 A MA 43987A MA 043987 A MA043987 A MA 043987A MA 43987 A MA43987 A MA 43987A MA 43987 A MA43987 A MA 43987A
Authority
MA
Morocco
Prior art keywords
purity
determination
multimer protein
multimer
protein
Prior art date
Application number
MA043987A
Other languages
English (en)
Inventor
Jiann-Kae Luo
Kathir Muthusamy
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MA43987A publication Critical patent/MA43987A/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Optics & Photonics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
MA043987A 2015-05-12 2016-05-11 Détermination de la pureté de protéine multimère MA43987A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562160341P 2015-05-12 2015-05-12

Publications (1)

Publication Number Publication Date
MA43987A true MA43987A (fr) 2018-12-19

Family

ID=56072452

Family Applications (2)

Application Number Title Priority Date Filing Date
MA043987A MA43987A (fr) 2015-05-12 2016-05-11 Détermination de la pureté de protéine multimère
MA056416A MA56416A (fr) 2015-05-12 2016-05-11 Détermination de la pureté de protéines multimériques

Family Applications After (1)

Application Number Title Priority Date Filing Date
MA056416A MA56416A (fr) 2015-05-12 2016-05-11 Détermination de la pureté de protéines multimériques

Country Status (10)

Country Link
US (3) US10520511B2 (fr)
EP (2) EP3885368A1 (fr)
JP (3) JP7240812B2 (fr)
AR (1) AR104610A1 (fr)
AU (2) AU2016261854B2 (fr)
CA (1) CA2984010A1 (fr)
ES (1) ES2881359T3 (fr)
MA (2) MA43987A (fr)
PL (1) PL3294775T3 (fr)
WO (1) WO2016183222A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202016008838U1 (de) 2015-11-30 2020-01-20 Intabio, Inc. Vorrichtungen zur Probencharakterisierung
EP3541365A1 (fr) 2016-11-21 2019-09-25 Just Biotherapeutics, Inc. Formulations d'aflibercecept et leurs utilisations
KR102578087B1 (ko) 2017-08-17 2023-09-18 저스트-에보텍 바이오로직스, 아이엔씨. 숙주세포 갈렉틴(galectins) 및 다른 오염물(contaminant)로부터 글리코실화 단백질을 정제하는 방법
WO2019099965A1 (fr) 2017-11-20 2019-05-23 Just Biotherapeutics, Inc. Formulations d'aflibercept contenant un sel de lysine en tant qu'agent de tonicification et leurs utilisations
CA3089842A1 (fr) 2018-01-29 2019-08-01 Intabio, Inc. Dispositifs, procedes et kits de caracterisation d'echantillon
WO2019232397A1 (fr) 2018-05-31 2019-12-05 Intabio, Inc. Logiciel pour systèmes microfluidiques faisant interface avec la spectrométrie de masse
US11573226B2 (en) 2018-09-10 2023-02-07 Genentech, Inc. Systems and methods for affinity capillary electrophoresis
TWI850255B (zh) * 2018-09-10 2024-08-01 美商建南德克公司 用於親和毛細管電泳之系統及方法
US11782023B2 (en) * 2018-12-19 2023-10-10 Regeneron Pharmaceuticals, Inc. Ce-western applications for antibody development
KR20210122803A (ko) * 2019-01-31 2021-10-12 리제너론 파아마슈티컬스, 인크. 항체 전하 이질성의 고유 마이크로유체 ce-ms 분석
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies
US20240201131A1 (en) * 2021-04-14 2024-06-20 Dh Technologies Development Pte. Ltd. Native Fluorescence Detection for Protein Analysis in Capillary Electrophoresis
EP4437344A1 (fr) * 2021-11-25 2024-10-02 F. Hoffmann-La Roche AG Quantification de faibles quantités de produits secondaires d'anticorps

Family Cites Families (22)

