OA18720A - 2-H-indazole derivatives as cyclin-dependent kinase (CDK) inhibitors and therapeutic uses thereof. - Google Patents

Abstract

Indazole compounds of formula (I) <img file="OA18720A_A0001.tif"/> as cyclin-dependent kinase (CDK) and cellproliferation inhibitors, and therapeutic uses and methods of preparation thereof, are disclosed. These compounds, and pharmaceutically acceptable salts, solvates, prodrugs, and pharmaceutical compositions thereof, are useful for treating diseases and disorders associated with activity of cyclin-dependent kinases, in particular CDK4/6, including but not limited to various cancers and inflammation-related diseases or conditions.

Description

2-H-INDAZOLE DERIVATIVES AS CYCLIN-DEPENDENT KINASE (CDK) INHIBITORS AND THERAPEUTIC USES THEREOF

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims priority under 35 U.S.C. § 119(e) to U.S. Provisional Application No. 62/028,427, filed on July 24, 2014, which is incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

The présent invention is related to the field of compounds, compositions and methods for the treatment or prévention of a disease, disorder, or medical condition mediated through certain cyclin-dependent kinases (CDKs). The diseases include various cancers.

BACKGROUND OF THE INVENTION

Cyclin-dependent kinases are a family of protein kinases that regulate cell division and prolifération. Cell cycle progression is controlled by cyclins and their associated cyclin-dependent kinases, such as CDKl, CDK2, CDK3, CDK4 and CDK6, while other CDKs such as CDK7, CDK8 and CDK9 are critical to transcription. CDK binding to cyclins forms heterodimeric complexes that phosphorylate their substrates on serine and threonine residues, which in turn initiâtes events required for cell-cycle transcription and progression. Since uncontrolled cell prolifération is a hallmark of cancer, and most cancer cells exhibit deregulation of CDKs, inhibition of CDKs has emerged as a potential treatment for various cancers. Inhibitors with varying degrees of selectivity for CDKs hâve been reported; however, sélective CDK4/6 inhibitors are currently viewed as a promising class of potential anticancer or anti-inflammatory agents due to both the critical rôle of CDK4/6 in regulating cell prolifération and the toxic effects associated with inhibition of other members of the CDK family.

Recently, several types of aminopyrimidine dérivatives hâve been reported to be sélective CDK4/6 inhibitors. See, e.g., WO 2003/062236, WO 2007/140222, and US 2010/0160340. Each of these types of molécules contains a 2-aminopyrimidine moiety bound through the 2-amino group to an aryl or heteroaryl ring System. There remains a need to develop new CDK 4/6 inhibitors as novel anticancer and/or anti-inflammatory agents.

SUMMARY OF THE INVENTION

The present invention relates to 2-aminopyrimidine-substituted indazole dérivatives that are effective as sélective CDK inhibitors and useful in the treatment or prévention of diseases, disorders, or medical conditions mediated through certain CDKs, in particular CDK4 and CDK6, such as various types of cancers and inflammation-related conditions.

One aspect of the present invention is directed to a compound of formula (I):

or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein:

R¹ is selected from the group consisting of hydrogen, linear or branched Ci-Ce alkyl, and C3-C7 cycloalkyl;

R² and R³ are independently selected from the group consisting of hydrogen, linear or branched C;-Cg alkyl, C3-C7 cycloalkyl, and cycloalkylmethyl;

R⁴ is selected from the group consisting of hydrogen, halogen, linear or branched Ci-Cs alkyl, and C3-C7 cycloalkyl; and

R⁵ is hydrogen or halogen.

Another aspect of the present invention is directed to a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, and one or more pharmaceutically acceptable excipients, such as adjuvants, diluents, and/or carriers.

Another aspect of the present invention is directed to a method of treating a disease, disorder, or condition mediated through at least one of cyclin-dependent kinases (CDK), in particular CDK4, CDK6, or a combination thereof, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable sait, solvaté, or prodrug thereof.

Another aspect of the présent invention is directed to a method of treating a disease, disorder, or condition mediated through at least one of cyclin-dependent kinases (CDK), in particular CDK4, CDK.6, or a combination thereof, comprising administering to a subject in need thereof a therapeutically effective amount of a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, and one or more pharmaceutically acceptable excipients, such as adjuvants, diluents, and/or carriers.

In one embodiment, the diseases, disorders, or conditions associated with one or more cyclin-dependent kinases, in particular CDK4, CDK6, or a combination thereof, comprise cancers, which may include, but are not limited to, lung cancer, especially nonsmall cell lung cancer (NSCLC), breast cancer, prostate cancer, colorectal cancer, glioblastoma, mantel cell lymphoma, chronic myeloid leukemia and acute myeloid leukemia, and complications thereof. In another embodiment, the diseases, disorders, or conditions comprise the inflammation-related diseases and conditions, such as arthritis, e.g., rheumatic arthritis, and cystic fibrosis.

Another aspect of the invention is directed to a method of inhibiting cell prolifération comprising treating the cells with an effective amount of a compound of formula (I), or a sait, solvaté, prodrug, or composition thereof.

Another aspect of the invention is directed to a method of inhibiting a cyclindependent kinase (CDK), in particular CDK4, CDK6, or a combination thereof, comprising treating the kinase with an effective amount of a compound of formula (I), or a sait, solvaté, prodrug, or composition thereof.

Another aspect of the présent invention is directed to use of the compounds of this invention for the study of CDKs in bîological and pathological phenomena and for comparative évaluation of new kinase inhibitors.

Another aspect of the présent invention is directed to use of a compound of formula (I) according to any embodiments described herein, or a pharmaceutically acceptable sait, solvaté, prodrug, or composition thereof, in the manufacture of a médicament for treatment of a disease or disorder associated with a CDK activity. The CDK activity is preferably activity of CDK4, CDK6, or a combination thereof.

Still another aspect of the présent invention is directed to the methods of synthesizing the compounds of formula (I) as substantially disclosed and described herein.

Other aspects or advantages of the présent invention will be apparent to those skilled in the art in view of the following detailed description and daims in combination with the knowledge and skills generally known in the field.

DETAILED DESCRIPTION OF THE INVENTION

The présent invention provides novel 2-aminopyrimidine-substituted 2H-indazole dérivatives useful as CDK inhibitors.

In one aspect, the présent invention provides a compound of formula (I):

or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein:

R* is selected from the group consisting of hydrogen, Ci-Ce alkyl, and C3-C7 cycloalkyl;

R² and R³ are independently selected from the group consisting of hydrogen, linear or branched Ci-Cs alkyl, C3-C7 cycloalkyl, and C3-C7 cycloalkylmethyl;

R⁴ is selected from the group consisting of hydrogen, halogen, linear or branched Ci-Cs alkyl, and C3-C7 cycloalkyl; and

R⁵ is hydrogen or halogen.

In one embodiment of this aspect, R¹ is Ci-Cô alkyl.

In another embodiment of this aspect, R¹ is methyl, ethyl, propyl, or isopropyl.

In another embodiment of this aspect, R² is Ci-Cé alkyl, C3-C6 cycloalkyl, or C3-C6 cycloalkylmethyl.

In another embodiment of this aspect, R² is methyl, ethyl, propyl, isopropyl, cyclopropyl, cyclopentyl, cyclopropylmethyl, or cyclopentylmethyl.

In another embodiment of this aspect, R³ is Ci-Cé alkyl or C3-C6 cycloalkyl.

