NO912951L - Avidin-biotin-assistert immunoanalyse. - Google Patents

Avidin-biotin-assistert immunoanalyse.

Info

Publication number
NO912951L
NO912951L NO91912951A NO912951A NO912951L NO 912951 L NO912951 L NO 912951L NO 91912951 A NO91912951 A NO 91912951A NO 912951 A NO912951 A NO 912951A NO 912951 L NO912951 L NO 912951L
Authority
NO
Norway
Prior art keywords
antibody
immobilized
avidin
contacted
protein
Prior art date
Application number
NO91912951A
Other languages
English (en)
Other versions
NO912951D0 (no
Inventor
Thomas Thieme
Adolph Ferro
Jack H Fellman
Stefan Gavojdea
Original Assignee
Epitope Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epitope Inc filed Critical Epitope Inc
Publication of NO912951D0 publication Critical patent/NO912951D0/no
Publication of NO912951L publication Critical patent/NO912951L/no

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6891—Pre-targeting systems involving an antibody for targeting specific cells
    • A61K47/6897—Pre-targeting systems with two or three steps using antibody conjugates; Ligand-antiligand therapies
    • A61K47/6898—Pre-targeting systems with two or three steps using antibody conjugates; Ligand-antiligand therapies using avidin- or biotin-conjugated antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nanotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medical Informatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

En fremgangsmåte for deteksjon for screening og diagnostiske formål av anistoffer i et kroppsfluid ved hjelp av et antistoff-bindende protein, kovalentlig bundet til en porøs matrise, oppfanget i en gjennomsik-tig kolonne. Et testfluid inneholdende antistoff blir kontaktet med den protein-bundete matrisen hvorved proteinet reagerer med for å immobilisere eventuelle antistoffer tilstede i testfluidet. Et biotlnylert antigen av interesse blir kontaktet med det immobiliserte antistoffet, hvorved det biotinylerte antigenet bindes til det immobiliserte antistoffet. Avidin kovalentlig bundet til et enzym, blir kontaktet med det immobiliserte biotinylerte antigen-immobiliserte antistoff-proteinbundete matrisekomplekset, hvorved enzym-merket avidin bindes til det immobiliserte komplekset. Dette immobiliserte komplekset blir kontaktet med en substratoppløsning, hvori enzymet bundet til avidin katalyserer en reaksjon av sub-stratet, og produkserer et detekterbart reaksjonspro-dukt som korrelerer med tilstedeværelsen av antistoffet som skal bli detektert.
NO91912951A 1989-01-30 1991-07-29 Avidin-biotin-assistert immunoanalyse. NO912951L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30287789A 1989-01-30 1989-01-30
PCT/US1990/000378 WO1990008957A1 (en) 1989-01-30 1990-01-25 Avidin-biotin assisted immunoassay

Publications (2)

Publication Number Publication Date
NO912951D0 NO912951D0 (no) 1991-07-29
NO912951L true NO912951L (no) 1991-09-13

Family

ID=23169594

Family Applications (1)

Application Number Title Priority Date Filing Date
NO91912951A NO912951L (no) 1989-01-30 1991-07-29 Avidin-biotin-assistert immunoanalyse.

Country Status (10)

