NO850493L - Heterologt paavisningssystem - Google Patents

Heterologt paavisningssystem

Info

Publication number: NO850493L
Authority: NO; Norway
Prior art keywords: biotinylated; lectin; enzyme; complex; streptavidin
Prior art date: 1984-02-09

Application number

NO850493A

Other languages

English (en)

Norwegian (no)

Inventor

Elazar Rabbani

Original Assignee

Enzo Biochem Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-02-09

Filing date

1985-02-08

Publication date

1985-08-12

1985-02-08 Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc

1985-08-12 Publication of NO850493L publication Critical patent/NO850493L/no

Links

239000002523 lectin Substances 0.000 claims abstract description 75
108090001090 Lectins Proteins 0.000 claims abstract description 74
102000004856 Lectins Human genes 0.000 claims abstract description 74
102000004190 Enzymes Human genes 0.000 claims abstract description 65
108090000790 Enzymes Proteins 0.000 claims abstract description 65
108020004414 DNA Proteins 0.000 claims abstract description 63
108090001008 Avidin Proteins 0.000 claims abstract description 37
229920002307 Dextran Polymers 0.000 claims abstract description 29
108090000288 Glycoproteins Proteins 0.000 claims abstract description 24
102000003886 Glycoproteins Human genes 0.000 claims abstract description 24
108010090804 Streptavidin Proteins 0.000 claims abstract description 23
102000053602 DNA Human genes 0.000 claims abstract description 10
150000004676 glycans Chemical class 0.000 claims abstract description 8
125000003147 glycosyl group Chemical group 0.000 claims abstract description 8
229920001282 polysaccharide Polymers 0.000 claims abstract description 8
239000005017 polysaccharide Substances 0.000 claims abstract description 8
150000001720 carbohydrates Chemical class 0.000 claims abstract 7
238000000034 method Methods 0.000 claims description 19
239000002773 nucleotide Substances 0.000 claims description 16
125000003729 nucleotide group Chemical group 0.000 claims description 16
108091028043 Nucleic acid sequence Proteins 0.000 claims 1
238000001514 detection method Methods 0.000 abstract description 41
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract description 40
239000011616 biotin Substances 0.000 abstract description 32
229960002685 biotin Drugs 0.000 abstract description 32
238000009739 binding Methods 0.000 abstract description 20
235000020958 biotin Nutrition 0.000 abstract description 20
108010062580 Concanavalin A Proteins 0.000 abstract description 17
108010001336 Horseradish Peroxidase Proteins 0.000 abstract description 16
235000000346 sugar Nutrition 0.000 abstract description 14
239000000463 material Substances 0.000 abstract description 11
238000006243 chemical reaction Methods 0.000 abstract description 7
239000012620 biological material Substances 0.000 abstract description 6
102000003992 Peroxidases Human genes 0.000 abstract description 3
108020004682 Single-Stranded DNA Proteins 0.000 abstract description 3
230000000295 complement effect Effects 0.000 abstract description 3
HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 abstract description 2
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 abstract description 2
102000013563 Acid Phosphatase Human genes 0.000 abstract description 2
108010051457 Acid Phosphatase Proteins 0.000 abstract description 2
108040007629 peroxidase activity proteins Proteins 0.000 abstract description 2
229940088598 enzyme Drugs 0.000 description 54
230000027455 binding Effects 0.000 description 19
108010062540 Chorionic Gonadotropin Proteins 0.000 description 12
102000011022 Chorionic Gonadotropin Human genes 0.000 description 12
229940084986 human chorionic gonadotropin Drugs 0.000 description 12
239000011324 bead Substances 0.000 description 10
239000000758 substrate Substances 0.000 description 10
239000000872 buffer Substances 0.000 description 9
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 9
239000000523 sample Substances 0.000 description 9
229930186217 Glycolipid Natural products 0.000 description 8
102000004169 proteins and genes Human genes 0.000 description 7
108090000623 proteins and genes Proteins 0.000 description 7
239000013077 target material Substances 0.000 description 7
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
239000007787 solid Substances 0.000 description 6
239000000020 Nitrocellulose Substances 0.000 description 4
229920002684 Sepharose Polymers 0.000 description 4
238000004458 analytical method Methods 0.000 description 4
210000000170 cell membrane Anatomy 0.000 description 4
238000002474 experimental method Methods 0.000 description 4
238000009396 hybridization Methods 0.000 description 4
229920001220 nitrocellulos Polymers 0.000 description 4
150000003839 salts Chemical class 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
230000015572 biosynthetic process Effects 0.000 description 3
230000000903 blocking effect Effects 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 2
108020004635 Complementary DNA Proteins 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
239000013504 Triton X-100 Substances 0.000 description 2
229920004890 Triton X-100 Polymers 0.000 description 2
239000011149 active material Substances 0.000 description 2
WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 2
230000003321 amplification Effects 0.000 description 2
239000000427 antigen Substances 0.000 description 2
102000036639 antigens Human genes 0.000 description 2
108091007433 antigens Proteins 0.000 description 2
238000010804 cDNA synthesis Methods 0.000 description 2
239000000969 carrier Substances 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
230000002860 competitive effect Effects 0.000 description 2
239000002299 complementary DNA Substances 0.000 description 2
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
239000003112 inhibitor Substances 0.000 description 2
239000000787 lecithin Substances 0.000 description 2
229940067606 lecithin Drugs 0.000 description 2
235000010445 lecithin Nutrition 0.000 description 2
108010034897 lentil lectin Proteins 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
230000009871 nonspecific binding Effects 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
150000002482 oligosaccharides Chemical class 0.000 description 2
239000002953 phosphate buffered saline Substances 0.000 description 2
239000000047 product Substances 0.000 description 2
239000000243 solution Substances 0.000 description 2
150000008163 sugars Chemical class 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
239000004094 surface-active agent Substances 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
102100026189 Beta-galactosidase Human genes 0.000 description 1
235000002566 Capsicum Nutrition 0.000 description 1
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
241000196324 Embryophyta Species 0.000 description 1
108010015776 Glucose oxidase Proteins 0.000 description 1
239000004366 Glucose oxidase Substances 0.000 description 1
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
102000006386 Myelin Proteins Human genes 0.000 description 1
108010083674 Myelin Proteins Proteins 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
239000006002 Pepper Substances 0.000 description 1
235000016761 Piper aduncum Nutrition 0.000 description 1
235000017804 Piper guineense Nutrition 0.000 description 1
244000203593 Piper nigrum Species 0.000 description 1
235000008184 Piper nigrum Nutrition 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
LEBBDRXHHNYZIA-LDUWYPJVSA-N [(2s,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] n-[(z)-1,3-dihydroxyoctadec-4-en-2-yl]carbamate Chemical compound CCCCCCCCCCCCC\C=C/C(O)C(CO)NC(=O)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O LEBBDRXHHNYZIA-LDUWYPJVSA-N 0.000 description 1
108010070626 acid beta-galactosidase Proteins 0.000 description 1
WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
230000006287 biotinylation Effects 0.000 description 1
238000007413 biotinylation Methods 0.000 description 1
239000012876 carrier material Substances 0.000 description 1
210000004027 cell Anatomy 0.000 description 1
230000009918 complex formation Effects 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
230000001086 cytosolic effect Effects 0.000 description 1
238000012631 diagnostic technique Methods 0.000 description 1
108010039433 dolichos biflorus agglutinin Proteins 0.000 description 1
230000000694 effects Effects 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
229930182830 galactose Natural products 0.000 description 1
150000002270 gangliosides Chemical class 0.000 description 1
239000008103 glucose Substances 0.000 description 1
229940116332 glucose oxidase Drugs 0.000 description 1
235000019420 glucose oxidase Nutrition 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
239000005556 hormone Substances 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000002427 irreversible effect Effects 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000008101 lactose Substances 0.000 description 1
239000003446 ligand Substances 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
210000005012 myelin Anatomy 0.000 description 1
229920001542 oligosaccharide Polymers 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
230000011664 signaling Effects 0.000 description 1
238000005728 strengthening Methods 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
150000003641 trioses Chemical class 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
239000011534 wash buffer Substances 0.000 description 1
238000005406 washing Methods 0.000 description 1
238000001262 western blot Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/827—Lectins

