NL8600286A - Nieuwe thermostabiele zure alfa-amylase en werkwijze voor de bereiding ervan. - Google Patents

Nieuwe thermostabiele zure alfa-amylase en werkwijze voor de bereiding ervan. Download PDF

Info

Publication number: NL8600286A
Authority: NL; Netherlands
Prior art keywords: alpha; amylase; enzyme; starch; solution
Prior art date: 1985-02-08

Application number

NL8600286A

Other languages

English (en)

Dutch (nl)

Original Assignee

Cpc International Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-02-08

Filing date

1986-02-06

Publication date

1986-09-01

1986-02-06 Application filed by Cpc International Inc filed Critical Cpc International Inc

1986-09-01 Publication of NL8600286A publication Critical patent/NL8600286A/nl

Links

238000000034 method Methods 0.000 title claims description 19
239000004382 Amylase Substances 0.000 title description 9
102000013142 Amylases Human genes 0.000 title description 9
108010065511 Amylases Proteins 0.000 title description 9
235000019418 amylase Nutrition 0.000 title description 8
239000002253 acid Substances 0.000 title 1
102000004139 alpha-Amylases Human genes 0.000 claims description 62
108090000637 alpha-Amylases Proteins 0.000 claims description 62
102000004190 Enzymes Human genes 0.000 claims description 39
108090000790 Enzymes Proteins 0.000 claims description 39
229940088598 enzyme Drugs 0.000 claims description 39
229940024171 alpha-amylase Drugs 0.000 claims description 37
229920002472 Starch Polymers 0.000 claims description 35
239000008107 starch Substances 0.000 claims description 35
235000019698 starch Nutrition 0.000 claims description 35
239000000243 solution Substances 0.000 claims description 27
230000000694 effects Effects 0.000 claims description 21
241000193447 Thermoanaerobacter thermohydrosulfuricus Species 0.000 claims description 9
238000002360 preparation method Methods 0.000 claims description 8
238000006460 hydrolysis reaction Methods 0.000 claims description 6
230000007062 hydrolysis Effects 0.000 claims description 5
230000003301 hydrolyzing effect Effects 0.000 claims description 4
238000002264 polyacrylamide gel electrophoresis Methods 0.000 claims description 4
239000007864 aqueous solution Substances 0.000 claims description 2
239000007900 aqueous suspension Substances 0.000 claims description 2
235000015097 nutrients Nutrition 0.000 claims description 2
239000002609 medium Substances 0.000 description 10
238000000855 fermentation Methods 0.000 description 8
244000005700 microbiome Species 0.000 description 8
238000006243 chemical reaction Methods 0.000 description 7
239000000203 mixture Substances 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
230000004151 fermentation Effects 0.000 description 6
238000004519 manufacturing process Methods 0.000 description 6
241000194108 Bacillus licheniformis Species 0.000 description 5
239000011575 calcium Substances 0.000 description 5
238000012360 testing method Methods 0.000 description 5
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
241001509321 Clostridium thermoamylolyticum Species 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
239000000706 filtrate Substances 0.000 description 4
239000001963 growth medium Substances 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
229940088594 vitamin Drugs 0.000 description 4
235000013343 vitamin Nutrition 0.000 description 4
239000011782 vitamin Substances 0.000 description 4
229930003231 vitamin Natural products 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
241000193385 Geobacillus stearothermophilus Species 0.000 description 3
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
229940041514 candida albicans extract Drugs 0.000 description 3
239000004615 ingredient Substances 0.000 description 3
229910052500 inorganic mineral Inorganic materials 0.000 description 3
239000011707 mineral Substances 0.000 description 3
235000018102 proteins Nutrition 0.000 description 3
238000000746 purification Methods 0.000 description 3
239000007974 sodium acetate buffer Substances 0.000 description 3
238000003756 stirring Methods 0.000 description 3
150000003722 vitamin derivatives Chemical class 0.000 description 3
239000012138 yeast extract Substances 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
241000193403 Clostridium Species 0.000 description 2
102100022624 Glucoamylase Human genes 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
238000010521 absorption reaction Methods 0.000 description 2
239000008351 acetate buffer Substances 0.000 description 2
230000002378 acidificating effect Effects 0.000 description 2
239000000908 ammonium hydroxide Substances 0.000 description 2
239000000872 buffer Substances 0.000 description 2
239000007853 buffer solution Substances 0.000 description 2
238000001914 filtration Methods 0.000 description 2
238000001502 gel electrophoresis Methods 0.000 description 2
239000008103 glucose Substances 0.000 description 2
238000002955 isolation Methods 0.000 description 2
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
239000002244 precipitate Substances 0.000 description 2
LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
239000011734 sodium Substances 0.000 description 2
229910052708 sodium Inorganic materials 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
AGBQKNBQESQNJD-SSDOTTSWSA-N (R)-lipoic acid Chemical compound OC(=O)CCCC[C@@H]1CCSS1 AGBQKNBQESQNJD-SSDOTTSWSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
VVIAGPKUTFNRDU-UHFFFAOYSA-N 6S-folinic acid Natural products C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-UHFFFAOYSA-N 0.000 description 1
