MY100360A - Microbial gene promoter / operators - Google Patents
Microbial gene promoter / operatorsInfo
- Publication number
- MY100360A MY100360A MYPI87001974A MYPI19871974A MY100360A MY 100360 A MY100360 A MY 100360A MY PI87001974 A MYPI87001974 A MY PI87001974A MY PI19871974 A MYPI19871974 A MY PI19871974A MY 100360 A MY100360 A MY 100360A
- Authority
- MY
- Malaysia
- Prior art keywords
- promoter
- operator
- hybrid
- hgh
- fragment
- Prior art date
Links
- 108700005443 Microbial Genes Proteins 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 4
- 239000013612 plasmid Substances 0.000 abstract 3
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 230000001939 inductive effect Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108091035707 Consensus sequence Proteins 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000010397 one-hybrid screening Methods 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000011144 upstream manufacturing Methods 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
HYBRID PROMOTER/OPERATORS ARE PREPARED AND INSERTED INTO PLASMIDS WHICH ARE USED TO TRANSFORM E. COLI. THEY ARE ARRANGED TO CONTROL EXPRESSION (BY A GENE IN THE PLASMID) OF A DESIRED HETEROLOGOUS PEPTIDE E.G. HUMAN GROWTH HORMONE, HGH. EACH HYBRID PROMOTOR/OPERATOR HAS (A) A PROMOTER/OPERATOR FRAGMENT WHICH IS CAPABLE OF BEING CHEMICALLY INDUCED, AND (B) A FRAGMENT WHICH INCLUDES THE -35 CONSENSUS SEQUENCE AND THE 5'' FLANKING REGION THEREOF OF A PROMOTER HAVING A HIGH SIGNAL STRENGTH. THE FRAGMENTS (A) AND (B) ARE APPROPRIATELY SPACED TO FORM AN OPERABLE HYBRID PROMOTER WHICH IS BOTH INDUCIBLE AND OF HIGH SIGNAL STRENGTH (PROPERTIES NOT NATURALLY FOUND TOGETHER). E.G. ONE HYBRID OPERATOR/PROMOTER HAS A SEQUENCE FROM UPSTREAM OF POSITION -22 OF THE TRP PROMOTER (OF HIGH STRENGTH) COUPLED TO A SEQUENCE DOWNSTREAM FROM POSITION -19 OF THE LAC PROMOTER/OPERATOR (INDUCIBLE). THIS WAS USED TO CONTROL A GENE FOR HGH IN A PLASMID PHGH807TACI. E. COLI WAS TRANSFORMED THEREWITH AND CULTURED. PRODUCTION OF HGH (RAPID OWING TO THE STRONG PROMOTER FRAGMENT) WAS INDUCED BY ADDITION OF ISOPROPYL-(BETA)-D-GALACTOSIDE TO THE CULTURE MEDIUM.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26430681A | 1981-05-18 | 1981-05-18 | |
| US32817481A | 1981-12-07 | 1981-12-07 | |
| US06/338,397 US4551433A (en) | 1981-05-18 | 1982-01-11 | Microbial hybrid promoters |
| EP19820302532 EP0067540B1 (en) | 1981-05-18 | 1982-05-18 | Microbial gene promoter/operators |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MY100360A true MY100360A (en) | 1990-08-28 |
Family
ID=27440427
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MYPI87001974A MY100360A (en) | 1981-05-18 | 1987-09-26 | Microbial gene promoter / operators |
Country Status (2)
| Country | Link |
|---|---|
| AT (1) | ATE27181T1 (en) |
| MY (1) | MY100360A (en) |
-
1982
- 1982-05-18 AT AT82302532T patent/ATE27181T1/en active
-
1987
- 1987-09-26 MY MYPI87001974A patent/MY100360A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE27181T1 (en) | 1987-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IE821174L (en) | Microbial gene promoter/operator | |
| US4727028A (en) | Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof | |
| CA1267099A (en) | Secretion of correctly processed human growth hormone in e.coli and pseudomonas | |
| DE69634819T2 (en) | REGULATED AUTOKRINES GROWTH OF MAMMALIAN CELLS | |
| IE53521B1 (en) | Recombinant dna cloning vectors and eukaryotic and prokaryotic transformants thereof | |
| EP0001930A3 (en) | Method for polypeptide production involving expression of a heterologous gene, recombinant microbial cloning vehicle containing said gene and bacterial culture transformed by said cloning vehicle | |
| AU575784B2 (en) | Swine growth hormone-like polypeptides | |
| DE3379959D1 (en) | Process for the preparation of interferon and expression vehicles therefor | |
| GB8717430D0 (en) | Recombinant dna product | |
| IL80022A (en) | Cloned human basic fibroblast growth factor, methods for its preparation, compositions containing it, dna sequences encoding therefor and vectors and host cells containing said dna | |
| UA8035A1 (en) | Method for producing human interferon alpha | |
| DE69010096T2 (en) | Expression and secretion of mature human beta-interleukin-1 in Bacillus subtilis and means and methods for their implementation. | |
| MY100360A (en) | Microbial gene promoter / operators | |
| EP0651803B1 (en) | Protein expression system | |
| DE3585415D1 (en) | RECOMBINANT PLASMIDE, BACTERIA CONTAINING THIS RECOMBINANT PLASMIDE, METHOD FOR PRODUCING FERMENTED FOODSTUFFS, ANIMAL FEED, COMPONENTS OF THE SAME OR PROTEINS USING THESE BACTERIA. | |
| DE3750348T2 (en) | Process for the preparation of peptides, recombinant plasmid to be used for this and myeloma cells transformed with this plasmid. | |
| DE3853479T2 (en) | BALANCED, CONSTITUTIVE, INDUCIBLE TRANSCRIPTION SYSTEM. | |
| DE3887635T2 (en) | Process for the production of products from foreign genes. | |
| DE3650692T2 (en) | Recombinant ricin toxin | |
| Šebo et al. | Delineation of the minimal portion of the Bacillus sphaericus 1593M toxin required for the expression of larvicidal activity | |
| WO1994002609A1 (en) | Protein expression system | |
| US5395761A (en) | Plasmid pHKY334, an expression vector for EK-BGH and host cells transformed therewith | |
| Masuda et al. | Synthesis of enzymatically active mouse submandibular gland renin in Escherichia coli | |
| AU704095B2 (en) | Regulated autocrine growth of mammalian cells | |
| DE3485883T2 (en) | RECOMBINANT MATERIALS AND METHOD FOR PRODUCING HUMAN CONNECTIVE TISSUE ACTIVATING PEPTIDES III AND THEIR ANALOGS. |