MA52739A - Endonucléases cpf1 mutantes - Google Patents

Endonucléases cpf1 mutantes

Info

Publication number
MA52739A
MA52739A MA052739A MA52739A MA52739A MA 52739 A MA52739 A MA 52739A MA 052739 A MA052739 A MA 052739A MA 52739 A MA52739 A MA 52739A MA 52739 A MA52739 A MA 52739A
Authority
MA
Morocco
Prior art keywords
mutant cpf1
cpf1 endonucleases
endonucleases
mutant
cpf1
Prior art date
Application number
MA052739A
Other languages
English (en)
Inventor
Guillermo Montoya
Stefano Stella
Original Assignee
Univ Copenhagen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Copenhagen filed Critical Univ Copenhagen
Publication of MA52739A publication Critical patent/MA52739A/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
MA052739A 2018-06-04 2019-06-04 Endonucléases cpf1 mutantes MA52739A (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18175707 2018-06-04
EP18190950 2018-08-27

Publications (1)

Publication Number Publication Date
MA52739A true MA52739A (fr) 2021-04-21

Family

ID=66676556

Family Applications (1)

Application Number Title Priority Date Filing Date
MA052739A MA52739A (fr) 2018-06-04 2019-06-04 Endonucléases cpf1 mutantes

Country Status (12)

Country Link
US (2) US12152257B2 (fr)
EP (1) EP3802806A1 (fr)
JP (2) JP2021525541A (fr)
KR (1) KR20210028163A (fr)
CN (1) CN112424349A (fr)
AU (1) AU2019282094A1 (fr)
BR (1) BR112020024736A2 (fr)
CA (1) CA3102676A1 (fr)
IL (1) IL279153A (fr)
MA (1) MA52739A (fr)
MX (1) MX2020013229A (fr)
WO (1) WO2019233990A1 (fr)

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EP3204496A1 (fr) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions et procédés pour activer une réparation dirigée par homologie
EP3652312A1 (fr) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systèmes et procédés d'intégration ciblée et d'édition du génome et détection de celle-ci à l'aide de sites d'amorçage intégrés
AU2019291918B2 (en) 2018-06-29 2025-06-12 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
CN111521781B (zh) * 2020-02-24 2021-07-30 广州医科大学附属第一医院(广州呼吸中心) 一种用于新冠肺炎病毒SARS-CoV-2核酸的检测试剂盒及其检测方法
CN116848241A (zh) * 2020-12-17 2023-10-03 孟山都技术公司 工程化无ssDNA酶的CRISPR核酸内切酶
US20240110163A1 (en) 2021-01-29 2024-04-04 Wageningen Universiteit Crispr-associated based-editing of the complementary strand
EP4441209A4 (fr) * 2021-11-29 2025-12-17 Editas Medicine Inc Protéines effectrices crispr/cas12a modifiées et leurs utilisations
EP4486879A1 (fr) 2022-03-01 2025-01-08 Wageningen Universiteit Nickases cas12a
EP4499819A1 (fr) 2022-03-30 2025-02-05 Basf Agricultural Solutions Seed Us Llc Éditeurs de base optimisés
WO2024105633A1 (fr) 2022-11-18 2024-05-23 Kyoto Prefectural Public University Corporation Compositions pour induction de mitophagie et leurs utilisations
EP4638470A1 (fr) 2022-12-21 2025-10-29 BASF Agricultural Solutions US LLC Efficacité d'édition accrue par co-administration de rnp avec un acide nucléique
AU2024309386A1 (en) 2023-06-30 2025-12-18 Christiana Care Gene Editing Institute, Inc. Nras gene knockout for treatment of cancer
WO2025213016A2 (fr) 2024-04-04 2025-10-09 Christiana Care Gene Editing Institute, Inc. Modification génique par crispr/cas de domaines neh4 et/ou neh5 dans nrf2
WO2025226816A1 (fr) 2024-04-23 2025-10-30 Christiana Care Gene Editing Institute, Inc. Procédés d'identification et de corrélation de saut d'exon induit par crispr à des résultats phénotypiques
CN119506249B (zh) * 2024-11-29 2025-11-07 大连理工大学 一种多PAM选择的基因编辑系统NovaCas-1及其应用

