NO313593B1 - Fremgangsmåte for utvelgelse av rekombinante mikroorganismer hvis overflate omfatter i det minste et molekyl med enzymatiskaktivitet - Google Patents

Fremgangsmåte for utvelgelse av rekombinante mikroorganismer hvis overflate omfatter i det minste et molekyl med enzymatiskaktivitet Download PDF

Info

Publication number: NO313593B1
Authority: NO; Norway
Prior art keywords: accordance; molecule; enzymatic activity; solid substrate; selection
Prior art date: 1991-11-29

Application number

NO19941731A

Other languages

English (en)

Norwegian (no)

Other versions

NO941731L (no

NO941731D0 (no

Inventor

Fastrez Jacques

Original Assignee

Univ Catholique Louvain

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-11-29

Filing date

1994-05-10

Publication date

2002-10-28

1994-05-10 Application filed by Univ Catholique Louvain filed Critical Univ Catholique Louvain

1994-05-10 Publication of NO941731D0 publication Critical patent/NO941731D0/no

1994-06-21 Publication of NO941731L publication Critical patent/NO941731L/no

2002-10-28 Publication of NO313593B1 publication Critical patent/NO313593B1/no

Links

238000000034 method Methods 0.000 title claims abstract description 67
244000005700 microbiome Species 0.000 title claims abstract description 52
230000002255 enzymatic effect Effects 0.000 title claims abstract description 40
239000007787 solid Substances 0.000 claims abstract description 45
239000000758 substrate Substances 0.000 claims abstract description 41
229940125808 covalent inhibitor Drugs 0.000 claims abstract description 26
239000003550 marker Substances 0.000 claims description 37
102000004190 Enzymes Human genes 0.000 claims description 30
108090000790 Enzymes Proteins 0.000 claims description 30
108090000765 processed proteins & peptides Proteins 0.000 claims description 24
239000003446 ligand Substances 0.000 claims description 20
239000000203 mixture Substances 0.000 claims description 19
108010090804 Streptavidin Proteins 0.000 claims description 11
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
238000003776 cleavage reaction Methods 0.000 claims description 9
102000004196 processed proteins & peptides Human genes 0.000 claims description 9
230000007017 scission Effects 0.000 claims description 9
230000003197 catalytic effect Effects 0.000 claims description 8
150000002148 esters Chemical class 0.000 claims description 8
108090001008 Avidin Proteins 0.000 claims description 6
229960002685 biotin Drugs 0.000 claims description 5
235000020958 biotin Nutrition 0.000 claims description 5
239000011616 biotin Substances 0.000 claims description 5
125000000524 functional group Chemical group 0.000 claims description 5
241000724791 Filamentous phage Species 0.000 claims description 4
238000004519 manufacturing process Methods 0.000 claims description 4
DEQPBRIACBATHE-FXQIFTODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-2-iminopentanoic acid Chemical compound N1C(=O)N[C@@H]2[C@H](CCCC(=N)C(=O)O)SC[C@@H]21 DEQPBRIACBATHE-FXQIFTODSA-N 0.000 claims description 3
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims description 3
125000002228 disulfide group Chemical group 0.000 claims description 3
238000006911 enzymatic reaction Methods 0.000 claims description 3
241000238876 Acari Species 0.000 claims description 2
AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims description 2
108091005804 Peptidases Proteins 0.000 claims description 2
239000004365 Protease Substances 0.000 claims description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
230000002378 acidificating effect Effects 0.000 claims description 2
150000002009 diols Chemical group 0.000 claims description 2
230000007704 transition Effects 0.000 claims description 2
241000700605 Viruses Species 0.000 claims 1
239000013038 irreversible inhibitor Substances 0.000 claims 1
108090000623 proteins and genes Proteins 0.000 description 57
102000004169 proteins and genes Human genes 0.000 description 33
235000018102 proteins Nutrition 0.000 description 31
229940088598 enzyme Drugs 0.000 description 30
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 21
239000003112 inhibitor Substances 0.000 description 19
239000000047 product Substances 0.000 description 19
239000000243 solution Substances 0.000 description 18
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 16
239000002609 medium Substances 0.000 description 15
230000000694 effects Effects 0.000 description 14
YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 12
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
239000000872 buffer Substances 0.000 description 12
238000001514 detection method Methods 0.000 description 11
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
230000015572 biosynthetic process Effects 0.000 description 10
238000010828 elution Methods 0.000 description 10
