PL208366B1 - Pharmaceutical compounds for auxiliary treatment of bird infections - Google Patents

Description

Description of the invention

The subject of the invention is pharmaceutical compositions for the auxiliary treatment of infectious diseases in birds, characterized by a disinfecting effect on the urinary tract, kidneys and liver, and a protective effect on the central nervous system, also having immunostimulating properties.

Certain viral diseases, such as paramyxovirosis in birds, damage the central nervous system, kidneys and liver. Destroying the virus directly is very difficult and is currently not used in therapy. One of the therapeutic strategies is to protect the organs, most often damaged by infection, and to generally strengthen the condition of the animal and its immune system. This strategy is used with high doses of vitamin B1, B vitamins, vitamin C and diuretics, and sulfur amino acids are also administered.

The invention relates to pharmaceuticals for the auxiliary treatment of infectious diseases in birds in the form of a mixture of herbal extracts.

The essence of the invention is that the mixture consists of ethanolic extract of knotweed herb in the amount of 5-6% w / v, ethanol extract of birch leaves in the amount of 6-7% w / v and acetate extract of bearberry leaves in the amount of 2 -5% w / v, dissolved up to 88% w / v of 30% aqueous ethanol.

The essence of the invention also consists in the fact that the mixture consists of ethanolic extract of knotweed herb in the amount of 5-6% w / v, ethanol extract of birch leaves in the amount of 6-7% w / v and acetate extract of bearberry leaves in the amount of 2-5% w / v and Echinacea herb ethanol in an amount of 5-10% w / v, dissolved up to 83% w / v of 30% aqueous ethanol solution.

The essence of the invention also consists in the fact that the mixture consists of knotweed ethanol extract in the amount of 5-6% w / v, ethanolic extract of birch leaves in the amount of 6-7% w / v, acetate extract of bearberry leaves in the amount of 2 -5% w / v, from the ethanol extract of Echinacea herb in the amount of 5-10% w / v and from the ethanol extract of milk thistle in the amount of 5-15% w / v, dissolved up to 76% w / v 30% aqueous ethanol solution.

The mixtures of these extracts may also contain B vitamins, vitamin C, as well as N-acetyl-L-cysteine or methionine and combinations thereof.

During the research, it was unexpectedly found that the above-mentioned plant extract mixtures have a comprehensive protective effect on the kidneys, liver and central nervous system of birds and strengthen their immune system and are characterized by a mild diuretic, non-irritating effect on the kidneys. These agents are especially recommended for the treatment of paramyxovirosis, a viral disease in birds which often causes damage to the kidneys, central nervous system and liver. In the treatment of paramyxovirosis, in order to protect these organs, additional vitamin preparations, such as vitamin B1, other B vitamins, vitamin C, and other preparations that stabilize the liver parenchyma, diuretics and disinfectants of the urinary tract, are used. The pharmaceutical compositions mentioned in accordance with the invention are also used in the auxiliary treatment of all diseases in which it is necessary to have a disinfecting effect on the urinary tract and protect the liver, kidneys and the central nervous system. These agents can be used after prior treatment with antibiotics or in parallel with them or alone for the treatment or prevention of paramyxovirosis in particular.

The aforementioned action of the pharmaceuticals according to the invention was confirmed in clinical trials in a flock of racing pigeons of several dozen birds, including adults and young animals, aged 1 to 3 months.

The indications for the use of the above-mentioned agents were clinical symptoms suggesting the presence of an infection of the urinary tract in birds. The clinical symptoms included general depletion of the majority of birds in the flock, impaired coordination of movements when turning the neck, difficulties in maintaining body balance, difficult food intake, reluctance to fly, watery diarrhea with a large admixture of urate. As a result of the diseases, losses in the herd reached about 5% of individuals within a week. The pathological examination of the dead animals showed changes in the urinary system in the form of numerous small petechiae on the surface of the kidneys, significant dilatation and filling of the ureters with deposits of uric acid salts. Moreover, the changes also indicated inflammation of the upper respiratory tract and catarrhal inflammation of the small intestines.

The pharmaceutical compositions according to the invention were administered after a 5-day prior therapy of the birds with a fluoroquinolone antibiotic. The therapy with their use lasted about 30 days. During this period, the birds received 40 μΐ of these pharmaceuticals per animal daily with drinking water. During the period of their use, no changes were noticed indicating their side effects. Birds took water with medication willingly. No falls in the herd were recorded during this period. The general condition of the birds during the period of administration of the above-mentioned agents was good. Gradually, an improvement in the consistency of the faeces and a decreased urate content were observed. On the basis of clinical studies of the pharmaceutical compositions according to the invention, their effect can be assessed as clearly positive.

