MY190479A - A two-dimensional electrophoresis method for separation of protein compounds - Google Patents

A two-dimensional electrophoresis method for separation of protein compounds

Info

Publication number
MY190479A
MY190479A MYPI2015702656A MYPI2015702656A MY190479A MY 190479 A MY190479 A MY 190479A MY PI2015702656 A MYPI2015702656 A MY PI2015702656A MY PI2015702656 A MYPI2015702656 A MY PI2015702656A MY 190479 A MY190479 A MY 190479A
Authority
MY
Malaysia
Prior art keywords
fractions
separation
eluted
liquid phase
dimension
Prior art date
Application number
MYPI2015702656A
Inventor
Nazri Bin Ismail Mohd
Soon Hong Kwan
Original Assignee
Univ Sains Malaysia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sains Malaysia filed Critical Univ Sains Malaysia
Priority to MYPI2015702656A priority Critical patent/MY190479A/en
Priority to PCT/MY2016/050030 priority patent/WO2017026890A1/en
Publication of MY190479A publication Critical patent/MY190479A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)

Abstract

The present invention relates to a method of separating protein compounds from a sample based on inverted two-dimensional polyacrylamide gel electrophoresis (i2D- PAGE) comprising the steps of: electrophoretically separating compounds from a sample loaded into a polyacrylamide gel tube in a first dimension into discrete fractions of different molecular weights, in which the separation is conducted using gel eluted liquid fraction entrapment electrophoresis whereby each of the fractions being eluted from the gel tube is trapped in a subsequent liquid phase; collecting the liquid phase containing each of the eluted fractions; and subjecting the liquid phase to a second separation in a second dimension using an isoelectric focusing medium in the presence of electric field, in which each of the fractions are resolved according to its isoelectric point.
MYPI2015702656A 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds MY190479A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2015702656A MY190479A (en) 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds
PCT/MY2016/050030 WO2017026890A1 (en) 2015-08-13 2016-05-06 A two-dimensional electrophoresis method for separation of protein compounds

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2015702656A MY190479A (en) 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds

Publications (1)

Publication Number Publication Date
MY190479A true MY190479A (en) 2022-04-22

Family

ID=57983959

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2015702656A MY190479A (en) 2015-08-13 2015-08-13 A two-dimensional electrophoresis method for separation of protein compounds

Country Status (2)

Country Link
MY (1) MY190479A (en)
WO (1) WO2017026890A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105527332B (en) * 2016-01-18 2018-05-22 云南省农业科学院花卉研究所 The extraction of lily total protein and the acquisition methods of dielectrophoresis differential protein collection of illustrative plates

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009046526A1 (en) * 2007-10-09 2009-04-16 Dalhousie University Apparatus for purifying molecules

Also Published As

Publication number Publication date
WO2017026890A1 (en) 2017-02-16

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