NL1010224C2 - Werkwijze voor het detecteren van ATP. - Google Patents

Werkwijze voor het detecteren van ATP. Download PDF

Info

Publication number: NL1010224C2
Authority: NL; Netherlands
Prior art keywords: luciferase; luciferin; atp; light signal; reaction mixture
Prior art date: 1998-09-30

Application number

NL1010224A

Other languages

English (en)

Dutch (nl)

Inventor

Jan Ter Wiel

Harry Van Lune

Original Assignee

Packard Biosciene B V

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-09-30

Filing date

1998-09-30

Publication date

2000-03-31

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19767908&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NL1010224(C2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1998-09-30 Application filed by Packard Biosciene B V filed Critical Packard Biosciene B V

1998-09-30 Priority to NL1010224A priority Critical patent/NL1010224C2/nl

1999-09-30 Priority to DE69924127T priority patent/DE69924127T3/de

1999-09-30 Priority to AT99949447T priority patent/ATE290604T1/de

1999-09-30 Priority to US09/806,165 priority patent/US6503723B1/en

1999-09-30 Priority to DK99949447T priority patent/DK1117825T4/da

1999-09-30 Priority to AU62319/99A priority patent/AU754602B2/en

1999-09-30 Priority to CA2345721A priority patent/CA2345721C/en

1999-09-30 Priority to PCT/NL1999/000606 priority patent/WO2000018953A1/en

1999-09-30 Priority to JP2000572400A priority patent/JP4637357B2/ja

1999-09-30 Priority to IL14200399A priority patent/IL142003A/xx

1999-09-30 Priority to EP99949447A priority patent/EP1117825B2/de

2000-03-31 Publication of NL1010224C2 publication Critical patent/NL1010224C2/nl

2000-03-31 Application granted granted Critical

Links

238000000034 method Methods 0.000 title claims abstract description 28
239000005089 Luciferase Substances 0.000 claims abstract description 47
108060001084 Luciferase Proteins 0.000 claims abstract description 32
239000011541 reaction mixture Substances 0.000 claims abstract description 32
238000006243 chemical reaction Methods 0.000 claims abstract description 27
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims abstract description 25
150000003839 salts Chemical class 0.000 claims abstract description 24
239000000203 mixture Substances 0.000 claims abstract description 22
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims abstract description 20
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims abstract description 20
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims abstract description 20
239000007853 buffer solution Substances 0.000 claims abstract description 4
239000000243 solution Substances 0.000 claims description 36
230000006037 cell lysis Effects 0.000 claims description 13
239000000872 buffer Substances 0.000 claims description 9
239000003599 detergent Substances 0.000 claims description 7
230000002934 lysing effect Effects 0.000 claims description 7
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims description 2
239000003513 alkali Substances 0.000 claims description 2
230000000694 effects Effects 0.000 abstract description 4
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 57
210000004027 cell Anatomy 0.000 description 46
235000002639 sodium chloride Nutrition 0.000 description 21
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
230000007423 decrease Effects 0.000 description 16
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 13
238000010790 dilution Methods 0.000 description 11
239000012895 dilution Substances 0.000 description 11
108091003079 Bovine Serum Albumin Proteins 0.000 description 10
229940098773 bovine serum albumin Drugs 0.000 description 7
239000011780 sodium chloride Substances 0.000 description 7
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
239000007995 HEPES buffer Substances 0.000 description 6
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HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 5
108090000790 Enzymes Proteins 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 5
HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 5
HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 5
238000011534 incubation Methods 0.000 description 5
239000002609 medium Substances 0.000 description 5
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DJHGAFSJWGLOIV-UHFFFAOYSA-K Arsenate3- Chemical compound [O-][As]([O-])([O-])=O DJHGAFSJWGLOIV-UHFFFAOYSA-K 0.000 description 4
RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 4
UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 4
229940000489 arsenate Drugs 0.000 description 4
230000029918 bioluminescence Effects 0.000 description 4
238000005415 bioluminescence Methods 0.000 description 4
RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 4
239000005516 coenzyme A Substances 0.000 description 4
229940093530 coenzyme a Drugs 0.000 description 4
KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 4
229910001425 magnesium ion Inorganic materials 0.000 description 4
239000003381 stabilizer Substances 0.000 description 4
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 4
241000699802 Cricetulus griseus Species 0.000 description 3
241000254158 Lampyridae Species 0.000 description 3
229910021204 NaH2 PO4 Inorganic materials 0.000 description 3
238000013459 approach Methods 0.000 description 3
238000003556 assay Methods 0.000 description 3
230000001419 dependent effect Effects 0.000 description 3
239000012894 fetal calf serum Substances 0.000 description 3
LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
