MY184600A - Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus - Google Patents

Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus

Info

Publication number
MY184600A
MY184600A MYPI2012700037A MYPI2012700037A MY184600A MY 184600 A MY184600 A MY 184600A MY PI2012700037 A MYPI2012700037 A MY PI2012700037A MY PI2012700037 A MYPI2012700037 A MY PI2012700037A MY 184600 A MY184600 A MY 184600A
Authority
MY
Malaysia
Prior art keywords
real
virus
kit
seq
necrosis virus
Prior art date
Application number
MYPI2012700037A
Inventor
Yasmin Binti Othman Rofina
Beng Chu Kua
A/P Bhassu Subha
Lay Khoon Too
Original Assignee
Univ Malaya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Malaya filed Critical Univ Malaya
Priority to MYPI2012700037A priority Critical patent/MY184600A/en
Priority to PCT/MY2013/000004 priority patent/WO2013129906A1/en
Priority to CN201380011125.8A priority patent/CN104204231A/en
Priority to IN7968DEN2014 priority patent/IN2014DN07968A/en
Publication of MY184600A publication Critical patent/MY184600A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for quantitatively detecting infectious hypodermal and haematopoietic necrosis virus (IHHNV) in a biological sample, comprising the steps of obtaining a primer pair having nucleotide sequences set forth in SEQ ID NO: 1 and SEQ ID NO: 2, and a fluorescent probe having a nucleotide sequence set forth in SEQ ID NO: 3; amplifying a non-structural protein coding region of the virus in the biological sample by a real-time polymerase chain reaction (PCR) using the primer pair and the fluorescent probe; and determining the presence and quantity of the virus in the biological sample with reference to a positive control plasmid.
MYPI2012700037A 2012-02-27 2012-02-27 Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus MY184600A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
MYPI2012700037A MY184600A (en) 2012-02-27 2012-02-27 Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus
PCT/MY2013/000004 WO2013129906A1 (en) 2012-02-27 2013-01-15 Real-time viral detection method and kit for infectious hypodermaland haematopoietic necrosis virus
CN201380011125.8A CN104204231A (en) 2012-02-27 2013-01-15 Real-time viral detection method and kit for infectious hypodermaland haematopoietic necrosis virus
IN7968DEN2014 IN2014DN07968A (en) 2012-02-27 2013-01-15

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2012700037A MY184600A (en) 2012-02-27 2012-02-27 Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus

Publications (1)

Publication Number Publication Date
MY184600A true MY184600A (en) 2021-04-07

Family

ID=49083015

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2012700037A MY184600A (en) 2012-02-27 2012-02-27 Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus

Country Status (4)

Country Link
CN (1) CN104204231A (en)
IN (1) IN2014DN07968A (en)
MY (1) MY184600A (en)
WO (1) WO2013129906A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108531657A (en) * 2018-05-09 2018-09-14 鲁东大学 The fluorescence quantitative PCR detection primer sets and detection kit of prawn infectious subcutaneous and haematopoietic necrosis virus
CN112176111A (en) * 2020-11-05 2021-01-05 北京市水产技术推广站 Primer, probe, kit and detection method for detecting infectious pancreatic necrosis virus
CN116200539A (en) * 2022-12-19 2023-06-02 中国海洋大学三亚海洋研究院 ERA technology-based method for rapidly detecting infectious subcutaneous and hematopoietic necrosis viruses

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410549A (en) * 2001-09-21 2003-04-16 中国科学院南海海洋研究所 Fast detection prawn infectious subcutaneous and hemopoiesis tissue necrosis virus reagent box
CN100351630C (en) * 2005-10-23 2007-11-28 王学海 Kit for diagnosing FQ-PCR of prawn IHHNV and its testing method
CN101506387B (en) * 2006-08-24 2014-04-09 纳幕尔杜邦公司 Sequences used to diagnose shrimp pathogens
CN100460519C (en) * 2006-08-29 2009-02-11 中国科学院南海海洋研究所 Kit and method for detecting shrimp infectious subcutaneous and hematopoietic necrosis virus
CN101643793A (en) * 2009-08-27 2010-02-10 中国科学院南海海洋研究所 Reagent kit for testing infectious hypodermic and hematopoietic necrosis viruses of prawns, and testing method thereof
CN101792817B (en) * 2009-11-30 2012-07-25 浙江大学 Detection method of prawn IHHNV (infectious hypodermal and hematopoietic necrosis virus) and used nucleic acid isothermal amplification detection kit

Also Published As

Publication number Publication date
IN2014DN07968A (en) 2015-05-01
WO2013129906A1 (en) 2013-09-06
CN104204231A (en) 2014-12-10

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