NL1002275C2 - Modificatie van polysacchariden. - Google Patents

Modificatie van polysacchariden. Download PDF

Info

Publication number: NL1002275C2
Authority: NL; Netherlands
Prior art keywords: sugar group; enzyme; fructosyl; transferase; substrate
Prior art date: 1996-02-07

Application number

NL1002275A

Other languages

English (en)

Dutch (nl)

Inventor

Stefanus Cornelis Hendrik Turk

Josephus Christianus Smeekens

Nathalie Gerrits

Petrus Jacobus Weisbeek

Original Assignee

Have D J Van Der Bv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-02-07

Filing date

1996-02-07

Publication date

1997-08-08

1996-02-07 Application filed by Have D J Van Der Bv filed Critical Have D J Van Der Bv

1996-02-07 Priority to NL1002275A priority Critical patent/NL1002275C2/nl

1997-02-07 Priority to EP97902728A priority patent/EP1015562B1/de

1997-02-07 Priority to DK97902728T priority patent/DK1015562T3/da

1997-02-07 Priority to PCT/NL1997/000039 priority patent/WO1997029186A1/en

1997-02-07 Priority to CA2245818A priority patent/CA2245818C/en

1997-02-07 Priority to AU16745/97A priority patent/AU732087B2/en

1997-02-07 Priority to DE69738085T priority patent/DE69738085T2/de

1997-02-07 Priority to AT97902728T priority patent/ATE371726T1/de

1997-08-08 Application granted granted Critical

1997-08-08 Publication of NL1002275C2 publication Critical patent/NL1002275C2/nl

2002-03-29 Priority to US10/112,797 priority patent/US6833491B2/en

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
- C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/08—Dextran
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Nutrition Science (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Plant Pathology (AREA)
Medicinal Chemistry (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Jellies, Jams, And Syrups (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

NL1002275A 1996-02-07 1996-02-07 Modificatie van polysacchariden. NL1002275C2 (nl)

Priority Applications (9)

Application Number	Priority Date	Filing Date	Title
NL1002275A NL1002275C2 (nl)	1996-02-07	1996-02-07	Modificatie van polysacchariden.
EP97902728A EP1015562B1 (de)	1996-02-07	1997-02-07	Modifikation von polysacchariden
DK97902728T DK1015562T3 (da)	1996-02-07	1997-02-07	Modifikation af polysaccharider
PCT/NL1997/000039 WO1997029186A1 (en)	1996-02-07	1997-02-07	Modification of polysaccharides
CA2245818A CA2245818C (en)	1996-02-07	1997-02-07	Modification of polysaccharides
AU16745/97A AU732087B2 (en)	1996-02-07	1997-02-07	Modification of polysaccharides
DE69738085T DE69738085T2 (de)	1996-02-07	1997-02-07	Modifikation von polysacchariden
AT97902728T ATE371726T1 (de)	1996-02-07	1997-02-07	Modifikation von polysacchariden
US10/112,797 US6833491B2 (en)	1996-02-07	2002-03-29	Modification of polysaccharides

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
NL1002275A NL1002275C2 (nl)	1996-02-07	1996-02-07	Modificatie van polysacchariden.
NL1002275		1996-02-07

Publications (1)

Publication Number	Publication Date
NL1002275C2 true NL1002275C2 (nl)	1997-08-08

Family

ID=19762266

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL1002275A NL1002275C2 (nl)	1996-02-07	1996-02-07	Modificatie van polysacchariden.

Country Status (8)

Country	Link
EP (1)	EP1015562B1 (de)
AT (1)	ATE371726T1 (de)
AU (1)	AU732087B2 (de)
CA (1)	CA2245818C (de)
DE (1)	DE69738085T2 (de)
DK (1)	DK1015562T3 (de)
NL (1)	NL1002275C2 (de)
WO (1)	WO1997029186A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6284479B1 (en)	1995-06-07	2001-09-04	Pioneer Hi-Bred International, Inc.	Substitutes for modified starch and latexes in paper manufacture
US6127602A (en) *	1995-06-07	2000-10-03	Pioneer Hi-Bred International, Inc.	Plant cells and plants transformed with streptococcus mutans genes encoding wild-type or mutant glucosyltransferase D enzymes
US5712107A (en) *	1995-06-07	1998-01-27	Pioneer Hi-Bred International, Inc.	Substitutes for modified starch and latexes in paper manufacture
US6087559A (en) *	1995-06-07	2000-07-11	Pioneer Hi-Bred International, Inc.	Plant cells and plants transformed with Streptococcus mutans genes encoding wild-type or mutant glucosyltransferase B enzymes
DE19708774A1 (de)	1997-03-04	1998-09-17	Max Planck Gesellschaft	Nucleinsäuremoleküle codierend Enzyme die Fructosylpolymeraseaktivität besitzen
WO1999057258A1 (en) *	1998-05-01	1999-11-11	The Procter & Gamble Company	Laundry detergent and/or fabric care compositions comprising a modified transferase
DE19846635A1 (de) *	1998-10-09	2000-05-11	Planttec Biotechnologie Gmbh	Verfahren zur Herstellung von alpha-1,6-verzweigten alpha-1,4-Glucanen sowie Nucleinsäuremoleküle codierend ein Verzweigungsenzym aus Bakterien der Gattung Neisseria
AU765131C (en) *	1998-10-09	2007-03-15	Bayer Cropscience Aktiengesellschaft	Nucleic acid molecules encoding a branching enzyme from bacteria of the genus neisseria as well as methods for the production of alpha-1 6-branched alpha-1 4- glucans
CN1211483C (zh)	1999-03-29	2005-07-20	诺沃奇梅兹有限公司	具有分支酶活性的多肽及其编码核酸
EP1584688A3 (de) *	1999-11-18	2006-07-19	E.I. du Pont de Nemours and Company	Gemisch aus Stärke und Fruktoologosaccharide
US6791015B2 (en)	2000-10-30	2004-09-14	E. I. Du Pont De Nemours And Company	Fructan biosynthetic enzymes
US7629013B2 (en)	2005-10-17	2009-12-08	Kraft Foods Global Brands Llc	Partial fat replacement in flat bread
EP2140009A2 (de) *	2007-03-29	2010-01-06	CBD Technologies Ltd.	Transgene pflanzen mit löslichen zellwand-polysacchariden

Citations (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1989012386A1 (en) *	1988-06-21	1989-12-28	Calgene, Inc.	Methods and compositions for altering physical characteristics of fruit and fruit products
DE4227061A1 (de) *	1992-08-12	1994-02-17	Inst Genbiologische Forschung	DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
WO1994014970A1 (en) *	1992-12-28	1994-07-07	Stichting Scheikundig Onderzoek In Nederland	Method for obtaining transgenic plants showing a modified fructan pattern
DE4330960A1 (de) *	1993-09-09	1995-03-16	Inst Genbiologische Forschung	Kombination von DNA-Sequenzen, die in Pflanzenzellen und Pflanzen die Bildung hochgradig amylosehaltiger Stärke ermöglichen, Verfahren zur Herstellung dieser Pflanzen und die daraus erhaltbare modifizierte Stärke
DE4420223C1 (de) *	1994-06-06	1995-05-04	Inst Genbiologische Forschung	Verfahren zur Kombination der intrazellulären Polyhydroxyalkanoat-Synthese in Mikroorganismen mit einer extrazellulären Polysaccharid-Synthese
WO1995013389A1 (en) *	1993-11-09	1995-05-18	E.I. Du Pont De Nemours And Company	Transgenic fructan accumulating crops and methods for their production
WO1996001904A1 (en) *	1994-07-08	1996-01-25	Stichting Scheikundig Onderzoek In Nederland	Production of oligosaccharides in transgenic plants

1996
- 1996-02-07 NL NL1002275A patent/NL1002275C2/nl not_active IP Right Cessation
1997
- 1997-02-07 DK DK97902728T patent/DK1015562T3/da active
- 1997-02-07 AU AU16745/97A patent/AU732087B2/en not_active Expired
- 1997-02-07 WO PCT/NL1997/000039 patent/WO1997029186A1/en not_active Ceased
- 1997-02-07 DE DE69738085T patent/DE69738085T2/de not_active Expired - Lifetime
- 1997-02-07 CA CA2245818A patent/CA2245818C/en not_active Expired - Lifetime
- 1997-02-07 EP EP97902728A patent/EP1015562B1/de not_active Expired - Lifetime
- 1997-02-07 AT AT97902728T patent/ATE371726T1/de not_active IP Right Cessation