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
WO2017064546A1 (fr) * 2015-09-24 2017-04-20 Crispr Therapeutics Ag Nouvelle famille d'endonucléases arn-programmables et leurs utilisations dans l'édition de génome et d'autres applications
WO2017070632A2 (fr) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Éditeurs de nucléobases et leurs utilisations
WO2017106657A1 (fr) 2015-12-18 2017-06-22 The Broad Institute Inc. Nouvelles enzymes crispr et systèmes associés
KR20260004568A (ko) 2016-04-19 2026-01-08 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
EP3445853A1 (fr) 2016-04-19 2019-02-27 The Broad Institute, Inc. Complexes cpf1 à activité d'indel réduite
CN106244591A (zh) * 2016-08-23 2016-12-21 苏州吉玛基因股份有限公司 修饰crRNA在CRISPR/Cpf1基因编辑系统中的应用
AU2017302551B2 (en) 2016-07-26 2023-04-27 The General Hospital Corporation Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
WO2018027078A1 (fr) * 2016-08-03 2018-02-08 President And Fellows Of Harard College Éditeurs de nucléobases d'adénosine et utilisations associées
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018074979A1 (fr) 2016-10-17 2018-04-26 Nanyang Technological University Protéines crispr-cas tronquées pour ciblage d'adn
IL306092A (en) 2017-03-23 2023-11-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
EP3612204A4 (fr) 2017-04-21 2021-01-27 The General Hospital Corporation Régulation de gène humain inductible, accordable et multiplex à l'aide de crispr-cpf1
WO2018195545A2 (fr) 2017-04-21 2018-10-25 The General Hospital Corporation Variantes de cpf1 (cas12a) à spécificité pam modifiée
US11834665B2 (en) 2017-05-10 2023-12-05 Regents Of The University Of Minnesota Programmable transcription factors and methods
KR102678809B1 (ko) 2017-05-18 2024-06-26 더 브로드 인스티튜트, 인코퍼레이티드 표적화된 핵산 편집을 위한 시스템, 방법 및 조성물
EP3625342B1 (fr) 2017-05-18 2022-08-24 The Broad Institute, Inc. Systèmes, procédés et compositions d'édition ciblée d'acides nucléiques
KR102655021B1 (ko) 2017-07-11 2024-04-04 시그마-알드리치 컴퍼니., 엘엘씨 표적된 게놈 변형을 개선하기 위한 뉴클레오솜 상호작용 단백질 도메인 사용
EP3658573A1 (fr) 2017-07-28 2020-06-03 President and Fellows of Harvard College Procédés et compositions pour l'évolution d'éditeurs de bases à l'aide d'une évolution continue assistée par phage (pace)
WO2019051097A1 (fr) 2017-09-08 2019-03-14 The Regents Of The University Of California Polypeptides de fusion d'endonucléase guidée par arn et procédés d'utilisation correspondants
WO2019075197A1 (fr) 2017-10-11 2019-04-18 The General Hospital Corporation Procédés de détection de désamination génomique parasite et spécifique de site induite par des technologies d'édition de base
WO2019126762A2 (fr) 2017-12-22 2019-06-27 The Broad Institute, Inc. Systèmes cas12a, procédés et compositions d'édition ciblée de bases d'arn
CN111788232A (zh) 2018-02-23 2020-10-16 上海科技大学 用于碱基编辑的融合蛋白
EP3660152A4 (fr) 2018-03-26 2020-07-15 National University Corporation Kobe University Procédé de modification d'un site cible dans un adn double brin dans une cellule
AU2019255789B2 (en) 2018-04-19 2025-11-20 The Regents Of The University Of California Compositions and methods for gene editing

Also Published As

Publication number Publication date
CA3102676A1 (fr) 2019-12-12
KR20210028163A (ko) 2021-03-11
IL279153A (en) 2021-01-31
BR112020024736A2 (pt) 2021-03-23
MX2020013229A (es) 2021-04-28
WO2019233990A1 (fr) 2019-12-12
EP3802806A1 (fr) 2021-04-14
JP2025075025A (ja) 2025-05-14
US20250163394A1 (en) 2025-05-22
JP2021525541A (ja) 2021-09-27
US20210230567A1 (en) 2021-07-29
US12152257B2 (en) 2024-11-26
AU2019282094A1 (en) 2021-01-07
CN112424349A (zh) 2021-02-26

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