MD4122C1 - Strain of Spirulina platensis (Nordst) Geitl alga as a source of biologically active substances - Google Patents
Strain of Spirulina platensis (Nordst) Geitl alga as a source of biologically active substances Download PDFInfo
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- MD4122C1 MD4122C1 MDA20110016A MD20110016A MD4122C1 MD 4122 C1 MD4122 C1 MD 4122C1 MD A20110016 A MDA20110016 A MD A20110016A MD 20110016 A MD20110016 A MD 20110016A MD 4122 C1 MD4122 C1 MD 4122C1
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- geitl
- nordst
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- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 15
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 15
- 239000013543 active substance Substances 0.000 title claims abstract description 6
- 241000195493 Cryptophyta Species 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 abstract description 13
- 229920001282 polysaccharide Polymers 0.000 abstract description 13
- 239000005017 polysaccharide Substances 0.000 abstract description 13
- 150000003905 phosphatidylinositols Chemical class 0.000 abstract description 12
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 abstract description 10
- 244000005700 microbiome Species 0.000 abstract description 2
- 235000020778 linoleic acid Nutrition 0.000 abstract 2
- 235000013922 glutamic acid Nutrition 0.000 description 12
- 239000002028 Biomass Substances 0.000 description 10
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 10
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 10
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 10
- 239000004220 glutamic acid Substances 0.000 description 10
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 description 8
- 229960002733 gamolenic acid Drugs 0.000 description 8
- 230000000975 bioactive effect Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- 229940082787 spirulina Drugs 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- LMSDCGXQALIMLM-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;iron Chemical compound [Fe].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O LMSDCGXQALIMLM-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 2
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 2
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 235000013734 beta-carotene Nutrition 0.000 description 2
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 2
- 239000011648 beta-carotene Substances 0.000 description 2
- 229960002747 betacarotene Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229910052927 chalcanthite Inorganic materials 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000011565 manganese chloride Substances 0.000 description 2
- 235000002867 manganese chloride Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000007003 mineral medium Substances 0.000 description 2
- 229930195732 phytohormone Natural products 0.000 description 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000010344 sodium nitrate Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000010384 tocopherol Nutrition 0.000 description 2
- 229960001295 tocopherol Drugs 0.000 description 2
- 229930003799 tocopherol Natural products 0.000 description 2
- 239000011732 tocopherol Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 description 2
- 239000011686 zinc sulphate Substances 0.000 description 2
- 235000009529 zinc sulphate Nutrition 0.000 description 2
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 2
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241001464430 Cyanobacterium Species 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004112 neuroprotection Effects 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 108060006184 phycobiliprotein Proteins 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Chemical group 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Chemical group 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
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Abstract
Description
Invenţia se referă la biotehnologie şi poate fi utilizată pentru obţinerea substanţelor biologic active. The invention refers to biotechnology and can be used to obtain biologically active substances.
Biomasa de spirulină oferă un spectru deplin al aminoacizilor, prezenţi atât în stare liberă, cât şi legată. Un interes practic major îl prezintă aminoacizii imunoactivi, care joacă un rol esenţial în procesele de protecţie a organismului. Pe lângă cele menţionate, acidul glutamic mai posedă şi funcţia de neuroprotecţie. Polizaharidele sulfatate prezente în biomasa de spirulină manifestă activitate antitumorală, antivirală şi imunostimulatoare. Fracţia fosfolipidelor esenţiale include şi fosfatidilinozitolul, care este recunoscut în calitate de normolipemiant şi cardioprotector. Un alt component bioactiv din spirulină este acidul gras polinesaturat γ-linolenic, care se caracterizează prin acţiune antieterogenă pronunţată. Spirulina biomass offers a full spectrum of amino acids, present both in free and bound state. Of major practical interest are the immunoactive amino acids, which play an essential role in the body's protection processes. In addition to the mentioned, glutamic acid also has the function of neuroprotection. The sulfated polysaccharides present in spirulina biomass exhibit antitumor, antiviral and immunostimulating activity. The fraction of essential phospholipids also includes phosphatidylinositol, which is recognized as a normolipemiant and cardioprotector. Another bioactive component of spirulina is the γ-linolenic polyunsaturated fatty acid, which is characterized by pronounced antiheterogeneous action.