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1989012386A1 (en) *	1988-06-21	1989-12-28	Calgene, Inc.	Methods and compositions for altering physical characteristics of fruit and fruit products
DE4227061A1 (de) *	1992-08-12	1994-02-17	Inst Genbiologische Forschung	DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
WO1994014970A1 (en) *	1992-12-28	1994-07-07	Stichting Scheikundig Onderzoek In Nederland	Method for obtaining transgenic plants showing a modified fructan pattern
DE4330960A1 (de) *	1993-09-09	1995-03-16	Inst Genbiologische Forschung	Kombination von DNA-Sequenzen, die in Pflanzenzellen und Pflanzen die Bildung hochgradig amylosehaltiger Stärke ermöglichen, Verfahren zur Herstellung dieser Pflanzen und die daraus erhaltbare modifizierte Stärke
WO1995013389A1 (en) *	1993-11-09	1995-05-18	E.I. Du Pont De Nemours And Company	Transgenic fructan accumulating crops and methods for their production
DE4420223C1 (de) *	1994-06-06	1995-05-04	Inst Genbiologische Forschung	Verfahren zur Kombination der intrazellulären Polyhydroxyalkanoat-Synthese in Mikroorganismen mit einer extrazellulären Polysaccharid-Synthese
WO1996001904A1 (en) *	1994-07-08	1996-01-25	Stichting Scheikundig Onderzoek In Nederland	Production of oligosaccharides in transgenic plants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NAKANO,Y.J., ET AL.: "MECHANISM OF STREPTOCOCCUS MUTANS GLUCOSYLTRANSFERASES: HYBRID-ENZYME ANALYSIS", JOURNAL OF BACTERIOLOGY, vol. 174, no. 17, September 1992 (1992-09-01), pages 5639 - 5646, XP002020553 *
VAN DER MEER, I.M., ET AL .: "FRUCTAN AS A NEW CARBOHYDRATE SINK IN TRANSGENIC POTATO PLANTS", THE PLANT CELL, vol. 6, April 1994 (1994-04-01), pages 561 - 570, XP002020552 *
YAMAMOTO, S., ET AL .: "THE MODE OF SYNTHESIS OF LEVAN BY BACILLUS SUBTILIS LEVANSUCRASE", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 49, no. 2, February 1985 (1985-02-01), pages 343 - 349, XP002020969 *

Also Published As

Publication number	Publication date
WO1997029186A1 (en)	1997-08-14
EP1015562B1 (de)	2007-08-29
AU1674597A (en)	1997-08-28
DK1015562T3 (da)	2008-01-02
ATE371726T1 (de)	2007-09-15
DE69738085D1 (de)	2007-10-11
EP1015562A1 (de)	2000-07-05
DE69738085T2 (de)	2008-09-18
AU732087B2 (en)	2001-04-12
CA2245818C (en)	2011-11-01
CA2245818A1 (en)	1997-08-14

Legal Events

Date	Code	Title	Description
1997-10-01	PD2B	A search report has been drawn up
2005-04-01	TD	Modifications of names of proprietors of patents	Owner name: ADVANTA SEEDS B.V.
2016-02-10	MK	Patent expired because of reaching the maximum lifetime of a patent	Effective date: 20160206

Publication	Publication Date	Title
US7385104B2 (en)	2008-06-10	Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
Ebskamp et al.	1994	Accumulation of fructose polymers in transgenic tobacco
AU691325B2 (en)	1998-05-14	Isoamylase gene from flaviobacterium sp., compositions containing it and methods using it
US6025542A (en)	2000-02-15	Method for obtaining transgenic plants showing a modified fructan pattern
JP4538408B2 (ja)	2010-09-08	高い最終粘度を持つデンプンを合成する植物細胞及び植物
US9365861B2 (en)	2016-06-14	Truncated alternan sucrase coding nucleic acid molecules
US20040107461A1 (en)	2004-06-03	Glucan chain length domains
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HUT75075A (en)	1997-04-28	Transgenic fructan accumulating crops and methods for their production
AU732087B2 (en)	2001-04-12	Modification of polysaccharides
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US6833491B2 (en)	2004-12-21	Modification of polysaccharides
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MXPA99009083A (es)	2000-07-01	Almidones semejantes a los de las plantas y metodo para hacerlos en huespedes
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