MA53577B1 - Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum - Google Patents

Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum

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Publication number
MA53577B1
MA53577B1 MA53577A MA53577A MA53577B1 MA 53577 B1 MA53577 B1 MA 53577B1 MA 53577 A MA53577 A MA 53577A MA 53577 A MA53577 A MA 53577A MA 53577 B1 MA53577 B1 MA 53577B1
Authority
MA
Morocco
Prior art keywords
dna
biotech
valuable
cas9
bacterium clostridium
Prior art date
Application number
MA53577A
Other languages
English (en)
Other versions
MA53577A1 (fr
Inventor
Konstantin Viktorovich Severinov
Sergey Anatolevich Shmakov
Georgii Evgenevich Pobegalov
Aleksandra Andreevna Vasileva
Polina Anatolevna Selkova
Anatolii Nikolaevich Arseniev
Tatyana Igorevna Zyubko
Iana Vitalevna Fedorova
Daria Nikolaevna Artamonova
Ignatiy Igorevich Goryanin
Olga Sergeevna Musharova
Iuliia Valerevna Piskunova
Mikhail Alekseevich Khodorkovskiy
Tatiana Olegovna Artamonova
Marina Viktorovna Abramova
Original Assignee
Biocad Joint Stock Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocad Joint Stock Co filed Critical Biocad Joint Stock Co
Publication of MA53577A1 publication Critical patent/MA53577A1/fr
Publication of MA53577B1 publication Critical patent/MA53577B1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne une nouvelle nucléase bactérienne du système crispr-cas9 à base de la bactérie clostridium celluloliticum ainsi que son utilisation pour former des ruptures strictement spécifiques à deux brins dans la molécule d'adn. Cette nucléase possède des propriétés inhabituelles et peut s'utiliser en tant qu'instrument pour apporter des changements dans des endroits rigoureusement déterminés dans une séquence d'adn génomique d'organismes monocellullaires ou multicellullaires. De cette manière, on améliore l'universalité des systèmes crispr-cas9 disponibles, ce qui permet d'utiliser des nucléases cas9 provenant d'organismes différents pour découper un adn génomique ou plasmidique dans un plus grand nombre de lieux spécifiques et dans une plus vaste gamme de températures. L'invention permet de simplifier la rédaction du génome de la clostridium celluloliticum ayant une valeur biotechnologique.
MA53577A 2018-11-26 2019-11-26 Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum MA53577B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2018141524A RU2712497C1 (ru) 2018-11-26 2018-11-26 Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum
PCT/RU2019/050229 WO2020111983A2 (fr) 2018-11-26 2019-11-26 Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum

Publications (2)

Publication Number Publication Date
MA53577A1 MA53577A1 (fr) 2022-02-28
MA53577B1 true MA53577B1 (fr) 2022-10-31

Family

ID=69625021

Family Applications (1)

Application Number Title Priority Date Filing Date
MA53577A MA53577B1 (fr) 2018-11-26 2019-11-26 Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum

Country Status (18)

Country Link
US (1) US20220002692A1 (fr)
EP (1) EP3889269A4 (fr)
JP (1) JP7698578B2 (fr)
KR (1) KR20210118069A (fr)
CN (1) CN113785055B (fr)
AU (1) AU2019388420B2 (fr)
BR (1) BR112021010185A2 (fr)
CA (1) CA3121088A1 (fr)
CL (1) CL2021001382A1 (fr)
CO (1) CO2021006938A2 (fr)
EA (1) EA202191504A1 (fr)
MA (1) MA53577B1 (fr)
MX (1) MX2021006119A (fr)
PE (1) PE20212079A1 (fr)
PH (1) PH12021551198A1 (fr)
RU (1) RU2712497C1 (fr)
WO (1) WO2020111983A2 (fr)
ZA (1) ZA202103578B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3204496A1 (fr) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions et procédés pour activer une réparation dirigée par homologie
US12338436B2 (en) 2018-06-29 2025-06-24 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
RU2722934C1 (ru) * 2019-06-11 2020-06-05 Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий Средство разрезания днк на основе cas9 белка из бактерии pasteurella pneumotropica

