MD3781G2 - Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass - Google Patents
Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomassInfo
- Publication number
- MD3781G2 MD3781G2 MDA20080079A MD20080079A MD3781G2 MD 3781 G2 MD3781 G2 MD 3781G2 MD A20080079 A MDA20080079 A MD A20080079A MD 20080079 A MD20080079 A MD 20080079A MD 3781 G2 MD3781 G2 MD 3781G2
- Authority
- MD
- Moldova
- Prior art keywords
- obtaining
- biomass
- spirulina platensis
- preparation
- antioxidant
- Prior art date
Links
- 239000002028 Biomass Substances 0.000 title abstract 4
- 235000016425 Arthrospira platensis Nutrition 0.000 title abstract 3
- 240000002900 Arthrospira platensis Species 0.000 title abstract 3
- 241001464430 Cyanobacterium Species 0.000 title abstract 3
- 230000003078 antioxidant effect Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000002360 preparation method Methods 0.000 title abstract 3
- 239000003963 antioxidant agent Substances 0.000 title abstract 2
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 abstract 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract 1
- 108010012715 Superoxide dismutase Proteins 0.000 abstract 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000006378 damage Effects 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
- 229940032362 superoxide dismutase Drugs 0.000 abstract 1
- 235000002906 tartaric acid Nutrition 0.000 abstract 1
- 239000011975 tartaric acid Substances 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention refers to biotechnology, particularly to a process for obtaining an antioxidant preparation from Spirulina platensis cyanobacterium biomass, used in the pharmaceutical industry and cosmetology.The process, according to the invention, includes cultivation of Spirulina platensis cyanobacterium biomass in open-type reactors on a Gromov culture medium with addition of the coordinative compound with the tartaric acid and imidazole [Mn(COO(CHOH)2COO(C3N2H4)2(H2O)2]·nH2O in the concentration of 20 mg/L at a temperature of 30...35°C, separation of biomass from the culture medium, destruction of cells by repeated congelation-decongelation, extraction of proteic fraction with 0,1 M Na-phosphatic buffer at a pH of 7,8...8,0 with addition of 10 mM EDTA, fractionation of proteic fracture by isoelectric precipitation at a pH of 3,8...4,0, centrifugation with subsequent separation of the precipitate, concentration of the supernatant liquid containing superoxidedismutase and fricocyanine using the Mr 40000 Da polyethylene glycol reagent and congelation.The result consists in obtaining a thermostable preparation with high antioxidant activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20080079A MD3781G2 (en) | 2008-03-18 | 2008-03-18 | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20080079A MD3781G2 (en) | 2008-03-18 | 2008-03-18 | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD3781F1 MD3781F1 (en) | 2008-12-31 |
| MD3781G2 true MD3781G2 (en) | 2009-07-31 |
Family
ID=40255083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20080079A MD3781G2 (en) | 2008-03-18 | 2008-03-18 | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD3781G2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4191C1 (en) * | 2011-12-14 | 2013-07-31 | Государственный Университет Молд0 | Process for extraction of phycocyanin from Spirulina platensis alga biomass |
| MD4320C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Process for determining the amount of Nostoc linckia cyanobacterium biomass |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4319C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Bis[(iminodiacetate)oxothiomolybdate(V)]tetraphenylphosphonium hydrate and process for cultivation of Spirulina platensis cyanobacterium with the use thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5179012A (en) * | 1990-01-11 | 1993-01-12 | Commissariat A L'energie Atomique | Process for the production and extraction of antioxidants from a micro-organism culture |
| US5536654A (en) * | 1993-06-11 | 1996-07-16 | Heliosynthese S.A. Centre D'affaires Actimark Bureau | Process for the production and extraction of thermostable superoxide-dismutases from a photosynthetic microorganism culture |
| MD1008G2 (en) * | 1997-04-30 | 1999-06-30 | Государственный Университет Молд0 | Method for obtaining the phyocyanine |
-
2008
- 2008-03-18 MD MDA20080079A patent/MD3781G2/en not_active IP Right Cessation
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5179012A (en) * | 1990-01-11 | 1993-01-12 | Commissariat A L'energie Atomique | Process for the production and extraction of antioxidants from a micro-organism culture |
| US5536654A (en) * | 1993-06-11 | 1996-07-16 | Heliosynthese S.A. Centre D'affaires Actimark Bureau | Process for the production and extraction of thermostable superoxide-dismutases from a photosynthetic microorganism culture |
| MD1008G2 (en) * | 1997-04-30 | 1999-06-30 | Государственный Университет Молд0 | Method for obtaining the phyocyanine |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4191C1 (en) * | 2011-12-14 | 2013-07-31 | Государственный Университет Молд0 | Process for extraction of phycocyanin from Spirulina platensis alga biomass |
| MD4320C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Process for determining the amount of Nostoc linckia cyanobacterium biomass |
Also Published As
| Publication number | Publication date |
|---|---|
| MD3781F1 (en) | 2008-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chouaib et al. | Towards the standardization of mesenchymal stem cell secretome-derived product manufacturing for tissue regeneration | |
| AU2012274478A8 (en) | Method for amplifying NK cells | |
| WO2007119213A3 (en) | A culture medium and pharmaceutical composition for regenerating cartilage tissue, a method, uses and products related thereto | |
| CA3005308C (en) | Media and fermentation methods for producing polysaccharides in bacterial cell culture | |
| WO2011116922A9 (en) | Thermoreactive substrate with microgels, method for its preparation, and culture method for biological cells | |
| Azzimonti et al. | Microbiota, oxidative stress, and skin cancer: an unexpected triangle | |
| Chang et al. | Exosomes derived from hypoxia-cultured human adipose stem cells alleviate articular chondrocyte inflammaging and post-traumatic osteoarthritis progression | |
| IN2012DN02964A (en) | ||
| WO2009147200A3 (en) | Method for the supply of growth components to cell cultures | |
| Yoon et al. | Procollagen C-endopeptidase enhancer 2 secreted by tonsil-derived mesenchymal stem cells increases the oxidative burst of promyelocytic HL-60 cells | |
| MD3781G2 (en) | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass | |
| Müller et al. | Polyphosphate reverses the toxicity of the quasi-enzyme Bleomycin on alveolar endothelial lung cells in vitro | |
| MD3417G2 (en) | Nutrient medium for cultivation of Spirulina platensis cyanobacyterium | |
| Jo et al. | Therapeutic Potential of Stem Cell-Derived Exosomes in Skin Wound Healing | |
| WO2009035612A3 (en) | Multilineage-inducible cells and uses thereof | |
| Roato et al. | Suppressing the Aging Phenotype of Mesenchymal Stromal Cells: Are We Ready for Clinical Translation? | |
| CN110669724A (en) | Saccharum embryo culture solution and preparation method thereof | |
| MD3129G2 (en) | Process for Spirulina platensis cyanobacterium biomass obtaining | |
| NZ596746A (en) | Blood-derived supplement with platelets activator for cell cultivation media | |
| MD4714B1 (en) | Process for cultivation of Spirulina platensis cyanobacterium | |
| MD3128G2 (en) | Process for Spirulina platensis biomass obtaining | |
| WO2013129978A3 (en) | Nano-antibody, specifically binding muc1 protein, and method for detecting muc1 protein with the aid of the nano-antibodies | |
| MD191Z5 (en) | Nutrient medium for the cultivation of cells of animal origin | |
| CN104450670A (en) | Method for improving enzyme-producing activity of host cells | |
| EP4464777A1 (en) | Method for increasing exosome productivity and composition comprising exosomes produced thereby |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |