MX2016010285A - Ingenieria genetica multiplexada habilitada por crispr. - Google Patents

Ingenieria genetica multiplexada habilitada por crispr.

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Publication number
MX2016010285A
MX2016010285A MX2016010285A MX2016010285A MX2016010285A MX 2016010285 A MX2016010285 A MX 2016010285A MX 2016010285 A MX2016010285 A MX 2016010285A MX 2016010285 A MX2016010285 A MX 2016010285A MX 2016010285 A MX2016010285 A MX 2016010285A
Authority
MX
Mexico
Prior art keywords
crispr
genetic engineering
multiplexed genetic
enabled
multiplexed
Prior art date
Application number
MX2016010285A
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English (en)
Other versions
MX375420B (es
Inventor
Ryan T Gill
Garst Andrew
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Univ Colorado Regents
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53800598&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016010285(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Colorado Regents filed Critical Univ Colorado Regents
Publication of MX2016010285A publication Critical patent/MX2016010285A/es
Publication of MX375420B publication Critical patent/MX375420B/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B40/04—Libraries containing only organic compounds
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/50—Physical structure
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Se describen aquí métodos y vectores para la manipulación multiplexada, racional de cromosomas dentro de marcos de lectura abierta (por ejemplo, en bibliotecas de proteína) o cualquier segmento de un cromosoma en una célula o población de células, en el cual son usadas variaciones de sistemas CRISPR.
MX2016010285A 2014-02-11 2015-02-11 Ingenieria genetica multiplexada habilitada por crispr. MX375420B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461938608P 2014-02-11 2014-02-11
PCT/US2015/015476 WO2015123339A1 (en) 2014-02-11 2015-02-11 Crispr enabled multiplexed genome engineering

Publications (2)

Publication Number Publication Date
MX2016010285A true MX2016010285A (es) 2017-01-11
MX375420B MX375420B (es) 2025-03-06

Family

ID=53800598

Family Applications (1)

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MX2016010285A MX375420B (es) 2014-02-11 2015-02-11 Ingenieria genetica multiplexada habilitada por crispr.

Country Status (23)

Country Link
US (16) US10266849B2 (es)
EP (4) EP4063503A1 (es)
JP (3) JP2017505145A (es)
KR (4) KR102496984B1 (es)
CN (2) CN111705365B (es)
AU (2) AU2015217208B2 (es)
CA (3) CA3075047C (es)
CY (1) CY1123115T1 (es)
DE (1) DE212015000061U1 (es)
DK (1) DK3105328T3 (es)
ES (2) ES2974229T3 (es)
GB (1) GB2544382B (es)
HR (1) HRP20201040T1 (es)
HU (2) HUE066611T2 (es)
IL (2) IL246951B (es)
LT (1) LT3105328T (es)
MX (1) MX375420B (es)
PL (1) PL3105328T3 (es)
PT (1) PT3105328T (es)
RS (1) RS60492B1 (es)
SI (1) SI3105328T1 (es)
SM (1) SMT202000357T1 (es)
WO (1) WO2015123339A1 (es)

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