Country Link
EP (1) EP0455735B1 (no)
JP (1) JPH04503109A (no)
AT (1) ATE111604T1 (no)
AU (1) AU640635B2 (no)
CA (1) CA2045673A1 (no)
DE (1) DE69012552T2 (no)
DK (1) DK0455735T3 (no)
FI (1) FI913615A7 (no)
NO (1) NO912951L (no)
WO (1) WO1990008957A1 (no)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525338A (en) * 1992-08-21 1996-06-11 Immunomedics, Inc. Detection and therapy of lesions with biotin/avidin conjugates
US5198340A (en) * 1991-01-17 1993-03-30 Genentech, Inc. Assay for free igf-i, igf-ii, and gh levels in body fluids
US6087188A (en) * 1992-11-13 2000-07-11 Alk A/S Two-site immunoassay for an antibody with chemiluminescent label and biotin bound ligand
IL104384A (en) * 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
EP1181938B1 (en) 1993-04-23 2007-11-14 Wyeth Rapamycin conjugates and antibodies
US7279561B1 (en) 1993-04-23 2007-10-09 Wyeth Anti-rapamycin monoclonal antibodies
US5504091A (en) * 1993-04-23 1996-04-02 American Home Products Corporation Biotin esters of rapamycin
JPH11508036A (ja) * 1995-06-07 1999-07-13 アボツト・ラボラトリーズ 抗−ｈｉｖ−１または抗−ｈｉｖ−２抗体を検出するための改良されたハプテン−ペプチド結合物
DE19649390A1 (de) * 1996-11-29 1998-06-04 Boehringer Mannheim Gmbh Antigenspezifischer IgG-Nachweis
US6150115A (en) * 1997-04-04 2000-11-21 Seikagaku Kogyo Kabushiki Kaisha Quantitative determination method for heparan sulfate and diagnostic method using the same
US6379909B1 (en) 1998-06-24 2002-04-30 Alk-Abello A/S Method of detecting an antibody in a liquid sample
CN1189749C (zh) * 1998-06-24 2005-02-16 阿尔克-阿贝洛有限公司 检测液体样品中抗体的方法
WO2001020328A1 (fr) * 1999-09-13 2001-03-22 Nissui Pharmaceutical Co., Ltd. Procede et kit de detection ou de dosage d'une substance
ES2545526T3 (es) 2001-02-01 2015-09-11 Sigma-Aldrich Co. Llc Matrices de afinidad mejorada con visibilidad mejorada para aplicaciones de arrastre molecular e inmunoprecipitación
WO2002090961A2 (en) * 2001-05-03 2002-11-14 Immunetics, Inc. Systems and methods for detection of analytes in biological fluids
DE10153963A1 (de) * 2001-11-06 2003-05-22 Aventis Behring Gmbh Intellect Testsystem zur Untersuchung einer biologischen Flüssigkeit
CN111208290A (zh) * 2020-02-27 2020-05-29 江苏泽成生物技术有限公司 一种磁微粒化学发光法醛固酮测定试剂盒及使用方法
CN114236131B (zh) * 2021-11-05 2022-12-06 江苏省人民医院（南京医科大学第一附属医院） 一种检测甲状腺过氧化物酶抗体及其亚型的试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4301139A (en) * 1979-06-21 1981-11-17 Ames-Yissum Ltd. Multilayer column chromatography specific binding assay method, test device and test kit
US4425438A (en) * 1981-03-13 1984-01-10 Bauman David S Assay method and device
AU8594382A (en) * 1981-08-05 1983-02-10 F. Hoffmann-La Roche Ag Immunoassay using biotin-avidin-enzyme complex
JPS5856696A (ja) * 1981-09-30 1983-04-04 Amano Pharmaceut Co Ltd カラムを用いる酵素免疫測定法
US4535057A (en) * 1982-07-26 1985-08-13 Amf Incorporated Immunoassay employing monoclonal herpes simplex antibody and biotin-avidin detection system
SE458643B (sv) * 1984-12-07 1989-04-17 Pharmacia Ab Anordning med kolonnelement uppdelat i provparti och referenspatri

Also Published As

Publication number Publication date
CA2045673A1 (en) 1990-07-31
DE69012552T2 (de) 1995-04-20
ATE111604T1 (de) 1994-09-15
FI913615A0 (fi) 1991-07-29
WO1990008957A1 (en) 1990-08-09
AU5098690A (en) 1990-08-24
EP0455735A1 (en) 1991-11-13
EP0455735B1 (en) 1994-09-14
FI913615A7 (fi) 1991-07-29
JPH04503109A (ja) 1992-06-04
AU640635B2 (en) 1993-09-02
DK0455735T3 (da) 1994-12-12
NO912951D0 (no) 1991-07-29
DE69012552D1 (de) 1994-10-20

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