Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotronics CorpfiledCriticalBiotronics Corp
Priority to MYPI94001296ApriorityCriticalpatent/MY111893A/en
Publication of MY111893ApublicationCriticalpatent/MY111893A/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
A METHOD FOR DETECTING A TARGET NUCLEIC ACID, WHICH METHOD INCLUDES THE STEPS OF (I) AMPLIFYING THE TARGET NUCLEIC ACID TO OBTAIN AN AMPLIFICATION PRODUCT USING A POLYMERASE, A FIRST PRIMER WITH OR WITHOUT A SEGMENT NONCONTIGUOUS TO A FIRST PRIMING SEQUENCE, AND A SECOND PRIMER WITH OR WITHOUT SEGMENT NONCONTIGUOUS TO A SECOND PRIMING SEQUENCE IN THE PRESENCE OF AN OLIGONUCLEOTIDE WHICH IS INCAPABLE OF ACTING AS A PRIMER FOR THE POLYMERASE, WHEREIN THE OLIGONUCLEOTIDE HAS AT LEAST 5 CONSECUTIVE NUCLEOTIDES FULLY COMPLEMENTARY TO AT LEAST 5 CONSECUTIVE NUCLEOTIDES OF THE FIRST PRIMER; AND (II) DETECTING THE PRESENCE OF THE TARGET NUCLEIC ACID BY MONITORING THE AMPLIFICATION THEREOF.(FIG. 2)
MYPI94001296A1994-05-201994-05-20Method for detecting a target nucleic acid
MY111893A
(en)