MA45721A - Procédés de purification de protéines contenant des fragments fc - Google Patents

Procédés de purification de protéines contenant des fragments fc

Info

Publication number
MA45721A
MA45721A MA045721A MA45721A MA45721A MA 45721 A MA45721 A MA 45721A MA 045721 A MA045721 A MA 045721A MA 45721 A MA45721 A MA 45721A MA 45721 A MA45721 A MA 45721A
Authority
MA
Morocco
Prior art keywords
fragments
processes
protein containing
purifying protein
purifying
Prior art date
Application number
MA045721A
Other languages
English (en)
Inventor
John K Kawooya
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of MA45721A publication Critical patent/MA45721A/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Storage Device Security (AREA)
MA045721A 2016-07-22 2017-07-21 Procédés de purification de protéines contenant des fragments fc MA45721A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201662365943P 2016-07-22 2016-07-22

Publications (1)

Publication Number Publication Date
MA45721A true MA45721A (fr) 2019-05-29

Family

ID=59656168

Family Applications (1)

Application Number Title Priority Date Filing Date
MA045721A MA45721A (fr) 2016-07-22 2017-07-21 Procédés de purification de protéines contenant des fragments fc

Country Status (12)

Country Link
US (1) US11312745B2 (fr)
EP (1) EP3487867B1 (fr)
JP (2) JP7092744B2 (fr)
KR (2) KR102435801B1 (fr)
CN (2) CN115925780B (fr)
AU (2) AU2017298984B2 (fr)
CA (2) CA3031469C (fr)
IL (2) IL285146B (fr)
MA (1) MA45721A (fr)
MX (2) MX2019000903A (fr)
SG (1) SG11201900573UA (fr)
WO (1) WO2018018011A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019343053B2 (en) * 2018-09-21 2026-04-30 Teneobio, Inc. Methods for purifying heterodimeric, multispecific antibodies
AR117650A1 (es) * 2018-10-11 2021-08-18 Amgen Inc Procesamiento posterior de constructos de anticuerpos biespecíficos
BR112021011194A2 (pt) * 2019-01-16 2021-08-31 Regeneron Pharmaceuticals, Inc. Método e sistema de identificar e quantificar a fragmentação do anticorpo
KR102286892B1 (ko) 2019-07-08 2021-08-06 삼천당제약주식회사 안과용 단백질 제제의 정제방법
CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法
CN112881675A (zh) * 2019-11-29 2021-06-01 广东菲鹏生物有限公司 IgM抗体检测稀释液
CA3174665A1 (fr) 2020-04-09 2021-10-14 Cytomx Therapeutics, Inc. Compositions contenant des anticorps activables
CN116056775B (zh) * 2020-09-16 2025-12-02 沃特世科技公司 在尺寸排阻色谱流动相中使用低浓度氨基酸的改善尺寸排阻色谱法
WO2023068740A1 (fr) * 2021-10-19 2023-04-27 주식회사 알테오젠 Procédé de purification d'une protéine de fusion ayant un domaine fc d'igg
CN113980092B (zh) * 2021-12-09 2024-05-14 上海药明生物技术有限公司 一种蛋白质亲和纯化方法
CN116165290B (zh) * 2021-12-17 2025-06-27 复旦大学 一种抗体药物研发流程中工程细胞株的筛选方法
CN116271982B (zh) * 2022-11-30 2024-01-09 杭州广科安德生物科技有限公司 一种血液高丰度蛋白免疫亲和柱的再生方法