238000002474 experimental method Methods 0.000 description 10
238000003786 synthesis reaction Methods 0.000 description 10
206010010144 Completed suicide Diseases 0.000 description 9
108010074860 Factor Xa Proteins 0.000 description 9
239000003480 eluent Substances 0.000 description 9
230000035772 mutation Effects 0.000 description 9
238000012360 testing method Methods 0.000 description 9
210000004027 cell Anatomy 0.000 description 8
WBKFWQBXFREOFH-UHFFFAOYSA-N dichloromethane;ethyl acetate Chemical compound ClCCl.CCOC(C)=O WBKFWQBXFREOFH-UHFFFAOYSA-N 0.000 description 8
239000000377 silicon dioxide Substances 0.000 description 8
241000588724 Escherichia coli Species 0.000 description 7
NGNBDVOYPDDBFK-UHFFFAOYSA-N 2-[2,4-di(pentan-2-yl)phenoxy]acetyl chloride Chemical compound CCCC(C)C1=CC=C(OCC(Cl)=O)C(C(C)CCC)=C1 NGNBDVOYPDDBFK-UHFFFAOYSA-N 0.000 description 6
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
238000005119 centrifugation Methods 0.000 description 6
108020001507 fusion proteins Proteins 0.000 description 6
102000037865 fusion proteins Human genes 0.000 description 6
KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 6
108010051423 streptavidin-agarose Proteins 0.000 description 6
108020004705 Codon Proteins 0.000 description 5
241000276498 Pollachius virens Species 0.000 description 5
108010076504 Protein Sorting Signals Proteins 0.000 description 5
238000010367 cloning Methods 0.000 description 5
239000002245 particle Substances 0.000 description 5
150000003457 sulfones Chemical class 0.000 description 5
239000000725 suspension Substances 0.000 description 5
238000005406 washing Methods 0.000 description 5
LWPHUVGDBNUVHA-GXZWQRSESA-N (2,5-dioxopyrrolidin-1-yl) 3-[[3-[2-[5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethylamino]-3-oxopropyl]disulfanyl]propanoate Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)NCCNC(=O)CCSSCCC(=O)ON1C(=O)CCC1=O LWPHUVGDBNUVHA-GXZWQRSESA-N 0.000 description 4
LHNIIDJCEODSHA-OQRUQETBSA-N (6r,7r)-3-[(e)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid Chemical compound N([C@H]1[C@H]2SCC(=C(N2C1=O)C(=O)O)\C=C\C=1C(=CC(=CC=1)[N+]([O-])=O)[N+]([O-])=O)C(=O)CC1=CC=CS1 LHNIIDJCEODSHA-OQRUQETBSA-N 0.000 description 4
NGHVIOIJCVXTGV-ALEPSDHESA-N 6-aminopenicillanic acid Chemical compound [O-]C(=O)[C@H]1C(C)(C)S[C@@H]2[C@H]([NH3+])C(=O)N21 NGHVIOIJCVXTGV-ALEPSDHESA-N 0.000 description 4
NGHVIOIJCVXTGV-UHFFFAOYSA-N 6beta-amino-penicillanic acid Natural products OC(=O)C1C(C)(C)SC2C(N)C(=O)N21 NGHVIOIJCVXTGV-UHFFFAOYSA-N 0.000 description 4
229920000936 Agarose Polymers 0.000 description 4
108020004414 DNA Proteins 0.000 description 4
239000002253 acid Substances 0.000 description 4
238000004458 analytical method Methods 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
230000000295 complement effect Effects 0.000 description 4
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 4
238000002703 mutagenesis Methods 0.000 description 4
231100000350 mutagenesis Toxicity 0.000 description 4
239000006187 pill Substances 0.000 description 4
239000013612 plasmid Substances 0.000 description 4
102000053602 DNA Human genes 0.000 description 3
XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
108010001336 Horseradish Peroxidase Proteins 0.000 description 3
NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 3
239000004793 Polystyrene Substances 0.000 description 3
239000004098 Tetracycline Substances 0.000 description 3
239000007983 Tris buffer Substances 0.000 description 3
229960000723 ampicillin Drugs 0.000 description 3
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
230000001580 bacterial effect Effects 0.000 description 3
230000001588 bifunctional effect Effects 0.000 description 3
238000004587 chromatography analysis Methods 0.000 description 3
238000010276 construction Methods 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
239000000499 gel Substances 0.000 description 3
230000007062 hydrolysis Effects 0.000 description 3
238000006460 hydrolysis reaction Methods 0.000 description 3
238000002372 labelling Methods 0.000 description 3
-1 methoxymethyl ester Chemical class 0.000 description 3
VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
230000005298 paramagnetic effect Effects 0.000 description 3
229920002223 polystyrene Polymers 0.000 description 3
239000002243 precursor Substances 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
238000003756 stirring Methods 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
229960002180 tetracycline Drugs 0.000 description 3
229930101283 tetracycline Natural products 0.000 description 3
235000019364 tetracycline Nutrition 0.000 description 3
150000003522 tetracyclines Chemical class 0.000 description 3
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
238000001262 western blot Methods 0.000 description 3