The subject matter of the invention is illustrated in the following examples.

Example I. Composition of a pharmaceutical

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 10 g Echinacea herb extract 5 g

Bearberry leaf extract 3 g

30% aqueous ethanol solution 96.3

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

Vitamin C 0.1 g

N-acetyl-L-cysteine 0.1 g

Methionine 0.2 g

The final product contains approximately 14.6 mg / ml total phenols and approximately 2.3 mg / ml flavonoids as determined by colorimetry, and approximately 3.5 mg / ml hydroquinone and approximately 1 mg / ml attributein as determined by high performance liquid chromatography - HPLC. The pharmaceutical agent given in this example is especially recommended for the adjunctive treatment of paramyxovirosis. The pharmaceutical is prepared as follows:

Knotweed herb and birch leaves are extracted with a solution of ethanol in water for 3 days at 50 ° C, then the ethanol-water extracts are concentrated to dryness.

The bearberry leaves are extracted with a solution of ethyl alcohol in water for 3 days at 50 ° C, the extract obtained is concentrated under reduced pressure and then made up with water, then optionally hydrolyzed in 0.2 M HCl at 80 ° C for 60 minutes, cooled and adjusted to pH = 7 with sodium hydroxide solution. The solution prepared in this way is extracted with ethyl acetate, and the obtained acetate extract is concentrated to dryness, purified from residual ethyl acetate, dissolving it in 96% ethanol, and then concentrated to dryness at the temperature of 80 ° C.

Echinacea herb and milk thistle seeds are extracted with an aqueous solution of ethanol.

The milk thistle extract is concentrated to dryness under reduced pressure.

Echinacea herb extract, after the ethanol has been partially evaporated, is left in the refrigerator for 24 hours in order to precipitate the chlorophyll and then concentrated to dryness under reduced pressure.

In order to obtain the final product, all the extracts described above are dissolved in a 30% aqueous solution of ethyl alcohol at a dose of 96.3 g, which is the equivalent of 100 ml, and mixed. Vitamins B12, B2, B6 and C as well as N-acetyl-L-cysteine and methionine are added to the obtained mixture.

P r z x l a d II. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Bearberry leaf extract 5 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example. The agent is used as an astringent, disinfectant and diuretic.

PL 208 366 B1

P r x l a d III. Composition of the pharmaceutical agent:

Knotweed herb extract 6 g

Birch leaf extract 7 g

Bearberry leaf extract, hydrated 3 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The use of hydrolyzed bearberry extract does not require the use of alkalizing mineral waters during therapy, which enhances its antibacterial effect.

P r x l a d IV. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 10 g

Bearberry leaf extract 3 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The agent with the extract of milk thistle fruit has a beneficial protective effect on liver cells.

Example V. Composition of a pharmaceutical agent:

Knotweed herb extract 6 g

Birch leaf extract 7 g

Milk thistle fruit extract 9 g

Bearberry leaf extract, hydrolyzed 4 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The extract contains hydrolyzed bearberry extract, which excludes the need to use alkaline mineral waters during therapy.

P r x l a d VI. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 10 g

Purple Echinacea herb extract 5 g

Bearberry leaf extract 3 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The agent differs from those described above in that it contains an extract of echinacea with immunostimulating properties.

Example VII. Composition of the pharmaceutical agent:

Knotweed herb extract 6 g

Birch leaf extract 7 g

Milk thistle fruit extract 5 g

Purple Echinacea herb extract 5 g

Bearberry leaf extract, hydrolyzed 4 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The agent contains a hydrolyzed extract of bearberry leaves, which increases its antibacterial effect in vitro.

P r x l a d VIII. Composition of the pharmaceutical agent:

Knotweed herb extract 6 g

Birch leaf extract 6 g

Milk Thistle Fruit Extract 12 g Purple Echinacea Herb Extract 5 g

Bearberry leaf extract 5 g

Vitamin B1 0.4 g 30% aqueous ethanol solution 96.3 g

This agent is intended for the treatment of paramyxovirosis.

The addition of vitamin B1 provides a protective effect on the central nervous system.

PL 208 366 B1

P r x l a d IX. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 10 g

Purple Echinacea herb extract 20 g

Bearberry leaf extract, hydrolyzed 3 g

Vitamin B1 0.4 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The agent contains a hydrolyzed extract of bearberry, which excludes the need to use alkalizing mineral waters during therapy.