In another embodiment of this aspect, R³ is methyl, ethyl, propyl, isopropyl, or cyclopropyl.

In certain embodiments, the présent invention provides a compound of formula (I), wherein the R⁴ substituent is attached at the 7-position of the indazole moiety, as in formula (la):

or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein:

R¹ is selected from the group consisting of hydrogen, Ci-Cs alkyl, and C3-C7 cycloalkyl;

R² and R³ are independently selected from the group consisting of hydrogen, Ci-Cs alkyl, C3-C7 cycloalkyl, and cycloalkylmethyl;

R⁴ is selected from the group consisting of hydrogen, haiogen, Ci-Cg alkyl, and C₃C7 cycloalkyl; and

R⁵ îs hydrogen or haiogen.

In another embodiment of this aspect, R⁴ is hydrogen or haiogen.

In another embodiment of this aspect, R⁵ is hydrogen or fluoride.

In another embodiment of this aspect, R¹ is methyl or ethyl; R² is isopropyl, cyclopropyl, cyclopropylmethyl, or cyclopentyl; R³ is methyl or ethyl, R⁴ is hydrogen or fluoro, and R⁵ is hydrogen or fluoro.

In certain preferred embodiments of this aspect, the R⁴ substituent is attached at the 7-position of the indazole moiety, and R⁵ is fluorine, characterized by formula (Ib):

(Ib) wherein R⁴ is preferably hydrogen or halogen; and when R⁴ is a halogen, it is preferably chlorine or fluorine, more preferably fluorine.

In certain preferred embodiments of this aspect, the present invention provides a compound of formula selected from the group consisting of:

(le) or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R¹, R², and R³ are each defined in any of the embodiments described here.

In certain preferred embodiments of this aspect, the present invention provides the compounds listed in Table 1 (infra), and pharmaceutically acceptable salts, solvatés, and prodrugs thereof.

In another aspect, the present invention provides a pharmaceutical composition comprising a compound of formula (I), (la), (Ib), (le), (Id), or (le) according to any embodiments described here, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, and one or more pharmaceutically acceptable adjuvants, diluents, and/or carriers.

In another aspect, the present invention provides a method of treating a disease, disorder, or condition mediated through activity of at least one cyclin-dependent kinase (CDK), comprising administering to a subject in need thereof a therapeutically effective amount of the compound of formula (I), (la), (Ib), (le), (Id), or (le) according to any of the embodiments described herein, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof.

In one embodiment of this aspect, the present invention provides a method of treating a disease, disorder, or condition mediated through activity of at least one cyclindependent kinase (CDK), comprising administering to a subject in need thereof a pharmaceutical composition comprising a therapeutically effective amount of the compound of formula (I), (Ta), (Ib), (le), (Id), or (le) according to any of the embodiments described herein, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, and one or more pharmaceutically acceptable adjuvants, diluents, and/or carriers.

In one preferred embodiment of this aspect, the at least one CDK is CDK4, CDK6, or a combination thereof.

In another preferred embodiment of this aspect, the disease or disorder is a cancer or an inflammation-related disease or condition.

In another preferred embodiment of this aspect, the inflammation-related disease or condition is arthritis, such as rheumatic arthritis, or cystic fibrosis.

In another preferred embodiment of this aspect, the cancer is selected from, but not limited to, colorectal cancer, breast cancer, lung cancer, especially non-small cell lung cancer (NSCLC), prostate cancer, glioblastoma, mantel cell lymphoma (MCL), chronic myeloid leukemia (CML), acute myeloid leukemia (AML), and complications thereof.

In another embodiment of this aspect, the compound of the present invention may be administered to a subject in need thereof in combination with administration of a second therapeutic agent.

In another embodiment, the second therapeutic agent is a different CDK inhibîtor, a human epidermal growth factor receptor (e.g., HER2) inhibîtor, a serine/threonine kinase inhibîtor, such as a mammalian target of rapamycin (mTOR) inhibîtor, or an epidermal growth factor receptor (EGFR) inhibîtor.

In another aspect, the present invention provides a method of inhibiting cell prolifération, comprising treating the cells with an effective amount of the compound of formula (I) according to any of the embodiments described, or a sait, solvaté, prodrug, or composition thereof. The method of inhibiting cell prolifération can take place in vivo, e.g., inside the body of a subject, or in vitro, e.g., in a biological sample containing the proliférative cells of a subject.

In a preferred embodiment of this aspect, the proliférative cells are cancer cells, such as, but not limited to, cells of colorectal cancer, breast cancer, lung cancer, especially non-small cell lung cancer (NSCLC), prostate cancer, glioblastoma, mantel cell lymphoma (MCL), chronic myeloid leukemia (CML), acute myeloid leukemia (AML), or complications thereof.

In another aspect, the présent invention provides a method of inhibiting a cyclindependent kinase (CDK) comprising treating said kinase with an effective amount of a compound of formula (I) according to any embodiments described herein, or a sait, solvaté, prodrug, or composition thereof. The method of inhibiting CDK can take place in vivo, e.g., inside the body of a subject, or in vitro, e.g., in a biological sample containing the proliférative cells of a subject.

In a preferred embodiment of this aspect, the cyclin-dependent kinase is CDK4, CDK6, or a combination thereof.

In another aspect, the présent invention provides use of a compound of formula (I), (la), (Ib), (le), (Id), or (le) according to any embodiments described herein, or a pharmaceutically acceptable sait, solvaté, prodrug, or composition thereof, in the manufacture of a médicament for treatment of a disease or disorder associated with a CDK activity. The CDK activity is preferably activity of CDK4, CDK6, or a combination thereof.

In one embodiment of this aspect, the disease or disorder is selected from the group consisting of colorectal cancer, breast cancer, lung cancer, especially non-small cell lung cancer (NSCLC), prostate cancer, glioblastoma, mantel cell lymphoma (MCL), chronic myeloid leukemia (CML), and acute myeloid leukemia (AML).

In another embodiment of this aspect, the disease or disorder is an inflammationrelated disease or condition, such as arthritis, in particular rheumatic arthritis, or cystic fibrosis.

In another aspect, the présent invention provides a method of preparing a compound of formula (I), comprising a step of coupling intermediate E with intermediate G:

E G (D wherein R¹ through R⁵ are defined according to any of the embodiments described herein, and X³ is Cl, Br, or I.

In one embodiment of this aspect, the method further includes the steps of converting intermediate C to intermediate D and coupling the intermediate D with a pyrimidine compound H to form the intermediate E:

Boronation

Catalyst

Catalyst

wherein R* and R^y are independent alkyl, aryl, cycloalkyl, or altematively together form an alkylene group, each optionally substituted by one or more substituents independently selected from C1-C4 alkyl, halogen or phenyl; and wherein X¹, X², and X³ are each independently Cl, Br, or I, on condition that the intermediate D couples with the compound H selectively at the X² site over the X³ site, preferably having a higher than 15 90:10 selectivity, more preferably having a 95:5 selectivity, and most preferably exclusively at the X² site.

In one embodiment of this aspect, the method further includes the steps of converting starting material SI to intermediate A and converting the intermediate A to the intermediate C:

SI AC wherein X¹ is CI, Br, I, or MeSCh-; and wherein R² and R³ are as defined according to any of the embodiments described herein.

