MX2012002902A - Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado. - Google Patents
Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado.Info
- Publication number
- MX2012002902A MX2012002902A MX2012002902A MX2012002902A MX2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- amplification
- acid amplification
- compensation
- spectral
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 8
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 8
- 150000007523 nucleic acids Chemical class 0.000 title abstract 8
- 102000039446 nucleic acids Human genes 0.000 title abstract 8
- 108020004707 nucleic acids Proteins 0.000 title abstract 8
- 230000003595 spectral effect Effects 0.000 title abstract 4
- 239000000523 sample Substances 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Evolutionary Biology (AREA)
- Theoretical Computer Science (AREA)
- Medical Informatics (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Algebra (AREA)
- General Physics & Mathematics (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
Abstract
La presente invención se refiere a un método que incluye realizar una ampliación de ácido nucleico de una muestra de ácido nucleico usando una sonda de detección, en donde la amplificación de ácido nucleico ocurre sobre uno o más periodos de interrogación, y, de la amplificación de ácido nucleico, adquirir datos de amplificación que indican una cantidad de ácido nucleico presente en cada uno de uno o más periodos de interrogación. El método también incluye, en base a los datos de amplificación, determinar un valor de corrección de diafonía asociado con un vecino espectral a la sonda para reducir la diafonía espectral del vecino espectral; y aplicar el valor de corrección de diafonía a los datos de amplificación recolectados de amplificaciones de ácido nucleico multiplexados de muestras de ácido nucleico.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24130709P | 2009-09-10 | 2009-09-10 | |
| US35828710P | 2010-06-24 | 2010-06-24 | |
| PCT/US2010/047265 WO2011031585A1 (en) | 2009-09-10 | 2010-08-31 | Compensation for spectral crosstalk in multiplex nucleic acid amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2012002902A true MX2012002902A (es) | 2012-04-02 |
| MX352971B MX352971B (es) | 2017-12-15 |
Family
ID=43732754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012002902A MX352971B (es) | 2009-09-10 | 2010-08-31 | Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado. |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US9938569B2 (es) |
| EP (3) | EP3375891B1 (es) |
| CN (1) | CN102482720B (es) |
| DK (3) | DK2475788T3 (es) |
| ES (3) | ES2791369T3 (es) |
| MX (1) | MX352971B (es) |
| PT (3) | PT3375891T (es) |
| WO (1) | WO2011031585A1 (es) |
Families Citing this family (26)
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| AU2009240461A1 (en) | 2008-04-24 | 2009-10-29 | 3M Innovative Properties Company | Analysis of nucleic acid amplification curves using wavelet transformation |
| DK2475788T3 (en) * | 2009-09-10 | 2018-07-30 | Diasorin S P A | COMPENSATION FOR SPECTRAL CRYSTAL SAMPLES IN MULTIPLEX NUCLEIC ACID AMPLIFICATION |
| CN107326085B (zh) | 2012-02-03 | 2022-05-03 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| KR101969846B1 (ko) | 2012-06-28 | 2019-04-17 | 삼성전자주식회사 | Ｐｃｒ 데이터 및 다른 데이터에서 크로스토크의 영향을 보상하여 핵산을 분석하는 방법 및 장치 |
| IN2015DN01609A (es) | 2012-08-03 | 2015-07-03 | California Inst Of Techn | |
| US8858886B1 (en) | 2013-05-08 | 2014-10-14 | Agilent Technologies, Inc. | Scanning system with interchangeable optical cartridges for fluorescence measurements |
| US9170198B2 (en) * | 2013-12-19 | 2015-10-27 | Luminex Corporation | Crosstalk reduction |
| BE1022048B1 (nl) * | 2014-04-11 | 2016-02-10 | Ugentec Bvba | Methoden voor fluorescentie data correctie |
| ES2899739T3 (es) * | 2014-08-27 | 2022-03-14 | Hoffmann La Roche | Un procedimiento de análisis y sistema para analizar una reacción de amplificación de ácido nucleico |
