MX2017005306A - Novedosos sitios de integracion en celulas cho y usos de estos. - Google Patents

Novedosos sitios de integracion en celulas cho y usos de estos.

Info

Publication number
MX2017005306A
MX2017005306A MX2017005306A MX2017005306A MX2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A
Authority
MX
Mexico
Prior art keywords
expression
integration
cho cells
new sites
eukaryotic
Prior art date
Application number
MX2017005306A
Other languages
English (en)
Inventor
Chen Gang
Burakov Darya
Shen Ying
P Fandl James
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2017005306(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2017005306A publication Critical patent/MX2017005306A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Se proporcionan secuencias de nucleótidos potenciadoras de la expresión para sistemas de expresión en eucariotas que permiten la expresión estable y aumentada de proteínas recombinantes en células eucariotas. Se proporcionan sitios de integración genómica que proporcionan expresión aumentada y métodos de uso de estos para la expresión de un gen de interés en una célula eucariota. Se proporcionan loci cromosómicos, secuencia, y vectores para la expresión estable y aumentada de genes en células eucariotas.
MX2017005306A 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos. MX2017005306A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (1)

Publication Number Publication Date
MX2017005306A true MX2017005306A (es) 2018-01-09

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017005306A MX2017005306A (es) 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos.

Country Status (16)

Country Link
US (5) US9816110B2 (es)
EP (1) EP3209785B1 (es)
JP (1) JP6668340B2 (es)
KR (1) KR102243243B1 (es)
CN (1) CN107109434A (es)
AR (1) AR102420A1 (es)
AU (1) AU2015335921B2 (es)
BR (1) BR112017008022A2 (es)
CA (1) CA2965495C (es)
EA (1) EA037255B1 (es)
IL (1) IL251674B (es)
MX (1) MX2017005306A (es)
MY (1) MY184103A (es)
SG (1) SG11201703149RA (es)
TW (2) TWI780847B (es)
WO (1) WO2016064999A1 (es)

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WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
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CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
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CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
AU2019403279A1 (en) * 2018-12-21 2021-07-01 Genentech, Inc. Targeted integration of nucleic acids
KR102655024B1 (ko) 2019-04-02 2024-04-04 추가이 세이야쿠 가부시키가이샤 표적 특이적인 외래 유전자의 도입 방법
WO2020264253A1 (en) 2019-06-26 2020-12-30 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
KR20230019156A (ko) * 2020-06-02 2023-02-07 카탈렌트 파마 솔루션즈, 엘엘씨 유전자 삽입을 위한 다수의 도크들이 있는 세포주
WO2021262798A1 (en) * 2020-06-24 2021-12-30 Genentech, Inc. Targeted integration of nucleic acids
EP4229205B1 (en) 2020-10-15 2025-05-14 F. Hoffmann-La Roche AG Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CA3235566A1 (en) 2021-10-18 2023-04-27 Michael Goren Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
MX2024004691A (es) 2021-10-18 2024-05-23 Regeneron Pharma Transcripcion controlada de polinucleotidos.
AU2022373653A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
KR20240139051A (ko) * 2022-01-28 2024-09-20 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 이종이식을 위한 유전 변형
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
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CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
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Also Published As

Publication number Publication date
BR112017008022A2 (pt) 2018-02-20
JP6668340B2 (ja) 2020-03-18
KR20170096999A (ko) 2017-08-25
CA2965495C (en) 2022-05-24
US20240018553A1 (en) 2024-01-18
JP2017535258A (ja) 2017-11-30
TWI780847B (zh) 2022-10-11
SG11201703149RA (en) 2018-02-27
WO2016064999A1 (en) 2016-04-28
CA2965495A1 (en) 2016-04-28
US20200002732A1 (en) 2020-01-02
US12565667B2 (en) 2026-03-03
AR102420A1 (es) 2017-03-01
TW202144576A (zh) 2021-12-01
EA037255B1 (ru) 2021-02-26
EP3209785A1 (en) 2017-08-30
TWI747808B (zh) 2021-12-01
US11268109B2 (en) 2022-03-08
EP3209785B1 (en) 2019-07-31
AU2015335921B2 (en) 2020-06-25
US20210171984A1 (en) 2021-06-10
EA201790698A1 (ru) 2018-01-31
MY184103A (en) 2021-03-18
TW201629229A (zh) 2016-08-16
US20180030480A1 (en) 2018-02-01
US9816110B2 (en) 2017-11-14
US20160115502A1 (en) 2016-04-28
IL251674A0 (en) 2017-06-29
US11788102B2 (en) 2023-10-17
AU2015335921A1 (en) 2017-05-04
IL251674B (en) 2020-10-29
CN107109434A (zh) 2017-08-29
KR102243243B1 (ko) 2021-04-22

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