MA53577B1 - Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum - Google Patents

Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum

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Publication number
MA53577B1
MA53577B1 MA53577A MA53577A MA53577B1 MA 53577 B1 MA53577 B1 MA 53577B1 MA 53577 A MA53577 A MA 53577A MA 53577 A MA53577 A MA 53577A MA 53577 B1 MA53577 B1 MA 53577B1
Authority
MA
Morocco
Prior art keywords
dna
biotech
valuable
cas9
bacterium clostridium
Prior art date
Application number
MA53577A
Other languages
English (en)
Other versions
MA53577A1 (fr
Inventor
Konstantin Viktorovich Severinov
Sergey Anatolevich Shmakov
Georgii Evgenevich Pobegalov
Aleksandra Andreevna Vasileva
Polina Anatolevna Selkova
Anatolii Nikolaevich Arseniev
Tatyana Igorevna Zyubko
Iana Vitalevna Fedorova
Daria Nikolaevna Artamonova
Ignatiy Igorevich Goryanin
Olga Sergeevna Musharova
Iuliia Valerevna Piskunova
Mikhail Alekseevich Khodorkovskiy
Tatiana Olegovna Artamonova
Marina Viktorovna Abramova
Original Assignee
Biocad Joint Stock Co
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Filing date
Publication date
Application filed by Biocad Joint Stock Co filed Critical Biocad Joint Stock Co
Publication of MA53577A1 publication Critical patent/MA53577A1/fr
Publication of MA53577B1 publication Critical patent/MA53577B1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne une nouvelle nucléase bactérienne du système crispr-cas9 à base de la bactérie clostridium celluloliticum ainsi que son utilisation pour former des ruptures strictement spécifiques à deux brins dans la molécule d'adn. Cette nucléase possède des propriétés inhabituelles et peut s'utiliser en tant qu'instrument pour apporter des changements dans des endroits rigoureusement déterminés dans une séquence d'adn génomique d'organismes monocellullaires ou multicellullaires. De cette manière, on améliore l'universalité des systèmes crispr-cas9 disponibles, ce qui permet d'utiliser des nucléases cas9 provenant d'organismes différents pour découper un adn génomique ou plasmidique dans un plus grand nombre de lieux spécifiques et dans une plus vaste gamme de températures. L'invention permet de simplifier la rédaction du génome de la clostridium celluloliticum ayant une valeur biotechnologique.
MA53577A 2018-11-26 2019-11-26 Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum MA53577B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2018141524A RU2712497C1 (ru) 2018-11-26 2018-11-26 Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum
PCT/RU2019/050229 WO2020111983A2 (fr) 2018-11-26 2019-11-26 Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum

Publications (2)

Publication Number Publication Date
MA53577A1 MA53577A1 (fr) 2022-02-28
MA53577B1 true MA53577B1 (fr) 2022-10-31

Family

ID=69625021

Family Applications (1)

Application Number Title Priority Date Filing Date
MA53577A MA53577B1 (fr) 2018-11-26 2019-11-26 Moyen de découpage d'adn à base de la protéine cas9 sur la base d'une bactérie à valeur biotechnologique clostridium cellulolyticum

Country Status (18)

Country Link
US (1) US20220002692A1 (fr)
EP (1) EP3889269A4 (fr)
JP (1) JP7698578B2 (fr)
KR (1) KR20210118069A (fr)
CN (1) CN113785055B (fr)
AU (1) AU2019388420B2 (fr)
BR (1) BR112021010185A2 (fr)
CA (1) CA3121088A1 (fr)
CL (1) CL2021001382A1 (fr)
CO (1) CO2021006938A2 (fr)
EA (1) EA202191504A1 (fr)
MA (1) MA53577B1 (fr)
MX (1) MX2021006119A (fr)
PE (1) PE20212079A1 (fr)
PH (1) PH12021551198A1 (fr)
RU (1) RU2712497C1 (fr)
WO (1) WO2020111983A2 (fr)
ZA (1) ZA202103578B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3823633A4 (fr) 2018-06-29 2023-05-03 Editas Medicine, Inc. Molécules de guidage synthétiques, compositions et procédés associés
RU2722934C1 (ru) * 2019-06-11 2020-06-05 Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий Средство разрезания днк на основе cas9 белка из бактерии pasteurella pneumotropica