239000003112 inhibitor Substances 0.000 description 3
238000002347 injection Methods 0.000 description 3
239000007924 injection Substances 0.000 description 3
210000001672 ovary Anatomy 0.000 description 3
JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 3
239000000758 substrate Substances 0.000 description 3
UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 2
LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 description 2
229910052784 alkaline earth metal Inorganic materials 0.000 description 2
150000001450 anions Chemical class 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
150000001768 cations Chemical class 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
230000009089 cytolysis Effects 0.000 description 2
230000005764 inhibitory process Effects 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
239000007788 liquid Substances 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
238000005259 measurement Methods 0.000 description 2
230000003647 oxidation Effects 0.000 description 2
238000007254 oxidation reaction Methods 0.000 description 2
229910052760 oxygen Inorganic materials 0.000 description 2
239000001301 oxygen Substances 0.000 description 2
WLJVXDMOQOGPHL-UHFFFAOYSA-N phenylacetic acid Chemical compound OC(=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-UHFFFAOYSA-N 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
HRGUSFBJBOKSML-UHFFFAOYSA-N 3',5'-di-O-methyltricetin Chemical compound COC1=C(O)C(OC)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 HRGUSFBJBOKSML-UHFFFAOYSA-N 0.000 description 1
DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 1
UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
231100000582 ATP assay Toxicity 0.000 description 1
ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
206010008342 Cervix carcinoma Diseases 0.000 description 1
FCKYPQBAHLOOJQ-UHFFFAOYSA-N Cyclohexane-1,2-diaminetetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)C1CCCCC1N(CC(O)=O)CC(O)=O FCKYPQBAHLOOJQ-UHFFFAOYSA-N 0.000 description 1
OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 description 1
239000007996 HEPPS buffer Substances 0.000 description 1
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102000004407 Lactalbumin Human genes 0.000 description 1
239000007993 MOPS buffer Substances 0.000 description 1
108010058846 Ovalbumin Proteins 0.000 description 1
241000254064 Photinus pyralis Species 0.000 description 1
IDDMFNIRSJVBHE-UHFFFAOYSA-N Piscigenin Natural products COC1=C(O)C(OC)=CC(C=2C(C3=C(O)C=C(O)C=C3OC=2)=O)=C1 IDDMFNIRSJVBHE-UHFFFAOYSA-N 0.000 description 1
229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
241001340896 Pyralis Species 0.000 description 1
239000012979 RPMI medium Substances 0.000 description 1
108010071390 Serum Albumin Proteins 0.000 description 1
102000007562 Serum Albumin Human genes 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
230000001464 adherent effect Effects 0.000 description 1
239000002671 adjuvant Substances 0.000 description 1
-1 alkaline earth metal salts Chemical class 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
239000012298 atmosphere Substances 0.000 description 1
239000002585 base Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000011575 calcium Substances 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
208000019065 cervical carcinoma Diseases 0.000 description 1
239000002738 chelating agent Substances 0.000 description 1
210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
238000001514 detection method Methods 0.000 description 1
KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
235000011180 diphosphates Nutrition 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
229910000397 disodium phosphate Inorganic materials 0.000 description 1
235000019800 disodium phosphate Nutrition 0.000 description 1
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
230000005281 excited state Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
230000005283 ground state Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
239000000644 isotonic solution Substances 0.000 description 1
239000006166 lysate Substances 0.000 description 1
159000000003 magnesium salts Chemical class 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
235000019799 monosodium phosphate Nutrition 0.000 description 1
230000006959 non-competitive inhibition Effects 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
229940092253 ovalbumin Drugs 0.000 description 1
239000008188 pellet Substances 0.000 description 1
239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
229960003424 phenylacetic acid Drugs 0.000 description 1
239000003279 phenylacetic acid Substances 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
102000040430 polynucleotide Human genes 0.000 description 1
108091033319 polynucleotide Proteins 0.000 description 1
239000002157 polynucleotide Substances 0.000 description 1
210000004765 promyelocyte Anatomy 0.000 description 1
239000002516 radical scavenger Substances 0.000 description 1
239000003352 sequestering agent Substances 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000000725 suspension Substances 0.000 description 1
FBWNMEQMRUMQSO-UHFFFAOYSA-N tergitol NP-9 Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCO)C=C1 FBWNMEQMRUMQSO-UHFFFAOYSA-N 0.000 description 1
238000012360 testing method Methods 0.000 description 1
BMCJATLPEJCACU-UHFFFAOYSA-N tricin Natural products COc1cc(OC)c(O)c(c1)C2=CC(=O)c3c(O)cc(O)cc3O2 BMCJATLPEJCACU-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