Este cunoscută tulpina Spirulina platensis (Nordst) Geitl, CALU-835. Biomasa absolut uscată a acestei tulpini conţine 65…70% proteine, 10…15% hidraţi de carbon, 3…5% lipide, 0,42…0,44% β-carotină, 80…100 mg/% acid ascorbic, 30…35 mg/% tocoferol, vitamine din grupa B, 0,36% acid glutamic, fitohormoni şi alte substanţe biologic active [1]. The strain Spirulina platensis (Nordst) Geitl, CALU-835, is known. The absolutely dry biomass of this strain contains 65...70% proteins, 10...15% carbohydrates, 3...5% lipids, 0.42...0.44% β-carotene, 80...100 mg/% ascorbic acid, 30... 35 mg/% tocopherol, group B vitamins, 0.36% glutamic acid, phytohormones and other biologically active substances [1].
Mai este cunoscută tulpina Spirulina platensis (Nordst) Geitl, CNM-CB-01, cu biomasa absolut uscată, care conţine: 1,3…3,3% aminoacizi liberi, 5,5…6,5% oligopeptide, 62…70% proteine, 9…11% ficobiliproteide, 10…15% polizaharide, 3…5% lipide, 0,42…0,44% β-carotină, 30…35 mg/% tocoferol, 80…100 mg/% acid ascorbic, vitaminele grupei B, 1,0…1,2% acid γ-linolenic; 6,0…6,3% acid glutamic, fitohormoni şi alte substanţe bioactive [2]. Also known is the strain Spirulina platensis (Nordst) Geitl, CNM-CB-01, with absolutely dry biomass, which contains: 1.3...3.3% free amino acids, 5.5...6.5% oligopeptides, 62...70% proteins, 9...11% phycobiliproteins, 10...15% polysaccharides, 3...5% lipids, 0.42...0.44% β-carotene, 30...35 mg/% tocopherol, 80...100 mg/% ascorbic acid, vitamins group B, 1.0...1.2% γ-linolenic acid; 6.0...6.3% glutamic acid, phytohormones and other bioactive substances [2].
Dezavantajele tulpinilor menţionate mai sus constau în conţinutul redus al compuşilor bioactivi de interes major, aşa ca acidul glutamic, acidul γ-linolenic, fosfatidilinozitolul şi polizaharidele sulfatate. Valorile acestor indici sunt date în tabel. The disadvantages of the strains mentioned above consist in the low content of bioactive compounds of major interest, such as glutamic acid, γ-linolenic acid, phosphatidylinositol and sulfated polysaccharides. The values of these indices are given in the table.
