NO841130L - Ledersekvens for aa fremme sekresjon av genprodukter - Google Patents

Ledersekvens for aa fremme sekresjon av genprodukter

Info

Publication number: NO841130L
Authority: NO; Norway
Prior art keywords: transfer vector; stated; leader sequence; gene; structural gene
Prior art date: 1983-03-28

Application number

NO841130A

Other languages

English (en)

Norwegian (no)

Inventor

Anthony Atkinson

Nigel Peter Minton

Roger Franklin Sherwood

Original Assignee

Health Lab Service Board

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-03-28

Filing date

1984-03-22

Publication date

1984-10-01

1984-03-22 Application filed by Health Lab Service Board filed Critical Health Lab Service Board

1984-10-01 Publication of NO841130L publication Critical patent/NO841130L/no

Links

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230000000717 retained effect Effects 0.000 description 1
239000012266 salt solution Substances 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
239000013049 sediment Substances 0.000 description 1
238000004062 sedimentation Methods 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
239000013605 shuttle vector Substances 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000000527 sonication Methods 0.000 description 1
239000000600 sorbitol Substances 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
238000010189 synthetic method Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
150000005691 triesters Chemical class 0.000 description 1
238000002604 ultrasonography Methods 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1
239000011592 zinc chloride Substances 0.000 description 1
235000005074 zinc chloride Nutrition 0.000 description 1
JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/17—Metallocarboxypeptidases (3.4.17)
- C12Y304/17011—Glutamate carboxypeptidase (3.4.17.11)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/485—Exopeptidases (3.4.11-3.4.19)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Time Recorders, Dirve Recorders, Access Control (AREA)

NO841130A 1983-03-28 1984-03-22 Ledersekvens for aa fremme sekresjon av genprodukter NO841130L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
GB8308483A GB8308483D0 (en)	1983-03-28	1983-03-28	Secretion of gene products

Publications (1)

Publication Number	Publication Date
NO841130L true NO841130L (no)	1984-10-01

Family

ID=10540349

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO841130A NO841130L (no)	1983-03-28	1984-03-22	Ledersekvens for aa fremme sekresjon av genprodukter

Country Status (6)

Country	Link
EP (1)	EP0121352B1 (da)
JP (1)	JPS6054686A (da)
DE (1)	DE3468434D1 (da)
DK (1)	DK167584A (da)
GB (1)	GB8308483D0 (da)
NO (1)	NO841130L (da)

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US4925919A (en) *	1984-04-25	1990-05-15	Roland Mertelsmann	Purified interleukin 2
US4853332A (en) *	1982-10-19	1989-08-01	Cetus Corporation	Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of biologically active proteins
US4711844A (en) *	1983-03-09	1987-12-08	Cetus Corporation	Modified signal peptides
DE3486216T2 (de) *	1983-04-25	1994-02-17	Chiron Corp	Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
IL71991A (en) *	1983-06-06	1994-05-30	Genentech Inc	Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
US4569790A (en) *	1984-03-28	1986-02-11	Cetus Corporation	Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4908433A (en) *	1984-04-25	1990-03-13	Sloan-Kettering Institute For Cancer Research	Uses of interleukin-2
US4908434A (en) *	1984-04-25	1990-03-13	Sloan-Kettering Institute For Cancer Research	Process for preparing purified interleukin-2
US4963495A (en) *	1984-10-05	1990-10-16	Genentech, Inc.	Secretion of heterologous proteins
US4569794A (en) *	1984-12-05	1986-02-11	Eli Lilly And Company	Process for purifying proteins and compounds useful in such process
US4935352A (en) *	1985-10-21	1990-06-19	Takeda Chemical Industries, Ltd.	Expression vector for animal cell line and use thereof
JPH07108224B2 (ja) *	1985-11-07	1995-11-22	重三鵜高	バチルス・ブレビスを用いる遺伝子の発現方法
JPH0669377B2 (ja) *	1985-11-25	1994-09-07	工業技術院長	Ｄｎａ塩基配列およびベクタ−
JPS6387975A (ja) *	1986-10-02	1988-04-19	Agency Of Ind Science & Technol	枯草菌菌株
US4987070A (en) *	1987-03-04	1991-01-22	Suntory Limited	Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins
ATE138102T1 (de) *	1991-02-19	1996-06-15	Takeda Chemical Industries Ltd	Verfahren zur herstellung von cysteinfreien peptiden
GB9301553D0 (en) *	1993-01-27	1993-03-17	Univ Wales	Protein synthesis
GB9415167D0 (en) *	1994-07-27	1994-09-14	Springer Caroline J	Improvements relating to cancer therapy
US6410701B1 (en) *	1995-05-05	2002-06-25	Human Genome Sciences, Inc.	Human neuropeptide receptor
GB9601640D0 (en) *	1996-01-26	1996-03-27	Cancer Res Campaign Tech	Ligand directed enzyme prodrug therapy
US6656718B2 (en)	2000-07-07	2003-12-02	Cancer Research Technology Limited	Modified carboxypeptidase enzymes and their use
US9453251B2 (en)	2002-10-08	2016-09-27	Pfenex Inc.	Expression of mammalian proteins in Pseudomonas fluorescens
US7153666B2 (en)	2003-07-17	2006-12-26	General Atomics	Methods and compositions for determination of glycated proteins
US7022494B2 (en)	2003-09-19	2006-04-04	General Atomics	Detection of potassium ions using ion-sensitive enzymes
US8187831B2 (en)	2003-09-19	2012-05-29	General Atomics	Determination of ions using ion-sensitive enzymes
CN1882605B (zh)	2003-11-21	2012-07-11	陶氏环球技术公司	使用Sec-系统分泌的改进的表达系统
US7355027B2 (en) *	2004-06-16	2008-04-08	Dynport Vaccine Company Llc	Bacillus anthracis protective antigen
US8101735B2 (en)	2004-06-16	2012-01-24	Health Protection Agency	Preparation of protective antigen
EP1774017B1 (en)	2004-07-26	2013-05-15	Pfenex Inc.	Process for improved protein expression by strain engineering
US7943385B2 (en)	2006-07-25	2011-05-17	General Atomics	Methods for assaying percentage of glycated hemoglobin
WO2008013874A1 (en)	2006-07-25	2008-01-31	General Atomics	Methods for assaying percentage of glycated hemoglobin
CA2677179C (en)	2007-01-31	2016-02-16	Dow Global Technologies Inc.	Bacterial leader sequences for increased expression
US9580719B2 (en)	2007-04-27	2017-02-28	Pfenex, Inc.	Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en)	2007-04-27	2016-07-19	Pfenex Inc.	Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2283149A1 (en)	2008-05-13	2011-02-16	General Atomics	Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
HK1222331A1 (zh)	2013-03-12	2017-06-30	通用医疗公司	修饰的缪勒抑制物质(mis)蛋白及其用於疾病治疗的用途
US11135269B2 (en)	2013-12-11	2021-10-05	The General Hospital Corporation	Use of mullerian inhibiting substance (MIS) proteins for contraception and ovarian reserve preservation