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
244000063299 Bacillus subtilis Species 0.000 description 1
235000014469 Bacillus subtilis Nutrition 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
238000009631 Broth culture Methods 0.000 description 1
239000004215 Carbon black (E152) Substances 0.000 description 1
GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
241000193401 Clostridium acetobutylicum Species 0.000 description 1
241000193171 Clostridium butyricum Species 0.000 description 1
229920002261 Corn starch Polymers 0.000 description 1
229920000742 Cotton Polymers 0.000 description 1
AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
229920002245 Dextrose equivalent Polymers 0.000 description 1
RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
MPJKWIXIYCLVCU-UHFFFAOYSA-N Folinic acid Natural products NC1=NC2=C(N(C=O)C(CNc3ccc(cc3)C(=O)NC(CCC(=O)O)CC(=O)O)CN2)C(=O)N1 MPJKWIXIYCLVCU-UHFFFAOYSA-N 0.000 description 1
230000005526 G1 to G0 transition Effects 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
239000007836 KH2PO4 Substances 0.000 description 1
229920002774 Maltodextrin Polymers 0.000 description 1
239000005913 Maltodextrin Substances 0.000 description 1
241001494479 Pecora Species 0.000 description 1
PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
241000194017 Streptococcus Species 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
239000008272 agar Substances 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
AGBQKNBQESQNJD-UHFFFAOYSA-N alpha-Lipoic acid Natural products OC(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-N 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
229960004050 aminobenzoic acid Drugs 0.000 description 1
235000019270 ammonium chloride Nutrition 0.000 description 1
229940025131 amylases Drugs 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000008120 corn starch Substances 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
229910000397 disodium phosphate Inorganic materials 0.000 description 1
235000019800 disodium phosphate Nutrition 0.000 description 1
230000007071 enzymatic hydrolysis Effects 0.000 description 1
238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
VVIAGPKUTFNRDU-ABLWVSNPSA-N folinic acid Chemical compound C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-ABLWVSNPSA-N 0.000 description 1
235000008191 folinic acid Nutrition 0.000 description 1
239000011672 folinic acid Substances 0.000 description 1
230000008014 freezing Effects 0.000 description 1
238000007710 freezing Methods 0.000 description 1
239000012869 germination medium Substances 0.000 description 1
239000011491 glass wool Substances 0.000 description 1
239000012510 hollow fiber Substances 0.000 description 1
229930195733 hydrocarbon Natural products 0.000 description 1
150000002430 hydrocarbons Chemical class 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
239000011630 iodine Substances 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
239000004310 lactic acid Substances 0.000 description 1
235000014655 lactic acid Nutrition 0.000 description 1
229960001691 leucovorin Drugs 0.000 description 1
238000012417 linear regression Methods 0.000 description 1
235000019136 lipoic acid Nutrition 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
229940035034 maltodextrin Drugs 0.000 description 1
229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
239000000463 material Substances 0.000 description 1
235000012054 meals Nutrition 0.000 description 1
238000011177 media preparation Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
238000013508 migration Methods 0.000 description 1
230000005012 migration Effects 0.000 description 1
238000002156 mixing Methods 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
229960003512 nicotinic acid Drugs 0.000 description 1
235000001968 nicotinic acid Nutrition 0.000 description 1
239000011664 nicotinic acid Substances 0.000 description 1
MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
229940055726 pantothenic acid Drugs 0.000 description 1
235000019161 pantothenic acid Nutrition 0.000 description 1
239000011713 pantothenic acid Substances 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
238000012545 processing Methods 0.000 description 1
239000000047 product Substances 0.000 description 1
239000012521 purified sample Substances 0.000 description 1
235000008160 pyridoxine Nutrition 0.000 description 1
239000011677 pyridoxine Substances 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
229960002477 riboflavin Drugs 0.000 description 1
235000019192 riboflavin Nutrition 0.000 description 1
239000002151 riboflavin Substances 0.000 description 1
210000004767 rumen Anatomy 0.000 description 1
239000000523 sample Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
229910052979 sodium sulfide Inorganic materials 0.000 description 1
GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
241000894007 species Species 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
235000020357 syrup Nutrition 0.000 description 1
239000006188 syrup Substances 0.000 description 1
238000010998 test method Methods 0.000 description 1
235000019157 thiamine Nutrition 0.000 description 1
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
229960003495 thiamine Drugs 0.000 description 1
239000011721 thiamine Substances 0.000 description 1
229960002663 thioctic acid Drugs 0.000 description 1
230000001960 triggered effect Effects 0.000 description 1
238000003828 vacuum filtration Methods 0.000 description 1
229940011671 vitamin b6 Drugs 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/842—Clostridium