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014014105B1 (pt) * 2011-12-16 2023-10-10 Targetgene Biotechnologies Ltd Método para modificar um sítio-alvo predeterminado no interior de um ácido nucleico genômico ou organelar alvo em uma célula hospedeira eucariótica por um complexo núcleoproteína
SG10201809817UA (en) * 2012-05-25 2018-12-28 Univ California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2014131833A1 (fr) * 2013-02-27 2014-09-04 Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) Édition de gène dans l'ovocyte au moyen de cas9 nucléases
ES2901396T3 (es) * 2013-03-14 2022-03-22 Caribou Biosciences Inc Composiciones y métodos de ácidos nucleicos dirigidos a ácido nucleico
EP3760719A1 (fr) * 2013-11-18 2021-01-06 CRISPR Therapeutics AG Système crips-cas, matériels et procédés
US9994831B2 (en) * 2013-12-12 2018-06-12 The Regents Of The University Of California Methods and compositions for modifying a single stranded target nucleic acid
WO2015138510A1 (fr) * 2014-03-10 2015-09-17 Editas Medicine., Inc. Méthodes et compositions associées aux crispr/cas, utilisées dans le traitement de l'amaurose congénitale de leber 10 (lca10)
EP3540061A1 (fr) * 2014-04-02 2019-09-18 Editas Medicine, Inc. Méthodes se rapportant à crispr/cas, et compositions pour traiter le glaucome à angle ouvert primaire
MY181586A (en) * 2014-05-19 2020-12-29 Pfizer Substituted-6,8-dioxabicyclo[3.2.1]octane-2,3-diol compounds as targeting agents of asgpr
EP3204496A1 (fr) * 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions et procédés pour activer une réparation dirigée par homologie
EP3294877A1 (fr) * 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Caractérisation rapide de systèmes d'endonucléases cas, séquences pam et éléments d'arn guide
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
CA3018430A1 (fr) * 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Nouveaux systemes cas et methodes d'utilisation
KR102913446B1 (ko) * 2016-12-14 2026-01-15 바게닝겐 유니버시테이트 열안정성 cas9 뉴클레아제
WO2019051097A1 (fr) * 2017-09-08 2019-03-14 The Regents Of The University Of California Polypeptides de fusion d'endonucléase guidée par arn et procédés d'utilisation correspondants
WO2019123430A1 (fr) * 2017-12-21 2019-06-27 Casebia Therapeutics Llp Substances et méthodes pour le traitement du syndrome d'usher de type 2a et/ou de la rétinite pigmentaire autosomique récessive (arrp) non syndromique
EP3728594A1 (fr) * 2017-12-21 2020-10-28 CRISPR Therapeutics AG Matériaux et méthodes de traitement du syndrome d'usher de type 2a
CN112020554B (zh) * 2018-02-23 2024-10-22 先锋国际良种公司 新颖cas9直系同源物
US12286623B2 (en) * 2018-10-31 2025-04-29 The Regents Of The University Of California Methods and kits for identifying cancer treatment targets

Also Published As

Publication number Publication date
CO2021006938A2 (es) 2021-09-20
CN113785055B (zh) 2025-06-20
CL2021001382A1 (es) 2022-01-07
BR112021010185A2 (pt) 2021-12-28
WO2020111983A3 (fr) 2020-07-23
EP3889269A2 (fr) 2021-10-06
CA3121088A1 (fr) 2020-06-04
JP7698578B2 (ja) 2025-06-25
JP2022513642A (ja) 2022-02-09
MX2021006119A (es) 2021-07-07
PE20212079A1 (es) 2021-10-28
MA53577A1 (fr) 2022-02-28
EP3889269A4 (fr) 2022-08-31
CN113785055A (zh) 2021-12-10
AU2019388420B2 (en) 2025-03-27
WO2020111983A2 (fr) 2020-06-04
PH12021551198A1 (en) 2021-10-25
RU2712497C1 (ru) 2020-01-29
US20220002692A1 (en) 2022-01-06
ZA202103578B (en) 2022-07-27
EA202191504A1 (ru) 2021-09-09
AU2019388420A1 (en) 2021-07-22
KR20210118069A (ko) 2021-09-29

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