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Hematology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Microbiology (AREA)
Urology & Nephrology (AREA)
Analytical Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Physics & Mathematics (AREA)
Pathology (AREA)
Genetics & Genomics (AREA)
Cell Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
General Physics & Mathematics (AREA)
General Engineering & Computer Science (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Control Of Indicators Other Than Cathode Ray Tubes (AREA)
Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
Controls And Circuits For Display Device (AREA)
Saccharide Compounds (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

NO850493A 1984-02-09 1985-02-08 Heterologt paavisningssystem NO850493L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US57873284A	1984-02-09	1984-02-09

Publications (1)

Publication Number	Publication Date
NO850493L true NO850493L (no)	1985-08-12

Family

ID=24314075

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO850493A NO850493L (no)	1984-02-09	1985-02-08	Heterologt paavisningssystem

Country Status (11)

Country	Link
US (1)	US4889798A (de)
EP (1)	EP0151492B1 (de)
JP (1)	JPS60188095A (de)
AT (1)	ATE133261T1 (de)
AU (1)	AU3844485A (de)
CA (1)	CA1314810C (de)
DE (1)	DE3588078T2 (de)
DK (1)	DK61085A (de)
ES (2)	ES8802188A1 (de)
IL (1)	IL74262A0 (de)
NO (1)	NO850493L (de)