VHCSBTPOPKFYIU-UHFFFAOYSA-N 2-chloroethanesulfonyl chloride Chemical compound ClCCS(Cl)(=O)=O VHCSBTPOPKFYIU-UHFFFAOYSA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
101710132601 Capsid protein Proteins 0.000 description 2
101710094648 Coat protein Proteins 0.000 description 2
QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
239000004471 Glycine Substances 0.000 description 2
102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 2
241001484259 Lacuna Species 0.000 description 2
101710125418 Major capsid protein Proteins 0.000 description 2
108091028043 Nucleic acid sequence Proteins 0.000 description 2
101710141454 Nucleoprotein Proteins 0.000 description 2
241000283973 Oryctolagus cuniculus Species 0.000 description 2
238000012408 PCR amplification Methods 0.000 description 2
KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
101710083689 Probable capsid protein Proteins 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
230000024932 T cell mediated immunity Effects 0.000 description 2
239000008351 acetate buffer Substances 0.000 description 2
150000007513 acids Chemical class 0.000 description 2
238000001042 affinity chromatography Methods 0.000 description 2
150000001414 amino alcohols Chemical class 0.000 description 2
125000003460 beta-lactamyl group Chemical group 0.000 description 2
101150049515 bla gene Proteins 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
230000008859 change Effects 0.000 description 2
238000012512 characterization method Methods 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
239000012043 crude product Substances 0.000 description 2
OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 description 2
238000010511 deprotection reaction Methods 0.000 description 2
238000011161 development Methods 0.000 description 2
230000018109 developmental process Effects 0.000 description 2
MWFJXRUUPWIALU-UHFFFAOYSA-N ethyl ethenesulfonate Chemical compound CCOS(=O)(=O)C=C MWFJXRUUPWIALU-UHFFFAOYSA-N 0.000 description 2
238000001704 evaporation Methods 0.000 description 2
238000001914 filtration Methods 0.000 description 2
239000012634 fragment Substances 0.000 description 2
230000028996 humoral immune response Effects 0.000 description 2
238000005984 hydrogenation reaction Methods 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
230000005764 inhibitory process Effects 0.000 description 2
238000003780 insertion Methods 0.000 description 2
230000037431 insertion Effects 0.000 description 2
OTCKOJUMXQWKQG-UHFFFAOYSA-L magnesium bromide Chemical compound [Mg+2].[Br-].[Br-] OTCKOJUMXQWKQG-UHFFFAOYSA-L 0.000 description 2
229910001623 magnesium bromide Inorganic materials 0.000 description 2
238000005259 measurement Methods 0.000 description 2
239000012528 membrane Substances 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
238000004091 panning Methods 0.000 description 2
XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 2
238000007747 plating Methods 0.000 description 2
229920001223 polyethylene glycol Polymers 0.000 description 2
239000000843 powder Substances 0.000 description 2
239000002244 precipitate Substances 0.000 description 2
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
238000011084 recovery Methods 0.000 description 2
238000012163 sequencing technique Methods 0.000 description 2
239000002904 solvent Substances 0.000 description 2
230000007480 spreading Effects 0.000 description 2
238000003892 spreading Methods 0.000 description 2
239000000126 substance Substances 0.000 description 2
BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 2
XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
230000002463 transducing effect Effects 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
238000005160 1H NMR spectroscopy Methods 0.000 description 1
GIAFURWZWWWBQT-UHFFFAOYSA-N 2-(2-aminoethoxy)ethanol Chemical compound NCCOCCO GIAFURWZWWWBQT-UHFFFAOYSA-N 0.000 description 1
JKTYGPATCNUWKN-UHFFFAOYSA-N 4-nitrobenzyl alcohol Chemical compound OCC1=CC=C([N+]([O-])=O)C=C1 JKTYGPATCNUWKN-UHFFFAOYSA-N 0.000 description 1
OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
241000283707 Capra Species 0.000 description 1
241000237074 Centris Species 0.000 description 1
XJUZRXYOEPSWMB-UHFFFAOYSA-N Chloromethyl methyl ether Chemical compound COCCl XJUZRXYOEPSWMB-UHFFFAOYSA-N 0.000 description 1
108020004638 Circular DNA Proteins 0.000 description 1
102000029816 Collagenase Human genes 0.000 description 1
108060005980 Collagenase Proteins 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
108090000204 Dipeptidase 1 Proteins 0.000 description 1
238000002965 ELISA Methods 0.000 description 1
101001074568 Enterobacteria phage T4 Host protease inhibitor Proteins 0.000 description 1
241000702374 Enterobacteria phage fd Species 0.000 description 1
241000233866 Fungi Species 0.000 description 1
