PH19584A - A method for purification of recombinant mature human interleukin-2 - Google Patents

A method for purification of recombinant mature human interleukin-2

Info

Publication number
PH19584A
PH19584A PH31644A PH31644A PH19584A PH 19584 A PH19584 A PH 19584A PH 31644 A PH31644 A PH 31644A PH 31644 A PH31644 A PH 31644A PH 19584 A PH19584 A PH 19584A
Authority
PH
Philippines
Prior art keywords
purification
human interleukin
mature human
recombinant mature
recombinant
Prior art date
Application number
PH31644A
Inventor
Hsiang-Fu Kung
Shigeko Yamazaki
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of PH19584A publication Critical patent/PH19584A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Homogeneous recombinant human interleukin-2 is prepared by extraction of IL-2 from the cell membrane components of transformed microorganisms after lysis and purification of the extract by chromatography. <IMAGE>
PH31644A 1983-12-23 1984-12-26 A method for purification of recombinant mature human interleukin-2 PH19584A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US56498683A 1983-12-23 1983-12-23

Publications (1)

Publication Number Publication Date
PH19584A true PH19584A (en) 1986-05-26

Family

ID=24256731

Family Applications (1)

Application Number Title Priority Date Filing Date
PH31644A PH19584A (en) 1983-12-23 1984-12-26 A method for purification of recombinant mature human interleukin-2

Country Status (12)

Country Link
EP (1) EP0147819B1 (en)
JP (2) JPH0728753B2 (en)
AT (1) ATE76905T1 (en)
AU (1) AU580276B2 (en)
CA (1) CA1340853C (en)
DE (1) DE3485759D1 (en)
DK (1) DK174501B1 (en)
IE (1) IE57914B1 (en)
IL (1) IL73882A (en)
NZ (1) NZ210634A (en)
PH (1) PH19584A (en)
ZA (1) ZA849910B (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925919A (en) * 1984-04-25 1990-05-15 Roland Mertelsmann Purified interleukin 2
US4992271A (en) * 1982-09-23 1991-02-12 Cetus Corporation Formulation for lipophilic IL-2 proteins
US4853332A (en) * 1982-10-19 1989-08-01 Cetus Corporation Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of biologically active proteins
JPS60115528A (en) * 1983-11-28 1985-06-22 Takeda Chem Ind Ltd Human interleukin-2 protein, its production and pharmacological composition containing the same
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4908433A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Uses of interleukin-2
US4908434A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Process for preparing purified interleukin-2
WO1986002068A1 (en) * 1984-09-26 1986-04-10 Takeda Chemical Industries, Ltd. Mutual separation of proteins
IL76360A0 (en) * 1984-09-26 1986-01-31 Takeda Chemical Industries Ltd Mutual separation of proteins
DE3580590D1 (en) * 1985-05-29 1990-12-20 Green Cross Corp MANUFACTURING PROCESS FOR HETEROGENEOUS PROTEINS.
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
EP0227833B1 (en) * 1985-07-16 1991-01-16 Green Cross Corporation Process for preparing hetero-protein
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C (en) * 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US5162503A (en) * 1987-05-19 1992-11-10 Hoffmann-La Roche, Inc. Purification of interleukin-2 by receptor-affinity chromatography
EP0337243A1 (en) * 1988-04-14 1989-10-18 F. Hoffmann-La Roche Ag Process for purifying recombinant human interleukin-2
ZA898139B (en) * 1988-11-17 1990-08-29 Hoffmann La Roche Recombinant interleukin-2 hybrid proteins
IT1262981B (en) * 1992-09-09 1996-07-23 Tecnogen Scpa PROCEDURE FOR PURIFYING THE BIG ENDOTELINE PROTEIN
US7144999B2 (en) * 2002-11-23 2006-12-05 Isis Pharmaceuticals, Inc. Modulation of hypoxia-inducible factor 1 alpha expression
JP2006141263A (en) * 2004-11-18 2006-06-08 Kyoto Univ Method for producing (R) -hydroxynitrile lyase
RU2565553C2 (en) * 2013-04-09 2015-10-20 Общество с ограниченной ответственностью "Стратегия" Method of obtaining interleukin-2 preparation, interleukin-2 preparation and pharmaceutical immunomodulating preparation
ES2779977T3 (en) * 2014-07-10 2020-08-21 Univ Zuerich Immunostimulatory monoclonal antibodies against human interleukin 2
HK1257518A1 (en) 2016-01-11 2019-10-25 Novartis Ag Immune-stimulating humanized monoclonal antibodies against human interleukin-2, and fusion proteins thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58159420A (en) * 1982-03-18 1983-09-21 Ajinomoto Co Inc Purification method of interleukin 2
DE3377363D1 (en) * 1982-03-31 1988-08-18 Ajinomoto Kk Gene coding for interleukin-2 polypeptide, recombinant dna carrying said gene, cell lines possessing the recombinant dna,and method for producing interleukin-2 using said cells
EP0092163B1 (en) * 1982-04-20 1988-09-28 Sloan-Kettering Institute For Cancer Research Purification of interleukin 2
JPS58198293A (en) * 1982-05-12 1983-11-18 Shionogi & Co Ltd Production of immunoregulator such as interleukin
JPS59144719A (en) * 1983-02-03 1984-08-18 Japan Found Cancer Gene coding interleukin-2 polypeptide, recombined dna substance containing gene, and preparation of interleukin-2 using live cell strain containing dna substance and cell
ZA842025B (en) * 1983-03-21 1984-11-28 Hoffmann La Roche Interleuken-2
JPS60115528A (en) * 1983-11-28 1985-06-22 Takeda Chem Ind Ltd Human interleukin-2 protein, its production and pharmacological composition containing the same
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
DE3419995A1 (en) * 1984-05-29 1985-12-05 Hoechst Ag, 6230 Frankfurt GENE TECHNOLOGICAL METHOD FOR PRODUCING HUMAN INTERLEUKIN-2 AND MEANS FOR CARRYING OUT THIS METHOD