NL1010224A 1998-09-30 1998-09-30 Werkwijze voor het detecteren van ATP. NL1010224C2 (nl)

Priority Applications (11)

Application Number	Priority Date	Filing Date	Title
NL1010224A NL1010224C2 (nl)	1998-09-30	1998-09-30	Werkwijze voor het detecteren van ATP.
DK99949447T DK1117825T4 (da)	1998-09-30	1999-09-30	Fremgangsmåde til detektion af ATP
AT99949447T ATE290604T1 (de)	1998-09-30	1999-09-30	Methode zum nachweis von atp
US09/806,165 US6503723B1 (en)	1998-09-30	1999-09-30	Method for detecting ATP
DE69924127T DE69924127T3 (de)	1998-09-30	1999-09-30	Methode zum nachweis von atp
AU62319/99A AU754602B2 (en)	1998-09-30	1999-09-30	Method for detecting ATP
CA2345721A CA2345721C (en)	1998-09-30	1999-09-30	High salt concentration to prolong light signal in luciferase reactions for atp measurement
PCT/NL1999/000606 WO2000018953A1 (en)	1998-09-30	1999-09-30	Method for detecting atp
JP2000572400A JP4637357B2 (ja)	1998-09-30	1999-09-30	Ａｔｐを検出する方法
IL14200399A IL142003A (en)	1998-09-30	1999-09-30	Method for detecting atp
EP99949447A EP1117825B2 (de)	1998-09-30	1999-09-30	Methode zum nachweis von atp

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
NL1010224A NL1010224C2 (nl)	1998-09-30	1998-09-30	Werkwijze voor het detecteren van ATP.
NL1010224		1998-09-30

Publications (1)

Publication Number	Publication Date
NL1010224C2 true NL1010224C2 (nl)	2000-03-31

Family

ID=19767908

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL1010224A NL1010224C2 (nl)	1998-09-30	1998-09-30	Werkwijze voor het detecteren van ATP.

Country Status (10)

Country	Link
US (1)	US6503723B1 (de)
EP (1)	EP1117825B2 (de)
JP (1)	JP4637357B2 (de)
AT (1)	ATE290604T1 (de)
AU (1)	AU754602B2 (de)
CA (1)	CA2345721C (de)
DE (1)	DE69924127T3 (de)
IL (1)	IL142003A (de)
NL (1)	NL1010224C2 (de)
WO (1)	WO2000018953A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB0030727D0 (en) *	2000-12-15	2001-01-31	Lumitech Uk Ltd	Methods and kits for detecting kinase activity
US7083911B2 (en) *	2001-02-16	2006-08-01	Promega Corporation	Method for detection of ATP
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Publication number	Publication date
ATE290604T1 (de)	2005-03-15
JP2002525123A (ja)	2002-08-13
DE69924127T3 (de)	2010-01-14
AU6231999A (en)	2000-04-17
DE69924127T2 (de)	2006-04-06
EP1117825B1 (de)	2005-03-09
AU754602B2 (en)	2002-11-21
US6503723B1 (en)	2003-01-07
CA2345721C (en)	2011-01-11
IL142003A0 (en)	2002-03-10
EP1117825B2 (de)	2009-06-24
CA2345721A1 (en)	2000-04-06
WO2000018953A1 (en)	2000-04-06
EP1117825A1 (de)	2001-07-25
IL142003A (en)	2005-08-31
JP4637357B2 (ja)	2011-02-23
DE69924127D1 (de)	2005-04-14

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2018-10-03	MK	Patent expired because of reaching the maximum lifetime of a patent	Effective date: 20180929

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