MX2014011165A - Cabazal integrado de lectura optoelectrónica y cartucho fluído útil para secuenciación de ácidos nucleicos. - Google Patents

Cabazal integrado de lectura optoelectrónica y cartucho fluído útil para secuenciación de ácidos nucleicos.

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Publication number
MX2014011165A
MX2014011165A MX2014011165A MX2014011165A MX2014011165A MX 2014011165 A MX2014011165 A MX 2014011165A MX 2014011165 A MX2014011165 A MX 2014011165A MX 2014011165 A MX2014011165 A MX 2014011165A MX 2014011165 A MX2014011165 A MX 2014011165A
Authority
MX
Mexico
Prior art keywords
flow cell
reading head
cartridge
nucleic acid
acid sequencing
Prior art date
Application number
MX2014011165A
Other languages
English (en)
Other versions
MX337140B (es
Inventor
Dale Buermann
John A Moon
Bryan Crane
Mark Wang
Stanley S Hong
Jason Harris
Matthew Hage
Mark J Nibbe
Original Assignee
Illumina Inc
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Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2014011165A publication Critical patent/MX2014011165A/es
Publication of MX337140B publication Critical patent/MX337140B/es

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    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52Containers specially adapted for storing or dispensing a reagent
    • B01L3/527Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • BPERFORMING OPERATIONS; TRANSPORTING
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
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    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
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    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • GPHYSICS
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
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    • G01MEASURING; TESTING
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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Abstract

Un aparato de detección que comprende un cabezal de lectura que incluye una pluralidad de microfluorómetros colocados para adquirir de manera simultánea una pluralidad de imágenes de campo amplio en un plano común; y (b) una plataforma de traslado configurada para mover el cabezal de lectura a lo largo de un sustrato que está en el plano común. El sustrato puede ser una celda de flujo que está incluida en un cartucho, el cartucho también puede incluir un alojamiento para (i) un depósito de muestras; (ii) una línea fluídica entre el depósito de muestras y la celda de flujo; (iii) varios depósitos de reactivos en comunicación de fluidos con la celda de flujo, (iv) al menos una válvula configurada para mediar la comunicación de fluidos entre los depósitos y la celda de flujo; (v) al menos una fuente de presión configurada para mover los líquidos de los depósitos a la celda de flujo. El aparato de detección y el cartucho se pueden utilizar juntos o independientes uno del otro.
MX2014011165A 2012-04-03 2013-02-13 Cabazal integrado de lectura optoelectrónica y cartucho fluído útil para secuenciación de ácidos nucleicos. MX337140B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261619784P 2012-04-03 2012-04-03
PCT/US2013/025963 WO2013151622A1 (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Publications (2)

Publication Number Publication Date
MX2014011165A true MX2014011165A (es) 2015-03-19
MX337140B MX337140B (es) 2016-02-12

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MX2014011165A MX337140B (es) 2012-04-03 2013-02-13 Cabazal integrado de lectura optoelectrónica y cartucho fluído útil para secuenciación de ácidos nucleicos.

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US (5) US9193996B2 (es)
EP (2) EP4219012A1 (es)
JP (1) JP6159391B2 (es)
KR (3) KR102118211B1 (es)
CN (1) CN204832037U (es)
AU (4) AU2013243998B2 (es)
BR (2) BR122021002358B8 (es)
CA (2) CA2867665C (es)
ES (1) ES2949570T3 (es)
IL (3) IL234864B (es)
IN (1) IN2014DN07992A (es)
MX (1) MX337140B (es)
WO (1) WO2013151622A1 (es)

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AU2016222420A1 (en) 2016-09-22
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AU2019200400A1 (en) 2019-02-07
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AU2013243998A1 (en) 2014-10-09
BR112014024789B1 (pt) 2021-05-25

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