OA11712A - Novel process for the cryo-preservation of plants. - Google Patents
Novel process for the cryo-preservation of plants. Download PDFInfo
- Publication number
- OA11712A OA11712A OA1200100089A OA1200100089A OA11712A OA 11712 A OA11712 A OA 11712A OA 1200100089 A OA1200100089 A OA 1200100089A OA 1200100089 A OA1200100089 A OA 1200100089A OA 11712 A OA11712 A OA 11712A
- Authority
- OA
- OAPI
- Prior art keywords
- cryo
- plant
- medium
- preservation
- freezing
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 41
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 34
- 230000008569 process Effects 0.000 title claims abstract description 26
- 238000007710 freezing Methods 0.000 claims abstract description 40
- 230000008014 freezing Effects 0.000 claims abstract description 35
- 238000011419 induction treatment Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 71
- 229930006000 Sucrose Natural products 0.000 claims description 26
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 26
- 239000005720 sucrose Substances 0.000 claims description 26
- 244000299461 Theobroma cacao Species 0.000 claims description 19
- 240000007154 Coffea arabica Species 0.000 claims description 12
- 241000894007 species Species 0.000 claims description 12
- 241000227999 Coffea canephora Species 0.000 claims description 9
- 244000000626 Daucus carota Species 0.000 claims description 9
- 235000007460 Coffea arabica Nutrition 0.000 claims description 8
- 235000002187 Coffea robusta Nutrition 0.000 claims description 8
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 claims description 8
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 claims description 8
- 235000001046 cacaotero Nutrition 0.000 claims description 8
- 235000002767 Daucus carota Nutrition 0.000 claims description 7
- 210000002257 embryonic structure Anatomy 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 3
- 230000008929 regeneration Effects 0.000 abstract 1
- 238000011069 regeneration method Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 60
- 229910001868 water Inorganic materials 0.000 description 57
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 38
- 230000006698 induction Effects 0.000 description 25
- 229910052757 nitrogen Inorganic materials 0.000 description 19
- 239000007788 liquid Substances 0.000 description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 16
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 14
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 12
- 235000009470 Theobroma cacao Nutrition 0.000 description 11
- 239000000463 material Substances 0.000 description 10
- 235000021073 macronutrients Nutrition 0.000 description 9
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 9
- 238000003860 storage Methods 0.000 description 9
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 9
- 229960003495 thiamine Drugs 0.000 description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 8
- 235000019341 magnesium sulphate Nutrition 0.000 description 8
- 239000011785 micronutrient Substances 0.000 description 8
- 235000013369 micronutrients Nutrition 0.000 description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 7
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 7
- 239000001110 calcium chloride Substances 0.000 description 7
- 235000011148 calcium chloride Nutrition 0.000 description 7
- 229910001628 calcium chloride Inorganic materials 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 7
- 229960000367 inositol Drugs 0.000 description 7
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 7
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 7
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 7
- 229960003512 nicotinic acid Drugs 0.000 description 7
- 235000001968 nicotinic acid Nutrition 0.000 description 7
- 239000011664 nicotinic acid Substances 0.000 description 7
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 7
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 7
- 229940088594 vitamin Drugs 0.000 description 7
- 229930003231 vitamin Natural products 0.000 description 7
- 235000013343 vitamin Nutrition 0.000 description 7
- 239000011782 vitamin Substances 0.000 description 7
- 229920002148 Gellan gum Polymers 0.000 description 6
- 239000004471 Glycine Substances 0.000 description 6
- 239000007836 KH2PO4 Substances 0.000 description 6
- 229910004619 Na2MoO4 Inorganic materials 0.000 description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 6
- 230000001172 regenerating effect Effects 0.000 description 6
- 239000011684 sodium molybdate Substances 0.000 description 6
- 235000015393 sodium molybdate Nutrition 0.000 description 6
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000007774 longterm Effects 0.000 description 5
- 239000003375 plant hormone Substances 0.000 description 5
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
- 239000004472 Lysine Substances 0.000 description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 4
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 4
