MY177150A - Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof - Google Patents

Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof

Info

Publication number
MY177150A
MY177150A MYPI2012000874A MYPI2012000874A MY177150A MY 177150 A MY177150 A MY 177150A MY PI2012000874 A MYPI2012000874 A MY PI2012000874A MY PI2012000874 A MYPI2012000874 A MY PI2012000874A MY 177150 A MY177150 A MY 177150A
Authority
MY
Malaysia
Prior art keywords
discloses
dpsc
icas
isolation
expansion
Prior art date
Application number
MYPI2012000874A
Inventor
Govindasamy Vijayendran
R Bhonde Ramesh
Totey Satish
Das Kumar Anjan
Original Assignee
Stempeutics Res Malaysia Sdn Bhd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stempeutics Res Malaysia Sdn Bhd filed Critical Stempeutics Res Malaysia Sdn Bhd
Publication of MY177150A publication Critical patent/MY177150A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The present invention discloses highly reproducible and consistent method for large scale production of high quality clinica-l -grade DPSCs in a short time. The present method of DPSC production is economical and produces cells in commercial quantities for use in autologous and allogeneic transplantation therapy. It also discloses a transport medium composition for preservation and protection of the dental pulp. It further discloses a method for producing islet-like cell aggregates (ICAs) from DPSC using a 3 step protocol and using specific differentiation composition. The ICAs obtained is used as pharmaceutical composition for treating type-I diabetic conditions and also for screening novel anti-diabetic compounds.
MYPI2012000874A 2011-02-28 2012-02-27 Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof MY177150A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2011000923 2011-02-28

Publications (1)

Publication Number Publication Date
MY177150A true MY177150A (en) 2020-09-08

Family

ID=46757399

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2012000874A MY177150A (en) 2011-02-28 2012-02-27 Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof

Country Status (2)

Country Link
MY (1) MY177150A (en)
WO (1) WO2012117333A1 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
US9096832B2 (en) 2007-07-31 2015-08-04 Lifescan, Inc. Differentiation of human embryonic stem cells
ATE523585T1 (en) 2007-11-27 2011-09-15 Lifescan Inc DIFFERENTIATION OF HUMAN EMBRYONAL STEM CELLS
CA2959401C (en) 2008-02-21 2021-12-07 Centocor Ortho Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
KR101651661B1 (en) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 Differentiation of pluripotent stem cells
MX2011004563A (en) 2008-10-31 2011-06-01 Centocor Ortho Biotech Inc Differentiation of human embryonic stem cells to the pancreatic endocrine lineage.
CN102272291B (en) 2008-10-31 2018-01-16 詹森生物科技公司 Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
PL2366021T3 (en) 2008-11-20 2017-12-29 Janssen Biotech, Inc. Pluripotent stem cell culture on micro-carriers
PL2366022T3 (en) 2008-11-20 2016-11-30 Methods and compositions for cell attachment and cultivation on planar substrates
KR20170118969A (en) 2009-07-20 2017-10-25 얀센 바이오테크 인코포레이티드 Differentiation of human embryonic stem cells
AU2010333839C1 (en) 2009-12-23 2016-07-21 Janssen Biotech Inc. Differentiation of human embryonic stem cells
CN107189979B (en) 2010-03-01 2021-05-04 詹森生物科技公司 Methods of purifying cells derived from pluripotent stem cells
KR101986176B1 (en) 2010-05-12 2019-06-05 얀센 바이오테크 인코포레이티드 Differentiation of human embryonic stem cells
WO2012030539A2 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
AU2011296381B2 (en) 2010-08-31 2016-03-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US9388386B2 (en) 2011-12-22 2016-07-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
CA2866590A1 (en) 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
WO2013184888A1 (en) 2012-06-08 2013-12-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
WO2014105543A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
RU2684215C2 (en) 2012-12-31 2019-04-04 Янссен Байотек, Инк. Method for obtaining pancreatic endocrine cells (versions) and method for increasing output of beta cells
RU2687379C2 (en) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Suspending and clustering of human pluripotent cells with the purpose of their differentiation in pancreatic endocrine cells
KR20180128529A (en) * 2013-11-01 2018-12-03 얀센 바이오테크 인코포레이티드 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
JP6588969B2 (en) 2014-05-16 2019-10-09 ヤンセン バイオテツク，インコーポレーテツド Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
MY166810A (en) 2014-08-20 2018-07-23 Hygieia Innovation Sdn Bhd Isolation, expansion and characterization of precursor/stem cells from dental tissues
SG11201806561UA (en) * 2015-10-02 2018-09-27 Elixir Biopharma Sdn Bhd Method of isolating mesenchymal stromal cells and applications for tissue engineering
JP6705911B2 (en) 2016-04-14 2020-06-03 ヤンセン バイオテツク，インコーポレーテツド Differentiation of pluripotent stem cells into midgut endoderm cells
US20200270579A1 (en) * 2017-02-03 2020-08-27 Kyushu University, National University Corporation Method for preparing dental pulp stem cells from cells derived from dental pulp tissue
MY198403A (en) * 2019-06-12 2023-08-28 Cryocord Sdn Bhd Production of Therapeutics Potential Mesenchymal Stem Cells
CN111727961B (en) * 2020-08-10 2020-12-01 医微细胞生物技术(广州)有限公司 A kind of dental pulp stem cell cryopreservation solution and cryopreservation method thereof
CN111909898B (en) * 2020-08-17 2021-03-23 深圳市茵冠生物科技有限公司 Separation and amplification method of human dental pulp stem cells and application thereof
CN115521906A (en) * 2021-06-24 2022-12-27 东莞宣冠干细胞再生医学有限公司 A method for preparing high-quality human dental pulp stem cells
WO2024075145A1 (en) * 2022-10-03 2024-04-11 キッズウェル・バイオ株式会社 Novel dental pulp stem cell population
EP4600350A1 (en) * 2022-10-03 2025-08-13 Kidswell Bio Corporation Novel dental pulp stem cell population

Also Published As

Publication number Publication date
WO2012117333A1 (en) 2012-09-07

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