In another embodiment of this aspect, the method further includes converting the intermediate A to the intermediate C, altematively, comprises converting the intermediate A to an alcohol intermediate B followed by réduction of the alcohol intermediate B to form the intermediate C:

(aldéhyde or ketone)

A B C wherein R^2a and R^2b are each independently hydrogen, alkyl, cycloalkyl, or together form an alkylene group so that the group R^{2a RZb} formed in the intermediate C is R² as defined according to any of the embodiments described herein.

In one embodiment of this aspect, the method further includes a step of forming the intermediate G through coupling the pyridine aldéhyde compound S2 and the piperazine compound S3 to form an intermediate F, followed by converting the intermediate F to the intermediate G:

Reductive amination

Substitution or Réduction

F

G

S2 S3 wherein X⁴ is selected from the group consisting of Cl, Br, i, and -NOi; and wherein said converting the intermediate F to the intermediate G comprises replacing X⁴ with NH2 when X⁴ is Cl, Br, or I; or aiternatively reducing the nitro group (-NO2) to amino group (-NH₂) when X⁴ is -NO2.

Compounds provided by this invention are also useful for the study of kinases in biological and pathological phenomena, the study of transduction pathways mediated by such kinases, and the comparative évaluation of new kinase inhibitors.

Unless otherwise indicated, the term “alkyl,” as used herein, is intended to include both branched and straight-chain saturated aliphatic hydrocarbon groups containing l to 8 carbons, preferably l to 6, more preferably l to 4, carbons. The term encompasses, but is not limited to, methyl, ethyl, propyl, isopropyl, butyl, t-butyl, isobutyl, pentyl, hexyl, or the like.

Unless otherwise indicated, the term “alkylene,” as used herein, refers to a bivalent saturated aliphatic radical derived from an alkane by removal of two hydrogen atoms. Examples include, but are not limited to, methylene (-CH2-), ethylene (-CH2CH2-), propylene (-CH2CH2CH2-), or the like.

Unless otherwise indicated, the term “cycloalkyl”, as used herein alone or as a part of another group, includes saturated cyclic hydrocarbon radical having 3 to 8 carbons forming the ring. Examples include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

Unless otherwise indicated, the term “aryl”, as used herein alone or as part of another group, refers to monocyclic or bicyclic aromatic radical containing 6 to IO carbons in the ring portion (such as phenyl and naphthyl, including l-naphthyl and 2-naphthyl).

“Halo” or “halogen” as used herein, refers to fluoro (F), chloro (Cl), bromo (Br), and iodo (I).

Further, the alkyl, alkylene, cycloalkyl, and cycloalkylmethyl groups optionally can be independently further substituted with one or more, preferably l to 3, substituents independently selected from the group consisting of halogen and C1-C4 alkyl.

The compounds of the present invention are generally recognized as organic bases, which are able to react with acids, specifically pharmaceutically acceptable acids, to form pharmaceutically acceptable salts.

As used herein, the term pharmaceutically acceptable sait refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animais without undue toxicity, irritation, allergie response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. See, e.g., S. M. Berge et al., J. Pharm. Sci., 1977, 66, 1-19, which is incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycérophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, pivalate, propionate, stéarate, succinate, sulfate, tartrate, thiocyanate, ptoluenesulfonate, undecanoate, valerate salts, and the like. Preferred pharmaceutically acceptable salts include the hydrochloride salts.

The term “solvaté,” as used herein, means a physical association of a compound of this invention with a stoichiometric or non-stoichiometric amount of solvent molécules. For example, one molécule of the compound associâtes with one or more, preferably one to three, solvent molécules. It is also possible that multiple (e.g., 1.5 or 2) molécules of the compound share one solvent molécule. This physical association may include hydrogen bonding. In certain instances the solvatés will be capable of isolation as crystalline solid. The solvent molécules in the solvaté may be present in a regular arrangement and/or a non-ordered arrangement. Exemplary solvatés include, but are not limited to, hydrates, ethanolates, methanolates, and isopropanolates. Methods of solvation are generally known in the art.

The term ‘prodrug,” as used herein, refers to a dérivative of a compound that can be transformed in vivo to yield the parent compound, for example, by hydrolysis in blood. Common examples of prodrugs in the present invention include, but are not limited to, amide or phosphoramide forms of an active amine compound, for example, the compound of formula (II):

(H) wherein R⁶ is an acyl group (e.g., acetyl, propionyl, formyl, etc.) or phosphoryl [e.g., -P(=O)(OH)2] group; or alternatively, when R¹ or R³ in an active compound is hydrogen, the corresponding amide or phosphoramide compounds may serve as prodrugs. Such amide or phosphoramide prodrug compounds may be prepared according to conventional methods as known in the art.

When it is possible that, for use in therapy, therapeutically effective amounts of a compound of the present invention, or pharmaceutically acceptable salts or solvatés thereof, may be administered as the raw Chemical, it is possible to present the active ingrédient as a pharmaceutical composition. Accordingly, the disclosure further provides pharmaceutical compositions, which include any compounds of the present invention, or pharmaceutically acceptable salts or solvatés thereof, and one or more, preferably one to three, pharmaceutically acceptable carriers, diluents, or other excipients. The carrier(s), diluent(s), or other excipient(s) must be acceptable in the sense of being compatible with the other ingrédients of the formulation and not deleterious to the subject being treated.

The term “pharmaceutically acceptable,” as used herein, refers to the property of those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of patients without excessive toxicity, irritation, allergie response, or other problem or complication commensurate with a reasonable benefit/risk ratio, and are effective for their intended use.

Pharmaceutical formulations may be presented in unit dose forms containing a predetermined amount of active ingrédient per unit dose. Typically, the pharmaceutical compositions of this disclosure will be administered from once every l to 5 days to about l-5 times per day, or altematively, as a continuous infusion. Such administration can be used as a chronic or acute therapy. The amount of active ingrédient that may be combined with the carrier materials to produce a single dosage form will vary depending on the condition being treated, the severity of the condition, the time of administration, the route of administration, the rate of excrétion of the compound employed, the duration of treatment, and the age, gender, weight, and condition of the patient. Preferred unit dosage formulations are those containing a daily dose or sub-dose, as herein above recited, or an appropriate fraction thereof, of an active ingrédient. Generally, treatment is initiated with small dosages substantîally less than the optimum dose of the compound. Thereafter, the dosage is increased by small incréments until the optimum effect under the circumstances is reached. In general, the compound is most desirably administered at a concentration level that will generally afford effective results without causing substantial harmful or deleterious side effects.

When the compositions of this disclosure comprise a combination of a compound of the présent disclosure and one or more, preferably one or two, additional therapeutic or prophylactic agent, both the compound and the additional agent are usually présent at dosage levels of between about 10 to 150%, and more preferably between about 10 and 80% of the dosage normally administered in a monotherapy regimen.

Pharmaceutical formulations may be adapted for administration by any appropriate route, for example, by the oral (including buccal or sublingual), rectal, nasal, topical (including buccal, sublingual, or transdermal), vaginal, or parentéral (including subcutaneous, intracutaneous, intramuscular, intra-articular, intrasynovial, intrasternal, intrathecal, intralesional, intravenous, or intradermal injections or infusions) route. Such formulations may be prepared by any method known in the art of pharmacy, for example by bringing into association the active ingrédient with the carrier(s) or excipient(s). Oral administration or administration by injection are preferred.