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 4
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 4
- 239000012526 feed medium Substances 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 230000003050 macronutrient Effects 0.000 description 4
- 235000010333 potassium nitrate Nutrition 0.000 description 4
- 229960004799 tryptophan Drugs 0.000 description 4
- 229940011671 vitamin b6 Drugs 0.000 description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 4
- 239000011686 zinc sulphate Substances 0.000 description 4
- 235000009529 zinc sulphate Nutrition 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 3
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- MKPCNMXYTMQZBE-UHFFFAOYSA-N 7h-purin-6-amine;sulfuric acid;dihydrate Chemical compound O.O.OS(O)(=O)=O.NC1=NC=NC2=C1NC=N2.NC1=NC=NC2=C1NC=N2 MKPCNMXYTMQZBE-UHFFFAOYSA-N 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- 239000006035 Tryptophane Substances 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 108010079058 casein hydrolysate Proteins 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
- 229960001669 kinetin Drugs 0.000 description 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- 230000030118 somatic embryogenesis Effects 0.000 description 2
- 235000019157 thiamine Nutrition 0.000 description 2
- 239000011721 thiamine Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 1
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241001338022 Daucus carota subsp. sativus Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012595 freezing medium Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 1
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000979 retarding effect Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 235000009492 vitamin B5 Nutrition 0.000 description 1
- 239000011675 vitamin B5 Substances 0.000 description 1
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Inorganic materials [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98118938 | 1998-10-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| OA11712A true OA11712A (en) | 2005-01-26 |
Family
ID=8232761
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| OA1200100089A OA11712A (en) | 1998-10-07 | 1999-09-27 | Novel process for the cryo-preservation of plants. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6783984B2 (de) |
| EP (1) | EP1119246B1 (de) |
| AT (1) | ATE221310T1 (de) |
| AU (1) | AU5982299A (de) |
| BR (1) | BR9915914A (de) |
| DE (1) | DE69902397T2 (de) |
| ES (1) | ES2180345T3 (de) |
| OA (1) | OA11712A (de) |
| WO (1) | WO2000019819A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2178365B1 (de) * | 2007-07-18 | 2011-05-18 | Dow AgroSciences LLC | Rettung von pflanzenzellkulturen und -suspensionen nach durch kryokonservierung hervorgerufenen beschädigungen |
| CN102657152B (zh) * | 2012-05-04 | 2014-06-25 | 南京林业大学 | 一种胚性材料的超低温冷冻保存方法 |
| WO2014164874A2 (en) | 2013-03-13 | 2014-10-09 | Becton, Dickinson And Company | Methods and compositions for modulation of amplification efficiency |
| CN107864953A (zh) * | 2017-11-23 | 2018-04-03 | 长沙湘资生物科技有限公司 | 一种天南星种子超低温保存方法 |
| CN109380216A (zh) * | 2018-10-30 | 2019-02-26 | 上海市农业生物基因中心 | 一种药用野生稻茎尖的超低温保存及恢复培养方法 |
| CN111513061B (zh) * | 2020-05-22 | 2022-05-03 | 上海市农业生物基因中心 | 一种矾根丛生芽超低温保存及恢复培养方法 |
| CN115380823B (zh) * | 2022-08-29 | 2023-05-19 | 安徽农业大学 | 一种延缓乌菜组培苗继代生长速率的方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0816488A1 (de) * | 1996-06-25 | 1998-01-07 | Societe Des Produits Nestle S.A. | Verfahren zur Konditionierung von in vitro kultivierten Pflanzengeweben |
| US5922929A (en) * | 1997-05-02 | 1999-07-13 | University Of Maryland At Baltimore County | Thermotolerance enhancing protein |
| US6143563A (en) * | 1997-05-20 | 2000-11-07 | Pioneer Hi-Bred International, Inc. | Cryopreservation of embryogenic callus |
-
1999
- 1999-09-27 DE DE69902397T patent/DE69902397T2/de not_active Expired - Fee Related
- 1999-09-27 AT AT99969997T patent/ATE221310T1/de not_active IP Right Cessation
- 1999-09-27 EP EP99969997A patent/EP1119246B1/de not_active Expired - Lifetime
- 1999-09-27 ES ES99969997T patent/ES2180345T3/es not_active Expired - Lifetime
- 1999-09-27 AU AU59822/99A patent/AU5982299A/en not_active Abandoned
- 1999-09-27 WO PCT/EP1999/007168 patent/WO2000019819A1/en not_active Ceased
- 1999-09-27 BR BR9915914-7A patent/BR9915914A/pt not_active Application Discontinuation
- 1999-09-27 OA OA1200100089A patent/OA11712A/en unknown
-
2001
- 2001-04-03 US US09/826,393 patent/US6783984B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1119246B1 (de) | 2002-07-31 |
| US20010041364A1 (en) | 2001-11-15 |
| BR9915914A (pt) | 2001-08-14 |
| ATE221310T1 (de) | 2002-08-15 |
| EP1119246A1 (de) | 2001-08-01 |
| DE69902397T2 (de) | 2003-03-20 |
| ES2180345T3 (es) | 2003-02-01 |
| US6783984B2 (en) | 2004-08-31 |
| AU5982299A (en) | 2000-04-26 |
| WO2000019819A1 (en) | 2000-04-13 |
| DE69902397D1 (de) | 2002-09-05 |
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