MY186947A - Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways - Google Patents

Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways

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Publication number
MY186947A
MY186947A MYPI2017702761A MYPI2017702761A MY186947A MY 186947 A MY186947 A MY 186947A MY PI2017702761 A MYPI2017702761 A MY PI2017702761A MY PI2017702761 A MYPI2017702761 A MY PI2017702761A MY 186947 A MY186947 A MY 186947A
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MY
Malaysia
Prior art keywords
serine
production
present
genetically engineered
genetically modified
Prior art date
Application number
MYPI2017702761A
Inventor
Hemanshu Mundhada
Alex Toftgaard Nielsen
Original Assignee
Cysbio Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cysbio Aps filed Critical Cysbio Aps
Publication of MY186947A publication Critical patent/MY186947A/en

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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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Abstract

The present invention generally relates to the microbiological industry, and specifically to the production of L-serine using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.
MYPI2017702761A 2015-01-27 2016-01-27 Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways MY186947A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15152643 2015-01-27
PCT/EP2016/051701 WO2016120326A1 (en) 2015-01-27 2016-01-27 Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways

Publications (1)

Publication Number Publication Date
MY186947A true MY186947A (en) 2021-08-26

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MYPI2017702761A MY186947A (en) 2015-01-27 2016-01-27 Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways
MYPI2017702765A MY186949A (en) 2015-01-27 2016-01-27 Genetically modified microorganisms having improved tolerance towards l-serine

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MYPI2017702765A MY186949A (en) 2015-01-27 2016-01-27 Genetically modified microorganisms having improved tolerance towards l-serine

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US (3) US10513682B2 (en)
EP (3) EP3250700B1 (en)
JP (2) JP6835725B2 (en)
CN (2) CN107406864B (en)
CA (2) CA2975011C (en)
DK (2) DK3250700T3 (en)
ES (2) ES2807448T3 (en)
HU (1) HUE050530T2 (en)
LT (1) LT3250701T (en)
MY (2) MY186947A (en)
PL (1) PL3250700T3 (en)
SI (1) SI3250701T1 (en)
WO (2) WO2016120326A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2807448T3 (en) * 2015-01-27 2021-02-23 Cysbio Aps Method for the production of L-serine using genetically engineered microorganisms deficient for serine degradation pathways
US10858676B2 (en) * 2017-05-22 2020-12-08 Ajinomoto Co., Inc. Method for producing objective substance
CN110229853B (en) * 2019-07-02 2021-06-01 武汉轻工大学 Preparation method of L-serine
CN111019877B (en) * 2019-12-31 2022-04-19 浙江工业大学 A kind of L-cysteine-producing genetically engineered bacteria, construction method and application
EP3960879A1 (en) * 2020-09-01 2022-03-02 Metabolic Explorer Microorganism and method for the improved production of alanine
WO2024013258A1 (en) * 2022-07-15 2024-01-18 Cysbio Aps Production of cysteine or cystine from serine in fermentation medium
WO2024028428A1 (en) 2022-08-04 2024-02-08 Metabolic Explorer Microorganism and method for the improved production of serine and/or cysteine
CN120476201A (en) 2022-10-20 2025-08-12 赛斯生物私营有限责任公司 Genetically modified host cells producing L-serine
KR20240086804A (en) * 2022-12-08 2024-06-19 씨제이제일제당 (주) Microorganisms for producing purine nucleotides and process for producing purine nucleotides using the same
EP4649144A1 (en) 2023-01-09 2025-11-19 Evonik Operations GmbH Fermentative production guanidinoacetic acid (gaa) from serine using a microorganism having an enhanced l-serine hydroxymethyltransferase activity
WO2024149617A1 (en) 2023-01-09 2024-07-18 Evonik Operations Gmbh Fermentative production guanidinoacetic acid (gaa) from serine by attenuating l serine ammonia lyase activity in microorganisms
WO2024224355A1 (en) 2023-04-26 2024-10-31 Cysbio Aps A low dose formulation of l-serine for liver health and fatty liver diseases
KR102869970B1 (en) * 2023-06-23 2025-10-14 씨제이제일제당 (주) A polynucleotide comprising a mutated RBS nucleotide sequence, a microorganism comprising the polynucleotide, and a method for producing glycine using the same
CN120005781A (en) 2023-11-14 2025-05-16 赛斯生物私人有限公司 Expression of biomolecules with improved promoters and TIRs
CN119662748A (en) * 2024-12-30 2025-03-21 诸城市浩天药业有限公司 L-serine production method

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JPH03501682A (en) 1988-10-25 1991-04-18 味の素株式会社 Escherichia coli BKIIM strain B-3996 as an L-threonine producer
JPH07108228B2 (en) 1990-10-15 1995-11-22 味の素株式会社 Temperature sensitive plasmid
CN1103819C (en) 1994-06-14 2003-03-26 味之素株式会社 α-ketoglutarate dehydrogenase gene
JP2000262288A (en) 1999-03-16 2000-09-26 Ajinomoto Co Inc Temperature sensitive plasmid of coryneform bacteria
DE10311399A1 (en) * 2003-03-13 2004-09-23 Forschungszentrum Jülich GmbH New nucleic acid encoding inactive L-serine dehydratase, useful for improving bacterial synthesis of L-serine, is a deleted or mutated form of the wild-type sequence
RU2275424C2 (en) * 2003-12-05 2006-04-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Method for preparing l-threonine by using bacterium belonging to genus escherichia
DE05793824T1 (en) * 2004-10-13 2008-02-07 Mitsui Chemicals, Inc. DNA encoding a novel enzyme encoding D-serine synapsease activity, method for producing the same, and method for producing D-serine using the same
DE102005049527B4 (en) * 2005-10-17 2013-05-08 Forschungszentrum Jülich GmbH Process for the preparation of L-serine, gene sequence, vectors and microorganism
WO2007144018A1 (en) * 2006-06-12 2007-12-21 Metabolic Explorer Ethanolamine production by fermentation
WO2008010565A2 (en) * 2006-07-19 2008-01-24 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family
EP2395096B1 (en) * 2009-02-09 2014-04-09 Kyowa Hakko Bio Co., Ltd. Process for producing L-amino acids
MY172018A (en) * 2009-12-30 2019-11-12 Metabolic Explorer Sa Strains and method for the production of methionine
EP2588598B1 (en) * 2010-07-02 2016-03-09 Metabolic Explorer Method for the preparation of hydroxy acids
CN102703371A (en) * 2012-07-03 2012-10-03 天津科技大学 Escherichia coli engineering bacteria for producing L-serine with high yield and fermentation method for engineering bacteria
ES2807448T3 (en) * 2015-01-27 2021-02-23 Cysbio Aps Method for the production of L-serine using genetically engineered microorganisms deficient for serine degradation pathways

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