NO20053385L - IL-21-produksjon i prokaryote verter. - Google Patents

IL-21-produksjon i prokaryote verter.

Info

Publication number
NO20053385L
NO20053385L NO20053385A NO20053385A NO20053385L NO 20053385 L NO20053385 L NO 20053385L NO 20053385 A NO20053385 A NO 20053385A NO 20053385 A NO20053385 A NO 20053385A NO 20053385 L NO20053385 L NO 20053385L
Authority
NO
Norway
Prior art keywords
coli
production
expression
prokaryotic hosts
vectors
Prior art date
Application number
NO20053385A
Other languages
English (en)
Other versions
NO334561B1 (no
Inventor
Susan D Holderman
Chung Chan
Bruce L Zamost
Douglas C Covert
Hong Y Liu
Karen S De Jongh
Jeffrey D Meyer
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of NO20053385L publication Critical patent/NO20053385L/no
Publication of NO334561B1 publication Critical patent/NO334561B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Ekspresjonsvektorene og fremgangsmåtene som benytter et E.coli-uttrykkingssystem for storskalaproduksjon av IL-21 er beskrevet. Vektorene benytter den IL-21-kodende sekvensen med spesifikke endringer i nukleotider for å optimalisere kodons og mRNA-sekundærstruktur for translatering i E.coli. Ved å benytte ekspresjonsvektorene ble IL-21-genet produsert i E.coli til et nivå på større enn I g/I i tilsatt ladningsfermentering. Også inkludert er OmpTmanglende E.coli-stamme fransformert med en IL-21 -ekspresj onsvektor.
NO20053385A 2002-12-13 2005-07-12 Ekspresjonsvektor for fremstilling av IL-21-protein, prokaryot vertcelle for IL-21-produksjon og fremgangsmåte for fremstilling av IL-21-proteiner. NO334561B1 (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43344802P 2002-12-13 2002-12-13
US43345202P 2002-12-13 2002-12-13
PCT/US2003/039764 WO2004055168A2 (en) 2002-12-13 2003-12-12 Il-21 production in prokaryotic hosts

Publications (2)

Publication Number Publication Date
NO20053385L true NO20053385L (no) 2005-07-12
NO334561B1 NO334561B1 (no) 2014-04-07

Family

ID=32600150

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20053385A NO334561B1 (no) 2002-12-13 2005-07-12 Ekspresjonsvektor for fremstilling av IL-21-protein, prokaryot vertcelle for IL-21-produksjon og fremgangsmåte for fremstilling av IL-21-proteiner.

Country Status (17)

Country Link
US (4) US7250274B2 (no)
EP (1) EP1573006B1 (no)
JP (1) JP4664684B2 (no)
KR (1) KR101098897B1 (no)
AT (1) ATE448298T1 (no)
AU (1) AU2003302250B2 (no)
BR (1) BR0317243A (no)
CA (1) CA2507817C (no)
DE (1) DE60330044D1 (no)
DK (1) DK1573006T3 (no)
ES (1) ES2334127T3 (no)
IL (2) IL168697A (no)
MX (1) MXPA05006117A (no)
NO (1) NO334561B1 (no)
PL (1) PL209009B1 (no)
RU (1) RU2354703C2 (no)
WO (1) WO2004055168A2 (no)

Families Citing this family (33)