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Publication number Priority date Publication date Assignee Title
AUPP385598A0 (en) 1998-06-02 1998-06-25 Life Therapeutics Limited Purification of antibodies
US6472179B2 (en) * 1998-09-25 2002-10-29 Regeneron Pharmaceuticals, Inc. Receptor based antagonists and methods of making and using
WO2000063683A1 (fr) * 1999-04-20 2000-10-26 Target Discovery, Inc. Procedes de cartographie peptidique de polypeptides, etablissement de profils et systeme de base de donnees bioinformatique
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
AUPQ743300A0 (en) * 2000-05-11 2000-06-01 Life Therapeutics Limited Separation of avian antibodies
JP3727317B2 (ja) 2002-03-08 2005-12-14 エイエスエムエル ネザランドズ ベスローテン フエンノートシャップ リソグラフィに使用するためのマスク、マスクを作成する方法、リソグラフィ装置、およびデバイス製造方法
US20090134028A1 (en) * 2005-09-22 2009-05-28 Chemicals Evaluation And Research Institute Method of Estimating Effect of Test Chemical on Living Organisms
EP1803814A1 (fr) * 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Procédé d'amélioration la capacité de sélection d'anticorps dans une bibliothèque d'affichage de phages
WO2007114325A1 (fr) * 2006-03-31 2007-10-11 Chugai Seiyaku Kabushiki Kaisha Procédé de modification d'anticorps pour purifier un anticorps bispécifique
ES2522615T3 (es) 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas
MX336725B (es) * 2007-09-26 2016-01-28 Chugai Pharmaceutical Co Ltd Metodo de modificacion del punto isoelectrico de anticuerpos medinate la sustitucion de aminoacidos en region de determinacion de complementariedad (cdr).
JP4977588B2 (ja) 2007-12-07 2012-07-18 富士フイルム株式会社 イムノクロマトグラフ方法
KR101747103B1 (ko) 2009-06-26 2017-06-14 리제너론 파마슈티칼스 인코포레이티드 천연 면역글로불린 포맷을 가지는 용이하게 분리된 이중특이성 항체
WO2012162068A2 (fr) 2011-05-21 2012-11-29 Macrogenics, Inc. Domaines de liaison du sérum déimmunisé et leur utilisation pour prolonger la demi-vie du sérum
GB2504139B (en) * 2012-07-20 2014-12-31 Argen X Bv Antibodies to highly conserved targets produced by the immunisation of Camelidae species
EP2604624A1 (fr) * 2011-12-13 2013-06-19 University College Dublin National University Of Ireland, Dublin Peptides de liaison de l'inhibiteur de protéase de Bowman-Birk (BBO) et utilisations associées pour la séparation, la détection ou la purification du BBI.
EP2825559B1 (fr) 2012-03-13 2019-02-27 Novimmune SA Anticorps bispécifiques aisément isolés avec un format d'immunoglobuline native
CN104582476B (zh) 2012-06-05 2017-03-08 瑞泽恩制药公司 使用共同轻链制备完全人双特异性抗体的方法
TWI675044B (zh) * 2012-11-14 2019-10-21 美商再生元醫藥公司 重組細胞表面捕捉蛋白質
WO2015024896A1 (fr) * 2013-08-19 2015-02-26 F. Hoffmann-La Roche Ag Séparation d'anticorps bispécifiques et sous-produits de la production d'anticorps bispécifiques utilisant la chromatographie sur hydroxyapatite
CA2922979A1 (fr) * 2013-09-03 2015-03-12 Novimmune S.A. Molecules de liaison bispecifiques facilement isolees au format natif, mais possedant des regions constantes mutees

Also Published As

Publication number Publication date
WO2016183222A4 (fr) 2017-01-05
JP2018528389A (ja) 2018-09-27
US20240280587A1 (en) 2024-08-22
EP3885368A1 (fr) 2021-09-29
JP7137663B2 (ja) 2022-09-14
US20200166522A1 (en) 2020-05-28
MA56416A (fr) 2022-05-04
JP7240812B2 (ja) 2023-03-16
US12158473B2 (en) 2024-12-03
EP3294775A1 (fr) 2018-03-21
AU2016261854B2 (en) 2022-05-26
JP2021152544A (ja) 2021-09-30
WO2016183222A1 (fr) 2016-11-17
CA2984010A1 (fr) 2016-11-17
US20170045527A1 (en) 2017-02-16
HK1252480A1 (zh) 2019-05-24
ES2881359T3 (es) 2021-11-29
US10520511B2 (en) 2019-12-31
PL3294775T3 (pl) 2021-12-13
AU2016261854A1 (en) 2017-11-09
AU2022203033A1 (en) 2022-05-26
AU2022203033B2 (en) 2025-06-26
AR104610A1 (es) 2017-08-02
EP3294775B1 (fr) 2021-07-14
JP2022171730A (ja) 2022-11-11

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