Pharmaceutical formulations adapted for oral administration may be presented as discrète units such as capsules or tablets; powders or granules; solutions or suspensions in aqueous or non-aqueous liquids; edible foams or whips; or oil-in-water liquid émulsions or water-in-oil émulsions.

For instance, for oral administration in the form of a tablet or capsule, the active drug component can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as éthanol, glycerol, water, and the like. Powders are prepared by comminuting the compound to a suitable fine size and mixing with a similarly comminuted pharmaceutical carrier such as an edible carbohydrate, as, for example, starch or mannitol. Flavoring, preservative, dispersing, and coloring agent can also be présent.

Capsules are made by preparing a powder mixture, as described above, and filling formed gelatin sheaths. Glidants and lubricants such as colloïdal silica, talc, magnésium stéarate, calcium stéarate, or solid polyethylene glycol can be added to the powder mixture before the filling operation. A disintegrating or solubilizîng agent such as agar-agar, calcium carbonate, or sodium carbonate can also be added to improve the availability of the médicament when the capsule is ingested.

Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents, and coloring agents can also be incorporated into the mixture. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, and the like. Lubricants used in these dosage forms include sodium oleate, sodium chloride, and the like. Disintegrators include, without limitation, starch, methyl cellulose, agar, betonite, xanthan gum, and the like. Tablets are formulated, for example, by preparing a powder mixture, granulating or slugging, adding a lubricant and disintegrant, and pressing into tablets. A powder mixture is prepared by mixing the compound, suitable comminuted, with a diluent or base as described above, and optionally, with a binder such as carboxymethylcellulose, an aliginate, gelating, or polyvinyl pyrrolidone, a solution retardant such as paraffin, a résorption accelerator such as a quatemary sait and/or and absorption agent such as betonite, kaolin, or dicalcium phosphate. The powder mixture can be granulated by wetting with a binder such as syrup, starch paste, acadia mucilage, or solutions of cellulosic or polymeric materials and forcing through a screen. As an alternative to granulating, the powder mixture can be run through the tablet machine and the resuit is imperfectly formed slugs broken into granules. The granules can be lubrîcated to prevent sticking to the tablet forming dies by means of the addition of stearic acid, a stéarate sait, talc, or minerai oil. The lubricated mixture is then compressed into tablets. The compounds of the present disclosure can also be combined with a free flowing inert carrier and compressed into tablets directly without going through the granulating or slugging steps. A clear or opaque protective coating consisting of a sealing coat of shellac, a coating of sugar or polymeric material, and a polish coating of wax can be provided. Dyestuffs can be added to these coatings to distinguish different unit dosages.

Oral fluids such as solution, syrups, and élixirs can be prepared in dosage unit form so that a given quantity contains a predetermined amount of the compound. Syrups can be prepared by dissolving the compound in a suitably flavored aqueous solution, while élixirs are prepared through the use of a non-toxic vehicle. Solubilizers and emulsifiers such as ethoxylated isostearyl alcohols and polyoxyethylene sorbitol ethers, preservatives, flavor additive such as peppermint oil or natural sweeteners, or saccharin or other artificial sweeteners, and the like can also be added.

Where appropriate, dosage unit formulations for oral administration can be microencapsulated. The formulation can also be prepared to prolong or sustain the release, for example, by coating or embedding particulate material in polymers, wax, or the like.

It should be understood that in addition to the ingrédients particularly mentioned above, the formulations may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavoring agents.

The term “subject” or patient includes both humans and other mammalian animais, preferably humans.

The term “therapeutically effective amount refers to an amount of a compound or composition that, when administered to a subject for treating a disease, is sufficient to effect such treatment for the disease. A therapeutically effective amount can vary depending on, inter alia, the compound, the disease and its severity, and the âge, weight, or other factors of the subject to be treated. When applied to an individual active ingrédient, administered alone, the term refers to that ingrédient alone. When applied to a combination, the term refers to combined amounts of the active ingrédients that resuit in the therapeutic effect, whether administered in combination, serially, or simultaneousiy.

In some embodiments, the term treating or “treatment” refers to: (i) inhibiting the disease, disorder, or condition, i.e., arresting its development; (ii) relieving the disease, disorder, or condition, i.e., causing régression of the disease, disorder, and/or condition; or (iii) preventing a disease, disorder or condition from occurring in a subject that may be predisposed to the disease, disorder, and/or condition but has not yet been diagnosed as having it. Thus, in some embodiments, “treating or treatment refers to ameliorating a disease or disorder, which may include ameliorating one or more physical parameters, though maybe indiscemible by the subject being treated. In some embodiments, treating or treatment includes modulating the disease or disorder, either physically (e.g., stabilization of a discemible symptom) or physiologically (e.g., stabilization of a physical parameter) or both. In yet some embodiments, treating or treatment includes delaying the onset of the disease or disorder.

METHODS

Abbreviations

The following abbreviations may be used in this application:

Bipin? = bis(pinacolato)diboron

MeOH “ methanol

LDA = lithium diisopropylamide

LiHMDS = lithium bis(trimethylsilyl)amide [LiN(SiMe3)2]

Pd(dppf)Ch = [l,T-bis(diphenylphosphino)ferrocene]dichloropalladium(II)

Pdî(dba)3 = tris(dibenzylideneacetone)dipalladium(0)

Xantphos = 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene nBuaP = tri-n-butylphosphine

DCM = dichloromethane

THF = tetrahydrofuran;

DIEA = DIPEA = diisopropylethylamine;

sat. = saturated aqueous solution;

aq. = aqueous

FCC = flash column chromatography using silica;

TFA = trifluoroacetic acid;

r.t. = room température;

DMF = /VjV-dimethylformamide;

DMSO = dimethylsulfoxide;

DMA = ATV-dimethyÎacetamide;

EtOAc = ethyl acetate;

h = hour(s).

Chemical Synthesis

Synthesis of Compounds of Formula (I)

The synthesis of compounds of formula (I) is exemplified in the General Synthetic

Schemes l-4:

1. Synthesis ofthe indazole intermediate C (Scheme 1)

A suitable 5-halo-2-nitrobenzaldehyde starting material SI (X¹ = Cl, Br, or I) is allowed to react with a primary amine (R³NH₂) in the presence of a phosphine, e.g., tributylphosphine, to form the indazole dérivative A (Genung, N. E. et al. Org. Leît. 2014 16, 3114-3117), which in turn is deprotonated at the 3-position using a strong base, e.g., LDA, IS followed by reaction with an alkylation reagent R²X (X= e.g., Cl, Br, I, or methanesulfonate) to form the intermediate C with the desired R², R³, and R⁴ in place. Altematively, the deprotonated compound A can be allowed to react with an aldéhyde or ketone to form an alcohol adduct, which is reduced (e.g., by a dialkylsilane) to form the desired intermediate C.

Scheme 1

2. Synthesis of the pyrimidine-substituted indazole Intermediate E (Scheme 2) The intermediate C is allowed to undergo a boronation reaction in the presence of a catalyst (e.g., a palladium catalyst) to form the boronate intermediate D, which is allowed to couple with a halogen-substituted pyrimidine dérivative H to form a 5(pyrimidin-3-yl)-îndazole intermediate E.

Scheme 2

3. Synthesis of the 2-amino-5-piperazinylmethyl-pyridine intermediate G (Scheme 3)

A 6-halogen or 6-nitro substituted pyridine-3-carbaldehyde starting material S2 and a l -R’-substituted piperazine starting material S3 are allowed to undergo a reductive amination reaction to form a 2-amino-5-piperazinylmethyl-pyridine intermediate F, which is in tum converted to the 2-amino-5-piperazinylmethyl-pyridine intermediate G through substitution of the haiogen or réduction of nitro group on the pyridine ring.