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

NL8600286A 1985-02-08 1986-02-06 Nieuwe thermostabiele zure alfa-amylase en werkwijze voor de bereiding ervan. NL8600286A (nl)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US69977285		1985-02-08
US06/699,772 US4613570A (en)	1985-02-08	1985-02-08	Novel thermostable, aciduric alpha-amylase and method for its production

Publications (1)

Publication Number	Publication Date
NL8600286A true NL8600286A (nl)	1986-09-01

Family

ID=24810850

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL8600286A NL8600286A (nl)	1985-02-08	1986-02-06	Nieuwe thermostabiele zure alfa-amylase en werkwijze voor de bereiding ervan.

Country Status (7)

Country	Link
US (1)	US4613570A (da)
JP (1)	JPS61185185A (da)
DE (1)	DE3603348A1 (da)
DK (1)	DK60386A (da)
FR (1)	FR2577237B1 (da)
MX (2)	MX163267B (da)
NL (1)	NL8600286A (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS61115496A (ja) *	1984-11-09	1986-06-03	Hitachi Ltd	殿粉の液化方法
DE3582020D1 (de) *	1984-11-09	1991-04-11	Hitachi Ltd	Thermostabile alpha-amylase produzierende thermostabile anaerobe bakterie, thermostabile alpha-amylase und verfahren zur deren herstellung.
FI81112C (fi) *	1986-08-27	1990-09-10	Alko Ab Oy	Amylas av ny typ.
DE3639267A1 (de) *	1986-11-17	1988-09-22	Antranikian Garabed	Verfahren zur foerderung der exkretion von amylolytischen enzymen aus bakterien sowie nach dem verfahren erhaltene enzyme
ATE124719T1 (de) *	1987-07-17	1995-07-15	Modrovich Ivan Endre	Stabiles alpha-amylase-reagenz.
US5188956A (en) *	1988-07-01	1993-02-23	Showa Denka K.K.	Thermostable amylase
US5010008A (en) *	1988-12-12	1991-04-23	Enzyme Bio-Systems Ltd.	Stable liquid enzyme concentrate and process for its production
GB201716845D0 (en)	2017-10-13	2017-11-29	Green Biologics Ltd	Process
CN108823185A (zh) *	2018-06-25	2018-11-16	安徽新熙盟生物科技有限公司	高酶活发酵液的培养方法及提取耐酸α-淀粉酶的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4284722A (en) *	1978-08-16	1981-08-18	Cpc International Inc.	Heat and acid-stable alpha-amylase enzymes and processes for producing the same
US4578352A (en) *	1983-07-13	1986-03-25	Cpc International Inc.	Novel thermostable, aciduric alpha-amylase and method for its production
US4536477A (en) *	1983-08-17	1985-08-20	Cpc International Inc.	Thermostable glucoamylase and method for its production
ES8602115A1 (es) *	1983-10-11	1985-11-01	Espanola Petrol	Procedimiento para producir -amilasas termoestables por cultivo de microorganismos a altas temperaturas
DE3582020D1 (de) *	1984-11-09	1991-04-11	Hitachi Ltd	Thermostabile alpha-amylase produzierende thermostabile anaerobe bakterie, thermostabile alpha-amylase und verfahren zur deren herstellung.

