MY144323A - Method for linking sequences of interest - Google Patents
Method for linking sequences of interestInfo
- Publication number
- MY144323A MY144323A MYPI20043820A MYPI20043820A MY144323A MY 144323 A MY144323 A MY 144323A MY PI20043820 A MYPI20043820 A MY PI20043820A MY PI20043820 A MYPI20043820 A MY PI20043820A MY 144323 A MY144323 A MY 144323A
- Authority
- MY
- Malaysia
- Prior art keywords
- sequences
- extension
- pcr
- interest
- multiplex overlap
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000003757 reverse transcription PCR Methods 0.000 abstract 2
- 108060003951 Immunoglobulin Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091008874 T cell receptors Proteins 0.000 abstract 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 abstract 1
- 102000018358 immunoglobulin Human genes 0.000 abstract 1
- 229940072221 immunoglobulins Drugs 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
MULTIPLEX OVERLAP-EXTENSION RT-PCR PROVIDES AN EFFICIENT METHOD OF LINKING TWO OR MORE NUCLEOTIDE SEQUENCES ENCODING FOR DOMAINS OR SUBUNITS OF A HETEROMERIC PROTEIN, IN A SINGLE REACTION. ESPECIALLY, THE LINKAGE OF VARIABLE REGION ENCODING SEQUENCES FROM E.G. IMMUNOGLOBULINS , T CELL RECEPTOR IS EASED WITH THE METHOD OF THE PRESENT INVENTION. THIS ALLOW FOR A MORE EFFICIENT WAY OF GENERATING LIBRARIES OF VARIABLE REGIONENCODING SEQUENCES. THE CAPABILITY TO PERFORM THE MULTIPLEX OVERLAP-EXTENSION RT-PCR USING TEMPLATE DERIVED FROM AN ISOLATED SINGLE CELL ENABLES THE GENERATION OF COGNATE PAIR LIBRARIES IN A HIGH-THROUGHPUT FORMAT.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50458903P | 2003-09-18 | 2003-09-18 | |
| DKPA200301867 | 2003-12-17 | ||
| DKPA200400782 | 2004-05-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MY144323A true MY144323A (en) | 2011-08-29 |
Family
ID=40257283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MYPI20043820A MY144323A (en) | 2003-09-18 | 2004-09-17 | Method for linking sequences of interest |
Country Status (3)
| Country | Link |
|---|---|
| AR (1) | AR045756A1 (en) |
| MY (1) | MY144323A (en) |
| PA (1) | PA8612801A1 (en) |
-
2004
- 2004-09-17 PA PA20048612801A patent/PA8612801A1/en unknown
- 2004-09-17 AR ARP040103343A patent/AR045756A1/en active IP Right Grant
- 2004-09-17 MY MYPI20043820A patent/MY144323A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PA8612801A1 (en) | 2005-03-28 |
| AR045756A1 (en) | 2005-11-09 |
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