Scheme 3

Reductive amination

Substitution or Réduction

4. Synthesis of compounds of formula (I) (Scheme 4)

Coupling of the pyrimidine-substituted indazole intermediate E with the 2-amîno5-piperazinemethyl-pyridine intermediate G in the presence of a catalyst (e.g., palladium catalyst) provides the compound of formula (I).

Scheme 4

EXAMPLES

The following non-limiting Examples further illustrate certain aspects of the present invention. These compounds are prepared according to the general Synthetic Schemes described above.

Exaniple 1. /V-(5-((4-Ethylpiperazin-yl)methyl)pyridin-2-yl-5-fluoro-4-(3-isopropyl-2methyl-2H-indazol-5-yl)pyrimidin-2-amine

Et

Synthesis of Compound 1

As an illustrated example, the synthesis of intermediates E, intermediate G, and Compound 1 are depicted in Schemes 5-7, respectively. In the following reaction schemes some spécifie reagents or reaction conditions are provided solely for better understanding, but such spécifie reagents or conditions are not intended to be limiting whatsoever. As a person of skill in the art would appreciate, any spécifie step of the reaction scheme could be accomplished using a variety of équivalent conditions in various aspects, such as reagents, température, catalysts, and/or solvents, etc.

Scheme 5

ch₃nh₂ π-BujP; 80°C i-PrOH

2. Me₂CO

1. LDA; -78“C;THF

Et₃SiH

TFA, DCM

Scheme 7

Intermediate A

Into a 500-mL round-bottom flask purged and maintained with an inert atmosphère of nitrogen were placed 5-bromo-2-nitrobenzaldehyde (30.0 g, 130.4 mmol, 1.0 equiv.), methanamine (71.5 mL, 1.1 equiv.), and propan-2-ol (300 mL). The resulting solution was stirred for 4 h at 80°C. The mixture was cooled to rt and tributylphosphine (98 mL, 3.0 equiv.) was added. The resulting solution was stirred for 12 h at 80°C and then extracted with 500 mL of ethyl acetate. The resulting mixture was washed sequentially with 300 mL of NH4CI (aq.) and 300 mL of brine. The mixture was dried over anhydrous sodium sulfate and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with ethyl acetate/petroleum ether (1:4). This resulted in 32 g (crude) of 5bromo-2-methyl-2H-mdazoie A as a red oil: MS m/z MH⁺= 211

Intermediate B

Into a 1 L 3-necked round-bottom flask purged and maintained with an inert atmosphère of nitrogen were placed 5-bromo-2-methyl-2H-indazole A (32.0 g, 128.9 mmol, 1.0 equiv., 85%) in tetrahydrofuran (300 mL). To the solution was added LDA (97.5 mL, 1.5 equiv., 2 M) at -78°C. The solution was stirred at 0-5°C for 10 min, then cooled to -78°C. To the solution was added propan-2-one (11.3 g, 194,7 mmol, 1.5 equiv.). The resulting solution was stirred for 12 h at 25°C. The reaction was then quenched with 100 mL of aqueous sodium bicarbonate. The resulting solution was extracted with 3x500 mL of ethyl acetate and the organic layers combined, dried with anhydrous Na2SO₄, and filtered. The filtrate was concentrated under vacuum, and the residue was applied onto a silica gel column and eluted with ethyl acetate/petroleum ether (1:10 to 1:2). This resulted in 20 g (58%) of 2-(5-bromo-2-methyl-2H-indazol-3-yl)propan-2-ol B as a yellow oil: MS m/zM⁺=269

Intermediate C

Into a 500-mL round-bottom flask were placed 2-(5-bromo-2-methyl-2H-indazol3-yl)propan-2-ol B (20.0 g, 74.3 mmol, 1.0 equiv.), triethylsilane (86.6 g, 744.4 mmol, 10.0 equiv.), trifluoroacetic acid (85.0 g, 752.0 mmol, 10.0 equiv.), and dichloromethane (200 mL), The resulting solution was stirred for 12 h at 25°C. The resulting mixture was concentrated under vacuum. The solution was adjusted to pH 8 with sodium bicarbonate (aq, 2 M). The resulting solution was extracted with 3x100 mL of ethyl acetate, and the organic layers combined and washed with 1x100 mL of brine. The mixture was dried with anhydrous Na2SO₄, filtered, and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with ethyl acetate/petroleum ether (1:20 to 1:10). This resulted in 8 g (43%) of 5-bromo-2-methyl-3-(propan-2-yl)-2H-indazole C as a yellow oil: MS [M+l ]⁺= 253 & 255 and [M+CH₃CN+H]⁺= 294 &296

Intermediate D

Into a 100 mL round-bottom flask purged and maintained with an inert atmosphère of nitrogen were placed 5-bromo-2-methyl-3-(propan-2-yl)-2H-indazole (1.9 g, 7.5 mmol, 1.0 equiv.), 4,4,5,5-tetramethyl-2-(tetramethyl-l ,3,2-dioxaborolan-2-yl)-l ,3,2dioxaborolane (2.3 g, 9.0 mmol, 1.2 equiv.), KOAc (1.47 g, 15.0 mmol, 2.0 equiv.), 1,4dioxane (40 mL), and water (10 mL). To the solution was added Pd(dppf)Ch (612 mg, 0.75 mmol, 0.1 equiv.). The resulting solution was stirred for 12 h at 90°C, and then cooled to room température and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with ethyl acetate/petroleum ether (1:5). This resulted in 2.5 g (crude) of 2-methyl-3-(propan-2-yl)-5-(tetramethyl-l,3,2-dioxaborolan-2-yl)-2Hindazole D as a white solid: MS m/z MH⁺=301

Intermediate E

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphère of nitrogen were placed 2-methyl-3-(propan-2-yl)-5-(tetramethyl-l,3,2-dioxaborolan-2yl)-2H-indazole (2.5 g, 7.5 mmol, 1.0 equiv., 90%), 2,4-dichloro-5-fluoropyrimidine (1.7 g, 9.9 mmol, 1.2 equiv.), potassium carbonate (2.3 g, 16.7 mmol, 2.0 equiv.), 1,4-dioxane (40 mL), and water (10 mL). To the solution was added Pd(dppf)Cl2 (680 mg, 0.83 mmol, 0.10 equiv.). The resulting solution was stirred for 2 h at 80°C, and then cooled to room température and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with ethyl acetate/petroleum ether (1:2). This resulted in 1.2 g (48%) of 5-(2-chloro-5-fluoropyrimidin-4-yl)-2-methyl-3-(propan-2-yl)-2H-indazole E as an offwhite solid: MS m/z MH ⁺— 305 ; ’H NMR (300 MHz, CDCI3, ppm): ô 1.58 (d, 6H), 3.48-3.57 (m, 1 H), 4.19 (s, 3H), 7.24 (d, 1H), 8.03-8.07 (m, 1H), 8.47 (d, 1H), 8.68 (s, 1H).