Tabel Table
Conţinutul unor substanţe bioactive în biomasa a două tulpini de spirulină, % biomasă absolut uscată The content of some bioactive substances in the biomass of two spirulina strains, % absolute dry biomass
Tulpina Acidul glutamic Acidul γ-linolenic Fosfatidilinozitolul Fosfatidil-colină Polizaharide sulfatate Spirulina platensis (Nordst) Geitl, CALU-835 Date din literatură 0,36 - - - - Indici estimaţi 0,34…0,42 0,54…0,60 0,45…0,48 0,94…1,05 1,12…1,36 Spirulina platensis (Nordst) Geitl CNM-CB-01 Date din literatură 6,00…6,30 1,00…1,20 - - - Indici estimaţi 4,70…6,40 1,15…1,22 0,60…0,73 1,35…1,62 2,33…3,45 Strain Glutamic acid γ-linolenic acid Phosphatidylinositol Phosphatidyl-choline Sulfated polysaccharides Spirulina platensis (Nordst) Geitl, CALU-835 Literature data 0.36 - - - - Estimated indices 0.34...0.42 0.54...0.60 0 .45…0.48 0.94…1.05 1.12…1.36 Spirulina platensis (Nordst) Geitl CNM-CB-01 Literature data 6.00…6.30 1.00…1.20 - - - Estimated indices 4.70...6.40 1.15...1.22 0.60...0.73 1.35...1.62 2.33...3.45
Problema pe care o rezolvă prezenta invenţie constă în obţinerea unei tulpini noi de Spirulina platensis, biomasa căreia s-ar caracteriza printr-un conţinut înalt de substanţe biologic active, în special de acid glutamic, acid γ-linolenic, fosfatidilinozitol, fosfatidilcolină şi polizaharide sulfatate. The problem that the present invention solves consists in obtaining a new strain of Spirulina platensis, the biomass of which would be characterized by a high content of biologically active substances, especially glutamic acid, γ-linolenic acid, phosphatidylinositol, phosphatidylcholine and sulfated polysaccharides .
Esenţa invenţiei constă în faptul că se propune o tulpină de algă Spirulina platensis (Nordst) Geitl care este depozitată în Colecţia Naţională de Microorganisme Nepatogene a Republicii Moldova a Institutului de Microbiologie şi Biotehnologie al AŞM cu numărul CNMN-CB-11 şi poate fi utilizată în calitate de sursă de acizi glutamic şi γ-linolenic, polizaharide sulfatate, fosfatidilinozitol şi fosfatidilcolină. The essence of the invention consists in the fact that a strain of Spirulina platensis (Nordst) Geitl algae is proposed which is stored in the National Collection of Non-pathogenic Microorganisms of the Republic of Moldova of the Institute of Microbiology and Biotechnology of AŞM with the number CNMN-CB-11 and can be used in as a source of glutamic and γ-linolenic acids, sulfated polysaccharides, phosphatidylinositol and phosphatidylcholine.
Rezultatul constă în sporirea productivităţii algei şi a cantităţii de acizi glutamic şi γ-linolenic, polizaharide sulfatate, fosfatidilinozitol şi fosfatidilcolină. The result consists in increasing the productivity of the algae and the amount of glutamic and γ-linolenic acids, sulfated polysaccharides, phosphatidylinositol and phosphatidylcholine.
Rezultatul tehnic al invenţiei, în comparaţie cu cea mai apropiată soluţie, constă în sporirea conţinutului a cinci componente bioactive de bază în biomasa absolut uscată a cianobacteriei: acid glutamic 8,10…9,64% (faţă de 4,70…6,40% la cea mai apropiată soluţie); acid γ-linolenic 1,36…1,45% (faţă de 1,15…1,22% la cea mai apropiată soluţie); polizaharide sulfatate 4,03…5,01% (faţă de 2,33…3,45% la cea mai apropiată soluţie); fosfatidilinozitol 0,70…0,85% (faţă de 0,60…0,73% la cea mai apropiată soluţie) şi fosfatidilcolină 1,86…2,00% (faţă de 1,35…l,62% la cea mai apropiată soluţie). The technical result of the invention, compared to the closest solution, consists in increasing the content of five basic bioactive components in the absolutely dry biomass of the cyanobacterium: glutamic acid 8.10...9.64% (compared to 4.70...6.40 % to the closest solution); γ-linolenic acid 1.36...1.45% (compared to 1.15...1.22% for the closest solution); sulfated polysaccharides 4.03...5.01% (compared to 2.33...3.45% for the closest solution); phosphatidylinositol 0.70...0.85% (compared to 0.60...0.73% at the closest solution) and phosphatidylcholine 1.86...2.00% (compared to 1.35...l.62% at the closest approximate solution).
