NO794133L - Dna-plasmid. - Google Patents
Dna-plasmid.Info
- Publication number
- NO794133L NO794133L NO794133A NO794133A NO794133L NO 794133 L NO794133 L NO 794133L NO 794133 A NO794133 A NO 794133A NO 794133 A NO794133 A NO 794133A NO 794133 L NO794133 L NO 794133L
- Authority
- NO
- Norway
- Prior art keywords
- dna
- plasmid
- copy number
- elements
- mutants
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 91
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 24
- 239000012634 fragment Substances 0.000 claims abstract description 19
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 17
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 9
- 238000005520 cutting process Methods 0.000 claims abstract description 8
- 102000004594 DNA Polymerase I Human genes 0.000 claims abstract 3
- 108010017826 DNA Polymerase I Proteins 0.000 claims abstract 3
- 238000010367 cloning Methods 0.000 claims description 12
- 230000014616 translation Effects 0.000 claims description 11
- 230000000977 initiatory effect Effects 0.000 claims description 7
- 239000003112 inhibitor Substances 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000013519 translation Methods 0.000 claims description 3
- 230000029087 digestion Effects 0.000 claims description 2
- 238000005304 joining Methods 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000002950 deficient Effects 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 abstract description 5
- 230000010076 replication Effects 0.000 abstract description 5
- 230000008707 rearrangement Effects 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 41
- 108010020764 Transposases Proteins 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 16
- 102000008579 Transposases Human genes 0.000 description 15
- 238000012217 deletion Methods 0.000 description 14
- 230000037430 deletion Effects 0.000 description 14
- 230000035772 mutation Effects 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 210000000349 chromosome Anatomy 0.000 description 7
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 108060002716 Exonuclease Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- 108010054278 Lac Repressors Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229940123573 Protein synthesis inhibitor Drugs 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- -1 nucleotide triphosphates Chemical class 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000007 protein synthesis inhibitor Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 101150116497 sacm1l gene Proteins 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97270578A | 1978-12-26 | 1978-12-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO794133L true NO794133L (no) | 1980-06-27 |
Family
ID=25520026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO794133A NO794133L (no) | 1978-12-26 | 1979-12-18 | Dna-plasmid. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0013830B1 (de) |
| JP (1) | JPS55104888A (de) |
| AT (1) | ATE2340T1 (de) |
| AU (1) | AU531722B2 (de) |
| DE (1) | DE2964664D1 (de) |
| DK (1) | DK541979A (de) |
| ES (1) | ES487236A0 (de) |
| FI (1) | FI793884A7 (de) |
| NO (1) | NO794133L (de) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4966840A (en) * | 1978-12-26 | 1990-10-30 | Cetus Corporation | Stable high copy number plasmids |
| ZA811368B (en) * | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
| CA1198067A (en) * | 1981-02-27 | 1985-12-17 | David H. Gelfand | Stable high copy number plasmids |
| CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
| JPH0753111B2 (ja) * | 1982-09-16 | 1995-06-07 | ベンツォン ファーマ エイ/エス | 条件非制御の複製挙動を持つプラスミド |
| US4710473A (en) * | 1983-08-10 | 1987-12-01 | Amgen, Inc. | DNA plasmids |
| HU194307B (en) * | 1983-09-16 | 1988-01-28 | Richter Gedeon Vegyeszet | Process for producing plasmidvectors of high copy number |
| EP0151760A1 (de) * | 1984-01-06 | 1985-08-21 | Takeda Chemical Industries, Ltd. | DNA und ihre Verwendung |
| GB2166743B (en) * | 1984-11-13 | 1989-01-05 | Solvay | Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression |
| KR20220066384A (ko) * | 2019-09-25 | 2022-05-24 | 카톨리에케 유니버시테이트 루벤 | 대형 벡터 및 고수율 제조를 위한 방법 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2759053A1 (de) * | 1977-12-30 | 1979-07-12 | Uhlin | Verfahren zum herstellen von genprodukten von plasmid-dns |
-
1979
- 1979-12-12 FI FI793884A patent/FI793884A7/fi not_active Application Discontinuation
- 1979-12-17 AU AU53922/79A patent/AU531722B2/en not_active Ceased
- 1979-12-18 NO NO794133A patent/NO794133L/no unknown
- 1979-12-19 DK DK541979A patent/DK541979A/da not_active Application Discontinuation
- 1979-12-21 AT AT79303035T patent/ATE2340T1/de not_active IP Right Cessation
- 1979-12-21 DE DE7979303035T patent/DE2964664D1/de not_active Expired
- 1979-12-21 EP EP79303035A patent/EP0013830B1/de not_active Expired
- 1979-12-24 ES ES487236A patent/ES487236A0/es active Granted
- 1979-12-26 JP JP17018979A patent/JPS55104888A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ES8107301A1 (es) | 1980-12-16 |
| FI793884A7 (fi) | 1981-01-01 |
| DK541979A (da) | 1980-06-27 |
| AU5392279A (en) | 1980-07-03 |
| AU531722B2 (en) | 1983-09-01 |
| EP0013830A2 (de) | 1980-08-06 |
| EP0013830A3 (en) | 1980-11-12 |
| ATE2340T1 (de) | 1983-02-15 |
| DE2964664D1 (en) | 1983-03-03 |
| ES487236A0 (es) | 1980-12-16 |
| JPS55104888A (en) | 1980-08-11 |
| EP0013830B1 (de) | 1983-01-26 |
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