Intermediate F

NaBH(OAc)₃ (14.4 g, 68.0 mmol, 1.1 equiv.) was added portionwise over 30 min to a solution of jV-ethyl piperazine (7.7 g, 67.5 mmol, 1.1 equiv.) and 6-bromopyridine-3carbaldehyde (11.6 g, 62.5 mmol, 1.0 equiv.) in 150 mL of methylene chloride. The reaction mixture was stirred for 48 h, then diluted with CH2CI2 and excess 2N NaOH (aq.). The layers were separated, and the aqueous phase was extracted with CH2CI2. The

CH2CI2 extracts were combined, dried (Na^SOi), and concentrated to yield l-(6-bromopyridin-3-ylmethyl)-4-ethyl-piperazine F as an oil: MS m/z MH⁺ = 285

Intermediate G

Lithium bis(trimethylsilyl)amide (LiHMDS) (IM solution in THF, 12.7 mL, I2.7 mmol, 1.2 equiv) was added to a solution of intermediate F (3.0 g, 10.6 mmol, l.O equiv.), dicyclohexylphosphinobiphenyl (0.227 g, 0.64 mmol, 0.06 equiv.), and Pd2(dba)₃ (0.291 g, 0.32 mmol, 0.03 equiv.) in 15 mL of THF. The mixture was heated at 50 °C for 3 h, then cooled to room température, and filtered through a Dicalite® filtering medium. The filtrate was concentrated and the residue was taken up in CH2CI2 and extracted twice with 10% HCl (aq.). The HCl extracts were combined and washed with EtOAc, and the aqueous phase was basified with l .0 N NaOH, and then extracted four times with CH2CI2. The organic extracts were combined, dried over Na₂SC>4 and concentrated to yield l .2 g of 5(4-ethyl-piperazinyl-l -ylmethyl)-pyridin-2-ylamine G as a tan solid: MS m/z MH⁺ =221.

Compound 1

A mixture of 5-(2-chloro-5-fluoropyrimidin-4-yl)-2-methyl-3-(propan-2-yl)-2Hindazole (E) (700 mg 2.3 mmol, 1.3 equiv.), 5-((4-ethylpiperazin-l-yl)methylpyridin-2amine (G) (389 mg, 1.8 mmol, 1.0 equiv.), CS2CO3 (2.3 g, 7.2 mmol, 4.0 equiv.), Pd2(dba)3 (0.164 g, 0.18 mmol, 0.1 equiv.) and Xantphos (0.104 g, 0,18 mmol, 0.1 equiv.) in 10 mL of 1,4-dioxane was degassed with Nz, then heated while stirring at 110°C for 4h. The mixture was cooled to room température, then filtered through a Dicalite® filtering medium, and the filter pad was washed thoroughly with CH2CI2. The filtrate was concentrated, and the residue was purified by column chromatography on silica gel using a CH2Ch-2% NHa/MeOH gradient to afford 0.475 g of V-(5-((4-ethylpiperaziny|)methyl)pyridÎn-2-yl-5-fluoro-4-(3-isopropyl-2-methyl-2H-indazol-5-yl)pyrimidin-2amine (1). MS m/z MH⁺ 489; Ή NMR (300 MHz, CDClj, ppm)·. Ô 0.97 (t, 3H,J= 7.1 Hz), 1.51 (d, 2H, J= 7.0 Hz), 2.27-2.51 (overlapping m, 10 H), 3.33 (s, 3H), 3.54-3.68 (m, 1H), 4.15 (s, 3H), 7.64-8.71 (overlapping m, 7H), 10.00 (s, 1H).

Compounds 1 and other selected examples (Compounds 2-31) of the present invention are listed in Table l, ail of which are or can be prepared according to the methods described above.

Table 1. Selected examples of the compounds

Example	Structure	Name
1	N^F A Uvri H IL 1 /-N Me—ζ Me Me	jV-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-5fluoro-4-(3-isopropyl-2methyl-2H-indazol-5yl)pyrimidin-2-amine
2	m b 4 // y— z IZ M*x z y \=< cd / x / /=\ ή z> Φ r \ '/ /Z-·^ \ CD	2V-(5-((4-ethylpiperazin-1 yl)methyl)pyrîdin-2-yl)-5fluoro-4-(7-fluoro-3isopropyl-2-methyl-2Hindazol-5-yl)pyrimidin-2amine
3	LL -y ,Ξ 1 // \__1 ω \ /ν'2 / <υ c~f Z—/ // Ά 5 X LU	Ar-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-4(7-fluoro-3-isopropyl-2methyI-2H-indazol-5yl)pyrimidin-2-amine
4	CÜ LL· I \ ZI Z—Z // Ά o X LU	4-(3-cyclopentyl-2-methyl- 2H-indazoI-5-yl)-y-(5-((4- ethylpiperazin-1- yl)methyl)pyridin-2-yl)-5- fluoropyrimidin-2-amine
5	rn rtx O d TZ b AM, n>	4-(3-cyclopentyl-7-fluoro2-methyl-2H-indazol-5-yl)jV-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine

6	Η IL 1 / ΝMe	4-(3-cyclopenty!-7'fluoro2-methyl-2H-indazol-5-yl)2V-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2yl)pyrimidin-2-amine
7	m b i IZ \--z- ·,ύ ' s' z (D	4-(3-cyciopropyl-2-methyl2H-Î ndazol-5-yI )-N-( 5-((4ethylpiperazin-1yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
8	m b 4 // V— z IZ b Xz c Φ	4-(3 -cyclopropyl-7-fluoro2-methyi-2H-indazol-5-yl)Ar-(5-((4-ethylpiperazin-l yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
9	eX’XUTb H IL Λ / N /—< Me	4-(3-cyclohexyl-2-methyl2H-indazol-5-yl)-M-(5-((4ethylpiperazin-1yl)methyi)pyridin-2-yl)-5fluoropyrimidin-2-amine
10	m b // y— z ΣΕΖ Z I /=^ Xz ' *n <T>	4-(3-cycîohexyl-7-fluoro- 2-methyl-2H-indazol-5-yl)ïV-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
11	l H l 1 Ύ> /“N. \ Me	5-fluoro-4-(3-isopropyl-2- methyl-2H-indazol-5-yl)- V-(5-((4- isopropylpiperazin-1 - yl)methyl)pyridin-2- yl)pyrimidin-2-amine

12	ύ€	Γΐ	Χϊ χχτ Η	F x^F τ τ Ύτ /— N —< Me	5-fluoro-4-(7-fluoro-3isopropyl-2-methyl-2Hindazo!-5-yl)-?/-(5-((4isopropylpiperazin-1 yl)methyl)pyridin-2yl)pyrimidin-2-amine
13		η	Ο ςί: rS	„F /- N —< Me	4-(3-cyclopentyl-2-methyl2H-indazol-5-yl)-5-fluoro- ΛΓ-(5-((4isopropylpiperazin-1 yl)methyl)pyridin-2yl)pyrimidin-2-amine
14	-/	η	Μ ΏΣΖ Η\ ZU	.F ν/χ/ X X Ύ? /-N ''-f Me	4- (3-cyclopentyl-7-fluoro2-methyl-2H-indazol-5-yl)- 5- ίΊαοΓθ-?/-(5-((4isopropylpiperazin-1 yl)methyl)pyridin-2yl)pyrimidin-2-amîne
15			0 ÜCZ Ô	S ιΓχ / N —f Me	5-fluoro-4-(3-isopropyl-2methyl-2H-indazol-5-yl)7V-(5-((4-propylpiperazin1 -yl)methyl)pyridin-2yl)pyrimidin-2-amine
16	/χΑ	''''Ν''''	Ύ^Ν Ν^ Η	.F Lx^x-F X x X> /-N Ç Me	5-fluoro-4-(7-fluoro-3isopropyl-2-methyl-2Hindazol-5-yl)-/V-(5-((4propylpiperazin-1yl)methyl)pyridin-2yl)pyrimidin-2-amine
17	N		V^N \ί^·Ν^Ν Η	s^F Sp /- N f^\ Me	4-(3-cyclopentyl-2-methyl- 2H-indazol-5-yl)-5-fluoroÀL(5-((4-propylpiperazin1 -yl)methyl)pyridin-2yl)pyrimidin-2-amine

