PL2265931T5 - Sposób i urządzenie do lokalizacji pojedynczych cząsteczek barwnika w mikroskopii fluorescencyjnej - Google Patents

Sposób i urządzenie do lokalizacji pojedynczych cząsteczek barwnika w mikroskopii fluorescencyjnej

Info

Publication number
PL2265931T5
PL2265931T5 PL09722568T PL09722568T PL2265931T5 PL 2265931 T5 PL2265931 T5 PL 2265931T5 PL 09722568 T PL09722568 T PL 09722568T PL 09722568 T PL09722568 T PL 09722568T PL 2265931 T5 PL2265931 T5 PL 2265931T5
Authority
PL
Poland
Prior art keywords
localization
dye molecules
fluorescent microscopy
single dye
microscopy
Prior art date
Application number
PL09722568T
Other languages
English (en)
Other versions
PL2265931T3 (pl
Inventor
Paul Lemmer
Cristoph Cremer
David Baddeley
Heinz Eipel
Original Assignee
Univ Heidelberg Ruprecht Karls
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43234230&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2265931(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/EP2008/009671 external-priority patent/WO2009115108A1/en
Application filed by Univ Heidelberg Ruprecht Karls filed Critical Univ Heidelberg Ruprecht Karls
Publication of PL2265931T3 publication Critical patent/PL2265931T3/pl
Publication of PL2265931T5 publication Critical patent/PL2265931T5/pl

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
PL09722568T 2008-03-19 2009-03-13 Sposób i urządzenie do lokalizacji pojedynczych cząsteczek barwnika w mikroskopii fluorescencyjnej PL2265931T5 (pl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102008015051 2008-03-19
PCT/EP2008/009671 WO2009115108A1 (en) 2008-03-19 2008-11-14 A method and an apparatus for localization of single dye molecules in the fluorescent microscopy
EP09722568.4A EP2265931B9 (en) 2008-03-19 2009-03-13 A method and an apparatus for localization of single dye molecules in the fluorescent microscopy
PCT/EP2009/001840 WO2009115244A1 (en) 2008-03-19 2009-03-13 A method and an apparatus for localization of single dye molecules in the fluorescent microscopy

Publications (2)

Publication Number Publication Date
PL2265931T3 PL2265931T3 (pl) 2014-01-31
PL2265931T5 true PL2265931T5 (pl) 2017-10-31

Family

ID=43234230

Family Applications (1)

Application Number Title Priority Date Filing Date
PL09722568T PL2265931T5 (pl) 2008-03-19 2009-03-13 Sposób i urządzenie do lokalizacji pojedynczych cząsteczek barwnika w mikroskopii fluorescencyjnej

Country Status (2)

Country Link
EP (1) EP2265931B9 (pl)
PL (1) PL2265931T5 (pl)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3596232T3 (da) * 2017-03-12 2023-08-07 Ilytica Llc Digitale molekylanalyser
US20210271060A1 (en) * 2018-07-03 2021-09-02 c/o THE UNITED STATES OF AMERICA, AS REPRSENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SER Systems and Methods for Improved Axial Resolution in Microscopy Using Photoswitching and Standing Wave Illumination Techniques
WO2021207656A1 (en) 2020-04-09 2021-10-14 Ilytica Llc Assays with induced aggregation for enhanced sensitivity
DE102020127320B3 (de) * 2020-10-16 2022-01-27 Abberior Instruments Gmbh Verfahren und Fluoreszenzmikroskop zur Ortsbestimmung einzelner fluoreszierender Farbstoffmoleküle durch adaptive Abtastung
DE102020127385B3 (de) * 2020-10-16 2022-03-10 Abberior Instruments Gmbh Verfahren und Vorrichtung zur lichtmikroskopischen Multiskalen-Aufnahme biologischer Proben
DE102021000060A1 (de) 2021-01-07 2022-07-07 Christoph Cremer Verfahren der Ring-Array-Beleuchtungsmikroskopie mit großen Arbeitesabständen und hoher Auflösung
CN113049552B (zh) * 2021-03-07 2022-08-05 天津大学 基于外泌体检测和单分子荧光漂白技术的muc1蛋白定量检测方法
WO2023006176A1 (en) * 2021-07-26 2023-02-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Localizing a singularized fluorophore molecule by continuously moving a focused light beam around the molecule
CN115950863B (zh) * 2022-12-05 2025-07-11 深圳高性能医疗器械国家研究院有限公司 单分子荧光检测系统
CN116930140B (zh) * 2023-07-31 2024-03-12 之江实验室 一种基于金刚石nv色心的单分子定位方法、设备及介质
CN117269126A (zh) * 2023-08-30 2023-12-22 华南师范大学 一种基于事件引导的高靶向性荧光相关光谱方法及其装置
CN120235755A (zh) * 2023-12-28 2025-07-01 中国科学院生物物理研究所 基于光开关分子的去噪去卷积超分辨成像方法和装置

Also Published As

Publication number Publication date
EP2265931B9 (en) 2017-08-30
EP2265931A1 (en) 2010-12-29
PL2265931T3 (pl) 2014-01-31
EP2265931B1 (en) 2013-05-22
EP2265931B2 (en) 2017-01-04

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