Rezultatul tehnic obţinut se datorează particularităţilor fiziologice şi biochimice ale tulpinii, în special proceselor biosintetice, care decurg în acest organism. Ca rezultat al adaptării tulpinii la o temperatură mai joasă de cultivare are loc modificarea ciclurilor biosintetice cu deplasarea lor spre produsele nominalizate. The technical result obtained is due to the physiological and biochemical particularities of the strain, especially the biosynthetic processes that take place in this organism. As a result of the adaptation of the strain to a lower cultivation temperature, the modification of the biosynthetic cycles takes place with their displacement towards the nominated products.
Tulpina propusă a fost colectată dintr-un bazin acvatic artificial din apropierea satului Căpriana. The proposed strain was collected from an artificial water basin near the village of Căpriana.
În cultura algologic pură tulpina se multiplică intens pe mediul mineral lichid cu următoarea componenţă a macroelementelor, g/L: NaNO3 - 2,25; NaHCO3 - 8,0; NaCl - 1,01; K2SO4 - 0,3; NaHPO4 - 0,20; MgSO3·7H2O - 0,20; CaCl2 - 0,024; soluţia de microelemente 1 mL/L, ce conţine în mg/L: H3BO3 - 2,86; MnCl2·4H2O - 1,81; ZnSO4·7H2O - 0,22; CuSO4·5H2O - 0,08; MoO3 - 0,015; Fe-EDTA - l,0 mL/L. In the pure algal culture, the strain multiplies intensively on the liquid mineral medium with the following composition of macroelements, g/L: NaNO3 - 2.25; NaHCO3 - 8.0; NaCl - 1.01; K2SO4 - 0.3; NaHPO4 - 0.20; MgSO3·7H2O - 0.20; CaCl2 - 0.024; microelement solution 1 mL/L, which contains in mg/L: H3BO3 - 2.86; MnCl2·4H2O - 1.81; ZnSO4·7H2O - 0.22; CuSO4·5H2O - 0.08; MoO3 - 0.015; Fe-EDTA - l.0 mL/L.
În condiţii optime de cultivare tulpina respectivă are o productivitate de 1,5…1,6 g/L cu conţinut de acid glutamic 8,10…9,64%; acid γ-linolenic 1,36…1,45%; polizaharide sulfatate 4,03…5,01%, fosfatidilinozitol 0,70…0,85%, şi fosfatidilcolină 1,86…2,00%. In optimal cultivation conditions, the respective strain has a productivity of 1.5...1.6 g/L with a glutamic acid content of 8.10...9.64%; γ-linolenic acid 1.36...1.45%; sulfated polysaccharides 4.03...5.01%, phosphatidylinositol 0.70...0.85%, and phosphatidylcholine 1.86...2.00%.
Caracterele morfo - culturale ale tulpinii Morpho-cultural characters of the strain
Tulpina prezintă forme filamentoase homocite de culoare verde închisă. Trihomele sunt alcătuite din celule cilindrice (lungimea 8…10 µm, lăţimea 6…8 µm) absolut identice, cu excepţia celulelor terminale, care se îngustează spre capătul trihomului şi sunt uşor rotunjite. Creşterea lor are loc ca rezultat al diviziunii transversale a celulelor. Celulele conţin granule, situate în apropierea pereţilor celulari. The stem shows dark green homocytic filamentous forms. The trichomes are made up of cylindrical cells (length 8...10 µm, width 6...8 µm) absolutely identical, except for the terminal cells, which narrow towards the end of the trichome and are slightly rounded. Their growth occurs as a result of the transverse division of cells. The cells contain granules, located near the cell walls.
Trihomele sunt separate, nu se ramifică şi nu se răsucesc în formă de spirală. Diametrul trihomei este de 6…8 µm, iar lungimea de 0,14 …1,30 mm. Ele nu formează teci mucozitare. The trichomes are separate, do not branch and do not twist in a spiral shape. The diameter of the trichome is 6...8 µm, and the length is 0.14...1.30 mm. They do not form mucocytic sheaths.