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA115772C2 (uk) * 2011-12-16 2017-12-26 Таргітджин Байотекнолоджиз Лтд Композиція програмованого нуклеопротеїнового молекулярного комплексу і спосіб модифікування заданої послідовності нуклеїнової кислоти-мішені
AU2013266968B2 (en) * 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP2922393B2 (fr) * 2013-02-27 2022-12-28 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Édition de gène dans l'ovocyte au moyen de cas9 nucléases
RU2662932C2 (ru) * 2013-03-14 2018-07-31 Карибо Биосайенсиз, Инк. Композиции и способы с участием нуклеиновых кислот, нацеленных на нуклеиновые кислоты
CA2930877A1 (fr) * 2013-11-18 2015-05-21 Crispr Therapeutics Ag Systeme crips-cas, materiels et procedes
EP3835419A1 (fr) * 2013-12-12 2021-06-16 The Regents of The University of California Procédés et compositions pour modifier un acide nucléique cible monobrin
ES2745769T3 (es) * 2014-03-10 2020-03-03 Editas Medicine Inc Procedimientos y composiciones relacionados con CRISPR/CAS para tratar la amaurosis congénita de Leber 10 (LCA10)
EP3540061A1 (fr) * 2014-04-02 2019-09-18 Editas Medicine, Inc. Méthodes se rapportant à crispr/cas, et compositions pour traiter le glaucome à angle ouvert primaire
PL3145934T3 (pl) * 2014-05-19 2021-08-16 Pfizer Inc. Podstawione związki 6,8-dioksabicyklo[3.2.1]oktano-2,3-diolu jako środki kierujące do ASGPR
AU2015330699B2 (en) * 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
AU2016263026A1 (en) * 2015-05-15 2017-11-09 Pioneer Hi-Bred International, Inc. Guide RNA/Cas endonuclease systems
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
WO2017222773A1 (fr) * 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas et méthodes d'utilisation
BR112019012155A2 (pt) * 2016-12-14 2019-11-12 Wageningen Universiteit uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
US11649442B2 (en) * 2017-09-08 2023-05-16 The Regents Of The University Of California RNA-guided endonuclease fusion polypeptides and methods of use thereof
AU2018393050A1 (en) * 2017-12-21 2020-06-18 Bayer Healthcare Llc Materials and methods for treatment of Usher Syndrome Type 2A
WO2019123430A1 (fr) * 2017-12-21 2019-06-27 Casebia Therapeutics Llp Substances et méthodes pour le traitement du syndrome d'usher de type 2a et/ou de la rétinite pigmentaire autosomique récessive (arrp) non syndromique
WO2019165168A1 (fr) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Nouveaux orthologues de cas9
EP3874045A4 (fr) * 2018-10-31 2022-09-07 The Regents of The University of California Procédés et kits pour identifier des cibles de traitement du cancer

Also Published As

Publication number Publication date
CO2021006938A2 (es) 2021-09-20
PE20212079A1 (es) 2021-10-28
EA202191504A1 (ru) 2021-09-09
WO2020111983A3 (fr) 2020-07-23
PH12021551198A1 (en) 2021-10-25
RU2712497C1 (ru) 2020-01-29
AU2019388420B2 (en) 2025-03-27
EP3889269A4 (fr) 2022-08-31
EP3889269A2 (fr) 2021-10-06
ZA202103578B (en) 2022-07-27
CL2021001382A1 (es) 2022-01-07
US20220002692A1 (en) 2022-01-06
JP7698578B2 (ja) 2025-06-25
CN113785055B (zh) 2025-06-20
CN113785055A (zh) 2021-12-10
BR112021010185A2 (pt) 2021-12-28
CA3121088A1 (fr) 2020-06-04
JP2022513642A (ja) 2022-02-09
AU2019388420A1 (en) 2021-07-22
WO2020111983A2 (fr) 2020-06-04
MX2021006119A (es) 2021-07-07
KR20210118069A (ko) 2021-09-29
MA53577A1 (fr) 2022-02-28

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