MXPA03007760A - Analisis para identificar los inhibidores de la arn-polimerasa (ns5b) del hcv, dependiente del arn. - Google Patents

Analisis para identificar los inhibidores de la arn-polimerasa (ns5b) del hcv, dependiente del arn.

Info

Publication number
MXPA03007760A
MXPA03007760A MXPA03007760A MXPA03007760A MXPA03007760A MX PA03007760 A MXPA03007760 A MX PA03007760A MX PA03007760 A MXPA03007760 A MX PA03007760A MX PA03007760 A MXPA03007760 A MX PA03007760A MX PA03007760 A MXPA03007760 A MX PA03007760A
Authority
MX
Mexico
Prior art keywords
polymerase
absence
primer
products formed
hcv ns5b
Prior art date
Application number
MXPA03007760A
Other languages
English (en)
Inventor
George Kukolj
Original Assignee
Boehringer Ingelheim Ca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Ca Ltd filed Critical Boehringer Ingelheim Ca Ltd
Publication of MXPA03007760A publication Critical patent/MXPA03007760A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D235/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, condensed with other rings
    • C07D235/02—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, condensed with other rings condensed with carbocyclic rings or ring systems
    • C07D235/04—Benzimidazoles; Hydrogenated benzimidazoles
    • C07D235/18—Benzimidazoles; Hydrogenated benzimidazoles with aryl radicals directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/04—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
    • C07D409/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invencion proporciona un novedoso metodo para la identificacion de inhibidores de la polimerasa NS5B del HCV, que utiliza una ARN-polimerasa dependiente del ARN de NS5B del HCV que tiene una Km mayor que la polimerasa NS5B nativa, de longitud completa, para asegurar la identificacion de los inhibidores del enlace cebador-plantilla de la polimerasa del HCV, incluyendo compuestos moderadamente inhibidores. El metodo incluye la provision de una polimerasa NS5B del HCV, de afinidad disminuida, un cebador-plantilla apropiado, y una pluralidad de trifosfatos de ribonucleotido apropiados, la incubacion de la polimerasa NS5B del HCV con el cebador-plantilla en presencia y ausencia de un inhibidor potencial, la medicion de la presencia de cualesquiera productos de polimerasa formados en presencia y ausencia del inhibidor potencial; y la comparacion de la cantidad de los productos de polimerasa formados en presencia y ausencia del inhibidor potencial; en donde una disminucion en la cantidad de los productos de polimerasa formados en presencia del inhibidor potencial, en comparacion a la cantidad de los productos de polimerasa formados en ausencia del inhibidor potencial, es indicadora de un inhibidor potencial del enlace cebador-plantilla de la ARN-polimerasa dependiente del ARN de NS5B del HCV.
MXPA03007760A 2001-03-08 2002-03-06 Analisis para identificar los inhibidores de la arn-polimerasa (ns5b) del hcv, dependiente del arn. MXPA03007760A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27437401P 2001-03-08 2001-03-08
PCT/CA2002/000323 WO2002070739A2 (en) 2001-03-08 2002-03-06 Assay for identifying inhibitors of the rna dependent rna polymerase (ns5b) of hcv

Publications (1)

Publication Number Publication Date
MXPA03007760A true MXPA03007760A (es) 2004-03-16

Family

ID=23047904

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA03007760A MXPA03007760A (es) 2001-03-08 2002-03-06 Analisis para identificar los inhibidores de la arn-polimerasa (ns5b) del hcv, dependiente del arn.

Country Status (9)

