MX2013012709A - Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas. - Google Patents

Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas.

Info

Publication number
MX2013012709A
MX2013012709A MX2013012709A MX2013012709A MX2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A
Authority
MX
Mexico
Prior art keywords
nucleic acid
encoding nucleic
acid molecule
pesticide
encoding
Prior art date
Application number
MX2013012709A
Other languages
English (en)
Inventor
Andre R Abad
Deirdre M Kapka-Kitzman
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2013012709A publication Critical patent/MX2013012709A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporciona métodos para aislar e identificar nuevas moléculas de ácidos nucleicos codificantes de pesticidas a partir de las cepas bacterianas que se identificó que tienen un polipéptido pesticida. Los métodos incluyen seleccionar una cepa bacteriana que tiene o que se sospecha que tiene por lo menos un plásmido codificante de pesticidas, curar el por lo menos único plásmido codificante de pesticidas de la cepa bacteriana y realizar una hibridación sustractiva para obtener ARNm plasmídico que codifica el polipéptido pesticida. El ARNm plasmídico puede usarse para producir una genoteca de ADNc de la cual se puede aislar y posteriormente identificar el polipéptido pesticida.
MX2013012709A 2011-05-02 2012-04-30 Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas. MX2013012709A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161481442P 2011-05-02 2011-05-02
PCT/US2012/035740 WO2012151145A1 (en) 2011-05-02 2012-04-30 Bacterial mrna screen strategy for novel pesticide-encoding nucleic acid molecule discovery

Publications (1)

Publication Number Publication Date
MX2013012709A true MX2013012709A (es) 2013-12-06

Family

ID=46051949

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013012709A MX2013012709A (es) 2011-05-02 2012-04-30 Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas.

Country Status (7)

Country Link
US (1) US20120283111A1 (es)
EP (1) EP2705153A1 (es)
CN (1) CN103502457A (es)
BR (1) BR112013028318A2 (es)
CA (1) CA2834364A1 (es)
MX (1) MX2013012709A (es)
WO (1) WO2012151145A1 (es)

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8526774D0 (en) 1985-10-30 1985-12-04 Sandoz Ltd Bacillus thuringiensis hybrids
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5407800A (en) 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5080897A (en) * 1987-05-08 1992-01-14 Ecogen Inc. Novel bacillus thuringiensis strains, and related insecticidal compositions
GB8910624D0 (en) 1989-05-09 1989-06-21 Ici Plc Bacterial strains
US5268289A (en) 1991-12-27 1993-12-07 Epicentre Technologies Corp. Thermostable ribonuclease H
US6268181B1 (en) 1992-11-05 2001-07-31 Valent Biosciences, Corporation Methods for producing a potentiator of Bacillus pesticidal activity
US5436142A (en) 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
US5351643A (en) 1992-12-11 1994-10-04 Boyce Thompson Institute For Plant Research, Inc. High density rearing system for larvae
JP2879303B2 (ja) 1993-01-14 1999-04-05 佑 本庶 ｃＤＮＡライブラリーの作製方法、および新規なポリペプチドとそれをコードするＤＮＡ
US5849870A (en) 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
US5591575A (en) 1993-04-07 1997-01-07 Amersham International Plc Subtraction hybridization employing aziridinylbenoquinone cross-linking agents
US5512468A (en) 1993-11-12 1996-04-30 Stratagene Process of producing highly transformable bacterial cells and cells produced thereby
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5935788A (en) 1996-06-25 1999-08-10 Lifespan Biosciences, Inc. Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid
US6570005B1 (en) 1996-07-01 2003-05-27 Mycogen Corporation Toxins active against pests
US6030814A (en) 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
US6057491A (en) 1997-05-29 2000-05-02 Borad Of Regents For University Of Oklahoma Protein having insecticidal activities and method of use
AU7974198A (en) 1997-06-20 1999-01-04 Mycogen Corporation Method to identify pesticidal microbes
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation

Also Published As

Publication number Publication date
US20120283111A1 (en) 2012-11-08
BR112013028318A2 (pt) 2017-01-10
CA2834364A1 (en) 2012-11-08
EP2705153A1 (en) 2014-03-12
WO2012151145A1 (en) 2012-11-08
CN103502457A (zh) 2014-01-08

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