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4968607A (en) 1987-11-25 1990-11-06 Immunex Corporation Interleukin-1 receptors
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
WO1990005183A1 (fr) 1988-10-31 1990-05-17 Immunex Corporation Recepteurs d'interleukine-4
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
ES2284161T3 (es) 1990-01-12 2007-11-01 Amgen Fremont Inc. Generacion de anticuerpos xenogenicos.
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US6713610B1 (en) 1990-01-12 2004-03-30 Raju Kucherlapati Human antibodies derived from immunized xenomice
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
WO1991018982A1 (fr) 1990-06-05 1991-12-12 Immunex Corporation Recepteurs de l'interleukine-1 de type ii
US5350683A (en) 1990-06-05 1994-09-27 Immunex Corporation DNA encoding type II interleukin-1 receptors
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
AU8507191A (en) 1990-08-29 1992-03-30 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6300129B1 (en) 1990-08-29 2001-10-09 Genpharm International Transgenic non-human animals for producing heterologous antibodies
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
ES2301158T3 (es) 1992-07-24 2008-06-16 Amgen Fremont Inc. Produccion de anticuerpos xenogenicos.
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
EP0822830B1 (fr) 1995-04-27 2008-04-02 Amgen Fremont Inc. Anticorps anti-IL-8 dérivés de xenosouris immunisées
ATE549918T1 (de) 1996-12-03 2012-04-15 Amgen Fremont Inc Menschliche antikörper, die ausdrücklich menschliches tnf alpha binden
US6271349B1 (en) 1996-12-23 2001-08-07 Immunex Corporation Receptor activator of NF-κB
AU763513B2 (en) * 1998-04-16 2003-07-24 Genentech Inc. Secretion of glycosylated proteins using a tissue plasminogen activator pro-sequence
US6660843B1 (en) 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
US20030133939A1 (en) 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
SG156672A1 (en) * 2004-10-22 2009-11-26 Amgen Inc Methods for refolding of recombinant antibodies
BRPI0611600B8 (pt) * 2005-06-17 2021-05-25 Wyeth Corp método para purificação de uma proteína tendo uma região fc a partir de um líquido fonte compreendendo a proteína e uma ou mais impurezas, e kit
CA2636109A1 (fr) * 2006-01-06 2007-07-19 Amgen Inc. Procedes et systemes d'isolation de molecules cibles de solutions complexes par chromatographie sur colonne a l'aide d'eluants contenant des solvants organiques
CN105837690A (zh) 2006-06-12 2016-08-10 新兴产品开发西雅图有限公司 具有效应功能的单链多价结合蛋白
WO2008036600A2 (fr) * 2006-09-18 2008-03-27 Genentech, Inc. Procédés de production de protéines
JP5522723B2 (ja) * 2007-05-21 2014-06-18 ノマディックバイオサイエンス株式会社 新規ポリペプチド，アフィニティークロマトグラフィー用材，及びイムノグロブリンの分離及び／又は精製方法
EP3663318A1 (fr) 2008-01-07 2020-06-10 Amgen Inc. Procédé de fabrication de molécules hétérodimères fc d'anticorps utilisant les effets de conduite électrostatique
WO2010019493A1 (fr) * 2008-08-14 2010-02-18 Merck Sharp & Dohme Corp. Procédés de purification d'anticorps à l'aide d'une chromatographie d'affinité sur protéine a
KR101741859B1 (ko) * 2009-06-22 2017-06-15 암젠 인크 화학적으로 조절된 산화환원 상태를 사용한 단백질의 재폴딩
SI3660032T1 (sl) * 2009-06-25 2026-02-27 Amgen Inc. Postopki zajemanja in čiščenja beljakovin, izraženih v nesesalskem sistemu
MX2012003396A (es) 2009-09-16 2012-04-10 Genentech Inc Complejos de proteina conteniendo helice super-enrollada y/o enlazador tether y usos de los mismos.
CN102382190B (zh) * 2010-09-01 2014-04-09 山东新时代药业有限公司 分离和去除TNFR-Fc融合蛋白中寡聚体的方法
WO2012086837A1 (fr) 2010-12-24 2012-06-28 旭化成メディカル株式会社 Procédé d'immobilisation d'une protéine a thermosensible
JP6280499B2 (ja) * 2011-07-08 2018-02-14 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Ｆｃ融合タンパク質の精製方法
US10189891B2 (en) 2012-03-28 2019-01-29 Ge Healthcare Bioprocess R&D Ab Affinity chromatography matrix
EP2889617B1 (fr) 2012-08-27 2017-10-11 Asahi Kasei Medical Co., Ltd. Procédé de purification d'anticorps au moyen d'une chromatographie sensible à la température
UY35148A (es) * 2012-11-21 2014-05-30 Amgen Inc Immunoglobulinas heterodiméricas
US20140302037A1 (en) 2013-03-15 2014-10-09 Amgen Inc. BISPECIFIC-Fc MOLECULES
WO2015063339A1 (fr) * 2013-11-04 2015-05-07 Glenmark Pharmaceuticals S.A. Production de cellules t reciblant des immunoglobulines héréro-dimériques

