MX2024007773A - Métodos y sistemas para optimizar las condiciones de cultivo en un proceso de cultivo. - Google Patents

Métodos y sistemas para optimizar las condiciones de cultivo en un proceso de cultivo.

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Publication number
MX2024007773A
MX2024007773A MX2024007773A MX2024007773A MX2024007773A MX 2024007773 A MX2024007773 A MX 2024007773A MX 2024007773 A MX2024007773 A MX 2024007773A MX 2024007773 A MX2024007773 A MX 2024007773A MX 2024007773 A MX2024007773 A MX 2024007773A
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MX
Mexico
Prior art keywords
culture
cells
output
measure
new
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MX2024007773A
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English (en)
Inventor
Shannon Hall
Ouwei Wang
Brian Lee
Charles Emrich
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Pow Genetic Solutions Inc
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Publication date
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Publication of MX2024007773A publication Critical patent/MX2024007773A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Computer Hardware Design (AREA)
  • Thermal Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proporcionan métodos y sistemas para mejorar iterativamente las condiciones de cultivo para al menos una cantidad de producto generado por cultivo en un proceso de cultivo. Los métodos comprenden: a) proporcionar un cultivo continuo de células en un biorreactor, en donde las células se cultivan en condiciones de cultivo que comprenden un conjunto de parámetros de cultivo, y en donde el cultivo celular produce al menos una cantidad de producto generado por cultivo en una primera medida; b) mientras se mantienen las células en cultivo continuo, probar iterativamente diferentes condiciones de cultivo mediante: (i) seleccionar una nueva condición de cultivo que comprende un nuevo conjunto de parámetros de cultivo; en donde se predice que la nueva condición de cultivo mejorará la medida de la cantidad de producto generado por cultivo, y se selecciona basándose en una o más medidas previas de la cantidad de producto generado por cultivo; (ii) perturbar las células con las nuevas condiciones de cultivo; y (iii) determinar una nueva medida de la cantidad de producto generado por cultivo; en donde se itera la prueba para producir una pluralidad de mejoras en la medida de la cantidad de producto generado por cultivo.. El proceso iterativo puede ser realizado por un agente automatizado que utilice métodos de búsqueda directa o técnicas de aprendizaje profundo, como el aprendizaje por refuerzo, para encontrar rápidamente condiciones de cultivo mejoradas
MX2024007773A 2021-12-21 2024-06-20 Métodos y sistemas para optimizar las condiciones de cultivo en un proceso de cultivo. MX2024007773A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163292397P 2021-12-21 2021-12-21
PCT/US2022/053733 WO2023122224A2 (en) 2021-12-21 2022-12-21 Methods and systems for optimizing culture conditions in a culture process

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MX2024007773A true MX2024007773A (es) 2024-12-06

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US (1) US20240209306A1 (es)
EP (1) EP4453183A4 (es)
JP (1) JP2025503486A (es)
KR (1) KR20240134140A (es)
CN (1) CN118805224A (es)
AU (1) AU2022418603A1 (es)
CA (1) CA3241931A1 (es)
IL (1) IL313759A (es)
MX (1) MX2024007773A (es)
WO (1) WO2023122224A2 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025006354A2 (en) * 2023-06-28 2025-01-02 Pow Genetic Solutions, Inc. Methods and systems for optimizing culture conditions in a culture process
CN117253555A (zh) * 2023-11-17 2023-12-19 山东阜丰发酵有限公司 一种改进黄原胶发酵工艺的方法及其运行系统
CN117745148B (zh) * 2024-02-10 2024-05-10 安徽省农业科学院烟草研究所 基于多源数据的稻茬烤烟种植质量评价方法及系统
CN119506387A (zh) * 2024-10-14 2025-02-25 苏州启和医疗科技有限公司 一种用于转染试剂高精度测试的转染效率检测方法
CN120015152B (zh) * 2025-04-21 2025-08-12 苏州工业园区旭太生物工程有限公司 一种多肽诱导型生物发酵培养基优化方法及系统
CN120183511A (zh) * 2025-05-22 2025-06-20 成都英德生物医药设备有限公司 生物反应器的动态优化方法以及系统

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US20060199260A1 (en) * 2002-05-01 2006-09-07 Zhiyu Zhang Microbioreactor for continuous cell culture
US20070037276A1 (en) * 2004-02-23 2007-02-15 Eudes Francois Marie De Crecy Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner
US10479969B2 (en) * 2010-10-11 2019-11-19 Phycoil Biotechnology International. Inc. Utilization of wastewater for microalgal cultivation
WO2018229802A1 (en) * 2017-06-16 2018-12-20 Ge Healthcare Bio-Sciences Ab Method for predicting outcome of and modelling of a process in a bioreactor
JP7092879B2 (ja) * 2017-12-29 2022-06-28 エフ．ホフマン－ラ ロシュ アーゲー 細胞培養物の代謝状態の予測
EP3819368B1 (en) * 2019-11-08 2022-03-30 Ceidos SA Cell culture monitoring system
EP4116403A1 (en) * 2021-07-07 2023-01-11 Sartorius Stedim Data Analytics AB Monitoring, simulation and control of bioprocesses

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JP2025503486A (ja) 2025-02-04
WO2023122224A3 (en) 2023-09-14
KR20240134140A (ko) 2024-09-06
IL313759A (en) 2024-08-01
EP4453183A4 (en) 2025-12-24
CA3241931A1 (en) 2023-06-29
CN118805224A (zh) 2024-10-18
WO2023122224A2 (en) 2023-06-29
EP4453183A2 (en) 2024-10-30
US20240209306A1 (en) 2024-06-27
AU2022418603A1 (en) 2024-08-01

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