XJFITURPHAKKAI-SRVKXCTJSA-N His-Pro-Gln Chemical compound C([C@H](N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(N)=O)C(O)=O)C1=CN=CN1 XJFITURPHAKKAI-SRVKXCTJSA-N 0.000 description 1
241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
238000004566 IR spectroscopy Methods 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
108010058683 Immobilized Proteins Proteins 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
229920001213 Polysorbate 20 Polymers 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
238000003436 Schotten-Baumann reaction Methods 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
102220472589 Vitamin K epoxide reductase complex subunit 1_S68A_mutation Human genes 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
125000002252 acyl group Chemical group 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
235000001014 amino acid Nutrition 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
230000003321 amplification Effects 0.000 description 1
230000019552 anatomical structure morphogenesis Effects 0.000 description 1
238000004873 anchoring Methods 0.000 description 1
239000000427 antigen Substances 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
230000008901 benefit Effects 0.000 description 1
HSDAJNMJOMSNEV-UHFFFAOYSA-N benzyl chloroformate Chemical compound ClC(=O)OCC1=CC=CC=C1 HSDAJNMJOMSNEV-UHFFFAOYSA-N 0.000 description 1
102000006635 beta-lactamase Human genes 0.000 description 1
230000003851 biochemical process Effects 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
230000029918 bioluminescence Effects 0.000 description 1
238000005415 bioluminescence Methods 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
239000000969 carrier Substances 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
229960002424 collagenase Drugs 0.000 description 1
239000012230 colorless oil Substances 0.000 description 1
230000000536 complexating effect Effects 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
239000012228 culture supernatant Substances 0.000 description 1
229940099500 cystamine Drugs 0.000 description 1
238000003936 denaturing gel electrophoresis Methods 0.000 description 1
230000008034 disappearance Effects 0.000 description 1
238000004821 distillation Methods 0.000 description 1
XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
230000000763 evoking effect Effects 0.000 description 1
230000029142 excretion Effects 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000007429 general method Methods 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
VANNPISTIUFMLH-UHFFFAOYSA-N glutaric anhydride Chemical compound O=C1CCCC(=O)O1 VANNPISTIUFMLH-UHFFFAOYSA-N 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
230000006872 improvement Effects 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
238000001802 infusion Methods 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000002427 irreversible effect Effects 0.000 description 1
238000002955 isolation Methods 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000006386 neutralization reaction Methods 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
239000003921 oil Substances 0.000 description 1
239000012074 organic phase Substances 0.000 description 1
125000006503 p-nitrobenzyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1[N+]([O-])=O)C([H])([H])* 0.000 description 1
230000020477 pH reduction Effects 0.000 description 1
102000014187 peptide receptors Human genes 0.000 description 1
108010011903 peptide receptors Proteins 0.000 description 1
210000001322 periplasm Anatomy 0.000 description 1
108700010839 phage proteins Proteins 0.000 description 1
239000012071 phase Substances 0.000 description 1
230000035479 physiological effects, processes and functions Effects 0.000 description 1
231100000614 poison Toxicity 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
239000011148 porous material Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
210000004777 protein coat Anatomy 0.000 description 1
230000006337 proteolytic cleavage Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
239000012264 purified product Substances 0.000 description 1
239000012429 reaction media Substances 0.000 description 1
230000010076 replication Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000007858 starting material Substances 0.000 description 1
YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 description 1
230000004083 survival effect Effects 0.000 description 1
229960003080 taurine Drugs 0.000 description 1
239000003440 toxic substance Substances 0.000 description 1
230000009466 transformation Effects 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
230000035899 viability Effects 0.000 description 1
210000002845 virion Anatomy 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
- C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10051—Methods of production or purification of viral material