În mediu lichid formează suspensie practic uniformă cu tendinţă neînsemnată de acumulare la hotarul lichid-aer. In a liquid environment, it forms a practically uniform suspension with an insignificant tendency to accumulate at the liquid-air boundary.
Caracterele fiziologo - biochimice Physiological - biochemical characters
Tulpina microalgei verzi Spirulina platensis (Nordst) GEITL CNMN-CB-11 creşte bine pe mediul SP1, componenţa căruia a fost menţionată mai sus. The green microalga strain Spirulina platensis (Nordst) GEITL CNMN-CB-11 grows well on the SP1 medium, the composition of which was mentioned above.
Temperatura optimă este de 26±2°C, pH 8…9. Intensitatea optimă a luminii este de 1500…2000 lx. The optimal temperature is 26±2°C, pH 8...9. The optimal light intensity is 1500...2000 lx.
Tulpina se adaptează uşor la variaţii neînsemnate ale condiţiilor de cultivare. The strain easily adapts to insignificant variations in cultivation conditions.
În condiţii optime de cultivare tulpina respectivă are o productivitate de 1,5…1,6 g/L cu conţinut de acid glutamic 8,10…9,64%; acid γ-linolenic 1,36…1,45%; polizaharide sulfatate 4,03…5,01%, fosfatidilinozitol 0,70…0,85% şi fosfatidilcolină 1,86…2,00%. In optimal cultivation conditions, the respective strain has a productivity of 1.5...1.6 g/L with a glutamic acid content of 8.10...9.64%; γ-linolenic acid 1.36...1.45%; sulfated polysaccharides 4.03...5.01%, phosphatidylinositol 0.70...0.85% and phosphatidylcholine 1.86...2.00%.
Gradul de puritate a tulpinii The degree of purity of the strain
Tulpina a fost obţinută prin metoda "pasaje repetate" în cultura algologic pură şi acumulată în mediul lichid şi agarizat SP1. La cultivarea în masă poate fi infectată cu alge şi cianobacterii. Contaminarea poate fi evitată prin respectarea condiţiilor de alcalinitate a mediului de cultură. The strain was obtained by the "repeated passages" method in pure algal culture and accumulated in SP1 liquid and agar medium. In mass cultivation it can be infected with algae and cyanobacteria. Contamination can be avoided by respecting the alkalinity conditions of the culture medium.
Exemplu de realizare a invenţiei Example of realization of the invention
În vasele de cultură cu un volum de 1 L cu mediu mineral lichid al macro- şi microelementelor: macroelemente cu următoarea componenţă, g/L: NaNO3 - 2,25; NaHCO3 - 8,0; NaCl - 1,01; K2SO4 - 0,3; NaHPO4 - 0,20; MgSO3·7H2O - 0,20; CaCl2 - 0,024; microelemente - 1 mL/L, cu următoarea componenţă, mg/L: Н3ВО3 - 2,86; MnCl2·4H2O - 1,81; ZnSO4·7H2O - 0,22; CuSO4·5H2O - 0,08; MoO3 - 0,015; Fe-EDTA - l,0 mL/L, pregătit pe apă distilată, se introduce inoculum de 0,40…0,45 g/L în recalcul la biomasa absolut uscată. Cultivarea se realizează la intensitatea luminii de 1500…2000 lx timp de 6 zile, la temperatura de 26±1°C, în ziua a 6-ea productivitatea ajunge până la 1,6 g/L biomasă absolut uscată, cu conţinut de acid glutamic 9,3%; acid γ-linolenic 1,39%; polizaharide sulfatate 4,76%, fosfatidilinozitol 0,79% şi fosfatidilcolină 1,94%. In culture vessels with a volume of 1 L with liquid mineral medium of macro- and microelements: macroelements with the following composition, g/L: NaNO3 - 2.25; NaHCO3 - 8.0; NaCl - 1.01; K2SO4 - 0.3; NaHPO4 - 0.20; MgSO3·7H2O - 0.20; CaCl2 - 0.024; trace elements - 1 mL/L, with the following composition, mg/L: Н3ВО3 - 2.86; MnCl2·4H2O - 1.81; ZnSO4·7H2O - 0.22; CuSO4·5H2O - 0.08; MoO3 - 0.015; Fe-EDTA - 1.0 mL/L, prepared on distilled water, introduce inoculum of 0.40...0.45 g/L in recalculation to absolutely dry biomass. Cultivation is carried out at a light intensity of 1500...2000 lx for 6 days, at a temperature of 26±1°C, on the 6th day the productivity reaches up to 1.6 g/L absolutely dry biomass, with glutamic acid content 9.3%; γ-linolenic acid 1.39%; sulfated polysaccharides 4.76%, phosphatidylinositol 0.79% and phosphatidylcholine 1.94%.
