MX2009010331A - Marcador de celula germinal utilizando el gen vasa de pescado. - Google Patents

Marcador de celula germinal utilizando el gen vasa de pescado.

Info

Publication number
MX2009010331A
MX2009010331A MX2009010331A MX2009010331A MX2009010331A MX 2009010331 A MX2009010331 A MX 2009010331A MX 2009010331 A MX2009010331 A MX 2009010331A MX 2009010331 A MX2009010331 A MX 2009010331A MX 2009010331 A MX2009010331 A MX 2009010331A
Authority
MX
Mexico
Prior art keywords
fish
germ cell
tuna
recipient
vasa
Prior art date
Application number
MX2009010331A
Other languages
English (en)
Inventor
Goro Yoshizaki
Yutaka Takeuchi
Kazue Nagasawa
Kentaro Higuchi
Tetsuro Morita
Naoki Kabeya
Original Assignee
Univ Tokyo Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Tokyo Nat Univ Corp filed Critical Univ Tokyo Nat Univ Corp
Publication of MX2009010331A publication Critical patent/MX2009010331A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Con el objeto de examinar si o no una célula germinal derivada de un pez donante, la cual ha sido transplantada dentro de un pez recipiente de una especie diferente mediante una técnica de pez sustituto, crece o madura en la gónada del pez recipiente, es necesario utilizar, como un indicador, un rasgo que es específicamente expresado en la célula germinal y que se puede utilizar para distinguir el pez recipiente del pez donante; el gen Vasa, el cual es un gen especifico de la célula germinal, es específico a una célula germinal primordial y a un espermatogonio/un ovogonio, y no se expresa en una célula somática; en la presente invención, se determinaron las secuencias del gen Vasa de un atún, una macarela chub, una macarela manchada, un atún oriental pequeño, y un pez tambor, y la expresión de dicho gen se utiliza como un marcador para una célula germinal; además, de acuerdo con la presente invención, es posible detectar específicamente solamente un gen Vasa del atún en las secuencias del gen Vasa que están altamente conservadas en los peces, sin secuenciación; por lo tanto, una célula germinal derivada de atún se puede identificar de manera confiable y simple en la gónada del pez recipiente; como un resultado, el crecimiento o reproducción del atún se puede llevar a cabo con buena eficiencia; además, utilizando los hallazgos anteriormente mencionados, incluso en un caso en el cual no solamente un atún sino que también otros peces Perciformes se utilizan como un donante, se puede detectar eficientemente una célula germinal derivada del pez donante a partir de la gónada de un pez recipiente de una especie diferente.
MX2009010331A 2007-03-26 2008-03-26 Marcador de celula germinal utilizando el gen vasa de pescado. MX2009010331A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007080022 2007-03-26
PCT/JP2008/000743 WO2008129838A1 (ja) 2007-03-26 2008-03-26 魚類のＶａｓａ遺伝子を用いた生殖細胞マーカー

Publications (1)

Publication Number Publication Date
MX2009010331A true MX2009010331A (es) 2009-10-16

Family

ID=39875370

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009010331A MX2009010331A (es) 2007-03-26 2008-03-26 Marcador de celula germinal utilizando el gen vasa de pescado.

Country Status (9)

Country Link
US (1) US8222385B2 (es)
EP (1) EP2133423B1 (es)
JP (1) JP5509417B2 (es)
KR (1) KR20100014457A (es)
AU (1) AU2008241974B2 (es)
ES (1) ES2401724T3 (es)
IL (1) IL201182A0 (es)
MX (1) MX2009010331A (es)
WO (1) WO2008129838A1 (es)

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Also Published As

Publication number Publication date
KR20100014457A (ko) 2010-02-10
ES2401724T3 (es) 2013-04-24
US8222385B2 (en) 2012-07-17
EP2133423A4 (en) 2010-04-07
AU2008241974A1 (en) 2008-10-30
JP2008263967A (ja) 2008-11-06
AU2008241974B2 (en) 2013-09-12
EP2133423A1 (en) 2009-12-16
IL201182A0 (en) 2011-08-01
JP5509417B2 (ja) 2014-06-04
EP2133423B1 (en) 2013-01-09
WO2008129838A1 (ja) 2008-10-30
US20100132056A1 (en) 2010-05-27

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