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US4957858A (en) *	1986-04-16	1990-09-18	The Salk Instute For Biological Studies	Replicative RNA reporter systems
ATE107031T1 (de) *	1984-12-03	1994-06-15	Hoechst Celanese Corp	Verfahren zur bestimmung eines liganden.
US5273882A (en) *	1985-06-13	1993-12-28	Amgen	Method and kit for performing nucleic acid hybridization assays
IL79112A (en) *	1985-06-13	1992-01-15	Abbott Lab	Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay
US4724203A (en) *	1985-10-04	1988-02-09	Miles Laboratories, Inc.	Caproylamidobiotinylated peroxidase
US5817797A (en) *	1988-06-01	1998-10-06	The United States Of America As Represented By The Department Of Health And Human Services	Sequencing DNA; a modification of the polymerase chain reaction
CA2002076A1 (en) *	1988-11-21	1990-05-21	Brent A. Burdick	Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
GB2239948B (en) *	1989-12-28	1994-01-12	Aisin Seiki	Fluorescence assay using phosphatase and a naphthol phosphate derivative
JP2646814B2 (ja) *	1990-08-07	1997-08-27	アイシン精機株式会社	核酸等の検定方法
US5264343A (en) *	1990-08-31	1993-11-23	Eleanor Roosevelt Institute	Method for distinguishing normal and cancer cells
WO1993005184A1 (en) *	1991-09-10	1993-03-18	Love Jack D	Dna/rna target and signal amplification
US5329461A (en) *	1992-07-23	1994-07-12	Acrogen, Inc.	Digital analyte detection system
JPH07165800A (ja) *	1993-12-08	1995-06-27	Res Dev Corp Of Japan	人工蛋白質超分子とその製造法
US5783387A (en) *	1995-02-06	1998-07-21	The Regents Of The University Of California	Method for identifying and quantifying nucleic acid sequence aberrations
US5731153A (en) *	1996-08-26	1998-03-24	The Regents Of The University Of California	Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions
US6096508A (en) *	1995-08-16	2000-08-01	Kirkegaard & Perry Laboratoies, Inc.	Method of reducing background in biotin-based assays
DE19724787A1 (de) *	1997-06-06	1998-12-10	Biotez Berlin Buch Gmbh Bioche	Streptavidin/Avidin beschichtete Oberflächen
CA2313641A1 (en) *	1997-12-12	1999-06-24	Digene Corporation	Universal collection medium
KR20000074881A (ko) *	1999-05-26	2000-12-15	황승용	마이크로웰을 이용한 디엔에이 돌연변이의 확인방법 및 키트
JP3781934B2 (ja) *	1999-12-22	2006-06-07	株式会社ニチレイバイオサイエンス	酵素−タンパク質複合体
US7102024B1 (en)	2000-08-01	2006-09-05	Schwartz David A	Functional biopolymer modification reagents and uses thereof
US6686461B1 (en)	2000-03-22	2004-02-03	Solulink Bioscience, Inc.	Triphosphate oligonucleotide modification reagents and uses thereof
US7601497B2 (en)	2000-06-15	2009-10-13	Qiagen Gaithersburg, Inc.	Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en)	2000-06-15	2008-10-21	Digene Corporation	Detection of nucleic acids by type-specific hybrid capture method
CA2705985A1 (en)	2007-11-28	2009-06-04	Great Basin Scientific	Methods and compositions for signal enhancement using multivalent interactions
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1985
- 1985-02-05 AU AU38444/85A patent/AU3844485A/en not_active Abandoned
- 1985-02-06 IL IL74262A patent/IL74262A0/xx unknown
- 1985-02-07 CA CA000473830A patent/CA1314810C/en not_active Expired - Lifetime
- 1985-02-07 JP JP60020996A patent/JPS60188095A/ja active Pending
- 1985-02-08 DK DK61085A patent/DK61085A/da not_active Application Discontinuation
- 1985-02-08 AT AT85101353T patent/ATE133261T1/de not_active IP Right Cessation
- 1985-02-08 NO NO850493A patent/NO850493L/no unknown
- 1985-02-08 ES ES540250A patent/ES8802188A1/es not_active Expired
- 1985-02-08 DE DE3588078T patent/DE3588078T2/de not_active Expired - Lifetime
- 1985-02-08 EP EP85101353A patent/EP0151492B1/de not_active Expired - Lifetime
1986
- 1986-04-16 ES ES554045A patent/ES8800439A1/es not_active Expired
1987
- 1987-02-13 US US07/015,563 patent/US4889798A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DK61085A (da)	1985-08-10
ES540250A0 (es)	1988-04-01
ES8800439A1 (es)	1987-10-16
EP0151492B1 (de)	1996-01-17
IL74262A0 (en)	1985-05-31
DK61085D0 (da)	1985-02-08
DE3588078T2 (de)	1996-09-19
EP0151492A2 (de)	1985-08-14
CA1314810C (en)	1993-03-23
ES554045A0 (es)	1987-10-16
DE3588078D1 (de)	1996-02-29
US4889798A (en)	1989-12-26
ATE133261T1 (de)	1996-02-15
EP0151492A3 (en)	1988-11-09
JPS60188095A (ja)	1985-09-25
AU3844485A (en)	1985-08-15
ES8802188A1 (es)	1988-04-01

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