Also Published As

Publication number Publication date
JP7411020B2 (ja) 2024-01-10
EP3487867A2 (fr) 2019-05-29
CA3218290A1 (fr) 2018-01-25
IL264375A (en) 2019-02-28
KR102435801B1 (ko) 2022-08-25
CA3031469C (fr) 2025-11-18
CN109563125B (zh) 2022-08-09
EP3487867B1 (fr) 2026-04-01
WO2018018011A2 (fr) 2018-01-25
AU2023222915B2 (en) 2026-01-22
CN109563125A (zh) 2019-04-02
IL285146A (en) 2021-08-31
CN115925780B (zh) 2025-11-04
CA3031469A1 (fr) 2018-01-25
US11312745B2 (en) 2022-04-26
AU2023222915A1 (en) 2023-09-21
AU2017298984A1 (en) 2019-02-07
WO2018018011A3 (fr) 2018-03-01
KR20190037247A (ko) 2019-04-05
IL264375B (en) 2021-09-30
JP7092744B2 (ja) 2022-06-28
AU2017298984B2 (en) 2023-08-31
CN115925780A (zh) 2023-04-07
MX2023006291A (es) 2023-06-13
JP2019528255A (ja) 2019-10-10
KR20220120716A (ko) 2022-08-30
IL285146B (en) 2022-09-01
KR102579850B1 (ko) 2023-09-18
JP2022120138A (ja) 2022-08-17
SG11201900573UA (en) 2019-02-27
US20190127418A1 (en) 2019-05-02
MX2019000903A (es) 2019-05-15

Similar Documents

Publication Publication Date Title
EP3731863A4 (fr) Procédés de purification de virus adéno-associé
EP3436473A4 (fr) Procédé de purification de protéines de fusion fc
IL285146A (en) Methods of purifying fc-containing proteins
EP2953965A4 (fr) Procédés de purification de protéines
EP3990031A4 (fr) Procédés de purification de virus adéno-associé
EP3398965A4 (fr) Procédé pour favoriser l'efficacité de purification d'un polypeptide contenant une région fc
EP2883882A4 (fr) Procédé de purification des protéines
EP3004135A4 (fr) Procédé de purification de protéine
EP3429615A4 (fr) Procédés de purification de collagène 7
EP3240804A4 (fr) Anticorps anti-myostatine, polypeptides contenant des variants de régions fc, et procédés d'utilisation
MX392274B (es) Cartuchos utiles en limpieza de soluciones de dialisis.
EP3387023A4 (fr) Procédés de récupération d'hydrocarbure
MA39774A (fr) Procédés chimiques pour produire des nucléotides étiquetés
EP2575847A4 (fr) Procédés de purification de polypeptides
FR3014901B1 (fr) Procede de purification de virus ou vecteurs viraux enveloppes
EP3472177C0 (fr) Purification d'anticorps multispécifiques
FR3025515B1 (fr) Procede de purification d'un anticorps monoclonal
IL261040B (en) Protein purification
EP2975020A4 (fr) Procédé de purification de 1,5-diaminopentane
MA41941A (fr) Procédé de purification de protéines
PL3307996T3 (pl) Układ oczyszczania gazów spalinowych
EP3412623A4 (fr) Procédé de purification
EP3438426A4 (fr) Système de purification d'échappement
EP3318552A4 (fr) Procédé de purification de méthionine
EP3302760A4 (fr) Procédés d'élimination de contaminants d'un effluent de déshydrogénation