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
Toxicology (AREA)
Analytical Chemistry (AREA)
Plant Pathology (AREA)
Virology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

NO19941731A 1991-11-29 1994-05-10 Fremgangsmåte for utvelgelse av rekombinante mikroorganismer hvis overflate omfatter i det minste et molekyl med enzymatiskaktivitet NO313593B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
BE9101106A BE1006312A3 (fr)	1991-11-29	1991-11-29	Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique.
PCT/BE1992/000052 WO1993011242A1 (fr)	1991-11-29	1992-11-30	Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique

Publications (3)

Publication Number	Publication Date
NO941731D0 NO941731D0 (no)	1994-05-10
NO941731L NO941731L (no)	1994-06-21
NO313593B1 true NO313593B1 (no)	2002-10-28

Family

ID=3885823

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO19941731A NO313593B1 (no)	1991-11-29	1994-05-10	Fremgangsmåte for utvelgelse av rekombinante mikroorganismer hvis overflate omfatter i det minste et molekyl med enzymatiskaktivitet

Country Status (16)

Country	Link
EP (1)	EP0617737B1 (de)
JP (1)	JP4125364B2 (de)
KR (1)	KR100243997B1 (de)
AT (1)	ATE147783T1 (de)
AU (1)	AU671089B2 (de)
BE (1)	BE1006312A3 (de)
BR (1)	BR9206970A (de)
CA (1)	CA2124580C (de)
DE (1)	DE69216853T2 (de)
DK (1)	DK0617737T3 (de)
ES (1)	ES2098555T3 (de)
FI (1)	FI109131B (de)
HU (1)	HU220876B1 (de)
NO (1)	NO313593B1 (de)
SG (1)	SG48094A1 (de)
WO (1)	WO1993011242A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL104829A (en) *	1992-02-24	1999-04-11	Igen Inc	Selection is based on a response to the expression and concentration of catalytic units
EP1169473B1 (de) *	1999-04-14	2006-11-22	THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES	Hochsensitiver nachweis von biomolekülen mittels "phage display"

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5325030B2 (de) *	1972-12-19	1978-07-24
US4165258A (en) *	1975-10-08	1979-08-21	University Of Pennsylvania	Plasminogen activating enzyme-specific competitive inhibitor
EP0298094A1 (de) *	1986-12-01	1989-01-11	McDONNELL DOUGLAS CORPORATION	Verfahren zur identifizierung von salmonella
DE3833628A1 (de) *	1988-10-03	1990-04-12	Genlux Forschungsgesellschaft	Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
EP0437537B1 (de) *	1988-10-04	1997-02-26	DNA Plant Technology Corporation	Bakterieller nachweis durch phagentransduktion von nachweisbaren phenotypen
JP2810138B2 (ja) *	1989-08-23	1998-10-15	昭和電工株式会社	酵素活性測定用充填剤、その使用方法および装置
EP0423938B1 (de) *	1989-09-29	1994-03-02	Rohm And Haas Company	Ligand enthaltendes Medium für chromatographische Trennung, Verfahren zur dessen Herstellung und dessen Verwendung zur Isolierung von synthetischen oder natürlichen Molekülen von einer Fluidmischung

