MX2020009556A - Metodo para producir molecula de acido ribonucleico (arn) de hebra unica en horquilla. - Google Patents

Metodo para producir molecula de acido ribonucleico (arn) de hebra unica en horquilla.

Info

Publication number
MX2020009556A
MX2020009556A MX2020009556A MX2020009556A MX2020009556A MX 2020009556 A MX2020009556 A MX 2020009556A MX 2020009556 A MX2020009556 A MX 2020009556A MX 2020009556 A MX2020009556 A MX 2020009556A MX 2020009556 A MX2020009556 A MX 2020009556A
Authority
MX
Mexico
Prior art keywords
stranded
molecule
stranded oligorna
rna molecule
stranded rna
Prior art date
Application number
MX2020009556A
Other languages
English (en)
Inventor
Katsuhiko Iseki
Keiichi Okimura
Hideaki Inada
Masato Sanosaka
Ayumi TAKASHINA
Original Assignee
Toray Industries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries filed Critical Toray Industries
Publication of MX2020009556A publication Critical patent/MX2020009556A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01003—RNA ligase (ATP) (6.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere a un método para producir una molécula de ARN de hebra única en horquilla capaz de inhibir la expresión de un gen objetivo, el método comprende: (i) una etapa de hibridación para hibridar una primera molécula de oligoARN de hebra única y una segunda molécula de oligoARN de hebra única; y (ii) una etapa de ligación para ligar el extremo 3´ de la primera molécula de oligoARN de hebra única y el extremo 5´ de la segunda molécula de oligoARN de hebra única por una ligasa de la familia Rnl2, en donde una secuencia producida por ligación de la primera molécula de oligoARN de hebra única y la segunda molécula de oligoARN de hebra única comprende una secuencia que inhibe la expresión del gen el gen objetivo.
MX2020009556A 2018-03-30 2019-03-29 Metodo para producir molecula de acido ribonucleico (arn) de hebra unica en horquilla. MX2020009556A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018070423 2018-03-30
PCT/JP2019/013923 WO2019189722A1 (ja) 2018-03-30 2019-03-29 ヘアピン型一本鎖ｒｎａ分子の製造方法

Publications (1)

Publication Number Publication Date
MX2020009556A true MX2020009556A (es) 2020-10-05

Family

ID=68061953

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020009556A MX2020009556A (es) 2018-03-30 2019-03-29 Metodo para producir molecula de acido ribonucleico (arn) de hebra unica en horquilla.

Country Status (16)

Country Link
US (2) US11920131B2 (es)
EP (1) EP3778886A4 (es)
JP (2) JP6631751B1 (es)
KR (1) KR20200136363A (es)
CN (1) CN111819280A (es)
AU (1) AU2019242331A1 (es)
BR (1) BR112020017769A2 (es)
CA (1) CA3094160A1 (es)
IL (1) IL277346A (es)
MX (1) MX2020009556A (es)
PH (1) PH12020551487A1 (es)
RU (1) RU2020130258A (es)
SA (1) SA520420072B1 (es)
TW (1) TWI772632B (es)
WO (1) WO2019189722A1 (es)
ZA (1) ZA202005149B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI848981B (zh) 2018-10-02 2024-07-21 日商東麗股份有限公司 新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法
EP4069838A1 (en) * 2019-12-03 2022-10-12 Beam Therapeutics Inc. Synthetic guide rna, compositions, methods, and uses thereof
JP7613130B2 (ja) * 2020-03-25 2025-01-15 味の素株式会社 リガーゼ変異体
US12522629B2 (en) 2020-03-27 2026-01-13 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
KR20240046780A (ko) * 2021-08-27 2024-04-09 페킹 유니버시티 원형 rna를 제조하기 위한 구축물 및 방법
CN117660374A (zh) * 2022-09-08 2024-03-08 凯莱英医药集团（天津）股份有限公司 Rna连接酶在寡核苷酸制备上的应用
CN115774075B (zh) * 2023-02-15 2023-06-06 江苏耀海生物制药有限公司 一种基于RP-HPLC分析体外转录产物成分circRNA的方法

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WO2001040516A2 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
AU2003256857A1 (en) * 2002-08-08 2004-02-25 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
JP5296328B2 (ja) * 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
EP2256191A4 (en) * 2008-02-15 2011-07-06 Riken CYCLIC SINGLE NUCLEIC ACID COMPLEX AND METHOD FOR THE PRODUCTION THEREOF
JPWO2011052013A1 (ja) 2009-10-29 2013-03-14 株式会社バイオダイナミクス研究所 核酸鎖の結合および修飾方法
HUE037500T2 (hu) 2010-07-08 2018-08-28 Bonac Corp Egyszálú nukleinsav molekula a génexpresszió szabályozására
ES2527660T3 (es) 2010-08-03 2015-01-28 Bonac Corporation Molécula de ARN monocatenario que tiene esqueleto alicíclico que contiene nitrógeno
WO2013027843A1 (ja) 2011-08-25 2013-02-28 株式会社ボナック 配糖体化合物、チオエーテルの製造方法、エーテル、エーテルの製造方法、配糖体化合物の製造方法、核酸の製造方法
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Also Published As

Publication number Publication date
PH12020551487A1 (en) 2021-08-23
ZA202005149B (en) 2022-01-26
EP3778886A1 (en) 2021-02-17
BR112020017769A2 (pt) 2021-01-05
RU2020130258A (ru) 2022-05-04
US11920131B2 (en) 2024-03-05
SA520420072B1 (ar) 2024-04-21
JPWO2019189722A1 (ja) 2020-04-30
US20210024930A1 (en) 2021-01-28
JP6631751B1 (ja) 2020-01-15
TW202003842A (zh) 2020-01-16
KR20200136363A (ko) 2020-12-07
AU2019242331A1 (en) 2020-09-10
EP3778886A4 (en) 2022-11-02
CN111819280A (zh) 2020-10-23
JP2019213567A (ja) 2019-12-19
TWI772632B (zh) 2022-08-01
JP7363316B2 (ja) 2023-10-18
US20240167033A1 (en) 2024-05-23
CA3094160A1 (en) 2019-10-03
WO2019189722A1 (ja) 2019-10-03
IL277346A (en) 2020-10-29

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