1. MD 169 C2 1995.03.31 1. MD 169 C2 1995.03.31
2. Rudic V., Rudi L., Cepoi L. Aspecte biotehnologice de reglare a biosintezei acizilor graşi polienici la Spirulina platensis CNM-CB-01. Buletinul Academiei de Ştiinţe a Moldovei. Seria Ştiinţele vieţii. Chişinău, 2007, nr.1 (301), p. 135-142 2. Rudic V., Rudi L., Cepoi L. Biotechnological aspects of regulating the biosynthesis of polyenic fatty acids in Spirulina platensis CNM-CB-01. Bulletin of the Academy of Sciences of Moldova. Life Sciences Series. Chisinau, 2007, no. 1 (301), p. 135-142
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4334C1 (en) * | 2014-02-03 | 2015-10-31 | Государственный Университет Молд0 | Strain of blue-green microalga Cylindrospermum licheniforme (Bory) Kütz - source of glucides |
| MD4351C1 (en) * | 2014-04-08 | 2016-01-31 | Государственный Университет Молд0 | Strain of blue-green microalga Calothrix elenkinii Kossinsk. - source of glucides |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD4319C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Bis[(iminodiacetate)oxothiomolybdate(V)]tetraphenylphosphonium hydrate and process for cultivation of Spirulina platensis cyanobacterium with the use thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD169C2 (en) * | 1985-04-10 | 1995-07-31 | Государственный Университет Молд0 | The stem of alga Spirulina platensis (Nordst) Geitl CALU - biomass producer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD169C2 (en) * | 1985-04-10 | 1995-07-31 | Государственный Университет Молд0 | The stem of alga Spirulina platensis (Nordst) Geitl CALU - biomass producer |
Non-Patent Citations (1)
| Title |
|---|
| Rudic V., Rudi L., Cepoi L. Aspecte biotehnologice de reglare a biosintezei acizilor graşi polienici la Spirulina platensis CNM-CB-01. Buletinul Academiei de Ştiinţe a Moldovei. Seria Ştiinţele vieţii. Chişinău, 2007, nr.1 (301), p. 135-142 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4334C1 (en) * | 2014-02-03 | 2015-10-31 | Государственный Университет Молд0 | Strain of blue-green microalga Cylindrospermum licheniforme (Bory) Kütz - source of glucides |
| MD4351C1 (en) * | 2014-04-08 | 2016-01-31 | Государственный Университет Молд0 | Strain of blue-green microalga Calothrix elenkinii Kossinsk. - source of glucides |
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| MD4122B1 (en) | 2011-07-31 |
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| PD4A | Change of proprietorship (patent for invention) |
Owner name: "VIORICA-COSMETIC" S.A., MD STR. MESAGER NR.1, MD- Free format text: PREVIOUS PROPRIETOR: RUDIC VALERIU, MD |