1985
- 1985-02-08 US US06/699,772 patent/US4613570A/en not_active Expired - Fee Related
1986
- 1986-02-04 DE DE19863603348 patent/DE3603348A1/de not_active Withdrawn
- 1986-02-06 NL NL8600286A patent/NL8600286A/nl not_active Application Discontinuation
- 1986-02-07 MX MX1487A patent/MX163267B/es unknown
- 1986-02-07 JP JP61024270A patent/JPS61185185A/ja active Pending
- 1986-02-07 MX MX025687A patent/MX173840B/es unknown
- 1986-02-07 FR FR868601699A patent/FR2577237B1/fr not_active Expired
- 1986-02-07 DK DK60386A patent/DK60386A/da not_active Application Discontinuation

Also Published As

Publication number	Publication date
US4613570A (en)	1986-09-23
DK60386D0 (da)	1986-02-07
JPS61185185A (ja)	1986-08-18
DE3603348A1 (de)	1986-08-28
FR2577237A1 (fr)	1986-08-14
MX173840B (es)	1994-03-30
DK60386A (da)	1986-08-09
FR2577237B1 (fr)	1989-12-22
MX163267B (es)	1992-03-31

Legal Events

Date	Code	Title	Description
1990-10-01	BV	The patent application has lapsed

Publication	Publication Date	Title
US4536477A (en)	1985-08-20	Thermostable glucoamylase and method for its production
Aguilar et al.	2000	Purification and characterization of an extracellular α-amylase produced by Lactobacillus manihotivorans LMG 18010T, an amylolytic lactic acid bacterium
Vishnu et al.	2000	Direct conversion of starch to L (+) lactic acid by amylase producing Lactobacillus amylophilus GV6
KR830010192A (ko)	1983-12-26	디브랜칭 효소 생성물, 그 제법 및 그 이용 방법
US4628028A (en)	1986-12-09	Novel thermostable pullulanase enzyme and method for its production
US4578352A (en)	1986-03-25	Novel thermostable, aciduric alpha-amylase and method for its production
Hulcher et al.	1958	Disaccharide preference of an aerobic cellulolytic bacterium, Cellvibrio gilvus n. sp
NL8600286A (nl)	1986-09-01	Nieuwe thermostabiele zure alfa-amylase en werkwijze voor de bereiding ervan.
US4734365A (en)	1988-03-29	Process for liquefying starch
US4647538A (en)	1987-03-03	Thermostable beta-amylase
Hyun et al.	1985	Regulation and genetic enhancement of beta-amylase production in Clostridium thermosulfurogenes
Lindgren et al.	1984	Amylolytic lactic acid bacteria in fish silage
De Cort et al.	1994	Localization and characterization of α-glucosidase activity in Lactobacillus brevis
Lin et al.	1994	General characteristics of thermostable amylopullulanases and amylases from the alkalophilic Bacillus sp. TS-23
Kim et al.	1993	Pullulanases of alkaline and broad pH range from a newly isolated alkalophilic Bacillus sp. S-1 and a Micrococcus sp. Y-1
US4737459A (en)	1988-04-12	Regulation and enhancement of enzyme production
US4929557A (en)	1990-05-29	Thermostable amylases and pullulanases from two anaerobic microorganisms
CA1081633A (en)	1980-07-15	Heat and acid-stable alpha-amylase enzymes and processes for producing the same
de Souza Teodoro et al.	2000	Culture conditions for the production of thermostable amylase by Bacillus sp
Kademi et al.	1999	Effect of culture conditions on growth and esterase production by the moderate thermophile Bacillus circulans MAS2
US4925795A (en)	1990-05-15	Method of using G-4 amylase to produce high maltotetraose and high maltose content starch hydrolysates
Raj et al.	2009	Study of starch degrading bacteria from kitchen waste soil in the production of amylase by using paddy straw
Emanuilova et al.	1992	Effect of carbon source and dissolved oxygen level on cell growth and pullulanase production by Bacillus stearothermophilus G-82
JP2866460B2 (ja)	1999-03-08	多糖類の糖化方法
JPS6331195B2 (da)	1988-06-22