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GR71687B (da) *	1977-11-08	1983-06-21	Genentech Inc
US4506013A (en) *	1980-10-03	1985-03-19	Eli Lilly And Company	Stabilizing and selecting recombinant DNA host cells

1983
- 1983-03-28 GB GB8308483A patent/GB8308483D0/en active Pending
1984
- 1984-03-06 DE DE8484301468T patent/DE3468434D1/de not_active Expired
- 1984-03-06 EP EP19840301468 patent/EP0121352B1/en not_active Expired
- 1984-03-22 NO NO841130A patent/NO841130L/no unknown
- 1984-03-26 DK DK167584A patent/DK167584A/da not_active IP Right Cessation
- 1984-03-27 JP JP59059287A patent/JPS6054686A/ja active Pending

Also Published As

Publication number	Publication date
EP0121352A1 (en)	1984-10-10
GB8308483D0 (en)	1983-05-05
JPS6054686A (ja)	1985-03-29
DK167584D0 (da)	1984-03-26
DE3468434D1 (en)	1988-02-11
DK167584A (da)	1984-09-29
EP0121352B1 (en)	1988-01-07

Publication	Publication Date	Title
EP0121352B1 (en)	1988-01-07	A leader sequence to promote the secretion of gene products
EP0096430B1 (en)	1990-01-10	Cloning system for kluyveromyces species
Shevchik et al.	1994	Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activity.
Reverchon et al.	1994	pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi
CN111278852B (zh)	2024-07-16	重组欧氏杆菌天冬酰胺酶的生产方法
US4769326A (en)	1988-09-06	Expression linkers
JP2963695B2 (ja)	1999-10-18	クローン化リゾスタフィン遺伝子の発現
JP3878207B2 (ja)	2007-02-07	ｏｓｍＢプロモータで制御される発現プラスミド
NO840200L (no)	1984-07-30	Glukoamylase cdna.
EP0035384A2 (en)	1981-09-09	Deoxynucleotide linkers to be attached to a cloned DNA coding sequence
EP0889134A1 (en)	1999-01-07	$g(b)-FRUCTOFURANOSIDASE AND ITS GENE, METHOD OF ISOLATING $g(b)-FRUCTOFURANOSIDASE GENE, SYSTEM FOR PRODUCING $g(b)-FRUCTOFURANOSIDASE, AND $g(b)-FRUCTOFURANOSIDASE VARIANT
WO2019035058A1 (en)	2019-02-21	SYSTEMS AND METHODS FOR THE PRODUCTION OF DIPHERYTIC TOXIN POLYPEPTIDES
Beuve et al.	1990	Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases
EP0353188B1 (en)	1994-03-16	Novel expression system
Delgado et al.	2003	Candida albicans TDH3 gene promotes secretion of internal invertase when expressed in Saccharomyces cerevisiae as a glyceraldehyde‐3‐phosphate dehydrogenase‐invertase fusion protein
EP0302838B1 (en)	1994-06-29	Cloning of the gene coding the isoamylase enzyme and its use in the production of said enzyme
CN113073107B (zh)	2022-10-14	一种甘露聚糖酶基因AbMan5、重组表达质粒、重组表达菌株、甘露聚糖酶及其应用
CN115161299B (zh)	2023-09-05	一种158位突变的醛酮还原酶突变体及其应用
EP0190252A1 (en)	1986-08-13	Recombinant dna molecule, transformed microorganisms and process for producing penicillin v amidase
KR100420313B1 (ko)	2004-03-03	신규 레반슈크라제를 이용한 레반의 생산방법
CA1200775A (en)	1986-02-18	Expression linkers
Walker et al.	1990	Deletion analysis of domain independence in the TRP1 gene product of Neurospora crassa
JPH05500309A (ja)	1993-01-28	新規のポリガラクツロナーゼ遺伝子および利用方法
JPH02219570A (ja)	1990-09-03	ヒト５―リポキシゲナーゼの製造方法
JPH09168389A (ja)	1997-06-30	アスコルビン酸オキシダーゼをコードするｄｎａ