Example X. Pharmaceutical composition:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 10 g

Purple Echinacea herb extract 5 g

Bearberry leaf extract 5 g

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

This measure differs from the examples given in that the addition of three B vitamins affects the production of energy in cellular respiration, which eliminates the demyelination and inflammation of the peripheral nerves, axonal edema and degeneration of neuromuscular plates in birds, and facilitates the synthesis of neutotransmitters such as serotonin and norepinephrine, and the synthesis of sphingolipids needed to form the myelin sheath. In addition, it reduces irritability, nervousness and depression as well as peripheral nerve neuropathy and convulsions in the case of greater deficiencies, which can cause uneven gait, running combined with wing flapping, seizures and death.

P r z x l a d XI. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 12 g

Purple Echinacea herb extract 8 g

Bearberry leaf extract, hydrolyzed 3 g

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

This measure does not require the use of alkalizing mineral waters.

P r x l a d XII. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 12 g

Purple Echinacea herb extract 8 g

Bearberry leaf extract 3 g

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

Vitamin C 0.1 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The content of vitamin C, in addition to its many beneficial functions in the organisms of birds, predestines this composition for the auxiliary treatment of infectious diseases, especially colds, which ensures that the disease lasts less and is milder.

PL 208 366 B1

P r x l a d XIII. Composition of the pharmaceutical agent

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 15 g

Purple Echinacea Extract 5 g

Bearberry leaf extract, hydrolyzed 3 g

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

Vitamin C 0.1 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

The agent differs from that given in Example 12 by its stronger in vitro antimicrobial activity and in that it does not require the use of alkalizing mineral waters during therapy.

P r z k ł a d XIV. Composition of the pharmaceutical agent:

Knotweed herb extract 5 g

Birch leaf extract 6 g

Milk thistle fruit extract 10 g

Purple Echinacea herb extract 5 g

Bearberry leaf extract 3 g

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

Vitamin C 0.1 g

N-acetyl-L-cysteine 0.2 g

Methionine 0.2 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

Due to the content of sulfur-containing amino acids with a protective effect on the liver, in combination with milk thistle extract, it gives a greater and wider protective effect. P r x l a d XV. Composition of the pharmaceutical agent:

Knotweed herb extract 6 g

Birch leaf extract 7 g

Milk thistle fruit extract 15 g

Purple Echinacea herb extract 5 g

Bearberry leaf extract, hydrolyzed 3 g

Vitamin B1 0.4 g

Vitamin B2 0.2 g

Vitamin B6 0.3 g

Vitamin C 0.1 g

N-acetyl-L-cysteine 0.2 g

Methionine 0.2 g

30% aqueous ethanol solution 96.3 g

The extracts are prepared and mixed in the same way as in the first example.

This agent differs from that given in Example 14 in that its antimicrobial activity is stronger and does not require the use of urine alkalising mineral water.

Claims (3)

Patent claims 1. Pharmaceutical agent for auxiliary treatment of infectious diseases in birds, in the form of a mixture of herbal extracts, characterized in that the mixture consists of ethanolic extract of knotweed herb in the amount of 5-6% w / v, ethanolic extract of birch leaves in the amount of 6 - 7% w / v and bearberry leaf acetate extract in an amount of 2-5% w / v, dissolved up to 88% w / v of a 30% aqueous ethanol solution. PL 208 366 B1 2. Pharmaceutical agent for auxiliary treatment of infectious diseases in birds, in the form of a mixture of herbal extracts, characterized in that the mixture consists of ethanolic extract of knotweed herb in the amount of 5-6% w / v, ethanolic extract of birch leaves in the amount of 6 -7% w / v and acetate extract of bearberry leaves in the amount of 2-5% w / v and ethanol extract of Echinacea herb in the amount of 5-10% w / v, dissolved in an amount of up to 83% w / v 30% aqueous ethanol solution. 3. A pharmaceutical agent for auxiliary treatment of infectious diseases in birds, in the form of a mixture of herbal extracts, characterized in that the mixture consists of ethanolic extract of bird knotweed in the amount of 5-6% by weight / volume, ethanolic extract of leaves birch trees in the amount of 6-7% w / v, acetate extract of flour leaves in the amount of 2-5% w / v, from ethanolic extract of Echinacea herb in the amount of 5-10% w / v and an ethanol extract of milk thistle fruit in an amount of 5-15% w / v, dissolved up to 76% w / v of a 30% aqueous ethanol solution.