MX2020006321A - Celulas estromales mesenquimales y metodos para obtener celulas estromales mesenquimales del cordon umbilical. - Google Patents

Celulas estromales mesenquimales y metodos para obtener celulas estromales mesenquimales del cordon umbilical.

Info

Publication number
MX2020006321A
MX2020006321A MX2020006321A MX2020006321A MX2020006321A MX 2020006321 A MX2020006321 A MX 2020006321A MX 2020006321 A MX2020006321 A MX 2020006321A MX 2020006321 A MX2020006321 A MX 2020006321A MX 2020006321 A MX2020006321 A MX 2020006321A
Authority
MX
Mexico
Prior art keywords
stromal cells
mesenchymal stromal
umbilical cord
stromal
cells
Prior art date
Application number
MX2020006321A
Other languages
English (en)
Inventor
Diego Ardigo''
Giovanni Milazzo
Alba Murgia
Massimo Dominici
Original Assignee
Chiesi Farm Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiesi Farm Spa filed Critical Chiesi Farm Spa
Publication of MX2020006321A publication Critical patent/MX2020006321A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a células estromales mesenquimales y a un método ventajoso para su aislamiento del cordón umbilical. En particular, las células se pueden obtener por medio de un método que comprende aislar la Jalea de Wharton estromal o una fracción de la misma de un cordón umbilical y sujetar la Jalea de Wharton estromal o una fracción de la misma a un tratamiento enzimático, para liberar en consecuencia por lo menos una célula de la Jalea de Wharton estromal o una fracción de la misma. Preferiblemente, el cultivo de células ocurre bajo condiciones que permiten la expansión de la población de células. La población de células de acuerdo con la presente invención tiene propiedades proliferativas y es de interés industrial para una variedad de propósitos.
MX2020006321A 2017-12-22 2018-12-21 Celulas estromales mesenquimales y metodos para obtener celulas estromales mesenquimales del cordon umbilical. MX2020006321A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IB2017058366 2017-12-22
PCT/IB2018/060503 WO2019123407A1 (en) 2017-12-22 2018-12-21 Mesenchymal stromal cells and methods for obtaining mesenchymal stromal cells from umbilical cord

Publications (1)

Publication Number Publication Date
MX2020006321A true MX2020006321A (es) 2020-09-18

Family

ID=61022383

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020006321A MX2020006321A (es) 2017-12-22 2018-12-21 Celulas estromales mesenquimales y metodos para obtener celulas estromales mesenquimales del cordon umbilical.

Country Status (11)

Country Link
US (1) US20200339947A1 (es)
EP (1) EP3728566B1 (es)
JP (1) JP7286651B2 (es)
KR (1) KR102777940B1 (es)
CN (1) CN111492051B (es)
AU (1) AU2018393063B2 (es)
BR (1) BR112020012436A2 (es)
CA (1) CA3081647A1 (es)
ES (1) ES2985827T3 (es)
MX (1) MX2020006321A (es)
WO (1) WO2019123407A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111117954A (zh) * 2020-01-18 2020-05-08 北京永华燕芸生物科技有限公司 一种脐带来源原代间充质干细胞的分离培养方法
RU2744301C1 (ru) * 2020-07-21 2021-03-05 федеральное государственное автономное образовательное учреждение высшего образования Первый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский университет) (ФГАОУ ВО Первый МГМУ им. И.М. Сеченова Минздрава России (Се Способ получения мультипотентных мезенхимных стромальных клеток из пупочного канатика новорожденного
US20220296645A1 (en) * 2021-03-22 2022-09-22 Spiritus Therapeutics, Inc. Diagnostic and therapeutic uses of compositions comprising purified, enriched potent exosomes containing disease-based and therapy based signature cargo
CN116926001A (zh) * 2022-04-06 2023-10-24 上海我武干细胞科技有限公司 血清替代物及其制备方法与应用
CN115025288B (zh) * 2022-06-17 2023-06-13 中南大学湘雅医院 一种外泌体水凝胶混合体系及其制备方法
EP4744672A1 (en) 2024-11-13 2026-05-20 UMC Utrecht Holding B.V. Intranasal application of mesenchymal stem cells in the treatment of neonatal hypoxic-ischemic brain injuries

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5919702A (en) 1996-10-23 1999-07-06 Advanced Tissue Science, Inc. Production of cartilage tissue using cells isolated from Wharton's jelly
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
JP4790592B2 (ja) * 2003-02-11 2011-10-12 ダビース，ジヨン・イー ヒト臍帯のウォートンジェリーからの前駆細胞
CA2564679C (en) 2004-03-22 2015-06-23 Osiris Therapeutics, Inc. Mesenchymal stem cells and uses therefor
US8241902B2 (en) 2006-01-11 2012-08-14 Technion Research & Development Foundation Ltd. Preparation of adult stem cell-derived connective tissue progenitors
US8071135B2 (en) 2006-10-04 2011-12-06 Anthrogenesis Corporation Placental tissue compositions
KR100900309B1 (ko) 2007-05-29 2009-06-02 차의과학대학교 산학협력단 태반 융모막판막-유래 중간엽 줄기 세포의 고순도 분리방법
EP2513300A1 (en) * 2009-12-18 2012-10-24 C.B.B. Lifeline Biotech Ltd Methods for isolating mononuclear cells that include a subpopulation of mesenchymal progenitor cells and vascular cells that include a subpopulation of endothelial progenitor cells from umbilical cord tissue
CN101974484B (zh) * 2010-11-03 2013-01-30 江苏省北科生物科技有限公司 一种人脐带间充质干细胞的制备方法
WO2013003356A1 (en) 2011-06-27 2013-01-03 Emory University Compositions, uses, and preparation of platelet lysates
ITRM20110500A1 (it) 2011-09-23 2013-03-24 Futura Stem Cells Sa Lisato piastrinico, usi di esso e metodo per la sua preparazione
US9315776B2 (en) * 2011-11-09 2016-04-19 National University Of Singapore Wharton's jelly mesenchymal stem cells and uses thereof
SG11201806561UA (en) 2015-10-02 2018-09-27 Elixir Biopharma Sdn Bhd Method of isolating mesenchymal stromal cells and applications for tissue engineering
CN106434546B (zh) * 2016-12-17 2024-07-23 重庆市干细胞技术有限公司 完全利用脐带资源制备间充质干细胞的方法

Also Published As

Publication number Publication date
WO2019123407A1 (en) 2019-06-27
BR112020012436A2 (pt) 2020-11-24
JP2021507711A (ja) 2021-02-25
ES2985827T3 (es) 2024-11-07
RU2020123383A3 (es) 2022-04-20
RU2020123383A (ru) 2022-01-24
CA3081647A1 (en) 2019-06-27
EP3728566C0 (en) 2024-08-21
AU2018393063A1 (en) 2020-06-18
CN111492051B (zh) 2024-02-23
CN111492051A (zh) 2020-08-04
JP7286651B2 (ja) 2023-06-05
AU2018393063B2 (en) 2025-02-06
US20200339947A1 (en) 2020-10-29
EP3728566A1 (en) 2020-10-28
KR20200104300A (ko) 2020-09-03
KR102777940B1 (ko) 2025-03-11
EP3728566B1 (en) 2024-08-21

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