| CN107426044B (zh) * | 2016-05-23 | 2022-06-07 | 中兴通讯股份有限公司 | 一种串线检测方法、装置及操作维护服务器 |
| US10066263B2 (en) | 2016-06-17 | 2018-09-04 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| WO2018077739A1 (en) * | 2016-10-25 | 2018-05-03 | Qiagen Gmbh | Cross-talk compensation |
| US10623132B2 (en) * | 2018-03-06 | 2020-04-14 | J3 Technology LLC | Barrage jammer with contoured amplitude |
| CN112424377A (zh) | 2018-04-17 | 2021-02-26 | 克罗玛科德公司 | 用于多重分析的方法和系统 |
| CN108841939B (zh) * | 2018-06-21 | 2020-09-22 | 北京致雨生物科技有限公司 | 多重数字pcr浓度测量方法及微滴式数字pcr系统 |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| CN116419977A (zh) * | 2020-07-23 | 2023-07-11 | 生命技术公司 | 用于生物分析的系统和方法 |
| CN111849758A (zh) * | 2020-08-18 | 2020-10-30 | 上海基灵生物科技有限公司 | 全自动实时荧光定量pcr检测系统 |
| CN113174326B (zh) * | 2021-01-15 | 2021-12-10 | 北京中科生仪科技有限公司 | 基于pcr的纯化扩增反应中的控制装置 |
| CN114752663B (zh) * | 2022-05-18 | 2024-04-30 | 苏州国科芯感医疗科技有限公司 | 一种荧光串扰校正方法及其应用 |
| US12510480B2 (en) * | 2023-03-31 | 2025-12-30 | Araceli Biosciences Inc. | Systems and methods for fluorescence microscopy channel crosstalk mitigation |
| EP4481058A1 (en) * | 2023-06-23 | 2024-12-25 | QIAGEN GmbH | Method, use and device for signal collection in a process utilizing fluorescence at different wavelengths |
| WO2025012430A1 (en) * | 2023-07-13 | 2025-01-16 | Curetis Gmbh | Radiation transfer unit and device comprising the same |
| CN117434044B (zh) * | 2023-12-19 | 2024-03-08 | 鲲鹏基因（北京）科技有限责任公司 | 一种pcr仪荧光串扰系数标定方法及装置与应用 |
| CN118010685A (zh) * | 2023-12-22 | 2024-05-10 | 深圳市真迈生物科技有限公司 | 一种确定通道间串扰校正强度的方法、装置及介质 |
| WO2025230707A1 (en) * | 2024-05-02 | 2025-11-06 | Gen-Probe Incorporated | Adaptive crosstalk compensation of optical signal detection |
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| US7133726B1 (en) | 1997-03-28 | 2006-11-07 | Applera Corporation | Thermal cycler for PCR |
| US6303305B1 (en) | 1999-03-30 | 2001-10-16 | Roche Diagnostics, Gmbh | Method for quantification of an analyte |
| US6156465A (en) | 1999-04-12 | 2000-12-05 | Cymbolic Sciences Inc. | Crosstalk correction |
| US6333501B1 (en) | 2000-01-27 | 2001-12-25 | Perkin-Elmer Corporation | Methods, apparatus, and articles of manufacture for performing spectral calibration |
| US6734401B2 (en) | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| US7188030B2 (en) | 2001-08-21 | 2007-03-06 | Applera Corporation | Automatic threshold setting for quantitative polymerase chain reaction |
| US7435602B2 (en) | 2002-12-20 | 2008-10-14 | Applied Biosystems Inc. | Reducing effects of spectral nonuniformity |
| US7788039B2 (en) * | 2003-09-25 | 2010-08-31 | Roche Molecular Systems, Inc. | Quantitation of nucleic acids using growth curves |
| US7177023B2 (en) | 2004-03-19 | 2007-02-13 | Applera Corporation | Fluorescent light detection |
| US7023451B2 (en) | 2004-06-14 | 2006-04-04 | Sharp Laboratories Of America, Inc. | System for reducing crosstalk |
| US7045756B2 (en) | 2004-08-05 | 2006-05-16 | Applera Corporation | Methods and systems for in situ calibration of imaging in biological analysis |
| US20060051796A1 (en) * | 2004-09-09 | 2006-03-09 | Inga Boell | Real time PCR with the addition of pyrophosphatase |
| US7709249B2 (en) * | 2005-04-01 | 2010-05-04 | 3M Innovative Properties Company | Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector |
| US7507575B2 (en) * | 2005-04-01 | 2009-03-24 | 3M Innovative Properties Company | Multiplex fluorescence detection device having removable optical modules |
| CN101156059B (zh) * | 2005-04-01 | 2011-06-08 | 3M创新有限公司 | 具有将多个光学模块连接到公共检测器的纤维束的多重荧光检测装置 |
| EP1891578A2 (en) | 2005-06-15 | 2008-02-27 | Gen-Probe Incorporated | Methods for quantitative analysis of a nucleic acid amplification reaction |
| US20060291706A1 (en) | 2005-06-23 | 2006-12-28 | Applera Corporation | Method of extracting intensity data from digitized image |
| US7754474B2 (en) | 2005-07-05 | 2010-07-13 | 3M Innovative Properties Company | Sample processing device compression systems and methods |
