MX2017013022A - Metodo para bloquear miarn. - Google Patents

Metodo para bloquear miarn.

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Publication number
MX2017013022A
MX2017013022A MX2017013022A MX2017013022A MX2017013022A MX 2017013022 A MX2017013022 A MX 2017013022A MX 2017013022 A MX2017013022 A MX 2017013022A MX 2017013022 A MX2017013022 A MX 2017013022A MX 2017013022 A MX2017013022 A MX 2017013022A
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MX
Mexico
Prior art keywords
blocking
nucleic acid
region
mirna
miarn
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MX2017013022A
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English (en)
Other versions
MX386064B (es
Inventor
M Myers Richard
s roberts Brian
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Hudsonalpha Inst For Biotechnology
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Application filed by Hudsonalpha Inst For Biotechnology filed Critical Hudsonalpha Inst For Biotechnology
Publication of MX2017013022A publication Critical patent/MX2017013022A/es
Publication of MX386064B publication Critical patent/MX386064B/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12N2330/00—Production
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/207—Modifications characterised by siRNA, miRNA
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Se proporciona un &do nucleico de bloqueo para usarse en la reducción de la abundancia de un micro-ARN no objetivo (miARN) en una biblioteca de miARN, incluyendo: una región complementaria de cadena sencilla en uno de los extremos 5' del Ácido nucleico de bloqueo 0 el extremo 3' del ácido nucleico de bloqueo, que se recose con una región de unión en un primer extremo del miARN no deseado; una región de formación de tallo-bucle u otra región de cadena doble adyacente a la región complementaria, en la cual todos los extremos terminales del ácido nucleico de bloqueo excepto uno no están disponibles para participar en reacciones de ligasa; también se proporcionan métodos y kits para utilizar el ácido nucleico de bloqueo.
MX2017013022A 2015-04-10 2016-04-10 Metodo para bloquear miarn. MX386064B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562146015P 2015-04-10 2015-04-10
PCT/US2016/026846 WO2016164866A1 (en) 2015-04-10 2016-04-10 METHOD FOR BLOCKING miRNA

Publications (2)

Publication Number Publication Date
MX2017013022A true MX2017013022A (es) 2018-03-15
MX386064B MX386064B (es) 2025-03-18

Family

ID=57072747

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017013022A MX386064B (es) 2015-04-10 2016-04-10 Metodo para bloquear miarn.

Country Status (8)

Country Link
US (2) US10480023B2 (es)
EP (1) EP3280805B1 (es)
JP (1) JP6791875B2 (es)
AU (1) AU2016246820B2 (es)
CA (1) CA2982369A1 (es)
IL (1) IL254972B (es)
MX (1) MX386064B (es)
WO (1) WO2016164866A1 (es)

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US20160319278A1 (en) 2015-04-03 2016-11-03 University Of Massachusetts Fully stabilized asymmetric sirna
US11396650B2 (en) 2015-06-02 2022-07-26 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches
WO2017030973A1 (en) 2015-08-14 2017-02-23 University Of Massachusetts Bioactive conjugates for oligonucleotide delivery
US10478503B2 (en) 2016-01-31 2019-11-19 University Of Massachusetts Branched oligonucleotides
US10287625B2 (en) * 2016-03-18 2019-05-14 Norgen Biotek Corp. Methods and kits for separating nucleic acids by size
US12123869B2 (en) 2016-03-23 2024-10-22 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
CA3033368A1 (en) 2016-08-12 2018-02-15 University Of Massachusetts Conjugated oligonucleotides
US12252507B2 (en) 2016-12-05 2025-03-18 Children's Medical Center Corporation Nucleic acid nanoswitch construction methods
WO2018157100A1 (en) * 2017-02-27 2018-08-30 Bioo Scientific Corporation Methods and kits for depleting undesired nucleic acids
JP7406793B2 (ja) 2017-06-23 2023-12-28 ユニバーシティー オブ マサチューセッツ ２テイル自己デリバリー型ｓｉＲＮＡおよび関連方法
EP3681895A4 (en) * 2017-09-13 2021-05-26 Becton, Dickinson and Company METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES
US11591636B2 (en) 2017-11-20 2023-02-28 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
CN108676846B (zh) * 2018-05-25 2022-04-01 艾吉泰康生物科技(北京)有限公司 一种桥式寡核苷酸在文库目标区域捕获中的应用
WO2020033899A1 (en) 2018-08-10 2020-02-13 University Of Massachusetts Modified oligonucleotides targeting snps
US11279930B2 (en) 2018-08-23 2022-03-22 University Of Massachusetts O-methyl rich fully stabilized oligonucleotides
CN113614232A (zh) * 2019-01-18 2021-11-05 马萨诸塞大学 动态药代动力学修饰锚
KR20220047989A (ko) 2019-08-09 2022-04-19 유니버시티 오브 매사추세츠 Snp를 표적화하는 화학적으로 변형된 올리고뉴클레오타이드
US12365894B2 (en) 2019-09-16 2025-07-22 University Of Massachusetts Branched lipid conjugates of siRNA for specific tissue delivery
WO2021174071A1 (en) 2020-02-29 2021-09-02 President And Fellows Of Harvard College Nanoswitch caliper trains for high-throughput, high-resolution structural analysis of biomolecules
WO2021242883A1 (en) 2020-05-26 2021-12-02 University Of Massachusetts Synthetic oligonucleotides having regions of block and cluster modifications
CN117677699A (zh) 2021-06-23 2024-03-08 马萨诸塞大学 用于治疗先兆子痫和其他血管生成病症的优化抗flt1寡核苷酸化合物
CN118139978A (zh) * 2021-10-22 2024-06-04 蜂鸟诊断有限责任公司 使用反义寡核苷酸靶向抑制逆转录
US20230323341A1 (en) * 2022-03-25 2023-10-12 Bioo Scientific Corporation Methods, compositions, and kits for inhibiting formation of adapter dimers

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US20030211483A1 (en) * 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
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Also Published As

Publication number Publication date
EP3280805A1 (en) 2018-02-14
CA2982369A1 (en) 2016-10-13
EP3280805B1 (en) 2020-12-16
IL254972A0 (en) 2017-12-31
IL254972B (en) 2021-05-31
BR112017021854A2 (pt) 2018-07-10
EP3280805A4 (en) 2019-03-13
MX386064B (es) 2025-03-18
US10480023B2 (en) 2019-11-19
JP6791875B2 (ja) 2020-11-25
US20180073066A1 (en) 2018-03-15
US20200024652A1 (en) 2020-01-23
JP2018513685A (ja) 2018-05-31
US11781176B2 (en) 2023-10-10
AU2016246820B2 (en) 2021-08-12
WO2016164866A1 (en) 2016-10-13
AU2016246820A1 (en) 2017-11-30

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