Also Published As

Publication number Publication date
CA1340853C (en) 1999-12-21
EP0147819B1 (en) 1992-06-03
IE57914B1 (en) 1993-05-19
DE3485759D1 (en) 1992-07-09
JPH07233082A (en) 1995-09-05
JPH0728753B2 (en) 1995-04-05
JP2864996B2 (en) 1999-03-08
JPS60172295A (en) 1985-09-05
AU3699584A (en) 1985-07-04
DK174501B1 (en) 2003-04-28
NZ210634A (en) 1989-05-29
EP0147819A3 (en) 1987-09-23
EP0147819A2 (en) 1985-07-10
IE843320L (en) 1985-06-23
ZA849910B (en) 1985-09-25
IL73882A (en) 1989-05-15
AU580276B2 (en) 1989-01-12
DK612484A (en) 1985-06-24
ATE76905T1 (en) 1992-06-15
IL73882A0 (en) 1985-03-31
DK612484D0 (en) 1984-12-19

Similar Documents

Publication Publication Date Title
PH19584A (en) A method for purification of recombinant mature human interleukin-2
PH24804A (en) Expression,processing and secretion of heterologous proteins by yeast
IT1164626B (en) PROCEDURE FOR THE RECOVERY OF HUMAN IFN-B FROM TRANSFORMED PRODUCER MICROORGANISMS
IL72988A0 (en) Preparation of immune interferon
DE3378790D1 (en) Fabrication method of membrane structure
HUT45432A (en) Method and apparatus for producing tube membrane consists of ring elements
AU1615983A (en) Bipolar membrane
IL121897A0 (en) A process for purifying a crude solution of active recombinant human interleukin-4
AU555495B2 (en) Apparatus for gasification of carbon
PL140608B2 (en) Method for continuqus pirometallurgical conversion of lead-copper matte and apparatus therefor
EP0175274A3 (en) Apparatus for separation of blood components
AU651444B2 (en) Method of manufacture of an electrophoresis gel slab
AU590712B2 (en) Immune interferon and method for its purification
AU5793386A (en) Apparatus for membrane separation
DK163670C (en) PROCEDURE FOR SEPARATION AND EXTRACTION OF NOSIHEPTID
JPS5730504A (en) Method and device for concentrating substance by filtration of membrane
CU21670A1 (en) Obtainment procedure of ethylic alcohols by fermentative way