| US20070009382A1 (en) | 2005-07-05 | 2007-01-11 | William Bedingham | Heating element for a rotating multiplex fluorescence detection device |
| US7527763B2 (en) | 2005-07-05 | 2009-05-05 | 3M Innovative Properties Company | Valve control system for a rotating multiplex fluorescence detection device |
| US7630849B2 (en) | 2005-09-01 | 2009-12-08 | Applied Biosystems, Llc | Method of automated calibration and diagnosis of laboratory instruments |
| US20070098594A1 (en) | 2005-11-03 | 2007-05-03 | Roche Molecular Systems, Inc. | Analytical multi-spectral optical detection system |
| US7680868B2 (en) | 2005-12-20 | 2010-03-16 | Roche Molecular Systems, Inc. | PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization |
| WO2008002765A2 (en) | 2006-06-27 | 2008-01-03 | Applera Corporation | Method and system for compensating for spatial cross-talk |
| US7839507B2 (en) * | 2006-06-28 | 2010-11-23 | Applied Biosystems, Llc | Minimizing effects of dye crosstalk |
| US20080154512A1 (en) * | 2006-07-01 | 2008-06-26 | Harrison Leong | Systems and methods for baselining and real-time pcr data analysis |
| US8005628B2 (en) | 2007-01-29 | 2011-08-23 | Applied Biosystems, Llc | Systems and methods for baseline correction using non-linear normalization |
| WO2009003645A1 (en) * | 2007-06-29 | 2009-01-08 | Roche Diagnostics Gmbh | Systems and methods for determining cross-talk coefficients in pcr and other data sets |
| US8339333B2 (en) * | 2008-01-02 | 2012-12-25 | 3M Innovative Properties Company | Methods of reducing perceived image crosstalk in a multiview display |
| AU2009240461A1 (en) | 2008-04-24 | 2009-10-29 | 3M Innovative Properties Company | Analysis of nucleic acid amplification curves using wavelet transformation |
| DK2475788T3 (en) * | 2009-09-10 | 2018-07-30 | Diasorin S P A | COMPENSATION FOR SPECTRAL CRYSTAL SAMPLES IN MULTIPLEX NUCLEIC ACID AMPLIFICATION |
-
2010
- 2010-08-31 DK DK10815913.8T patent/DK2475788T3/en active
- 2010-08-31 US US13/394,944 patent/US9938569B2/en active Active
- 2010-08-31 MX MX2012002902A patent/MX352971B/es active IP Right Grant
- 2010-08-31 PT PT181690405T patent/PT3375891T/pt unknown
- 2010-08-31 DK DK18169040.5T patent/DK3375891T3/da active
- 2010-08-31 DK DK18169032.2T patent/DK3375890T3/da active
- 2010-08-31 PT PT181690322T patent/PT3375890T/pt unknown
- 2010-08-31 EP EP18169040.5A patent/EP3375891B1/en active Active
- 2010-08-31 ES ES18169040T patent/ES2791369T3/es active Active
- 2010-08-31 ES ES10815913.8T patent/ES2680495T3/es active Active
- 2010-08-31 PT PT108159138T patent/PT2475788T/pt unknown
- 2010-08-31 EP EP18169032.2A patent/EP3375890B1/en active Active
- 2010-08-31 EP EP10815913.8A patent/EP2475788B1/en active Active
- 2010-08-31 ES ES18169032T patent/ES2785672T3/es active Active
- 2010-08-31 CN CN201080040425.5A patent/CN102482720B/zh active Active
- 2010-08-31 WO PCT/US2010/047265 patent/WO2011031585A1/en not_active Ceased
-
2018
- 2018-03-12 US US15/918,369 patent/US11603559B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20120171677A1 (en) | 2012-07-05 |
| US9938569B2 (en) | 2018-04-10 |
| DK2475788T3 (en) | 2018-07-30 |
| PT3375891T (pt) | 2020-05-11 |
| PT2475788T (pt) | 2018-07-24 |
| ES2680495T3 (es) | 2018-09-07 |
| DK3375891T3 (da) | 2020-04-27 |
| ES2791369T3 (es) | 2020-11-04 |
| MX352971B (es) | 2017-12-15 |
| ES2785672T3 (es) | 2020-10-07 |
| EP3375891B1 (en) | 2020-02-19 |
| CN102482720B (zh) | 2014-10-08 |
| PT3375890T (pt) | 2020-04-23 |
| EP3375890B1 (en) | 2020-01-22 |
| DK3375890T3 (da) | 2020-04-14 |
| US11603559B2 (en) | 2023-03-14 |
| EP3375891A1 (en) | 2018-09-19 |
| US20180201987A1 (en) | 2018-07-19 |
| WO2011031585A1 (en) | 2011-03-17 |
| EP3375890A1 (en) | 2018-09-19 |
| CN102482720A (zh) | 2012-05-30 |
| EP2475788A1 (en) | 2012-07-18 |
| EP2475788B1 (en) | 2018-04-25 |
| EP2475788A4 (en) | 2013-05-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GB | Transfer or rights |
Owner name: DIASORIN S.P.A. |
|
| FG | Grant or registration |