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US6307024B1 (en) 1999-03-09 2001-10-23 Zymogenetics, Inc. Cytokine zalpha11 Ligand
WO2004112703A2 (en) * 2003-06-19 2004-12-29 Centocor, Inc. Interleukin-21 analogs
DE602004029173D1 (de) * 2003-10-10 2010-10-28 Novo Nordisk As Il-21-derivate
US20060228331A1 (en) * 2003-10-10 2006-10-12 Novo Nordisk A/S IL-21 Derivatives and variants
EP2633866A3 (en) * 2003-10-17 2013-12-18 Novo Nordisk A/S Combination therapy
BRPI0511187A (pt) * 2004-05-20 2007-12-04 Zymogenetics Inc método para tratar cáncer em um indivìduo
AU2006237329B2 (en) 2005-04-18 2012-04-12 Novo Nordisk A/S IL-21 variants
EP1885847B1 (en) * 2005-05-26 2011-07-06 Cytos Biotechnology AG Scalable fermentation process
CA2611200A1 (en) * 2005-06-06 2006-12-14 Novo Nordisk A/S Stabilised il-21 compositions
WO2007025987A1 (en) 2005-08-30 2007-03-08 Novo Nordisk A/S Expression of proteins in e.coli
WO2007039147A1 (en) 2005-09-22 2007-04-12 Inserm (Institut National De La Sante Et De La Recherche Medicale) Methods of using il-21
WO2007041713A1 (en) * 2005-10-04 2007-04-12 Zymogenetics, Inc. Production and purification of il-29
AU2006340750B2 (en) 2005-11-28 2013-03-07 Zymogenetics, Inc. IL-21 antagonists
WO2007071768A1 (en) * 2005-12-23 2007-06-28 Novo Nordisk A/S Purification of proteins using preparative reverse phase chromatography (rpc)
CA2657135C (en) 2006-07-27 2016-03-15 Wyeth High-cell density fed-batch fermentation process for producing recombinant protein
AU2007310777A1 (en) 2006-10-26 2008-05-02 Novo Nordisk A/S IL-21 variants
CA2671665A1 (en) * 2006-12-21 2008-06-26 Novo-Nordisk A/S Interleukin-21 variants with altered binding to the il-21 receptor
EP2118121A1 (en) * 2007-03-01 2009-11-18 Novo Nordisk A/S Expression of proteins in e. coli
JP5358840B2 (ja) * 2007-11-24 2013-12-04 独立行政法人産業技術総合研究所 Ｇｆｐ（緑色蛍光蛋白質）の機能賦活・回復方法
EP3103813A1 (en) 2007-12-07 2016-12-14 ZymoGenetics, Inc. Anti-human il-21 monoclonal antibodies
US9927440B2 (en) * 2009-11-25 2018-03-27 Duke University Protein engineering
WO2011149917A1 (en) * 2010-05-25 2011-12-01 Adlyfe, Inc. STABILIZED AMYLOID- β OLIGOMERS AND USES THEREOF
WO2013169693A1 (en) 2012-05-09 2013-11-14 Bristol-Myers Squibb Company Methods of treating cancer using an il-21 polypeptide and an anti-pd-1 antibody
JP6086378B2 (ja) * 2012-11-30 2017-03-01 国立大学法人お茶の水女子大学 組み換えヒト膵臓リパーゼの調製方法
EP3147369B1 (en) * 2014-05-21 2025-08-06 Ajinomoto Co., Inc. Fibroin-like protein production method
RU2620073C2 (ru) * 2015-06-02 2017-05-22 Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России Оптимизированный ген, кодирующий рекомбинантный белок - аналог интерферона альфа-17 человека
RU2614264C2 (ru) * 2015-06-19 2017-03-24 Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России Оптимизированный ген, кодирующий рекомбинантный белок - аналог интерферона бета человека
RU2614124C9 (ru) * 2015-10-01 2020-04-22 Федеральное государственное бюджетное учреждение "Государственный научный центр "Институт иммунологии" Федерального медико-биологического агентства России (ФГБУ "ГНЦ Институт иммунологии" ФМБА России) Оптимизированный ген, кодирующий рекомбинантный белок ипфiii
JP2017110001A (ja) * 2015-12-16 2017-06-22 株式会社 資生堂 タブレット型凍結乾燥化粧料
JP6404405B1 (ja) * 2017-06-14 2018-10-10 株式会社 資生堂 タブレット型凍結乾燥化粧料
WO2018235147A1 (ja) * 2017-06-19 2018-12-27 株式会社 資生堂 タブレット型凍結乾燥化粧料
CA3157881A1 (en) * 2019-10-14 2021-04-22 Inbiose N.V. Production of bioproduct in a host cell
AU2021286574A1 (en) * 2020-06-09 2023-01-19 Icosavax, Inc. Method of making virus-like particle

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4344697A (en) * 1996-10-04 1998-04-24 Amgen, Inc. Pharmaceutical compositions containing an mpl ligand
US6057128A (en) * 1998-03-17 2000-05-02 Genetics Institute, Inc. MU-1, member of the cytokine receptor family
US6307024B1 (en) * 1999-03-09 2001-10-23 Zymogenetics, Inc. Cytokine zalpha11 Ligand
EP1881070B1 (en) * 1999-03-09 2012-10-03 Zymogenetics, Inc. Human cytokine as ligand of the zalpha receptor and uses thereof
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
CA2481304A1 (en) 2002-03-27 2003-10-09 Patrick Hwu Method for treating cancer in humans
WO2003087320A2 (en) 2002-04-09 2003-10-23 Beth Israel Deaconess Medical Center, Inc. Antagonists of il-21 and modulation of il-21-mediated t cell responses
PL376118A1 (en) 2002-10-11 2005-12-12 Novo Nordisk A/S Treatment of allergic conditions by use of il 21
CN1513993A (zh) 2002-12-31 2004-07-21 北京博泰迪生物工程科技开发有限公司 中国人基因组cDNA文库白细胞介素21的编码基因序列及其蛋白质的氨基酸序列
WO2004112703A2 (en) * 2003-06-19 2004-12-29 Centocor, Inc. Interleukin-21 analogs
JP2007512008A (ja) 2003-11-24 2007-05-17 ノボ　ノルディスク　アクティーゼルスカブ 融合タンパク質及び該タンパク質の切断方法

Also Published As

Publication number Publication date
JP2006509525A (ja) 2006-03-23
CA2507817A1 (en) 2004-07-01
RU2005117338A (ru) 2006-03-20
US20070048842A1 (en) 2007-03-01
EP1573006A4 (en) 2006-06-14
KR20050090393A (ko) 2005-09-13
KR101098897B1 (ko) 2011-12-26
DK1573006T3 (da) 2010-03-22
ES2334127T3 (es) 2010-03-05
EP1573006B1 (en) 2009-11-11
US20060134754A1 (en) 2006-06-22
DE60330044D1 (de) 2009-12-24
US7745176B2 (en) 2010-06-29
EP1573006A2 (en) 2005-09-14
WO2004055168A2 (en) 2004-07-01
AU2003302250B2 (en) 2009-08-27
US7767199B2 (en) 2010-08-03
IL168697A (en) 2011-10-31
US20070048798A1 (en) 2007-03-01
MXPA05006117A (es) 2005-09-30
CA2507817C (en) 2014-04-22
JP4664684B2 (ja) 2011-04-06
US20070048263A1 (en) 2007-03-01
PL377291A1 (pl) 2006-01-23
ATE448298T1 (de) 2009-11-15
BR0317243A (pt) 2005-11-01
NO334561B1 (no) 2014-04-07
IL198074A0 (en) 2011-08-01
US7250274B2 (en) 2007-07-31
WO2004055168A3 (en) 2005-06-02
AU2003302250A1 (en) 2004-07-09
RU2354703C2 (ru) 2009-05-10
US7622561B2 (en) 2009-11-24
PL209009B1 (pl) 2011-07-29

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