18	^OXuXv H ü 1 / n Me	4- (3-cyclopentyl-7-fluoro- 2-methyl-2H-indazoI-5-yl)- 5- fluoro-N-(5-((4propylpiperazin-1 yl)methyl)pyridin-2yl)pyrimidin-2-amine
19	φ ΐ LL \==/^LU C 2 M ZI P Q LU	4-(3-ethyl-2-methyl-2Hindazol-5-yl)W-(5-((4ethylpiperazin-1yI)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
20	<D LL f LL \=/ lu Q ZI ~z.—Z // 7 5 x^ LU	4-(3-ethyl-7-fluoro-2methyl-2H-indazol-5-yl)jV-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-5fluoropyrimidtn-2-amine
21	m b d ΞΖ J /=( Π φ	4-(3-(sec-butyl)-2-methyl- 2H-indazol-5-yl)-A-(5-((4ethylpiperazin-1yl)methyl)pyrid!n-2-yl)-5fluoropyrimidin-2-amine
22	φ LL -, ,Ξ X__ζΓ'Ζ IL /=^ / Cf -Ζ.Τ. Q LLJ	4-(3-(sec-butyl)-7-fluoro-2methyl-2H-indazol-5-yl)N-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-5- fluoropyrimidin-2-amine
23	m X 0 d / /=/ HH m r-+	4-(2-ethyl-3-isopropyl-2Hindazol-5-yl)-jV-(5-((4ethylpiperazin-1yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine

24	Et	C		a	'N' H	N'	•NX	XF Y	^F A r—N Et	4-(2-ethyl-7-fluoro-3isopropyI-2H-indazol-5yl)-7V-(5-((4ethylpiperazin-1yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
25	Et	i		a	‘N' H	N'	n ‘N	XF	A >—N Et	4-(3-cyclopropyl-2-ethyl- 2H-indazol-5-yl)-y-(5-((4- ethylpiperazin-1- yl)methyl)pyridin-2-yl)-5- fluoropyrimidin-2-amine
26	Et	i		o.	'N' H	N'	J] ’N	.F	^F A λ—N Et	4-(3-cyclopropyl-2-ethyl-7fluoro-2H-indazol-5-yl)-2V(5-((4-ethy Ipiperazin-1 yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
27	Et	i		a	'N' H	N'	’N		^.F A Et	4-(3-(cyciopropylmethyt)2-methyl-2H-indazo!-5-yl)~ 7V-(5-((4-ethyipiperazin-1 yl)methyl)pyridin-2-yl)-5fluoropyrimidin'2-amine
28	Et	i		a	'N' H	N	'N^	.F Y	A λ-Ν Me	4-(3-(cyclopropylmethyl)7-fluoro-2-methyl-2Hindazol-5-yl)-A^-(5-((4ethylpiperazin-1yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
29	Et	i		a	'N' H	N	'N	^F Y	A Λ—N	4-(3-cyclopropyl-2-ethyl-7fluoro-2H-indazol-5-yl)-jV(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2yl)pyrimidin-2-amîne
									Me

30	EtN^		Q ϋ	F V? /-N /y Me	4-(3-(sec-butyl)-2-methyl- 2H-indazol-5-yl)-M(5-((4ethylpiperazin-1yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine
31	A	p	Q Γ) H	F Ύ T y— n / ( Me	4-(3-(sec-butyl)-7-fluoro-2methyl-2H-indazol-5-yl)A-(5-((4-ethylpiperazin-1 yl)methyl)pyridin-2-yl)-5fluoropyrimidin-2-amine

BIOLOGICAL ASSAYS

Compounds of the formula l are novel CDK4/6 inhibitors that hâve been or can be evaluated for their activity according to the procedures described below.

Biochemical Assay

Cyclin Dl was added to freshly prepared reaction buffer [20 mM Hepes (pH 7.5), 10 mM MgCh, l mM EGTA, 0.02% Brîj 35, 0.02 mg/mL BSA, 0.1 mM NasVCU, 2 mM DTT, l% DMSO]. CDK.4 or CDK6 was delivered to the substrate solution and gently mixed. Compounds were tested in a I0-dose IC50 mode with 3-fold dilution starting at 10 μΜ. The compounds, diluted in DMSO, were added into the kinase reaction mixture by Acoustic technology (Echo550; nanoliter range) and incubated for 20 minutes at room température. 33P-ATP (l μΜ) was added to the reaction mixture to initiate the reaction. The kinase reaction was incubated for 2 hours at room température. The reactions were spotted onto P8l ion exchange paper and the kinase activity detected by filter-binding method. Curves were fitted to a nonlînear régression curve using a four parameter logistic équation (GraphPad Prism). Under these conditions, an IC50 value of <1.0 nM was determined for 1 in both CDK4 and CDK6 assays.

Cell Assay

The MCF7 human tumor cells were seeded in a clear polystyrène 96-well microculture plate (Corning® Costar® 96-well fiat bottom plate, Cat.# 3997) in a total volume of 90 gL/well.

After 24 hours of incubation in a humidified incubator at 37 °C with 5% CChand 95% air, 10 pL of 10X, serially diluted 1 in growth medium was added to each well in duplicate (10 point dose response). After 72 hours of culture in a humidified incubator at 37°C, in an atmosphère of 5% CChand 95% air, the plated cells and Cell Titer-Glo® (Promega G7571) reagents were brought to room température to equilîbrate for 30 minutes. 100 pL of Cell Titer-Glo® reagent was added to each well. The plate was shaken for two minutes and then left to equilîbrate for ten minutes. The medium/Cell Titer Glo® reagent was transferred to a white polystyrène 96well microculture plate (Corning® Costar® 96-well fiat bottom plate, Cat.# 3917) before reading luminescence on the Tecan GENios microplate reader. Percent inhibition of cell growth was calculated relative to untreated control wells. Ail tests were performed in duplicate at each concentration level. The ICso value for the test agents was estimated using Prism 6.05 by curve-fîtting the data using the following four parameter-logistic équation:

Top - Bottom _ T = —--------+ Bottom (\ ⁿ x/ I /ic / i'-'SOj where Top is the maximal % of control absorbance, Bottom is the minimal % of control absorbance at the highest agent concentration, Y is the % of control absorbance, Xis the agent concentration, ICso is the concentration of agent that inhibits cell growth by 50% compared to the control cells, and n is the slope of the curve. Under these conditions, an ICso value of <2 μΜ was determined for 1.

Table 2 summarizes biochemical and MCF7 cell-based data for the three reported CDK4/6 inhibitors 32, 33 and 34. The data was obtained under assay conditions described herein.