1991
- 1991-11-29 BE BE9101106A patent/BE1006312A3/fr not_active IP Right Cessation
1992
- 1992-11-30 ES ES92923617T patent/ES2098555T3/es not_active Expired - Lifetime
- 1992-11-30 DK DK92923617.2T patent/DK0617737T3/da active
- 1992-11-30 EP EP92923617A patent/EP0617737B1/de not_active Expired - Lifetime
- 1992-11-30 SG SG1996007002A patent/SG48094A1/en unknown
- 1992-11-30 CA CA002124580A patent/CA2124580C/en not_active Expired - Lifetime
- 1992-11-30 AT AT92923617T patent/ATE147783T1/de active
- 1992-11-30 WO PCT/BE1992/000052 patent/WO1993011242A1/fr not_active Ceased
- 1992-11-30 DE DE69216853T patent/DE69216853T2/de not_active Expired - Lifetime
- 1992-11-30 AU AU29373/92A patent/AU671089B2/en not_active Expired
- 1992-11-30 JP JP50965793A patent/JP4125364B2/ja not_active Expired - Lifetime
- 1992-11-30 HU HU9401593A patent/HU220876B1/hu unknown
- 1992-11-30 KR KR1019940701814A patent/KR100243997B1/ko not_active Expired - Fee Related
- 1992-11-30 BR BR9206970A patent/BR9206970A/pt not_active IP Right Cessation
1994
- 1994-05-10 NO NO19941731A patent/NO313593B1/no not_active IP Right Cessation
- 1994-05-26 FI FI942469A patent/FI109131B/fi not_active IP Right Cessation

Also Published As

Publication number	Publication date
ES2098555T3 (es)	1997-05-01
DE69216853T2 (de)	1997-06-26
FI942469A0 (fi)	1994-05-26
CA2124580A1 (en)	1993-06-10
AU2937392A (en)	1993-06-28
BR9206970A (pt)	1995-12-19
JPH07505999A (ja)	1995-07-06
HUT70310A (en)	1995-09-28
HU220876B1 (en)	2002-06-29
BE1006312A3 (fr)	1994-07-19
NO941731L (no)	1994-06-21
FI942469L (fi)	1994-05-26
DE69216853D1 (de)	1997-02-27
NO941731D0 (no)	1994-05-10
JP4125364B2 (ja)	2008-07-30
DK0617737T3 (da)	1997-07-07
EP0617737A1 (de)	1994-10-05
CA2124580C (en)	2002-07-16
EP0617737B1 (de)	1997-01-15
HU9401593D0 (en)	1994-08-29
FI109131B (fi)	2002-05-31
SG48094A1 (en)	1998-04-17
AU671089B2 (en)	1996-08-15
ATE147783T1 (de)	1997-02-15
WO1993011242A1 (fr)	1993-06-10
KR100243997B1 (ko)	2000-02-01

Legal Events

Date	Code	Title	Description
2012-12-10	MK1K	Patent expired

Publication	Publication Date	Title
JP6075658B2 (ja)	2017-02-08	方法及び組成物
Soumillion et al.	1994	Selection of β-lactamase on filamentous bacteriophage by catalytic activity
US5338665A (en)	1994-08-16	Peptide library and screening method
US8969253B2 (en)	2015-03-03	Method for screening phage display libraries against each other
US12371817B2 (en)	2025-07-29	Methods of making and utilizing amber-obligated phage display libraries
NO313593B1 (no)	2002-10-28	Fremgangsmåte for utvelgelse av rekombinante mikroorganismer hvis overflate omfatter i det minste et molekyl med enzymatiskaktivitet
US20060078875A1 (en)	2006-04-13	Genetic selection of small molecule modulators of protein-protein interactions
WO1995020601A1 (en)	1995-08-03	Reagents binding vinculin, dynein, and glutathione s-transferase from peptide libraries
Rees	2016	Identifying peptides that bind to human serum albumin using phage display for the development of sensors that detect injury in military personnel
Zhang	2012	Protein engineering and gene profiling by phage display and yeast surface display