Country Link
US (1) US20040110126A1 (es)
EP (1) EP1370682A2 (es)
JP (1) JP2004520839A (es)
CA (1) CA2439176A1 (es)
HU (1) HUP0400039A3 (es)
IL (1) IL157280A0 (es)
MX (1) MXPA03007760A (es)
NZ (1) NZ528644A (es)
WO (1) WO2002070739A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2335700A1 (en) * 2001-07-25 2011-06-22 Boehringer Ingelheim (Canada) Ltd. Hepatitis C virus polymerase inhibitors with a heterobicylic structure
US7294457B2 (en) * 2001-08-07 2007-11-13 Boehringer Ingelheim (Canada) Ltd. Direct binding assay for identifying inhibitors of HCV polymerase
US7098231B2 (en) * 2003-01-22 2006-08-29 Boehringer Ingelheim International Gmbh Viral polymerase inhibitors
US7223785B2 (en) * 2003-01-22 2007-05-29 Boehringer Ingelheim International Gmbh Viral polymerase inhibitors
CN100410387C (zh) * 2003-03-03 2008-08-13 中国药品生物制品检定所 丙型肝炎病毒NS5b聚合酶细胞外分子复制模型及其用途
UY28758A1 (es) * 2004-02-20 2005-09-30 Boehringer Ingelheim Int Inhibidores de polimerasa viral
UY29017A1 (es) * 2004-07-16 2006-02-24 Boehringer Ingelheim Int Inhibidores de polimerasa viral
US20070111216A1 (en) * 2004-09-27 2007-05-17 Epicentre Technologies Methods for identifying polymerase inhibitors
JP5015154B2 (ja) * 2005-08-12 2012-08-29 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング ウイルスポリメラーゼインヒビター
DE602006009220D1 (de) * 2005-12-09 2009-10-29 Hoffmann La Roche Scintillation Proximity Assay
JP2010535155A (ja) * 2007-08-03 2010-11-18 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング ウイルスポリメラーゼ阻害剤
EP2188274A4 (en) * 2007-08-03 2011-05-25 Boehringer Ingelheim Int VIRAL POLYMERASE HEMMER
WO2009076747A1 (en) 2007-12-19 2009-06-25 Boehringer Ingelheim International Gmbh Viral polymerase inhibitors
KR20100118991A (ko) * 2008-02-04 2010-11-08 아이데닉스 파마슈티칼스, 인코포레이티드 매크로시클릭 세린 프로테아제 억제제
US8377962B2 (en) 2009-04-08 2013-02-19 Idenix Pharmaceuticals, Inc. Macrocyclic serine protease inhibitors
US9284307B2 (en) 2009-08-05 2016-03-15 Idenix Pharmaceuticals Llc Macrocyclic serine protease inhibitors
WO2012087769A2 (en) * 2010-12-22 2012-06-28 Indiana Research And Technology Corporation A novel cell-based assay to identify polymerase inhibitors for viral infectious disease
US9353100B2 (en) 2011-02-10 2016-05-31 Idenix Pharmaceuticals Llc Macrocyclic serine protease inhibitors, pharmaceutical compositions thereof, and their use for treating HCV infections
EP3564375A4 (en) * 2016-12-27 2020-12-16 The University Of Tokyo ARNM FUNCTIONALIZATION PROCESS

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NL8302460A (nl) * 1983-07-11 1985-02-01 Philips Nv Werkwijze voor het verminderen van de reflectie van een doorzichtig beeldscherm en beeldscherm met verminderde reflectie.
GB2188924B (en) * 1986-04-08 1990-05-09 Glaverbel Matted glass, process of producing matted glass, photo-voltaic cell incorporating a glass sheet, and process of manufacturing such a cell
FR2670774B1 (fr) * 1990-12-21 1993-09-10 Saint Gobain Vitrage Int Vitrage diffusant.
US5965327A (en) * 1991-12-03 1999-10-12 Asahi Kogaku Kogyo Kaisha Method for manufacturing a master die for a diffusion plate and diffusion manufactured by said method
JP3147481B2 (ja) * 1992-04-21 2001-03-19 松下電器産業株式会社 ガラス製回折格子の成形用金型及びその製造方法及びガラス製回折格子の製造方法
TW388083B (en) * 1995-02-20 2000-04-21 Hitachi Ltd Resist pattern-forming method using anti-reflective layer, resist pattern formed, and method of etching using resist pattern and product formed
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DE19708776C1 (de) * 1997-03-04 1998-06-18 Fraunhofer Ges Forschung Entspiegelungsschicht sowie Verfahren zur Herstellung derselben
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DE19847549C1 (de) * 1998-10-15 2000-06-15 Schott Glas Formgebungswerkzeug mit strukturierter Oberfläche zum Erzeugen von Strukturen auf Glas und seine Anwendung bei der Struktierung von Kanalplatten
DE29819347U1 (de) * 1998-10-30 2000-01-27 Flachglas AG, 90766 Fürth Kalknatron-Silikatglas-Zusammensetzung

Also Published As

Publication number Publication date
WO2002070739A3 (en) 2003-05-30
US20040110126A1 (en) 2004-06-10
NZ528644A (en) 2005-05-27
JP2004520839A (ja) 2004-07-15
IL157280A0 (en) 2004-02-19
HUP0400039A2 (hu) 2004-04-28
EP1370682A2 (en) 2003-12-17
WO2002070739A2 (en) 2002-09-12
CA2439176A1 (en) 2002-09-12
HUP0400039A3 (en) 2004-10-28

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