Table 2. Biochemical and cell data for known inhibitors of CDK4/6

32 ICjo (μΜ)	33 1CM (μΜ)	34 ICso (μΜ)
MCF7 «IL·	10	< 2	10
CDK4 biochemical assay	<0.005	< 0.001	< 0 001
CDK6 biochem ical assay	< 0 002	<0.001	< 0.002

It will be understood by those of skill in the art that numerous and various modifications can be made to the compounds, compositions, and/or methods of the présent invention without departing from the spirit of the invention. Therefore, the various 5 embodiments of the présent invention described herein are illustrative only, and are not intended to limit the scope of the invention in any way. Ail references cited herein are incorporated by reference in their entirety.

Claims (32)

1. What is claimed is:

   1. A compound of formula (I)

   (I) or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein:

   R¹ is hydrogen, Ci-Cs alkyl, or C3-C7 cycloalkyl;

   R² and R³ are each independently hydrogen, Ci-Cs alkyl, C3-C7 cycloalkyl, or C₃C7 cycloalkylmethyl;

   R⁴ is hydrogen, halogen, Ci-Cs alkyl, or C3-C7 cycloalkyl; and

   R⁵ is hydrogen or halogen.
2. 2. The compound of claim 1, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R¹ is Ci-Cô alkyl.
3. 3. The compound of claim 1, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R¹ is methyl, ethyl, propyl, or isopropyl.
4. 4. The compound of any one of daims l to 3, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R² is Ci-Cô alkyl, C3-C6 cycloalkyl, or C3-C6 cycloalkylmethyl.
5. 5. The compound of any one of daims 1 to 3, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R² is methyl, ethyl, propyl, isopropyl, cyclopropyl, cyclopentyl, cyclopropylmethyl, or cyclopentylmethyl.
6. 6. The compound of any one of daims 1 to 5, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R³ is Ci-Cô alkyl or C3-C6 cycloalkyl.
7. 7. The compound of any one of claims l to 5, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R³ is methyl, ethyl, propyl, isopropyl, or cyclopropyl.
8. 8. The compound of any one of claims l to 7, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R⁴ is located at the 7-position of the indazole ring, characterized by formula la:

   (la)
9. 9. The compound of any one of claims l to 8, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R⁴ is hydrogen or halogen.
10. 10. The compound of any one of claims l to 9, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R^s is hydrogen or fluoro.
11. 11. The compound of any one of claims l to 9, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, wherein R¹ is methyl or ethyl; R² is isopropyl, cyclopropyl, cyclopropylmethyl, or cyciopentyl; R³ is methyl or ethyl; R⁴ is hydrogen or fluoro; and R⁵ is hydrogen or fluoro.
12. 12. The compound of any one of claims 1 to 11, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, selected from the group consisting of:

    (le) (Id)

    (le)
13. 13. The compound of claim l, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, selected from the list of Table l.
14. 14. A pharmaceutical composition comprising a compound of any one of daims l to 13, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, and one or more pharmaceutically acceptable adjuvants, diluents, and/or carriers.
15. 15. A method of treating a disease, disorder, or condition mediated through activity of at least one cyclin-dependent kinase (CDK), comprising administering to a subject in need thereof a therapeutically effective amount of the compound of any one of daims l to 13, or a pharmaceutically acceptable sait, solvaté, or prodrug, or a pharmaceutical composition of daim 14.
16. 16. The method of daim I5, wherein said at least one CDK is CDK4, CDK6, or a combination thereof.
17. 17. The method of daim 15, wherein said disease or disorder is cancer.
18. 18. The method of daim 17, wherein said cancer is selected from the group consisting of colorectal cancer, breast cancer, lung cancer, especially non-small cell lung cancer (NSCLC), prostate cancer, glioblastoma, mantel cell lymphoma (MCL), chronic myeloid leukemia (CML), and acute myeloid leukemia (AML), and complications thereof.
19. 19. The method of claim 15, wherein said disease or disorder is an inflammation-related disease or condition selected from arthritis and cystic fibrosis.
20. 20. The method of any of daims 15 to 19, in combination with administering to said subject a second therapeutic agent.
21. 21. The method of daim 20, wherein said second therapeutic agent is a different CDK inhibitor, HER2 inhibitor, mTOR inhibitor, or EGFR inhibitor.
22. 22. A method of inhibiting cell prolifération comprising treating proliférative cells with an effective amount of a compound according to any one of claims l to 13, or a sait, solvaté, or prodrug thereof, or a composition of claim I4.
23. 23. A method of inhibiting a cyclin-dependent kinase (CDK) comprising treating said kinase with an effective amount of a compound of any one of claims l to I3, or a sait, solvaté, prodrug, or composition thereof.
24. 24. The method of claim 23, wherein said cyclin-dependent kinase is CDK.4, CDK6, or a combination thereof.
25. 25. Use of a compound according to any one of claims l to 13, or a pharmaceutically acceptable sait, solvaté, or prodrug thereof, or a composition of claim 14, in the manufacture of a médicament for treatment of a disease or disorder associated with a CDK activity.
26. 26. The use of claim 25, wherein said CDK is CDK4, CDK6, or a combination thereof.
27. 27. The use of claim 25 or 26, wherein said disease or disorder is selected from the group consisting of colorectal cancer, breast cancer, lung cancer, especially non-small cell lung cancer (NSCLC), prostate cancer, glioblastoma, mantel cell lymphoma (MCL), chronic myeloid leukemia (CML), acute myeloid leukemia (AML), and inflammationrelated diseases and conditions.
28. 28. A method of preparing a compound of formula (I), comprising a step of coupling intermediate E with intermediate G:

    wherein R¹ through R⁵ are defined as in any one of claims 1 to 13, and X³ is Cl, Br, orl.
29. 29. The method of daim 28, further comprising the steps of converting intermediate C to intermediate D and coupling the intermediate D with a pyrimidine compound H to form the intermediate E:

    wherein R^x and R^y are independent alkyl, aryl, cycloalkyl, or alternatively together form an alkylene group, each optionally substituted by one or more substituents independently selected from Ci-C₄ alkyl, halogen or phenyl; and wherein X¹, X², and X³ are each independently Cl, Br, or I, on condition that the intermediate D couples with the compound H selectively at the X² site over the X³ site.
30. 30. The method of claim 28 or 29, further comprising the steps of convertine starting material SI to intermediate A and converting the intermediate A to the intermediate C:

    SI A C wherein X is Cl, Br, 1, or MeSCh-; and R² and R³ are defined as in claim 28 or 29.
31. 31. The method of claim 30, wherein said converting the intermediate A to the intermediate C, alternatively, comprises converting the intermediate A to an alcohol intermediate B followed by réduction of the alcohol intermediate B to form the intermediate C.

    wherein R^2a and R^2b are each independently hydrogen, alkyl, cycioalkyl, or together form an alkylene group so that the group R^{2a R2b} formed in the intermediate C 5 is R² as defined in claim 30.
32. 32. The method of any one of daims 28 to 31, further comprising a step of forming the intermediate G through coupling the pyridine aldéhyde compound S2 and the piperazine compound S3 to form an intermediate F, followed by converting the intermediate F to the intermediate G:

    G

    S2 S3 F wherein X⁴ is selected from the group consisting of Cl, Br, I, and -NOz; and wherein said converting the intermediate F to the intermediate G comprises replacing -X⁴ with -NH2 when X⁴ is Cl, Br, or i; or reducing the nitro group (-NO2) to

    15 amino group (-NH2) when X⁴ is NO2.